FastQCFastQC Report
Thu 26 May 2016
SRR936870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097079
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.4206326991019413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69700.332367068670279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46380.22116477252406802No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35470.16914002762890668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122850.028.7762681
GGTATCA86950.027.3235471
ATCAACG149000.023.4003413
TCAACGC149650.023.3271054
TATCAAC150700.023.2956392
CAACGCA151400.023.0574725
AACGCAG153900.022.7121546
GTACATG77650.022.457491
GAGTACT86600.022.10150512-13
TACATGG78150.021.5187842
AGTACTT89150.021.30950412-13
AGAGTAC128550.021.24295610-11
ACATGGG77650.020.7993533
GTACTTT90800.020.7668414-15
ACGCAGA170550.020.439187
CGCAGAG170650.020.3158848
GCAGAGT180150.018.7963899
CATGGGG36000.018.3384464
ATCACGC1300.007563270618.270023
ACTTTTT108450.017.56289516-17
Produced by FastQC (version 0.11.5)