##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936870_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2097079 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19193172980131 33.0 31.0 34.0 31.0 34.0 2 32.27092017038938 33.0 31.0 34.0 30.0 34.0 3 32.40872041539684 34.0 31.0 34.0 31.0 34.0 4 36.0818309658339 37.0 35.0 37.0 35.0 37.0 5 35.9936468773947 37.0 35.0 37.0 35.0 37.0 6 36.02311643958096 37.0 35.0 37.0 35.0 37.0 7 35.975039566940495 37.0 35.0 37.0 35.0 37.0 8 35.95580757806454 37.0 35.0 37.0 35.0 37.0 9 37.6569857406421 39.0 37.0 39.0 35.0 39.0 10-11 37.550484984113616 39.0 37.0 39.0 35.0 39.0 12-13 37.48374334014122 39.0 37.0 39.0 35.0 39.0 14-15 38.71733754426991 40.0 38.0 41.0 35.0 41.0 16-17 38.63629672511145 40.0 38.0 41.0 34.0 41.0 18-19 38.66942542460251 40.0 38.0 41.0 34.5 41.0 20-21 38.76321254468716 40.0 38.0 41.0 34.5 41.0 22-23 38.70439287218078 40.0 38.0 41.0 34.0 41.0 24-25 38.62246081335038 40.0 38.0 41.0 34.0 41.0 26-27 38.46377294322245 40.0 38.0 41.0 34.0 41.0 28-29 38.31721408683221 40.0 38.0 41.0 34.0 41.0 30-31 38.273672093421375 40.0 38.0 41.0 34.0 41.0 32-33 38.20218813883501 40.0 38.0 41.0 34.0 41.0 34-35 38.080963807276696 40.0 38.0 41.0 33.5 41.0 36-37 37.92042407558323 40.0 37.0 41.0 33.0 41.0 38-39 37.788028967912034 40.0 37.0 41.0 33.0 41.0 40-41 37.57975188345313 40.0 37.0 41.0 33.0 41.0 42-43 37.364773573146266 40.0 37.0 41.0 32.5 41.0 44-45 37.13908059734516 40.0 37.0 41.0 31.5 41.0 46-47 36.80804800391402 40.0 36.0 41.0 31.0 41.0 48-49 36.46588063682866 39.0 36.0 41.0 30.0 41.0 50-51 36.2416818822753 39.0 36.0 41.0 30.0 41.0 52-53 36.03265971382099 39.0 35.0 40.0 29.5 41.0 54-55 35.76896816953486 39.0 35.0 40.0 28.5 41.0 56-57 35.52737212093584 38.5 35.0 40.0 28.0 41.0 58-59 35.19564236731186 38.0 34.5 40.0 27.5 41.0 60-61 34.874831611017036 38.0 34.0 40.0 27.0 41.0 62-63 34.54932265308078 37.5 34.0 40.0 26.0 41.0 64-65 34.15217047140332 37.0 33.5 39.5 26.0 41.0 66-67 33.72505899873109 36.0 33.0 39.0 25.5 40.0 68-69 33.28789044189561 36.0 32.5 39.0 24.5 40.0 70-71 32.88715303524569 35.0 32.0 38.0 24.0 40.0 72-73 32.49124901827733 35.0 32.0 38.0 22.5 40.0 74-75 32.012944672089134 35.0 31.0 37.0 21.5 39.0 76-77 30.545593656700582 33.5 29.5 35.5 20.0 38.0 78-79 31.253060566626246 34.0 31.0 36.0 20.5 38.5 80-81 30.95052785326638 34.0 31.0 36.0 18.0 37.5 82-83 30.738067807650545 34.0 31.0 35.0 18.5 37.0 84-85 30.397848865016527 34.0 30.5 35.0 18.0 36.5 86-87 30.02831319182539 34.0 30.0 35.0 14.5 36.0 88-89 29.646226966175334 34.0 30.0 35.0 8.5 36.0 90-91 29.199484378032494 34.0 29.0 35.0 4.5 35.0 92-93 28.773748390022504 34.0 29.0 35.0 2.0 35.0 94-95 28.286627494720037 33.5 29.0 35.0 2.0 35.0 96-97 27.647791046498487 33.0 27.5 35.0 2.0 35.0 98-99 26.849772707656697 33.0 26.5 35.0 2.0 35.0 100-101 23.572023514612468 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3397173453032494 1101 2 0.2034668079104449 1101 3 0.28064100882020426 1101 4 0.18016835150250188 1101 5 0.19823821730643942 1101 6 0.267830710796197 1101 7 0.22992994449253246 1101 8 0.2521444398215209 1101 9 0.2595647685338136 1101 10-11 0.2550229014469636 1101 12-13 0.30402867924330224 1101 14-15 0.3456918181067081 1101 16-17 0.3165229828286087 1101 18-19 0.3053298482392819 1101 20-21 0.2566050301164964 1101 22-23 0.31869976322325044 1101 24-25 0.39517708825150066 1101 26-27 0.2906835488685786 1101 28-29 0.3911705574528881 1101 30-31 0.2411591644829585 1101 32-33 0.4350977174067552 1101 34-35 0.5128415034054683 1101 36-37 0.5371637023088525 1101 38-39 0.4278643947673473 1101 40-41 0.5334309991727153 1101 42-43 0.4445341679799526 1101 44-45 0.40602788875873586 1101 46-47 0.5071684500667075 1101 48-49 0.5783440216472187 1101 50-51 0.6077581789219195 1101 52-53 0.6048423027196108 1101 54-55 0.5084468526392811 1101 56-57 0.5173604143022743 1101 58-59 0.7122884637790676 1101 60-61 0.5819659311369492 1101 62-63 0.5669910826656732 1101 64-65 0.6717766970550585 1101 66-67 0.6340204792767352 1101 68-69 0.44821602934132443 1101 70-71 0.5144025978195472 1101 72-73 0.6131114176748298 1101 74-75 0.5335512315050615 1101 76-77 0.4707637191534424 1101 78-79 0.5985011451239224 1101 80-81 0.9632793269124917 1101 82-83 0.8922413244237895 1101 84-85 0.8071835750724716 1101 86-87 0.7300863425592503 1101 88-89 0.4956294755042201 1101 90-91 0.5267165769622792 1101 92-93 0.756937676836845 1101 94-95 0.696896536668266 1101 96-97 0.7946677631009571 1101 98-99 0.930127884634949 1101 100-101 1.190474542705843 1102 1 0.30072823728995246 1102 2 0.2752174708354147 1102 3 0.17907920319112236 1102 4 0.16353706917259103 1102 5 0.2618334153415134 1102 6 0.2775753793800888 1102 7 0.2887903782089225 1102 8 0.2725214124628721 1102 9 0.3300452278011221 1102 10-11 0.26653443607092697 1102 12-13 0.3573176256505164 1102 14-15 0.3658289127679879 1102 16-17 0.39432701440168927 1102 18-19 0.43798526946230965 1102 20-21 0.35101638520391987 1102 22-23 0.4038125860586277 1102 24-25 0.36283915997801586 1102 26-27 0.35310505476515885 1102 28-29 0.2810294670794704 1102 30-31 0.47737183220433366 1102 32-33 0.4539939772810655 1102 34-35 0.49193956220040747 1102 36-37 0.42324504094197835 1102 38-39 0.3654026032816944 1102 40-41 0.4517637051972869 1102 42-43 0.3558644271138718 1102 44-45 0.3976652960439111 1102 46-47 0.4147572696467847 1102 48-49 0.4258056817458211 1102 50-51 0.3194783677792188 1102 52-53 0.28135018169204074 1102 54-55 0.2386274055005444 1102 56-57 0.2134453506220595 1102 58-59 0.3231948837369103 1102 60-61 0.1523272725832996 1102 62-63 0.1786387767842541 1102 64-65 0.10594134976429359 1102 66-67 0.13940599594086223 1102 68-69 0.12182595431931986 1102 70-71 0.10646908044128622 1102 72-73 0.12116167631410235 1102 74-75 0.17227335329776494 1102 76-77 0.1774005815687829 1102 78-79 0.09664510978235441 1102 80-81 0.18670378360770457 1102 82-83 0.2111748943568692 1102 84-85 0.059799819767722084 1102 86-87 0.14918486120886953 1102 88-89 0.09068907112578728 1102 90-91 0.11362331611338305 1102 92-93 0.1143264982717902 1102 94-95 0.26155867486117046 1102 96-97 0.152184590304568 1102 98-99 0.40834478070083335 1102 100-101 0.6852139403503017 1103 1 0.24666458973661776 1103 2 0.20819014150963255 1103 3 0.22235616861960494 1103 4 0.15592714665079654 1103 5 0.1293901194789555 1103 6 0.22068227070120372 1103 7 0.18493664194125614 1103 8 0.17680237693117107 1103 9 0.19284573036244979 1103 10-11 0.2530644901575627 1103 12-13 0.3083632238976861 1103 14-15 0.3868717530566457 1103 16-17 0.33931914911263306 1103 18-19 0.3797342492730351 1103 20-21 0.37029719645230585 1103 22-23 0.38710586940821656 1103 24-25 0.39840341785734523 1103 26-27 0.2730035976437506 1103 28-29 0.30930045693120434 1103 30-31 0.3438738725828898 1103 32-33 0.38122536999903645 1103 34-35 0.34613165911710553 1103 36-37 0.4536751547843778 1103 38-39 0.31580276263040474 1103 40-41 0.40996914246138516 1103 42-43 0.3108018112413049 1103 44-45 0.43446744316567987 1103 46-47 0.5607675507031828 1103 48-49 0.6559962617853543 1103 50-51 0.6139593863852184 1103 52-53 0.5107650859040618 1103 54-55 0.4987648182290343 1103 56-57 0.5823695425146838 1103 58-59 0.7163616585087453 1103 60-61 0.6750121196442933 1103 62-63 0.6603162284305952 1103 64-65 0.48206815456723007 1103 66-67 0.6344863597870969 1103 68-69 0.6531013313679921 1103 70-71 0.6920379349181118 1103 72-73 0.6790207088835558 1103 74-75 0.6686382107777042 1103 76-77 0.7298005161949526 1103 78-79 0.6797260558642613 1103 80-81 1.0054471759988033 1103 82-83 0.8377805113990071 1103 84-85 0.767478563568698 1103 86-87 0.7619144995587952 1103 88-89 0.563938788945773 1103 90-91 0.5535023745259195 1103 92-93 0.5650599109389951 1103 94-95 0.6852730320780829 1103 96-97 1.1162666497845422 1103 98-99 1.1420543114903765 1103 100-101 1.2963411537396823 1104 1 0.10037290125735154 1104 2 0.07154386385486333 1104 3 0.06644767996635181 1104 4 0.10293804893836267 1104 5 -0.003051841190810478 1104 6 0.04179598916407201 1104 7 0.04984674745841744 1104 8 0.13762642885479437 1104 9 0.17334648555686982 1104 10-11 -0.0013997219929464677 1104 12-13 0.12191494824167393 1104 14-15 0.19863395364301795 1104 16-17 0.18175631297144434 1104 18-19 0.17467239141690527 1104 20-21 0.12217911631387324 1104 22-23 0.1583376430204737 1104 24-25 0.14239287247858812 1104 26-27 0.33000370937015333 1104 28-29 0.2825825458129074 1104 30-31 0.2805125086588518 1104 32-33 0.2736163674948813 1104 34-35 0.25626696395363524 1104 36-37 0.3545971963752095 1104 38-39 0.38571623714221204 1104 40-41 0.40891770105501024 1104 42-43 0.4780082474063292 1104 44-45 0.5160564171279987 1104 46-47 0.4794970060378816 1104 48-49 0.4423289359268878 1104 50-51 0.5719720797357226 1104 52-53 0.45081653799416443 1104 54-55 0.3671230503733156 1104 56-57 0.4480577490777833 1104 58-59 0.39152547711794483 1104 60-61 0.38587496797217113 1104 62-63 0.3246107160455409 1104 64-65 0.41104067548604917 1104 66-67 0.2603336560534544 1104 68-69 0.48857687206854905 1104 70-71 0.46983721092379227 1104 72-73 0.4840508052960075 1104 74-75 0.5509725646179398 1104 76-77 0.5149229417117418 1104 78-79 0.645510615130771 1104 80-81 0.7825000399701914 1104 82-83 0.7729160923826335 1104 84-85 0.59308279606174 1104 86-87 0.5459298015487377 1104 88-89 0.5201176844623951 1104 90-91 0.5498528868648052 1104 92-93 0.5547580848732778 1104 94-95 0.4773909427255738 1104 96-97 0.6387298131193049 1104 98-99 0.7920508075910888 1104 100-101 1.0579854621487321 1105 1 0.16778378847286746 1105 2 0.14842965891968873 1105 3 0.10332988519932229 1105 4 0.09596293929921984 1105 5 0.15546835198194486 1105 6 0.19506909391528637 1105 7 0.16564167591793932 1105 8 0.20925020857917076 1105 9 0.12941564178790088 1105 10-11 0.16767647495245086 1105 12-13 0.1592885439595264 1105 14-15 0.1905997698930193 1105 16-17 0.29037756691371897 1105 18-19 0.3174069992756827 1105 20-21 0.3077044093329775 1105 22-23 0.03693802507588373 1105 24-25 0.22213440827057696 1105 26-27 0.3249743512534664 1105 28-29 0.28619836156533296 1105 30-31 0.21674111755478265 1105 32-33 0.026167857072387335 1105 34-35 0.0975264605147288 1105 36-37 0.14729030371375984 1105 38-39 0.23951250275880653 1105 40-41 0.3855127529103797 1105 42-43 0.2769517716850487 1105 44-45 0.27490679006140084 1105 46-47 0.3476904692268121 1105 48-49 0.35594105127768927 1105 50-51 0.16804136127915825 1105 52-53 0.26819674209858846 1105 54-55 0.19903064795389724 1105 56-57 0.0980348611354529 1105 58-59 0.25014074186435664 1105 60-61 0.1526467434904788 1105 62-63 0.20624061764949886 1105 64-65 0.16575647616414102 1105 66-67 0.2059428199285449 1105 68-69 0.11863143087857964 1105 70-71 0.09356828460416722 1105 72-73 0.06997300693178232 1105 74-75 0.09909391051740002 1105 76-77 0.06912166286178589 1105 78-79 0.14056317078514624 1105 80-81 0.4369688646393364 1105 82-83 0.2890255549803804 1105 84-85 0.022051946727426497 1105 86-87 0.12795257597277043 1105 88-89 -0.03263616622197674 1105 90-91 0.1977531300814448 1105 92-93 0.25493193740785003 1105 94-95 0.3708800971211481 1105 96-97 0.5634921526134598 1105 98-99 0.57708723480172 1105 100-101 0.7958709774720418 1106 1 0.17396176328285406 1106 2 0.1360920983186773 1106 3 0.12387262606053184 1106 4 0.17632552661947898 1106 5 0.07670928711505098 1106 6 0.1699802197866518 1106 7 0.10650075203891163 1106 8 0.0850092153367683 1106 9 0.18989933183141972 1106 10-11 0.20844695278007208 1106 12-13 0.09187936548527631 1106 14-15 0.226157512515897 1106 16-17 0.20030925538608102 1106 18-19 0.16610472573093915 1106 20-21 0.11094374535699103 1106 22-23 0.24731465425102073 1106 24-25 0.12501445964214497 1106 26-27 0.16850384392985518 1106 28-29 0.2858182605434223 1106 30-31 0.13424338124301727 1106 32-33 0.18121909991262442 1106 34-35 0.1720681877732062 1106 36-37 0.28034846376736766 1106 38-39 0.2682081990663647 1106 40-41 0.3742937963327435 1106 42-43 0.369094732331817 1106 44-45 0.37899470575436567 1106 46-47 0.47387481399296405 1106 48-49 0.4669265040925765 1106 50-51 0.3325276419676726 1106 52-53 0.33717376832941426 1106 54-55 0.36993432847926755 1106 56-57 0.1652408409501973 1106 58-59 0.24742541932464235 1106 60-61 0.2573790104078171 1106 62-63 0.24829041952620656 1106 64-65 0.36565062897100375 1106 66-67 0.31913661003039095 1106 68-69 0.5145446185267488 1106 70-71 0.5090613640807717 1106 72-73 0.5355846220246079 1106 74-75 0.4852783876844917 1106 76-77 0.33076742856950503 1106 78-79 0.41096413231016626 1106 80-81 0.7232540768522959 1106 82-83 0.5884076103872324 1106 84-85 0.39988155701998096 1106 86-87 0.4959994120685032 1106 88-89 0.32272187901059723 1106 90-91 0.3981299946360224 1106 92-93 0.5787116237206114 1106 94-95 0.5671174096175129 1106 96-97 0.7337389773815595 1106 98-99 0.9858390838175772 1106 100-101 0.9428108240487809 1107 1 0.08289061953784227 1107 2 0.03499482911647078 1107 3 -0.007402021245795254 1107 4 0.11741784573506209 1107 5 0.0449555814251994 1107 6 0.034869255344773364 1107 7 0.07878741705555115 1107 8 0.10038253436984945 1107 9 0.043597871555867584 1107 10-11 0.057154705165736175 1107 12-13 0.1386818845293547 1107 14-15 0.04215014238692305 1107 16-17 0.12077538854341441 1107 18-19 0.10526128597074091 1107 20-21 0.18000542893645388 1107 22-23 0.06871690379038853 1107 24-25 0.07317275241226895 1107 26-27 0.05878297381462971 1107 28-29 0.07497344575916287 1107 30-31 0.1366293229970026 1107 32-33 0.11587218134155108 1107 34-35 0.09923343816509345 1107 36-37 0.08074777075524509 1107 38-39 0.07223509453703514 1107 40-41 0.27736009999305367 1107 42-43 0.06524934003745386 1107 44-45 0.10369068793405489 1107 46-47 0.25145697168073156 1107 48-49 0.08114232786949316 1107 50-51 0.14507198649370423 1107 52-53 0.09479030161875102 1107 54-55 0.23327151187998396 1107 56-57 0.14219241260270366 1107 58-59 0.01633750028606329 1107 60-61 0.034066302435427076 1107 62-63 0.1217942558433478 1107 64-65 0.23768676460337446 1107 66-67 0.1532603071950973 1107 68-69 0.11006286509606866 1107 70-71 -0.08317937513216123 1107 72-73 -0.11477700930394263 1107 74-75 0.03178087647804162 1107 76-77 0.12915617537256807 1107 78-79 0.1438277574236082 1107 80-81 0.3546167256986479 1107 82-83 0.2800309637959124 1107 84-85 0.11768509358187274 1107 86-87 0.19342976709971182 1107 88-89 0.1198552288442194 1107 90-91 0.06985894989273689 1107 92-93 0.1974144260196482 1107 94-95 -0.07069272705987828 1107 96-97 -0.20641117524611374 1107 98-99 -0.023995363285248317 1107 100-101 0.22815279253322984 1108 1 0.043023221858376814 1108 2 0.04743054674152347 1108 3 0.060825272731847235 1108 4 0.07468249786647618 1108 5 0.037119010951883524 1108 6 0.019214248048811555 1108 7 0.08504579324380046 1108 8 0.09530397882846131 1108 9 0.02768899315049822 1108 10-11 0.07723715660863917 1108 12-13 0.06545140299592589 1108 14-15 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-1.281637617167263 2316 56-57 -1.408065641924864 2316 58-59 -1.2846433766098997 2316 60-61 -1.1128351953496036 2316 62-63 -1.1627637252682845 2316 64-65 -0.9424484805409321 2316 66-67 -1.0768633012732067 2316 68-69 -1.260101496709666 2316 70-71 -0.9529899420330601 2316 72-73 -0.9049307832327926 2316 74-75 -0.9016716204130155 2316 76-77 -0.9704072245345365 2316 78-79 -0.8003085065541384 2316 80-81 -0.8650788228982087 2316 82-83 -1.116949493580389 2316 84-85 -1.2401248140738623 2316 86-87 -1.2466202756013907 2316 88-89 -1.2296709636653596 2316 90-91 -1.3821417093524246 2316 92-93 -1.3158559793661873 2316 94-95 -1.1602475867455375 2316 96-97 -1.329138452513643 2316 98-99 -1.3170928666439004 2316 100-101 -1.6842306284497468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 8.0 5 49.0 6 90.0 7 194.0 8 525.0 9 984.0 10 1721.0 11 2765.0 12 4286.0 13 6057.0 14 7673.0 15 10492.0 16 13919.0 17 19109.0 18 17191.0 19 11214.0 20 9660.0 21 9822.0 22 10408.0 23 11514.0 24 13227.0 25 15839.0 26 19635.0 27 24761.0 28 31174.0 29 38708.0 30 48813.0 31 61559.0 32 78490.0 33 103354.0 34 140212.0 35 201252.0 36 309332.0 37 476306.0 38 368300.0 39 28425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4655894217134 19.716854732196815 15.254223544624642 22.56333230146514 2 15.33810032037434 20.990814155223706 40.05232431153853 23.618761212863426 3 19.246358481900785 24.981269603726336 30.28129216127551 25.491079753097374 4 12.925801007783692 17.565830916079857 37.75478916606305 31.753578910073404 5 13.54729181966447 37.54656696773455 34.44300851701018 14.463132695590803 6 31.103072416442107 37.04281049974751 17.666668733032946 14.187448350777437 7 27.73152561253057 33.09250629089319 21.200393499720324 17.97557459685591 8 25.850219138353047 35.073008764103555 19.983720197207827 19.09305190033556 9 25.878710339476957 17.0930136632907 20.73584256959323 36.29243342763911 10-11 24.367441569917013 27.08970429821671 27.816548637414236 20.726305494452042 12-13 25.65187225063481 24.391736108627494 28.251087824708225 21.70530381602947 14-15 22.436565336832803 25.72316064392424 26.87826734233665 24.962006676906306 16-17 21.651192361182474 29.00742628375619 27.32811384981535 22.013267505245988 18-19 21.42749504762577 27.528166777461237 29.901300636059386 21.143037538853605 20-21 22.227745919160256 26.57216137810437 30.05414097253467 21.145951730200704 22-23 21.880744597604572 26.598854883387794 29.95206666034041 21.56833385866722 24-25 21.930080154739468 26.616042202366874 30.04422149566226 21.4096561472314 26-27 21.796628638806062 26.79861368597627 29.979338818342548 21.42541885687512 28-29 21.45323067854598 26.96445552553191 30.10437958362623 21.477934212295875 30-31 21.71172183214402 26.676531316327306 30.014293724713788 21.597453126814887 32-33 21.644109319583944 26.786755282687093 29.94541173665544 21.623723661073527 34-35 22.111321282433458 26.73302124759742 29.714232440050903 21.441425029918218 36-37 22.00146133495619 26.53460440222386 29.869467113524834 21.59446714929511 38-39 21.74918981456643 26.887879301625045 29.84515449379595 21.517776390012575 40-41 22.034678723329677 26.779187997004115 29.710190371249812 21.4759429084164 42-43 21.803464475758947 26.89204587858812 29.435287446956192 21.869202198696737 44-45 21.999612301718916 26.900395161353778 29.20197816729348 21.89801436963383 46-47 22.13134844791345 27.19277288836498 28.854275152500087 21.82160351122149 48-49 22.261801555896987 27.260361440382557 28.632786757041067 21.84505024667939 50-51 21.99693183953244 27.363422987455543 28.616676410643283 22.02296876236873 52-53 22.31831872215101 27.626293261307456 28.218775750778246 21.836612265763293 54-55 22.095560588005 27.38552170971032 28.372976986319397 22.145940715965278 56-57 22.20124832443274 27.579419861510285 28.27999891275892 21.939332901298062 58-59 22.469675832485514 27.34374050004089 28.23971405525433 21.94686961221927 60-61 22.303790726002866 27.330903444959258 28.728299870510334 21.637005958527542 62-63 22.109981257231798 27.392814421798356 28.602665950749028 21.894538370220815 64-65 22.051513405078566 27.47205948942504 28.398255433410245 22.078171672086146 66-67 22.041953199993706 27.62844108226266 28.147503327291517 22.182102390452116 68-69 22.048613470573578 27.370988566094557 28.415216118814385 22.165181844517484 70-71 22.105696356759896 27.39990853630913 28.358155218365987 22.136239888564987 72-73 22.20523324995171 27.315604604191655 28.343845458642637 22.135316687213997 74-75 22.050405067975497 27.702853502164114 28.00033572857871 22.24640570128168 76-77 22.145010747586113 27.46324778580651 28.094988870913323 22.29675259569405 78-79 22.147084794173285 27.58263745964433 27.998075184642303 22.27220256154008 80-81 22.257027281306314 27.651798193390132 27.86747935340476 22.22369517189879 82-83 22.13417906899611 27.565301343402552 28.108446952635767 22.19207263496557 84-85 22.10201174261616 27.551396298393588 27.91948260960272 22.427109349387536 86-87 22.125871271420866 27.50177747237944 28.00807694893707 22.364274307262626 88-89 22.119459012500393 27.45942641438895 27.820099053243265 22.601015519867396 90-91 21.964528615895958 27.4295823761321 28.10415144185204 22.501737566119907 92-93 22.09140250520248 27.310574342560784 28.088013977557324 22.51000917467941 94-95 21.943584580093592 27.26684893698036 28.052183321622543 22.737383161303505 96-97 21.867073773778976 27.368503623860498 28.117380749263376 22.64704185309715 98-99 21.81869465357828 27.387902819903243 27.790749546213284 23.00265298030519 100-101 21.469758224109302 27.39868463844247 28.030734368875372 23.100822768572854 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 434.0 1 301.5 2 231.5 3 306.0 4 271.0 5 270.5 6 461.5 7 1035.0 8 1787.5 9 2186.0 10 2111.5 11 1938.0 12 2068.5 13 2313.0 14 2533.0 15 2744.5 16 2959.0 17 3164.0 18 3309.0 19 3569.0 20 4038.5 21 4610.5 22 5495.5 23 6677.5 24 8233.5 25 10171.5 26 12734.0 27 16069.0 28 19164.0 29 22323.0 30 26186.0 31 30395.5 32 34757.0 33 39247.5 34 44568.5 35 48775.5 36 53237.0 37 58460.5 38 61852.5 39 63484.5 40 65032.5 41 68718.5 42 72106.5 43 74276.0 44 77317.0 45 79461.0 46 78944.0 47 80593.5 48 87381.0 49 87131.0 50 79533.5 51 74067.5 52 69629.0 53 66506.0 54 63112.0 55 57860.5 56 52305.5 57 46894.5 58 41761.5 59 36862.0 60 31582.0 61 25582.0 62 19600.0 63 14835.5 64 11143.0 65 8481.5 66 6346.5 67 4854.5 68 3442.0 69 2183.0 70 1522.0 71 1061.5 72 769.0 73 593.0 74 473.0 75 360.5 76 218.0 77 102.5 78 63.0 79 47.5 80 27.0 81 15.0 82 8.0 83 7.0 84 5.5 85 1.0 86 1.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026608439643904688 2 0.007486603985829814 3 0.010490782655302924 4 9.537075141184476E-5 5 0.004720852194886316 6 0.0 7 0.0 8 9.537075141184476E-5 9 0.0 10-11 0.0 12-13 1.9074150282368953E-4 14-15 0.0 16-17 2.384268785296119E-5 18-19 0.00500696444912185 20-21 0.0025511676002668475 22-23 0.0 24-25 0.006437525720299521 26-27 0.007224334419447241 28-29 0.010156985025361467 30-31 0.033594347184822314 32-33 7.868086991477193E-4 34-35 0.005364604766916268 36-37 0.01745284750836759 38-39 0.008702581066330834 40-41 0.009656288580449283 42-43 0.005221548639798501 44-45 0.004172470374268209 46-47 0.001478246646883594 48-49 0.0011921343926480595 50-51 0.0027180664152375755 52-53 0.002622695663825731 54-55 0.0010490782655302924 56-57 0.0013351905197658265 58-59 0.0014544039590306326 60-61 2.384268785296119E-5 62-63 1.668988149707283E-4 64-65 0.00803498580644792 66-67 0.0013351905197658265 68-69 0.0012636624562069431 70-71 0.0034333470508264114 72-73 0.01401950045754118 74-75 0.00700975022877059 76-77 0.00460163875562151 78-79 0.012135928117157246 80-81 9.537075141184476E-5 82-83 0.006079885402505103 84-85 7.868086991477193E-4 86-87 0.0 88-89 0.0011921343926480595 90-91 0.001740516213266167 92-93 1.4305612711776715E-4 94-95 5.00696444912185E-4 96-97 2.861122542355343E-4 98-99 1.668988149707283E-4 100-101 6.199098841769909E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2097079.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.51729383046693 #Duplication Level Percentage of deduplicated Percentage of total 1 78.00841704903662 45.648414617080824 2 12.981315047042974 15.192628538273508 3 4.000749716215514 7.023391400578214 4 1.7315935407589644 4.053126720781238 5 0.8782565879606604 2.5696599408118646 6 0.5287494066653318 1.8564590635512166 7 0.3466621046217411 1.4200009765226944 8 0.2593087586456973 1.2139237457987122 9 0.18335548732741236 0.965652023462998 >10 0.9572392094730251 10.34408116755043 >50 0.08362030942498581 3.3731794526455223 >100 0.03795908133555987 4.022609642695434 >500 0.0018763274795748283 0.7241390835460906 >1k 7.342151007031916E-4 0.8389014513254281 >5k 1.6315891126737593E-4 0.7538321753758513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8821 0.4206326991019413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6970 0.332367068670279 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4638 0.22116477252406802 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3547 0.16914002762890668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.768537570592238E-5 0.0 3 0.0 0.0 0.0 4.768537570592238E-5 0.0 4 0.0 0.0 0.0 4.768537570592238E-5 0.0 5 0.0 0.0 0.0 4.768537570592238E-5 0.0 6 0.0 0.0 0.0 4.768537570592238E-5 0.0 7 0.0 0.0 0.0 4.768537570592238E-5 0.0 8 0.0 0.0 0.0 4.768537570592238E-5 0.0 9 0.0 0.0 0.0 4.768537570592238E-5 0.0 10-11 0.0 0.0 0.0 9.537075141184476E-5 0.0 12-13 0.0 0.0 0.0 9.537075141184476E-5 0.0 14-15 0.0 0.0 0.0 1.1921343926480596E-4 0.0 16-17 0.0 0.0 0.0 2.384268785296119E-4 0.0 18-19 0.0 0.0 0.0 2.384268785296119E-4 0.0 20-21 0.0 0.0 0.0 2.622695663825731E-4 2.384268785296119E-5 22-23 0.0 0.0 0.0 5.00696444912185E-4 4.768537570592238E-5 24-25 0.0 0.0 0.0 9.298648262654865E-4 4.768537570592238E-5 26-27 0.0 0.0 0.0 0.0013351905197658267 4.768537570592238E-5 28-29 0.0 0.0 0.0 0.0021696845946194685 4.768537570592238E-5 30-31 0.0 0.0 0.0 0.0038386727443267517 4.768537570592238E-5 32-33 0.0 0.0 0.0 0.005698402396857725 1.4305612711776715E-4 34-35 0.0 0.0 0.0 0.007748873552212387 1.4305612711776715E-4 36-37 0.0 0.0 0.0 0.010466939967449962 1.4305612711776715E-4 38-39 0.0 0.0 0.0 0.015068578723071473 1.4305612711776715E-4 40-41 0.0 0.0 0.0 0.023079721841666432 1.4305612711776715E-4 42-43 0.0 0.0 0.0 0.030614011203202167 1.4305612711776715E-4 44-45 0.0 0.0 0.0 0.04010340096868072 1.4305612711776715E-4 46-47 0.0 0.0 0.0 0.057007866656430206 1.4305612711776715E-4 48-49 0.0 0.0 0.0 0.06790397500523347 1.4305612711776715E-4 50-51 0.0 0.0 0.0 0.08051675687944995 1.4305612711776715E-4 52-53 0.0 0.0 0.0 0.09436935852202039 1.4305612711776715E-4 54-55 0.0 0.0 0.0 0.1120606329089176 1.4305612711776715E-4 56-57 0.0 0.0 0.0 0.16387079361340226 1.6689881497072834E-4 58-59 0.0 0.0 0.0 0.21193765232497203 1.9074150282368953E-4 60-61 0.0 0.0 0.0 0.24517435919199992 1.9074150282368953E-4 62-63 0.0 0.0 0.0 0.2891641182807133 1.9074150282368953E-4 64-65 0.0 0.0 0.0 0.33777935881290116 1.9074150282368953E-4 66-67 0.0 0.0 0.0 0.4101657591344914 1.9074150282368953E-4 68-69 0.0 0.0 0.0 0.5017693658655682 1.9074150282368953E-4 70-71 0.0 0.0 0.0 0.5461644506477821 2.1458419067665072E-4 72-73 0.0 0.0 0.0 0.594732005804264 4.768537570592238E-4 74-75 0.0 0.0 0.0 0.6898643303375791 4.768537570592238E-4 76-77 0.0 0.0 0.0 0.8048337711645579 4.768537570592238E-4 78-79 0.0 0.0 0.0 0.9122450799421481 4.768537570592238E-4 80-81 0.0 0.0 0.0 0.9898530289035368 4.768537570592238E-4 82-83 0.0 0.0 0.0 1.1054662223025455 5.245391327651462E-4 84-85 0.0 0.0 0.0 1.330755779825176 5.245391327651462E-4 86-87 0.0 0.0 0.0 1.5692303437304935 5.722245084710686E-4 88-89 0.0 0.0 0.0 1.8481897916101397 6.199098841769909E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12285 0.0 28.776268 1 GGTATCA 8695 0.0 27.323547 1 ATCAACG 14900 0.0 23.400341 3 TCAACGC 14965 0.0 23.327105 4 TATCAAC 15070 0.0 23.295639 2 CAACGCA 15140 0.0 23.057472 5 AACGCAG 15390 0.0 22.712154 6 GTACATG 7765 0.0 22.45749 1 GAGTACT 8660 0.0 22.101505 12-13 TACATGG 7815 0.0 21.518784 2 AGTACTT 8915 0.0 21.309504 12-13 AGAGTAC 12855 0.0 21.242956 10-11 ACATGGG 7765 0.0 20.799353 3 GTACTTT 9080 0.0 20.76684 14-15 ACGCAGA 17055 0.0 20.43918 7 CGCAGAG 17065 0.0 20.315884 8 GCAGAGT 18015 0.0 18.796389 9 CATGGGG 3600 0.0 18.338446 4 ATCACGC 130 0.0075632706 18.27002 3 ACTTTTT 10845 0.0 17.562895 16-17 >>END_MODULE