FastQCFastQC Report
Thu 26 May 2016
SRR936869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162237
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.3643911375117529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.2636158755955059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.17098033194326062No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32220.14901234231030178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110550.031.1230871
GGTATCA75500.026.1218531
TCAACGC132300.025.8627954
ATCAACG132200.025.846413
GTACATG79400.025.5570181
CAACGCA133850.025.4567895
TATCAAC135700.025.2492332
AACGCAG136400.024.9431446
ACATGGG77700.023.8531883
TACATGG81550.023.7754632
ACGCAGA151550.022.2918517
CGCAGAG152900.021.939658
GAGTACT68750.021.87097512-13
AGAGTAC112450.021.50505410-11
AGTACTT73750.021.38667312-13
GCAGAGT158150.020.5484549
CATGGGG39700.019.3922124
GTGTAGC10800.019.3611761
GTACTTT77200.019.35197614-15
CATGGGA45650.017.3851534
Produced by FastQC (version 0.11.5)