FastQCFastQC Report
Thu 26 May 2016
SRR936869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162237
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88480.4092058363629889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.31980768065665327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.2146388208138146No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36920.1707490899471242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122600.028.1737211
GGTATCA86600.027.7059971
ATCAACG146600.023.3582823
TCAACGC147250.023.2229184
CAACGCA148000.023.1416055
TATCAAC148950.023.023242
AACGCAG151550.022.6308676
GTACATG81150.021.6041981
GAGTACT86800.021.42257712-13
TACATGG80700.020.7764282
AGAGTAC129500.020.4317410-11
AGTACTT90650.020.22456612-13
ACATGGG78800.020.1307453
ACGCAGA169850.020.0527327
GTACTTT93400.020.01048314-15
CGCAGAG170900.019.8183468
GCAGAGT182800.018.268349
ACTTTTT110050.017.02694316-17
TACTTTT107950.016.80739414-15
GTGTAGC12650.016.5257991
Produced by FastQC (version 0.11.5)