##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162237 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19490185395958 33.0 31.0 34.0 31.0 34.0 2 32.32186295951831 34.0 31.0 34.0 31.0 34.0 3 32.43504712943123 34.0 31.0 34.0 31.0 34.0 4 36.07453715758263 37.0 35.0 37.0 35.0 37.0 5 35.982280388320056 37.0 35.0 37.0 35.0 37.0 6 36.01980217709715 37.0 35.0 37.0 35.0 37.0 7 35.969113931544044 37.0 35.0 37.0 35.0 37.0 8 35.9340733693855 37.0 35.0 37.0 35.0 37.0 9 37.622335571910014 39.0 37.0 39.0 35.0 39.0 10-11 37.53318946997947 39.0 37.0 39.0 35.0 39.0 12-13 37.44068272811907 39.0 37.0 39.0 34.5 39.0 14-15 38.653757659313015 40.0 38.0 41.0 34.0 41.0 16-17 38.56675817683261 40.0 38.0 41.0 34.0 41.0 18-19 38.723641302965405 40.0 38.0 41.0 34.0 41.0 20-21 38.748960451606365 40.0 38.0 41.0 34.0 41.0 22-23 38.677100613855 40.0 38.0 41.0 34.0 41.0 24-25 38.569879250054456 40.0 38.0 41.0 34.0 41.0 26-27 38.40169763999043 40.0 38.0 41.0 34.0 41.0 28-29 38.3249576711526 40.0 38.0 41.0 34.0 41.0 30-31 38.323014544659074 40.0 38.0 41.0 34.0 41.0 32-33 38.170513454353056 40.0 38.0 41.0 34.0 41.0 34-35 38.02065684751487 40.0 37.0 41.0 33.0 41.0 36-37 37.867064526229086 40.0 37.0 41.0 33.0 41.0 38-39 37.724885847388606 40.0 37.0 41.0 33.0 41.0 40-41 37.55476457946099 40.0 37.0 41.0 33.0 41.0 42-43 37.31355559080711 40.0 37.0 41.0 32.0 41.0 44-45 37.063334639079805 40.0 36.0 41.0 31.0 41.0 46-47 36.735786132602485 39.5 36.0 41.0 31.0 41.0 48-49 36.39928462976076 39.0 36.0 41.0 30.0 41.0 50-51 36.11526419166816 39.0 35.0 40.0 29.5 41.0 52-53 35.88631310998748 39.0 35.0 40.0 29.0 41.0 54-55 35.609410763019966 38.5 35.0 40.0 28.0 41.0 56-57 35.32731680199719 38.0 34.5 40.0 28.0 41.0 58-59 34.97847992611356 38.0 34.0 40.0 27.0 41.0 60-61 34.646146329010186 38.0 34.0 40.0 26.0 41.0 62-63 34.28413675281664 37.0 34.0 40.0 26.0 41.0 64-65 33.90497017671976 37.0 33.0 39.0 26.0 40.5 66-67 33.506380197915405 36.0 33.0 39.0 25.0 40.0 68-69 33.196326998381764 36.0 32.0 39.0 24.0 40.0 70-71 32.71513483489552 35.0 32.0 38.0 22.5 40.0 72-73 32.20508968258336 35.0 31.0 37.5 22.0 39.5 74-75 31.68557933288534 35.0 31.0 37.0 20.5 39.0 76-77 30.273940830723 33.5 29.5 35.5 20.0 38.0 78-79 31.02100024187913 34.0 31.0 36.0 20.0 38.0 80-81 30.789576027049762 34.0 31.0 36.0 18.0 37.5 82-83 30.448137970074512 34.0 30.0 35.0 17.5 37.0 84-85 30.071182298702688 34.0 30.0 35.0 14.5 36.5 86-87 29.699587973011283 34.0 30.0 35.0 9.0 36.0 88-89 29.29541165006426 34.0 29.0 35.0 6.5 36.0 90-91 28.823033275260762 34.0 29.0 35.0 2.0 35.0 92-93 28.320622808693035 33.0 29.0 35.0 2.0 35.0 94-95 27.715945569333982 33.0 27.0 35.0 2.0 35.0 96-97 27.016787475193517 33.0 26.0 34.0 2.0 35.0 98-99 26.14204802711266 32.0 25.0 34.0 2.0 35.0 100-101 22.475884234706925 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21153230188961203 1101 2 0.1844693109076303 1101 3 0.21621395637261998 1101 4 0.1850262413824595 1101 5 0.23957928516892935 1101 6 0.21696414299813682 1101 7 0.18824984824418323 1101 8 0.1917283167226813 1101 9 0.26900323060319664 1101 10-11 0.31661342757364963 1101 12-13 0.2779980741283481 1101 14-15 0.37010698280439414 1101 16-17 0.44603360599419517 1101 18-19 0.3512814622261118 1101 20-21 0.37357304528067203 1101 22-23 0.42001237095428223 1101 24-25 0.3818850753281211 1101 26-27 0.3030218276639616 1101 28-29 0.3961085362469774 1101 30-31 0.46738291325841885 1101 32-33 0.4712328034915245 1101 34-35 0.44360195882968867 1101 36-37 0.36317677564910156 1101 38-39 0.40642885471704915 1101 40-41 0.570015946535186 1101 42-43 0.5386436499571445 1101 44-45 0.5955947582816137 1101 46-47 0.56682437910478 1101 48-49 0.5444064929042725 1101 50-51 0.41700603995811747 1101 52-53 0.39428001391242873 1101 54-55 0.39322623598141604 1101 56-57 0.5069434505718959 1101 58-59 0.4907419101376007 1101 60-61 0.4994488491316318 1101 62-63 0.479505001910546 1101 64-65 0.48563985771284024 1101 66-67 0.319667314652726 1101 68-69 0.24380253731186485 1101 70-71 0.23569429777862183 1101 72-73 0.4706249437835126 1101 74-75 0.48100955436258275 1101 76-77 0.38154294894315655 1101 78-79 0.48656041605843825 1101 80-81 0.6455801869889548 1101 82-83 0.590804381434868 1101 84-85 0.4000147290705023 1101 86-87 -0.04053275457178174 1101 88-89 -0.13837325593532768 1101 90-91 0.3829386634093268 1101 92-93 0.5167752918559891 1101 94-95 0.5828980941802193 1101 96-97 0.5167599140829786 1101 98-99 0.662508549303837 1101 100-101 0.9531808458380127 1102 1 0.3070637559711713 1102 2 0.3177088042310956 1102 3 0.31044834369138385 1102 4 0.2506303562853134 1102 5 0.30600368532869027 1102 6 0.22126771783911892 1102 7 0.26905846797766486 1102 8 0.29490124131489637 1102 9 0.34043054182971133 1102 10-11 0.34980554033639777 1102 12-13 0.3463453300626682 1102 14-15 0.4446505915196113 1102 16-17 0.4084951929857752 1102 18-19 0.42578659682069997 1102 20-21 0.43820680459975137 1102 22-23 0.4291650666616178 1102 24-25 0.46094599218979226 1102 26-27 0.4231534431667825 1102 28-29 0.4150574657842796 1102 30-31 0.41345154896397673 1102 32-33 0.5001743761817821 1102 34-35 0.5713217975104641 1102 36-37 0.4861723749324227 1102 38-39 0.5204248045187327 1102 40-41 0.4188843928885504 1102 42-43 0.5069997485622793 1102 44-45 0.5104081662363242 1102 46-47 0.3252219936242753 1102 48-49 0.3953706097287082 1102 50-51 0.4345960465497285 1102 52-53 0.4284306849457309 1102 54-55 0.4244439461505536 1102 56-57 0.45973857417903474 1102 58-59 0.5252593607020728 1102 60-61 0.45598425244350693 1102 62-63 0.5514831732674779 1102 64-65 0.474129081026625 1102 66-67 0.4650500694465265 1102 68-69 0.24060535914416192 1102 70-71 0.15484295264046466 1102 72-73 0.3707250827792521 1102 74-75 0.3589453051319076 1102 76-77 0.3141100379252961 1102 78-79 0.37497983328816886 1102 80-81 0.7609431371760245 1102 82-83 0.5389881372360072 1102 84-85 0.5750910024204146 1102 86-87 0.4890695739415172 1102 88-89 0.6243018943103635 1102 90-91 0.7796264029824691 1102 92-93 0.8377184349705757 1102 94-95 0.6463987800186182 1102 96-97 0.9303650008293651 1102 98-99 0.8031597312352012 1102 100-101 1.001971876308879 1103 1 0.305736633205143 1103 2 0.2998132074002129 1103 3 0.28507919524177794 1103 4 0.1849191585269523 1103 5 0.21755741207640256 1103 6 0.2246382838001466 1103 7 0.1530843866348519 1103 8 0.15004313405342629 1103 9 0.2704086196702491 1103 10-11 0.28213333539233787 1103 12-13 0.28216618129107474 1103 14-15 0.361349973967215 1103 16-17 0.35542046561620566 1103 18-19 0.3352473217843013 1103 20-21 0.2926823234326008 1103 22-23 0.35487819633209483 1103 24-25 0.22727103756839995 1103 26-27 0.3507231287500261 1103 28-29 0.36437716504219964 1103 30-31 0.3335726076983718 1103 32-33 0.36336473896617605 1103 34-35 0.4355591945963795 1103 36-37 0.3040798619453611 1103 38-39 0.26874320691275244 1103 40-41 0.350534951882274 1103 42-43 0.44363991673949243 1103 44-45 0.48585959552154634 1103 46-47 0.33312867373415855 1103 48-49 0.43689207587155465 1103 50-51 0.47627326825837457 1103 52-53 0.35153408452917034 1103 54-55 0.5263272889171517 1103 56-57 0.5182198429720231 1103 58-59 0.47534038075167473 1103 60-61 0.3729201777460105 1103 62-63 0.4451742775880163 1103 64-65 0.3485737047828579 1103 66-67 0.3318783818126647 1103 68-69 0.29390615514626717 1103 70-71 0.4097804816627928 1103 72-73 0.46565596648938623 1103 74-75 0.6282423141207367 1103 76-77 0.46344665111265115 1103 78-79 0.6673664895944071 1103 80-81 0.6332873883990828 1103 82-83 0.6391058946506938 1103 84-85 0.6344859531469531 1103 86-87 0.3762723824296721 1103 88-89 0.34208774456700297 1103 90-91 0.38148462701211727 1103 92-93 0.25988322785059026 1103 94-95 0.010575077474122452 1103 96-97 0.2714263382634208 1103 98-99 0.33742692554405096 1103 100-101 0.3691960754866912 1104 1 0.20333202281794627 1104 2 0.20950749633978205 1104 3 0.16418253022320073 1104 4 0.1015673063797422 1104 5 0.1305009110866635 1104 6 0.09736902024641125 1104 7 0.15974938749434386 1104 8 0.1926909877317584 1104 9 0.27228761472776597 1104 10-11 0.27933017177640096 1104 12-13 0.16688443836056877 1104 14-15 0.14545914813444227 1104 16-17 0.22540589866405014 1104 18-19 0.19864732284758446 1104 20-21 0.20462447739217282 1104 22-23 0.1258463670099914 1104 24-25 0.23823444120233717 1104 26-27 0.19497768889717548 1104 28-29 0.09607658962986676 1104 30-31 0.21231549035997688 1104 32-33 0.12509848981458305 1104 34-35 0.15794463047964058 1104 36-37 0.16187016871970172 1104 38-39 0.13545949336779017 1104 40-41 0.2282897805079287 1104 42-43 0.24035925126724322 1104 44-45 0.24984050253583945 1104 46-47 0.36091114767047827 1104 48-49 0.30217762889296296 1104 50-51 0.32961126230753734 1104 52-53 0.3980669018166978 1104 54-55 0.38690099999885064 1104 56-57 0.2629763604426216 1104 58-59 0.1958625935815803 1104 60-61 0.3273458198349317 1104 62-63 0.3456881968247316 1104 64-65 0.2885637985465408 1104 66-67 0.15759853329479512 1104 68-69 0.24526033233499334 1104 70-71 0.03427777118184849 1104 72-73 0.2168285884167247 1104 74-75 0.2193737516237242 1104 76-77 0.18048201052827295 1104 78-79 0.31701525237815176 1104 80-81 0.5727069432032543 1104 82-83 0.5519759386528129 1104 84-85 0.4745222996419365 1104 86-87 0.219336953723559 1104 88-89 0.0539837344020313 1104 90-91 0.2091957725026532 1104 92-93 0.12069006333086207 1104 94-95 0.13384498608510498 1104 96-97 0.18396073983991457 1104 98-99 0.088760900390362 1104 100-101 0.2263535527457634 1105 1 0.27219355630310105 1105 2 0.28656121675295054 1105 3 0.24833456922682018 1105 4 0.1623747342500863 1105 5 0.15882792728687178 1105 6 0.1965395758845716 1105 7 0.22982304535354103 1105 8 0.1711783017284887 1105 9 0.21643807118736902 1105 10-11 0.24958843078668735 1105 12-13 0.20176466499478352 1105 14-15 0.2973672875409221 1105 16-17 0.27787427604341275 1105 18-19 0.18308082860865227 1105 20-21 0.23431369878921515 1105 22-23 0.21424336295885382 1105 24-25 0.1674715941186946 1105 26-27 0.3278918219717397 1105 28-29 0.24532066400248453 1105 30-31 0.33527707366918236 1105 32-33 0.25402104689268157 1105 34-35 0.3678654424116701 1105 36-37 0.3348455340743328 1105 38-39 0.3502299331846004 1105 40-41 0.3908880917073887 1105 42-43 0.5045101077105087 1105 44-45 0.5097250116037273 1105 46-47 0.5599018845707917 1105 48-49 0.4484132175254274 1105 50-51 0.44286280767916253 1105 52-53 0.41975505890940923 1105 54-55 0.5610382309120183 1105 56-57 0.44301613273018603 1105 58-59 0.413508568050446 1105 60-61 0.3319507620677271 1105 62-63 0.5559419516712651 1105 64-65 0.4869949061447514 1105 66-67 0.3603316389852509 1105 68-69 0.324109544584573 1105 70-71 0.08907214045545686 1105 72-73 0.201114100226242 1105 74-75 0.3349196380092394 1105 76-77 0.2576534652601232 1105 78-79 0.4683565453861753 1105 80-81 0.46272491638174174 1105 82-83 0.5239708223401713 1105 84-85 0.562183586528473 1105 86-87 0.4941183322237457 1105 88-89 0.5380478639550006 1105 90-91 0.47270248135890114 1105 92-93 0.4113511406607486 1105 94-95 0.22924265244134645 1105 96-97 0.23517264683153982 1105 98-99 0.20084718903769527 1105 100-101 0.29614113534021413 1106 1 0.1156842240758067 1106 2 0.13459631902542668 1106 3 0.10010308633044929 1106 4 0.07743666531166582 1106 5 0.09430715625934027 1106 6 0.0784702008709317 1106 7 0.14087528399659988 1106 8 0.12115529314939266 1106 9 0.1370087887319542 1106 10-11 0.05468358676040452 1106 12-13 0.18158950290873577 1106 14-15 0.22010683653784469 1106 16-17 0.20696035083220465 1106 18-19 0.007931665780390063 1106 20-21 0.15321059677868476 1106 22-23 0.13925119984680379 1106 24-25 0.15041407267094087 1106 26-27 0.07657186301171492 1106 28-29 0.19338388302058007 1106 30-31 0.12566603653673525 1106 32-33 0.0991013586218159 1106 34-35 0.017941540994918626 1106 36-37 0.15196837019824017 1106 38-39 0.18318232245308508 1106 40-41 0.22807528199750493 1106 42-43 0.16783006667053968 1106 44-45 0.030410291788840027 1106 46-47 0.011928691530123103 1106 48-49 -0.10784245275770843 1106 50-51 -0.1340890576748066 1106 52-53 -0.12084648227245509 1106 54-55 -0.3248147674070907 1106 56-57 -0.15060677979791848 1106 58-59 -0.1843713245470724 1106 60-61 -0.1927031705656006 1106 62-63 0.008842560721888049 1106 64-65 -0.2065645254080053 1106 66-67 -0.05619734003633425 1106 68-69 -0.23221423458545587 1106 70-71 -0.29558607081307287 1106 72-73 -0.3405175442104351 1106 74-75 -0.10604829405651373 1106 76-77 -0.1175358643475981 1106 78-79 -0.10903881360441758 1106 80-81 -0.04336323223534677 1106 82-83 0.0756877338641111 1106 84-85 0.04683786843814275 1106 86-87 -0.13841169462687475 1106 88-89 -0.06970511035136084 1106 90-91 -0.08614263641271691 1106 92-93 -0.01146926580959473 1106 94-95 0.11661360574746737 1106 96-97 0.1473499045756519 1106 98-99 -0.1303872845373668 1106 100-101 -0.045299542255889946 1107 1 0.08249138775760656 1107 2 0.06242814348453152 1107 3 0.08950609823338596 1107 4 0.16249876662909912 1107 5 0.041927928306080275 1107 6 0.03331883849998718 1107 7 0.008196531722333589 1107 8 0.08075247853267342 1107 9 0.08947192909013779 1107 10-11 0.040161918157402 1107 12-13 0.0802625119619762 1107 14-15 0.11840324812073533 1107 16-17 0.17404941459950152 1107 18-19 0.16991741733127697 1107 20-21 0.12881137715528013 1107 22-23 0.16289827672266455 1107 24-25 0.18880395408000794 1107 26-27 0.1842497165376571 1107 28-29 0.1839284368249139 1107 30-31 0.21217718320808387 1107 32-33 0.18157041756655445 1107 34-35 0.05574210654874179 1107 36-37 0.08816206142338956 1107 38-39 0.1963313173894079 1107 40-41 0.19351889102006226 1107 42-43 0.25560744013334613 1107 44-45 0.13048806640029653 1107 46-47 0.08537317738031902 1107 48-49 0.24939808298397992 1107 50-51 0.1133518770234545 1107 52-53 0.0825195713455571 1107 54-55 0.24155319331102731 1107 56-57 0.21583597431769164 1107 58-59 0.1338837884540851 1107 60-61 0.21426217282202487 1107 62-63 0.2879678374706103 1107 64-65 0.17443952991982314 1107 66-67 0.33607596713830645 1107 68-69 0.2936165815218672 1107 70-71 0.0941205865007646 1107 72-73 0.18055841098367154 1107 74-75 0.18842988389565463 1107 76-77 0.1799688595613027 1107 78-79 0.4096872435209029 1107 80-81 0.2870620730102793 1107 82-83 0.4169468583517144 1107 84-85 0.4437438067533215 1107 86-87 0.357548058984559 1107 88-89 0.33150043996758427 1107 90-91 0.4807017924436785 1107 92-93 0.4301050782137281 1107 94-95 0.45603542560322907 1107 96-97 0.4696838443814322 1107 98-99 0.2904531547532798 1107 100-101 0.46941134575129695 1108 1 0.037551902120476655 1108 2 -0.004524048264478608 1108 3 -0.003548786715001029 1108 4 0.046374585332955576 1108 5 0.049695095133074574 1108 6 0.06927730713216107 1108 7 0.07816860579667662 1108 8 0.009514953361865253 1108 9 0.014999517766931092 1108 10-11 0.028406123189974153 1108 12-13 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2316 64-65 -1.14025413404498 2316 66-67 -1.235625140306233 2316 68-69 -1.5219659214950383 2316 70-71 -1.288444370408218 2316 72-73 -1.030131579298157 2316 74-75 -1.0951980916273634 2316 76-77 -0.9697598859400394 2316 78-79 -1.0523235639418012 2316 80-81 -1.2157545952582858 2316 82-83 -1.1921395265947297 2316 84-85 -1.1388868279181317 2316 86-87 -1.1079231656120285 2316 88-89 -1.3950438417953563 2316 90-91 -1.263060315225129 2316 92-93 -1.385571829912159 2316 94-95 -1.4865226965467322 2316 96-97 -1.0304530643852097 2316 98-99 -1.100468256197285 2316 100-101 -1.4629594612842318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 8.0 5 46.0 6 95.0 7 209.0 8 523.0 9 1014.0 10 1809.0 11 2989.0 12 4510.0 13 6230.0 14 8073.0 15 10814.0 16 14557.0 17 19817.0 18 18203.0 19 11833.0 20 10421.0 21 10758.0 22 11420.0 23 13014.0 24 14970.0 25 18038.0 26 21742.0 27 27547.0 28 34091.0 29 42825.0 30 53382.0 31 67210.0 32 85805.0 33 111424.0 34 152638.0 35 215757.0 36 330595.0 37 486673.0 38 333122.0 39 20070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.450322884480954 19.71697934270259 15.221254167613107 22.611443605203345 2 15.374305024635463 21.00635161579315 39.960372355555435 23.658971004015953 3 19.279004621611083 24.905086547102 30.304735891090367 25.51117294019655 4 12.951457298517266 17.58086596183552 37.71817853872409 31.749498200923128 5 13.586629766593472 37.468157962405584 34.41640967960944 14.528802591391504 6 31.139740925717206 37.053893722103545 17.603944433473295 14.202420918705952 7 27.731557641461137 33.08517983921281 21.213446999565726 17.96981551976032 8 25.837038215514767 35.01928789489774 19.978198504604258 19.16547538498324 9 25.87815304242782 17.146362771518568 20.735007309559496 36.24047687649411 10-11 24.359367219962593 27.051062138078297 27.82558238765467 20.76398825430444 12-13 25.660323082067322 24.361390541369886 28.276225039160828 21.70206133740196 14-15 22.445180616185922 25.665734144776913 26.864793267343035 25.02429197169413 16-17 21.68293762432148 29.04168229477157 27.294972752755598 21.980407328151355 18-19 21.48715018456644 27.557343010998604 29.812954637793997 21.14255216664096 20-21 22.30475852638974 26.506979999893627 29.970860879371997 21.21740059434464 22-23 21.939810576357633 26.582579102072646 29.827459698856323 21.6501506227134 24-25 21.973364847472876 26.628584511740904 29.955908727712977 21.442141913073243 26-27 21.870567117569003 26.805470954517098 29.857190363668067 21.466771564245835 28-29 21.509881351632657 26.97969850101481 30.041815635204312 21.468604512148225 30-31 21.73526758532305 26.63407455301468 29.96765532186389 21.663002539798384 32-33 21.73160361367045 26.79401218154564 29.81847008703945 21.65591411774446 34-35 22.15973549615514 26.746281744323124 29.65868218886274 21.435300570659 36-37 22.10335708760249 26.528519576747723 29.71070514680971 21.65741818884008 38-39 21.805881875430313 26.900711927885357 29.762484871850436 21.530921324833894 40-41 22.010918893144556 26.76328978309516 29.692993135975577 21.532798187784703 42-43 21.86235828912372 26.89705152580406 29.342481883345812 21.898108301726406 44-45 22.028806277942707 26.888588068745474 29.137902089363934 21.944703563947893 46-47 22.186685375733354 27.15282799274731 28.773006035662696 21.887480595856644 48-49 22.264068129844823 27.213573614045732 28.617675196463914 21.904683059645528 50-51 22.08273225403465 27.316643255601196 28.566687772856085 22.03393671750807 52-53 22.38558955378157 27.551928858862375 28.174941044853085 21.887540542502972 54-55 22.1427704247223 27.333255479718943 28.32188846660369 22.20208562895507 56-57 22.19690307285417 27.685102342524925 28.15932379217136 21.95867079244955 58-59 22.546689720064016 27.301140745430697 28.182200153406683 21.969969381098604 60-61 22.3162366796365 27.292795244836615 28.668086210460935 21.72288186506595 62-63 22.154118036925187 27.371846770439408 28.539284320982773 21.934750871652632 64-65 22.035879541307953 27.460081813493908 28.377697151327215 22.126341493870925 66-67 22.080698847971984 27.57465011120685 28.112647055966946 22.232003984854224 68-69 22.043866850119667 27.409751529096187 28.364994392350475 22.181387228433675 70-71 22.161870044435894 27.33297690887497 28.3188173614477 22.18633568524144 72-73 22.25475283236759 27.248423738933337 28.271368957241965 22.225454471457105 74-75 22.060149770885324 27.64219539402729 27.986168022362033 22.311486812725345 76-77 22.203902717417193 27.45644903865765 27.968465066502883 22.37118317742227 78-79 22.187948836121905 27.518587061864835 27.993545431609952 22.29991867040331 80-81 22.236359162772455 27.67014740651119 27.837709464134413 22.255783966581937 82-83 22.227379488787687 27.477338091894627 28.047320117779943 22.247962301537743 84-85 22.101164471646978 27.561945160006445 27.866655473604148 22.47023489474243 86-87 22.183816289162063 27.452677605535637 27.96700671604066 22.396499389261642 88-89 22.153386443588488 27.43785760220402 27.79272247213122 22.616033482076272 90-91 22.02364517529924 27.40548242845191 28.062123274398452 22.508749121850396 92-93 22.128317091224588 27.283505289332144 28.08857709137721 22.499600528066054 94-95 21.988649712612315 27.265085772320873 27.973634700374983 22.77262981469183 96-97 21.93946311741913 27.341472935260892 28.055218191219446 22.66384575610054 98-99 21.80188567548918 27.42464142936163 27.730996634902667 23.042476260246527 100-101 21.57145133231633 27.364103873291956 27.927526690609533 23.136918103782182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 439.0 1 320.0 2 242.0 3 288.0 4 257.5 5 252.5 6 454.5 7 1049.5 8 1755.5 9 2143.0 10 2044.0 11 1922.0 12 2055.0 13 2254.0 14 2499.0 15 2761.5 16 2995.0 17 3172.0 18 3369.0 19 3627.0 20 3987.0 21 4725.5 22 5684.5 23 6838.5 24 8347.0 25 10217.0 26 12977.5 27 16380.5 28 19421.5 29 22646.0 30 26832.0 31 31081.5 32 35523.5 33 40479.0 34 45775.0 35 49986.0 36 54937.0 37 60402.0 38 63572.5 39 65008.5 40 66800.0 41 70521.0 42 73737.0 43 76506.0 44 79791.5 45 81709.5 46 81285.0 47 83226.5 48 90224.0 49 89814.5 50 82021.0 51 76605.0 52 72098.5 53 69054.0 54 65885.5 55 60161.0 56 54496.0 57 49022.5 58 43330.5 59 38557.5 60 33198.5 61 26590.0 62 20415.5 63 15487.5 64 11617.5 65 8771.5 66 6508.0 67 5004.0 68 3596.5 69 2276.5 70 1541.5 71 1083.0 72 833.5 73 633.5 74 460.5 75 379.0 76 229.5 77 103.5 78 68.0 79 40.5 80 20.0 81 10.5 82 5.5 83 4.5 84 5.0 85 1.5 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029043994714732936 2 0.005087323915000992 3 1.84993596909127E-4 4 7.862227868637896E-4 5 4.624839922728175E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011723969204115923 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.468629942046131E-4 20-21 0.001086837381841121 22-23 0.002150550564068601 24-25 0.011307733611070387 26-27 0.005919795101092064 28-29 3.468629942046131E-4 30-31 0.0026592829555687003 32-33 0.005272317511910119 34-35 0.0 36-37 0.007839103669024256 38-39 0.013435159975525347 40-41 9.943405833865576E-4 42-43 0.0 44-45 0.0 46-47 2.543661957500496E-4 48-49 0.003306760544750645 50-51 0.007122253481001389 52-53 0.0 54-55 0.0027517797540232637 56-57 0.002844276552477827 58-59 4.8560819188645833E-4 60-61 0.00184993596909127 62-63 0.005017951316160069 64-65 9.24967984545635E-5 66-67 0.0023124199613640873 68-69 5.549807907273809E-4 70-71 9.712163837729167E-4 72-73 0.0 74-75 4.624839922728175E-5 76-77 0.0 78-79 0.002196798963295883 80-81 0.0024511651590459326 82-83 0.011053367415320337 84-85 0.0084865812582062 86-87 0.004624839922728175 88-89 2.543661957500496E-4 90-91 9.24967984545635E-4 92-93 3.468629942046131E-4 94-95 4.624839922728175E-5 96-97 0.0023586683605913692 98-99 0.0018961843683185516 100-101 3.0061459497733134E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162237.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.2779720259057 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74344542105824 45.30730337445959 2 13.188703300821428 15.372217640464827 3 4.016447360084696 7.022112206836162 4 1.7659837839093295 4.1167181422748405 5 0.8854019206268134 2.5799714180986304 6 0.5595074368935616 1.9564175253341514 7 0.34419535840747734 1.4041305228102288 8 0.23817921492330657 1.1104481299562114 9 0.17283602498202702 0.9065279726074179 >10 0.9579763716724032 10.377867009905048 >50 0.0846097242864571 3.4009809038171976 >100 0.039767802213702366 4.095708866664437 >500 0.0019907298112620754 0.7523849933326094 >1k 7.962919245048119E-4 0.8657091254314174 >5k 1.5925838490096238E-4 0.73150216800726 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8848 0.4092058363629889 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6915 0.31980768065665327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4641 0.2146388208138146 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3692 0.1707490899471242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.624839922728175E-5 0.0 3 0.0 0.0 0.0 4.624839922728175E-5 0.0 4 0.0 0.0 0.0 4.624839922728175E-5 0.0 5 0.0 0.0 0.0 4.624839922728175E-5 0.0 6 0.0 0.0 0.0 4.624839922728175E-5 0.0 7 0.0 0.0 0.0 4.624839922728175E-5 0.0 8 0.0 0.0 0.0 4.624839922728175E-5 0.0 9 0.0 0.0 0.0 4.624839922728175E-5 0.0 10-11 0.0 0.0 0.0 4.624839922728175E-5 0.0 12-13 0.0 0.0 0.0 4.624839922728175E-5 0.0 14-15 0.0 0.0 0.0 4.624839922728175E-5 0.0 16-17 0.0 0.0 0.0 9.24967984545635E-5 0.0 18-19 0.0 0.0 0.0 1.3874519768184525E-4 0.0 20-21 0.0 0.0 0.0 2.774903953636905E-4 0.0 22-23 0.0 0.0 0.0 5.087323915000992E-4 0.0 24-25 0.0 0.0 0.0 5.781049903410218E-4 0.0 26-27 0.0 0.0 0.0 9.480921841592758E-4 0.0 28-29 0.0 0.0 0.0 0.0017111907714094246 0.0 30-31 0.0 0.0 0.0 0.0034223815428188493 0.0 32-33 0.0 0.0 0.0 0.005272317511910119 0.0 34-35 0.0 0.0 0.0 0.007561613273660566 0.0 36-37 0.0 0.0 0.0 0.010660256021888442 0.0 38-39 0.0 0.0 0.0 0.015308220144230257 0.0 40-41 0.0 0.0 0.0 0.022522970423686207 0.0 42-43 0.0 0.0 0.0 0.029968962699278574 2.3124199613640874E-5 44-45 0.0 0.0 0.0 0.03859428915516662 4.624839922728175E-5 46-47 0.0 0.0 0.0 0.05565994847003358 1.1562099806820437E-4 48-49 0.0 0.0 0.0 0.06669019168574028 1.3874519768184525E-4 50-51 0.0 0.0 0.0 0.07896914168058358 1.3874519768184525E-4 52-53 0.0 0.0 0.0 0.09291303404760903 2.0811779652276787E-4 54-55 0.0 0.0 0.0 0.11004806596131692 2.774903953636905E-4 56-57 0.0 0.0 0.0 0.15786891076232623 2.774903953636905E-4 58-59 0.0 0.0 0.0 0.20272985801278953 2.774903953636905E-4 60-61 0.0 0.0 0.0 0.23394752749120473 2.774903953636905E-4 62-63 0.0 0.0 0.0 0.27834599074939514 3.2373879459097224E-4 64-65 0.0 0.0 0.0 0.32507999816856337 3.2373879459097224E-4 66-67 0.0 0.0 0.0 0.39766686075578206 3.2373879459097224E-4 68-69 0.0 0.0 0.0 0.48551569508800374 3.2373879459097224E-4 70-71 0.0 0.0 0.0 0.5293360533558533 3.2373879459097224E-4 72-73 0.0 0.0 0.0 0.5767637867634307 3.2373879459097224E-4 74-75 0.0 0.0 0.0 0.6646588694948796 3.2373879459097224E-4 76-77 0.0 0.0 0.0 0.7757706486384239 3.2373879459097224E-4 78-79 0.0 0.0 0.0 0.8828125686499676 3.2373879459097224E-4 80-81 0.0 0.0 0.0 0.9649959740768472 3.2373879459097224E-4 82-83 0.0 0.0 0.0 1.0778189439918011 3.2373879459097224E-4 84-85 0.0 0.0 0.0 1.2988400438989807 3.2373879459097224E-4 86-87 0.0 0.0 0.0 1.5234916431455017 3.2373879459097224E-4 88-89 0.0 0.0 0.0 1.7918479796618039 3.2373879459097224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12260 0.0 28.173721 1 GGTATCA 8660 0.0 27.705997 1 ATCAACG 14660 0.0 23.358282 3 TCAACGC 14725 0.0 23.222918 4 CAACGCA 14800 0.0 23.141605 5 TATCAAC 14895 0.0 23.02324 2 AACGCAG 15155 0.0 22.630867 6 GTACATG 8115 0.0 21.604198 1 GAGTACT 8680 0.0 21.422577 12-13 TACATGG 8070 0.0 20.776428 2 AGAGTAC 12950 0.0 20.43174 10-11 AGTACTT 9065 0.0 20.224566 12-13 ACATGGG 7880 0.0 20.130745 3 ACGCAGA 16985 0.0 20.052732 7 GTACTTT 9340 0.0 20.010483 14-15 CGCAGAG 17090 0.0 19.818346 8 GCAGAGT 18280 0.0 18.26834 9 ACTTTTT 11005 0.0 17.026943 16-17 TACTTTT 10795 0.0 16.807394 14-15 GTGTAGC 1265 0.0 16.525799 1 >>END_MODULE