FastQCFastQC Report
Thu 26 May 2016
SRR936868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166527
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.3660697512655047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55860.2578320048630827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.17530360803257933No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33220.15333296100163996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115500.028.1499651
GGTATCA82200.024.6922021
GTACATG82550.024.1844371
ATCAACG134100.023.967463
TCAACGC134800.023.9135424
TATCAAC136850.023.6242582
CAACGCA136050.023.5540435
TACATGG83350.023.0446362
AACGCAG140150.022.8278966
ACATGGG83350.022.0754453
GAGTACT66200.021.42493612-13
ACGCAGA154800.020.3864067
CGCAGAG155900.020.1811268
AGAGTAC111500.020.157410-11
GTACTTT72650.019.4909914-15
AGTACTT70500.019.41049412-13
CATGGGG40150.019.1837524
GTGTAGC12350.018.8596171
GCAGAGT162350.018.7038889
TACGCTG1851.5111474E-417.989975
Produced by FastQC (version 0.11.5)