##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936868_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2166527 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.627640458669568 33.0 31.0 34.0 30.0 34.0 2 31.706250141355266 33.0 31.0 34.0 30.0 34.0 3 31.729580106779192 33.0 31.0 34.0 30.0 34.0 4 35.321295326575665 37.0 35.0 37.0 33.0 37.0 5 35.33066885388458 37.0 35.0 37.0 33.0 37.0 6 35.389340635957915 37.0 35.0 37.0 33.0 37.0 7 35.332008324844324 37.0 35.0 37.0 33.0 37.0 8 35.294234043702204 37.0 35.0 37.0 33.0 37.0 9 36.89406870996761 39.0 37.0 39.0 33.0 39.0 10-11 36.76208904850944 39.0 37.0 39.0 32.5 39.0 12-13 36.66967778384483 39.0 37.0 39.0 32.0 39.0 14-15 37.7915701027497 40.0 37.5 41.0 32.0 41.0 16-17 37.72309299630238 40.0 37.0 41.0 32.0 41.0 18-19 37.676155662957356 40.0 37.0 41.0 32.0 41.0 20-21 37.606532021064126 40.0 37.0 41.0 32.0 41.0 22-23 37.489911965094365 40.0 37.0 41.0 32.0 41.0 24-25 37.4416196982544 40.0 37.0 41.0 32.0 41.0 26-27 37.46815202395355 40.0 37.0 41.0 32.0 41.0 28-29 37.399403053827626 40.0 37.0 41.0 32.0 41.0 30-31 37.24189797773118 40.0 37.0 41.0 31.0 41.0 32-33 37.06382426805666 39.5 36.0 41.0 31.0 41.0 34-35 36.898029657604084 39.0 36.0 41.0 30.0 41.0 36-37 36.71838430815771 39.0 36.0 41.0 30.0 41.0 38-39 36.56051020827343 39.0 35.5 41.0 30.0 41.0 40-41 36.38886337442368 39.0 35.0 41.0 30.0 41.0 42-43 36.17392744239975 39.0 35.0 40.5 30.0 41.0 44-45 35.94214011641674 39.0 35.0 40.0 28.5 41.0 46-47 35.621019724194525 39.0 35.0 40.0 27.5 41.0 48-49 35.407492729146696 38.5 35.0 40.0 27.0 41.0 50-51 34.71757679456568 38.0 34.0 39.5 25.0 40.5 52-53 34.90731548695216 38.0 34.5 40.0 26.0 40.5 54-55 35.30400521202829 39.0 35.0 40.0 26.5 41.0 56-57 35.19105116160564 39.0 35.0 40.5 26.0 41.0 58-59 34.98159658291819 38.0 34.5 40.0 26.0 41.0 60-61 34.69757288969858 38.0 34.0 40.0 25.5 41.0 62-63 34.3961328430248 38.0 34.0 40.0 25.0 41.0 64-65 34.0744500299327 37.0 34.0 40.0 23.5 41.0 66-67 33.70744652616838 37.0 33.0 39.5 23.0 41.0 68-69 33.31536855991179 36.0 33.0 39.0 22.0 41.0 70-71 32.90114201207739 36.0 32.5 39.0 21.5 40.5 72-73 32.50602900402349 35.0 32.0 38.5 20.5 40.0 74-75 32.10719644850953 35.0 32.0 37.5 20.0 39.5 76-77 31.649964436169036 35.0 31.0 37.0 18.0 39.0 78-79 31.181899648608116 35.0 31.0 36.5 16.5 39.0 80-81 30.732931092019626 35.0 31.0 36.0 12.0 38.5 82-83 30.326045786643785 34.0 30.0 35.5 7.5 37.0 84-85 29.946694640777615 34.0 30.0 35.0 7.0 37.0 86-87 29.599039384231077 34.0 30.0 35.0 2.0 36.0 88-89 29.232523527285835 34.0 29.0 35.0 2.0 36.0 90-91 28.862381359659953 34.0 29.0 35.0 2.0 36.0 92-93 28.48200691706127 34.0 29.0 35.0 2.0 35.0 94-95 28.105082466085122 34.0 29.0 35.0 2.0 35.0 96-97 27.601437000323557 34.0 27.0 35.0 2.0 35.0 98-99 27.026663872640405 34.0 26.5 35.0 2.0 35.0 100-101 24.85509481303487 31.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22150341387173 1101 2 0.34426266769072456 1101 3 0.30965596589973643 1101 4 0.35528685383019365 1101 5 0.33379122117801074 1101 6 0.1340572651370806 1101 7 0.12756503542101427 1101 8 0.2584593303594289 1101 9 0.31669254878216435 1101 10-11 0.3186686460382262 1101 12-13 0.3750116061583242 1101 14-15 0.47873256044555745 1101 16-17 0.5555484412385212 1101 18-19 0.5574781943783478 1101 20-21 0.572971764869358 1101 22-23 0.5175390940297504 1101 24-25 0.6282172931379009 1101 26-27 0.4870221347561241 1101 28-29 0.5308783049527435 1101 30-31 0.4985751505290068 1101 32-33 0.46558383179336715 1101 34-35 0.5062842261505551 1101 36-37 0.5739325841153899 1101 38-39 0.6115511418341697 1101 40-41 0.7119408676091368 1101 42-43 0.5963418738770301 1101 44-45 0.7305827330229633 1101 46-47 0.6389999083898701 1101 48-49 0.7434055223894731 1101 50-51 0.6955158803830059 1101 52-53 0.6318841987497166 1101 54-55 0.8083186359589902 1101 56-57 0.7607279360782755 1101 58-59 0.8299178189073615 1101 60-61 0.8644964944219922 1101 62-63 0.7617722925065777 1101 64-65 0.7750465260450028 1101 66-67 0.7090088362391569 1101 68-69 0.6976418509864928 1101 70-71 0.8243498762064974 1101 72-73 0.7675417783988152 1101 74-75 0.7960740254973402 1101 76-77 0.866538876746386 1101 78-79 0.9023401887837785 1101 80-81 0.9402013921686567 1101 82-83 0.6742877303308852 1101 84-85 0.7181524202281331 1101 86-87 0.8100190367844 1101 88-89 0.8644779588368863 1101 90-91 0.9055425912885298 1101 92-93 0.9050842923083984 1101 94-95 0.9577139254290543 1101 96-97 1.0093029957633313 1101 98-99 0.8025182160219835 1101 100-101 0.9393802594983072 1102 1 0.21924759866472598 1102 2 0.2042276808965262 1102 3 0.2543995675794939 1102 4 0.13042349343191262 1102 5 0.22542251720842899 1102 6 0.08568238702734732 1102 7 0.1987961883681848 1102 8 0.06766526733419909 1102 9 0.27172854009916136 1102 10-11 0.3051162123979836 1102 12-13 0.2382274511580107 1102 14-15 0.16643533929864418 1102 16-17 0.3564628019945104 1102 18-19 0.2432792400620869 1102 20-21 0.2065774137175893 1102 22-23 0.032091444368099076 1102 24-25 0.06732106279346795 1102 26-27 0.08351806521375238 1102 28-29 -0.14432331012391586 1102 30-31 0.04894035581916967 1102 32-33 0.11938401505752694 1102 34-35 0.035716254257593505 1102 36-37 0.09367709231572263 1102 38-39 0.16716270021655077 1102 40-41 0.04218406012960685 1102 42-43 0.04870446250779281 1102 44-45 -0.03794012844598882 1102 46-47 0.06918939569123239 1102 48-49 0.25979090633769175 1102 50-51 0.2640378598319373 1102 52-53 0.27769739670930704 1102 54-55 -0.04109996584327291 1102 56-57 0.20838778995696572 1102 58-59 -0.17126879202870526 1102 60-61 -0.02494748995645324 1102 62-63 -0.1650566005092884 1102 64-65 0.11688249972160492 1102 66-67 -0.049223987645945044 1102 68-69 0.159723222233616 1102 70-71 0.07404023817771588 1102 72-73 0.047395461077414325 1102 74-75 0.12622676196446037 1102 76-77 0.002411905064132469 1102 78-79 -0.0519601978349975 1102 80-81 0.19485527281174697 1102 82-83 0.2029728834838629 1102 84-85 0.09038270585561747 1102 86-87 0.20539685986098277 1102 88-89 0.264694935762261 1102 90-91 0.13304867967673673 1102 92-93 0.35390929463595455 1102 94-95 0.37884496910374565 1102 96-97 0.19632701120847784 1102 98-99 0.37882774624832294 1102 100-101 0.3380280248025791 1103 1 0.29321011190891255 1103 2 0.2707869319811387 1103 3 0.17730734561573058 1103 4 0.2605172696438913 1103 5 0.2338119701799286 1103 6 0.27862686229150313 1103 7 0.2686465771459581 1103 8 0.32364380195460285 1103 9 0.35512062662797206 1103 10-11 0.3800579955014456 1103 12-13 0.1646292316944198 1103 14-15 0.5182517208520139 1103 16-17 0.46639674195083813 1103 18-19 0.37124450874425463 1103 20-21 0.3571839048880747 1103 22-23 0.45570938405263206 1103 24-25 0.5569753171659855 1103 26-27 0.5882253916083897 1103 28-29 0.5355962018931919 1103 30-31 0.45099907857444066 1103 32-33 0.41859549384400196 1103 34-35 0.4677980696252604 1103 36-37 0.41424770958200696 1103 38-39 0.5124226925441775 1103 40-41 0.5818718586518656 1103 42-43 0.47331650266441727 1103 44-45 0.44524977638281626 1103 46-47 0.525019156794329 1103 48-49 0.5699228394694202 1103 50-51 0.5022135470465798 1103 52-53 0.645743142588465 1103 54-55 0.7158577487538977 1103 56-57 0.6052450123215962 1103 58-59 0.6715301275117156 1103 60-61 0.6452660600823918 1103 62-63 0.7153060142661758 1103 64-65 0.8063878008118124 1103 66-67 0.696781004787745 1103 68-69 0.6863885742188884 1103 70-71 0.6311429512271047 1103 72-73 0.4359023293589175 1103 74-75 0.46109139352350326 1103 76-77 0.5459247433414269 1103 78-79 0.6373350478480369 1103 80-81 0.5662330529224775 1103 82-83 0.4547135480175548 1103 84-85 0.37708829284408196 1103 86-87 0.5676500380163709 1103 88-89 0.5218672897742032 1103 90-91 0.5117993416613622 1103 92-93 0.5127221481213624 1103 94-95 0.6086241026292356 1103 96-97 0.7915732515133023 1103 98-99 0.8164665473020314 1103 100-101 0.8017230441255201 1104 1 0.2849249885859315 1104 2 0.17602639054027946 1104 3 0.17385150045951647 1104 4 0.22287082007097325 1104 5 0.30579030624357273 1104 6 0.09799764502159292 1104 7 0.23029611471688582 1104 8 0.24156422572547598 1104 9 0.3626452242651865 1104 10-11 0.3209244262299009 1104 12-13 0.21586436239832096 1104 14-15 0.04545126011833389 1104 16-17 0.38077869195856096 1104 18-19 0.3700323085490993 1104 20-21 0.4106089933649031 1104 22-23 0.2989430515879903 1104 24-25 0.22714700656735687 1104 26-27 0.28916942986449357 1104 28-29 0.3043625713480438 1104 30-31 0.4493419682843438 1104 32-33 0.13492335182623094 1104 34-35 0.26928399741693454 1104 36-37 0.2316215344148489 1104 38-39 0.3327573615211321 1104 40-41 0.31737520472513125 1104 42-43 0.3307480767590718 1104 44-45 0.2381185251648006 1104 46-47 0.11364651496339206 1104 48-49 0.11887930438300742 1104 50-51 0.18162260094988625 1104 52-53 0.21480190599870497 1104 54-55 0.3512542684540989 1104 56-57 0.3333598196762111 1104 58-59 0.3662872096823975 1104 60-61 0.3603352232232879 1104 62-63 0.17057508212300831 1104 64-65 0.08586814650592345 1104 66-67 0.07629376829594037 1104 68-69 0.2325252201858703 1104 70-71 0.18557131368053348 1104 72-73 0.21714476220541457 1104 74-75 0.3176796420744594 1104 76-77 0.373524307815881 1104 78-79 0.3660101028452942 1104 80-81 0.26294684900569365 1104 82-83 0.059304838671259574 1104 84-85 0.13891891122116462 1104 86-87 0.4133295123169063 1104 88-89 0.20590217565767688 1104 90-91 0.15382704253793023 1104 92-93 0.2756660692417796 1104 94-95 0.4450604755193801 1104 96-97 0.45295377089018274 1104 98-99 0.3592580618835406 1104 100-101 0.28930375043999135 1105 1 0.14695360235021582 1105 2 0.2410735844373484 1105 3 0.2511602470214207 1105 4 0.16760102404740707 1105 5 0.26081289734059254 1105 6 0.24991173882966677 1105 7 0.29603651810327136 1105 8 0.2701911043307561 1105 9 0.3094960809917495 1105 10-11 0.3184923083866451 1105 12-13 0.22663197547612413 1105 14-15 0.4566291496443142 1105 16-17 0.4559113021100174 1105 18-19 0.36671404939770014 1105 20-21 0.2822464523679429 1105 22-23 0.35427261864563064 1105 24-25 0.33565757797003926 1105 26-27 0.42456368414476486 1105 28-29 0.37550910083668754 1105 30-31 0.3327748331394247 1105 32-33 0.2635538116495866 1105 34-35 0.029387277728631034 1105 36-37 0.06983471171691491 1105 38-39 0.35682726622542305 1105 40-41 0.3984271648367539 1105 42-43 0.5095217264591199 1105 44-45 0.3548308425542004 1105 46-47 0.45358691542428176 1105 48-49 0.4758586528602393 1105 50-51 0.3833996921500642 1105 52-53 0.5141301165649068 1105 54-55 0.5394328189081676 1105 56-57 0.4305393666504429 1105 58-59 0.4082420200905261 1105 60-61 0.6170925866195702 1105 62-63 0.45454499518348257 1105 64-65 0.4579157642645342 1105 66-67 0.38426263422841345 1105 68-69 0.3894963974331347 1105 70-71 0.5501208191445812 1105 72-73 0.5492109384077324 1105 74-75 0.5450259577996874 1105 76-77 0.46949187307685847 1105 78-79 0.40826864426056986 1105 80-81 0.28699271079051414 1105 82-83 0.20605424436559971 1105 84-85 0.3830589539973843 1105 86-87 0.33607381149690596 1105 88-89 0.4297622680598252 1105 90-91 0.591018698400692 1105 92-93 0.551258201793253 1105 94-95 0.4787149303444984 1105 96-97 0.4045057749683991 1105 98-99 0.5490814506674688 1105 100-101 0.7250376151610745 1106 1 0.4420101136810288 1106 2 0.44854863356522756 1106 3 0.44934127864974727 1106 4 0.37806751369725333 1106 5 0.39598718871299354 1106 6 0.4005189708083918 1106 7 0.31951640834100203 1106 8 0.4114852892900771 1106 9 0.46652514160584246 1106 10-11 0.5269442062006604 1106 12-13 0.5834151746936271 1106 14-15 0.4790567074337915 1106 16-17 0.5421397654509192 1106 18-19 0.6779329662628015 1106 20-21 0.47237419119158375 1106 22-23 0.2785237118915447 1106 24-25 0.41720414510808723 1106 26-27 0.30284706921725046 1106 28-29 0.33625817885644693 1106 30-31 0.3928212144224972 1106 32-33 0.5029597607849539 1106 34-35 0.5429527881193792 1106 36-37 0.26757996734045975 1106 38-39 0.35730427285658095 1106 40-41 0.49628520208133153 1106 42-43 0.36550061130387945 1106 44-45 0.30505532745680597 1106 46-47 0.4664109088578243 1106 48-49 0.5463122785546588 1106 50-51 0.569155715944575 1106 52-53 0.44415730340561765 1106 54-55 0.574817576770215 1106 56-57 0.5672258705343722 1106 58-59 0.5825207061402367 1106 60-61 0.6249746137455148 1106 62-63 0.7122230892882584 1106 64-65 0.32731490083178727 1106 66-67 0.3358559759262789 1106 68-69 0.4004580351897289 1106 70-71 0.4755993164214516 1106 72-73 0.33686052629175833 1106 74-75 0.5536958070478377 1106 76-77 0.45496156602920834 1106 78-79 0.4266961969755094 1106 80-81 0.442304674395924 1106 82-83 0.5493582003490509 1106 84-85 0.6116364720151672 1106 86-87 0.7566274690340578 1106 88-89 0.7036522444505344 1106 90-91 0.5963000244791274 1106 92-93 0.7046367661809043 1106 94-95 0.9897812304603235 1106 96-97 0.9492200721003989 1106 98-99 0.84218933916463 1106 100-101 1.0954535624061705 1107 1 0.14869442510768494 1107 2 0.2957233418480314 1107 3 0.38021776365254567 1107 4 0.2725902246167067 1107 5 0.2780608840993537 1107 6 0.2437201864853975 1107 7 0.2938571702524726 1107 8 0.3730660719324632 1107 9 0.3912992903551924 1107 10-11 0.38923044379104255 1107 12-13 0.477034078068435 1107 14-15 0.3890696390972508 1107 16-17 0.436896755845261 1107 18-19 0.6471411157266687 1107 20-21 0.6736459221727316 1107 22-23 0.6060784198724463 1107 24-25 0.5001274054974445 1107 26-27 0.6614041572280343 1107 28-29 0.5908783049527457 1107 30-31 0.6695863864840703 1107 32-33 0.602213045276514 1107 34-35 0.6278572598584162 1107 36-37 0.5408988762502176 1107 38-39 0.654472490148784 1107 40-41 0.765424013676558 1107 42-43 0.8525216491579286 1107 44-45 0.7618186880791384 1107 46-47 0.6976515937831351 1107 48-49 0.6878999044119425 1107 50-51 0.6701226219560397 1107 52-53 0.688513412232858 1107 54-55 0.7961838044983125 1107 56-57 0.7847728798985045 1107 58-59 0.8312661335141058 1107 60-61 0.8260695281298567 1107 62-63 0.8819970116077087 1107 64-65 0.7456083237978177 1107 66-67 0.7658627688234318 1107 68-69 0.5944957835707569 1107 70-71 0.5942375166559373 1107 72-73 0.7014743626684847 1107 74-75 0.7927032389804864 1107 76-77 0.651707416072238 1107 78-79 0.6675087281096239 1107 80-81 0.8152575719439383 1107 82-83 0.810916943814032 1107 84-85 0.701972644947233 1107 86-87 0.8349628570091205 1107 88-89 0.8847026779380087 1107 90-91 0.8145313553334717 1107 92-93 0.8628371012971598 1107 94-95 1.0734442625077065 1107 96-97 1.008853557561082 1107 98-99 1.0533047328759189 1107 100-101 1.1503914954533627 1108 1 -0.003072701112955656 1108 2 0.052192254464507926 1108 3 0.01638599578160438 1108 4 0.05610921030878302 1108 5 -0.0066706910754206206 1108 6 0.022919054511795878 1108 7 0.05396217569823847 1108 8 0.0258897689061115 1108 9 -0.054325575828023887 1108 10-11 -0.17654196993786542 1108 12-13 0.03069288484165611 1108 14-15 0.08458912747496328 1108 16-17 0.1676571144276906 1108 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2316 50-51 -2.108771518029151 2316 52-53 -1.991271258043497 2316 54-55 -1.8265201275643221 2316 56-57 -2.0893824412703665 2316 58-59 -1.9397502144353638 2316 60-61 -2.2735595914595095 2316 62-63 -2.2265529555294705 2316 64-65 -2.565847447017916 2316 66-67 -2.3080075325755907 2316 68-69 -2.180032608863492 2316 70-71 -1.9629667440349117 2316 72-73 -2.301109698341609 2316 74-75 -2.830355298571803 2316 76-77 -2.8623549502595473 2316 78-79 -3.1879844750243436 2316 80-81 -2.9943076998785365 2316 82-83 -3.6231993592750733 2316 84-85 -3.4566469079097466 2316 86-87 -3.7751214387354075 2316 88-89 -3.7348551867088986 2316 90-91 -4.1385182101470015 2316 92-93 -4.1357669069237595 2316 94-95 -4.238435490864646 2316 96-97 -4.55480871696345 2316 98-99 -4.750904420723867 2316 100-101 -4.966772808219968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4671.0 3 1890.0 4 1769.0 5 1989.0 6 2224.0 7 2650.0 8 3102.0 9 3966.0 10 4895.0 11 6145.0 12 7821.0 13 9395.0 14 10528.0 15 12192.0 16 15184.0 17 19059.0 18 17414.0 19 11917.0 20 12029.0 21 12869.0 22 14012.0 23 15882.0 24 18123.0 25 20969.0 26 24217.0 27 28766.0 28 33950.0 29 41339.0 30 50151.0 31 62216.0 32 78789.0 33 101279.0 34 136586.0 35 192818.0 36 287805.0 37 425932.0 38 399746.0 39 72213.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45442112101745 19.17298978773429 14.558515862326265 22.814073228921995 2 15.02386260752255 20.524012426931296 40.77834991811873 23.673775047427423 3 19.086269715665978 25.00507838252593 29.93969651586025 25.96895538594784 4 12.511250127566864 17.07050141512636 38.264656566911306 32.153591890395475 5 13.650116492932115 37.50180756929169 34.27788935042543 14.570186587350756 6 30.91765206020027 36.814025165079414 18.019462966300235 14.24885980842009 7 27.781967648451122 32.884137118841714 21.35470090404221 17.97919432866495 8 25.627459123744657 35.015011713247866 20.170901160145114 19.186628002862367 9 25.94436599843601 17.262771649135253 20.94771708258448 35.84514526984426 10-11 24.325582705872627 26.969185793806115 27.9727457027737 20.732485797547557 12-13 25.55174951050288 24.39109052890631 28.220184325283824 21.83697563530698 14-15 22.47548698678206 25.672945979343925 26.799785970836332 25.051781063037687 16-17 21.773196933214003 29.046584593842965 27.142312207385817 22.037906265557215 18-19 21.61398292246756 27.74168384765452 29.432995071088648 21.211338158789275 20-21 22.4678931792403 26.80522354697239 29.45013374763078 21.27674952615653 22-23 22.007262955464185 26.81639682609308 29.343101923782832 21.833238294659903 24-25 22.0701109506498 26.912393278289233 29.403087765977702 21.614408005083266 26-27 21.9036547710457 27.018174038452887 29.407914581765404 21.67025660873601 28-29 21.582975572726628 27.278656757307562 29.481443493365283 21.65692417660053 30-31 21.823629034379902 26.92734665767484 29.438065999480244 21.810958308465015 32-33 21.69479774175911 27.078118692664916 29.384873774249304 21.84220979132667 34-35 22.18667128267267 27.055934452714787 29.119450695285902 21.63794356932664 36-37 22.14632538632223 26.843307602367254 29.178573950870362 21.831793060440152 38-39 21.829126889391713 27.18903102696685 29.241409170260123 21.74043291338132 40-41 22.06300067832255 27.049329213083524 29.167315792959986 21.720354315633937 42-43 21.920807594974686 27.107745106298026 28.88915301999947 22.082294278727815 44-45 22.072772759373542 27.193868366513268 28.703582325164902 22.02977654894829 46-47 22.167989394788417 27.378764282371144 28.43700063051528 22.01624569232516 48-49 22.231294639601426 27.40085704342175 28.251207698251296 22.11664061872553 50-51 21.95886260216209 27.56629847196236 28.36776297311089 22.107075952764667 52-53 22.34263260843224 27.745774936386034 27.901633479698347 22.00995897548338 54-55 22.086971695099592 27.51270849220872 28.058324270127244 22.341995542564447 56-57 22.175420099698524 27.811239372007325 27.916924975915496 22.096415552378655 58-59 22.468116769547564 27.50917296314982 27.957377628817298 22.06533263848532 60-61 22.320320779590045 27.491239212876973 28.354116739103947 21.83432326842904 62-63 22.073619198609997 27.595509165644007 28.31324592102703 22.017625714718967 64-65 21.983652234841554 27.65958134267509 28.12563827805879 22.23112814442457 66-67 22.055982561802026 27.817162073165917 27.817923828610564 22.30893153642149 68-69 21.948007899182677 27.61668507238724 28.143066361492664 22.292240666937424 70-71 22.090143463902642 27.576857806390354 28.056109956894094 22.276888772812914 72-73 22.1414293597508 27.42039898995844 28.086074632387476 22.35209701790329 74-75 21.989159974167112 27.932247299572392 27.77607743587826 22.302515290382235 76-77 22.076406967677244 27.708986689651578 27.81436239733082 22.40024394534036 78-79 22.14044781452881 27.723309384271182 27.78312048184984 22.35312231935017 80-81 22.08508320877115 27.88955783965752 27.703384334542307 22.32197461702902 82-83 22.1475653288216 27.804360156488606 27.813501816905006 22.234572697784785 84-85 22.09357800917938 27.76573068653802 27.680815042463593 22.459876261819005 86-87 22.163232599516995 27.69775561120285 27.853054507904268 22.285957281375886 88-89 22.137604453865972 27.681224037336595 27.607222293158195 22.573949215639235 90-91 22.070446836555664 27.659453622464635 27.85330375248691 22.41679578849279 92-93 22.23066220850686 27.585002334207758 27.89883899138213 22.28549646590325 94-95 22.050372996528548 27.598350136642292 27.774000110791047 22.57727675603811 96-97 21.975328703637448 27.66230210958465 27.88116857426572 22.481200612512183 98-99 21.92121433756102 27.697804521721142 27.6302289673107 22.750752173407136 100-101 21.825538789494775 27.640712311872594 27.813697718971465 22.720051179661166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 330.0 1 240.0 2 200.5 3 262.0 4 251.5 5 271.0 6 440.5 7 929.0 8 1673.0 9 2100.0 10 2010.5 11 1903.0 12 1990.5 13 2167.0 14 2383.0 15 2568.5 16 2684.0 17 2763.5 18 2969.0 19 3269.0 20 3756.0 21 4525.5 22 5457.0 23 6591.0 24 8054.5 25 10051.5 26 12849.0 27 16232.5 28 19335.0 29 22540.5 30 26765.0 31 31109.0 32 35700.5 33 40634.5 34 46276.0 35 50687.5 36 55369.5 37 60768.5 38 63709.0 39 65479.0 40 67437.0 41 71098.5 42 73956.5 43 76327.5 44 79915.5 45 82248.0 46 81798.5 47 83398.5 48 90284.0 49 90449.0 50 82721.0 51 76786.0 52 72669.5 53 69588.5 54 65925.0 55 60443.5 56 54664.5 57 48936.5 58 43553.5 59 38610.5 60 32675.0 61 25911.0 62 20091.5 63 15518.0 64 11805.5 65 9051.5 66 6726.0 67 5104.5 68 3652.5 69 2345.0 70 1701.0 71 1196.5 72 844.5 73 618.0 74 440.5 75 325.5 76 230.0 77 123.5 78 66.0 79 36.5 80 20.5 81 15.5 82 14.0 83 10.5 84 10.0 85 11.5 86 7.5 87 4.0 88 5.0 89 4.0 90 3.0 91 5.5 92 4.5 93 2.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03683314355186896 2 0.02704789739523209 3 0.02229374478139437 4 0.045972194207595844 5 0.0931444657740245 6 0.111561037549959 7 0.0577883405099498 8 0.08695945169388611 9 0.07182001424399512 10-11 0.07311240524581508 12-13 0.07154307331503369 14-15 0.06468878532323853 16-17 0.05229567875221495 18-19 0.061988611265864677 20-21 0.05792681097443051 22-23 0.03424836154822904 24-25 0.00939291317394152 26-27 0.002654017235880282 28-29 0.0072696993852372944 30-31 0.005354191293254135 32-33 0.006946601634782304 34-35 0.0070158368670226595 36-37 0.006277327723125537 38-39 0.004107957112927741 40-41 0.007085072099263014 42-43 0.004361819631142377 44-45 0.002630938825133497 46-47 0.0020770569672106556 48-49 4.154113934421311E-4 50-51 0.002561703592893142 52-53 0.0064388765983530325 54-55 0.011954616766834663 56-57 0.009046737012739744 58-59 0.004892623078318433 60-61 0.0025386251821463567 62-63 0.00939291317394152 64-65 0.0024693899499060017 66-67 0.02203988226317973 68-69 0.04553370440340693 70-71 0.059011496279529396 72-73 0.003761780951725965 74-75 0.013662419162096756 76-77 0.021601392458990818 78-79 0.024970840428021435 80-81 0.07435863942614147 82-83 0.02854799409377312 84-85 0.011977695177581446 86-87 0.03247132392072658 88-89 0.047887702299579005 90-91 0.04322586332872842 92-93 0.04200270755914881 94-95 0.013247007768654626 96-97 0.016893396666646663 98-99 0.03706392765933681 100-101 0.07405862008643327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2166527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.269084117088944 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02549832315405 47.627944058330435 2 12.494786971276202 15.06098733993905 3 3.8059491356717885 6.8814320580949495 4 1.6110808489481303 3.8839346881874377 5 0.8470260846221135 2.552474317172933 6 0.5052903357388764 1.8272031448919073 7 0.3233265666342483 1.3640617229238352 8 0.23383710846937394 1.1274518688030042 9 0.14900076583787603 0.8082125720814259 >10 0.8908712671562464 9.992711627473808 >50 0.07405543487851404 3.0505402125245373 >100 0.03713110430315722 3.9389686031459523 >500 0.0014562504598905075 0.5925188360641672 >1k 5.365133273280538E-4 0.6671668784029329 >5k 1.5328952209372966E-4 0.6243920719637501 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7931 0.3660697512655047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5586 0.2578320048630827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3798 0.17530360803257933 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3322 0.15333296100163996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3078410746785063E-5 0.0 12-13 0.0 0.0 0.0 4.6156821493570126E-5 0.0 14-15 0.0 0.0 0.0 4.6156821493570126E-5 0.0 16-17 0.0 0.0 0.0 4.6156821493570126E-5 4.6156821493570126E-5 18-19 0.0 0.0 0.0 6.923523224035519E-5 4.6156821493570126E-5 20-21 0.0 0.0 0.0 1.3847046448071037E-4 4.6156821493570126E-5 22-23 0.0 0.0 0.0 2.538625182146357E-4 6.923523224035519E-5 24-25 0.0 0.0 0.0 4.846466256824863E-4 9.231364298714025E-5 26-27 0.0 0.0 0.0 8.539011976310473E-4 9.231364298714025E-5 28-29 0.0 0.0 0.0 0.0015924103415281693 9.231364298714025E-5 30-31 0.0 0.0 0.0 0.0033002127367902636 1.1539205373392532E-4 32-33 0.0 0.0 0.0 0.005031093542799144 1.846272859742805E-4 34-35 0.0 0.0 0.0 0.0075697187249455 1.846272859742805E-4 36-37 0.0 0.0 0.0 0.010269892782319353 1.846272859742805E-4 38-39 0.0 0.0 0.0 0.015254829503624926 1.846272859742805E-4 40-41 0.0 0.0 0.0 0.023447665318733622 1.846272859742805E-4 42-43 0.0 0.0 0.0 0.030417345364262712 1.846272859742805E-4 44-45 0.0 0.0 0.0 0.03900251416206675 1.846272859742805E-4 46-47 0.0 0.0 0.0 0.056495949508129834 2.307841074678506E-4 48-49 0.0 0.0 0.0 0.06773513554181415 2.7694092896142074E-4 50-51 0.0 0.0 0.0 0.08137447629316413 2.7694092896142074E-4 52-53 0.0 0.0 0.0 0.09628312963558727 3.000193397082058E-4 54-55 0.0 0.0 0.0 0.11223031146161576 3.2309775045499085E-4 56-57 0.0 0.0 0.0 0.1619642866209376 3.461761612017759E-4 58-59 0.0 0.0 0.0 0.20629791366551167 3.69254571948561E-4 60-61 0.0 0.0 0.0 0.23671525902977436 3.69254571948561E-4 62-63 0.0 0.0 0.0 0.2804026905734385 3.69254571948561E-4 64-65 0.0 0.0 0.0 0.3265595120670086 3.69254571948561E-4 66-67 0.0 0.0 0.0 0.39671788073723524 3.69254571948561E-4 68-69 0.0 0.0 0.0 0.4810002367844943 3.69254571948561E-4 70-71 0.0 0.0 0.0 0.5217797885740635 3.69254571948561E-4 72-73 0.0 0.0 0.0 0.5687212760330242 4.154113934421311E-4 74-75 0.0 0.0 0.0 0.6629504271121477 4.154113934421311E-4 76-77 0.0 0.0 0.0 0.7735652498214884 4.154113934421311E-4 78-79 0.0 0.0 0.0 0.8788258812375751 4.154113934421311E-4 80-81 0.0 0.0 0.0 0.9533922263604377 4.3848980418891617E-4 82-83 0.0 0.0 0.0 1.064260911587993 4.615682149357012E-4 84-85 0.0 0.0 0.0 1.281174894197026 4.615682149357012E-4 86-87 0.0 0.0 0.0 1.5114743550391943 4.615682149357012E-4 88-89 0.0 0.0 0.0 1.780753251632682 5.077250364292714E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11550 0.0 28.149965 1 GGTATCA 8220 0.0 24.692202 1 GTACATG 8255 0.0 24.184437 1 ATCAACG 13410 0.0 23.96746 3 TCAACGC 13480 0.0 23.913542 4 TATCAAC 13685 0.0 23.624258 2 CAACGCA 13605 0.0 23.554043 5 TACATGG 8335 0.0 23.044636 2 AACGCAG 14015 0.0 22.827896 6 ACATGGG 8335 0.0 22.075445 3 GAGTACT 6620 0.0 21.424936 12-13 ACGCAGA 15480 0.0 20.386406 7 CGCAGAG 15590 0.0 20.181126 8 AGAGTAC 11150 0.0 20.1574 10-11 GTACTTT 7265 0.0 19.49099 14-15 AGTACTT 7050 0.0 19.410494 12-13 CATGGGG 4015 0.0 19.183752 4 GTGTAGC 1235 0.0 18.859617 1 GCAGAGT 16235 0.0 18.703888 9 TACGCTG 185 1.5111474E-4 17.98997 5 >>END_MODULE