FastQCFastQC Report
Thu 26 May 2016
SRR936868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166527
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87510.40391834489023215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71540.33020590096500063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46530.2147676904095818No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35980.1660722437338653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125200.027.0210761
GGTATCA91650.025.1441081
GTACATG79600.023.5185281
TACATGG81650.022.6889882
ACATGGG79450.022.5389733
ATCAACG149650.022.4085963
TCAACGC151400.022.1809524
CAACGCA151950.022.1006665
TATCAAC153250.022.0381852
AACGCAG154650.021.7782546
GAGTACT83100.021.00767712-13
AGAGTAC125350.020.38941210-11
AGTACTT87650.019.75455912-13
ACGCAGA172600.019.2656657
GTACTTT90800.019.14616814-15
CGCAGAG173950.019.0624088
GTACGCT1250.006033387518.9996973
GCAGAGT181750.017.9568069
TAGGACG1350.00940387517.5923124
CATGGGA46250.017.3564784
Produced by FastQC (version 0.11.5)