##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2166527 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11521388840296 31.0 31.0 34.0 31.0 34.0 2 32.24886788856082 33.0 31.0 34.0 30.0 34.0 3 32.37758910920566 34.0 31.0 34.0 31.0 34.0 4 36.045698484256135 37.0 35.0 37.0 35.0 37.0 5 35.881251422207065 37.0 35.0 37.0 35.0 37.0 6 35.96538838426662 37.0 35.0 37.0 35.0 37.0 7 35.92305934797951 37.0 35.0 37.0 35.0 37.0 8 35.87265286793102 37.0 35.0 37.0 35.0 37.0 9 37.56945055381262 39.0 37.0 39.0 35.0 39.0 10-11 37.47619115755308 39.0 37.0 39.0 35.0 39.0 12-13 37.37545389464336 39.0 37.0 39.0 34.0 39.0 14-15 38.57595612701803 40.0 38.0 41.0 34.0 41.0 16-17 38.575254774115436 40.0 38.0 41.0 34.0 41.0 18-19 38.697514039751184 40.0 38.0 41.0 34.0 41.0 20-21 38.69676306826548 40.0 38.0 41.0 34.0 41.0 22-23 38.61035888313416 40.0 38.0 41.0 34.0 41.0 24-25 38.52053793929178 40.0 38.0 41.0 34.0 41.0 26-27 38.331013414557034 40.0 38.0 41.0 34.0 41.0 28-29 38.18468198180775 40.0 38.0 41.0 34.0 41.0 30-31 38.175283760599335 40.0 38.0 41.0 34.0 41.0 32-33 38.057185532421244 40.0 37.0 41.0 33.5 41.0 34-35 37.93749812487913 40.0 37.0 41.0 33.0 41.0 36-37 37.794227350963084 40.0 37.0 41.0 33.0 41.0 38-39 37.64541960474068 40.0 37.0 41.0 33.0 41.0 40-41 37.31973984169133 40.0 36.5 41.0 32.0 41.0 42-43 37.13268470690649 40.0 36.0 41.0 31.0 41.0 44-45 36.93114810016215 39.0 36.0 41.0 31.0 41.0 46-47 36.58800628840536 39.0 36.0 41.0 30.5 41.0 48-49 36.24602578227735 39.0 35.5 40.0 30.0 41.0 50-51 35.97540649158769 39.0 35.0 40.0 29.0 41.0 52-53 35.72323515931258 39.0 35.0 40.0 28.5 41.0 54-55 35.337322821271094 38.0 35.0 40.0 27.5 41.0 56-57 35.15120374682614 38.0 34.0 40.0 27.0 41.0 58-59 34.83723258468507 38.0 34.0 40.0 26.5 41.0 60-61 34.46887276272117 37.5 34.0 40.0 26.0 41.0 62-63 34.09990413228176 37.0 33.0 39.5 26.0 41.0 64-65 33.78095818792011 36.5 33.0 39.0 25.0 40.0 66-67 33.44806872935348 36.0 32.5 39.0 24.5 40.0 68-69 32.999273030061474 35.5 32.0 39.0 23.0 40.0 70-71 32.55188580617735 35.0 31.5 38.0 22.0 40.0 72-73 32.134824767935044 35.0 31.0 37.5 22.0 39.5 74-75 31.64260842352761 35.0 31.0 37.0 20.5 39.0 76-77 30.202349197586734 33.5 29.5 35.5 19.5 37.5 78-79 30.88842811559699 34.0 30.5 36.0 20.0 38.0 80-81 30.746324186128305 34.0 30.5 35.5 18.5 37.0 82-83 30.38409722103625 34.0 30.0 35.0 18.0 37.0 84-85 29.92541496136443 34.0 30.0 35.0 13.5 36.5 86-87 29.540434298764797 34.0 29.0 35.0 8.5 36.0 88-89 29.146041337126192 34.0 29.0 35.0 6.0 35.5 90-91 28.683141036322187 33.5 29.0 35.0 2.0 35.0 92-93 28.184428119289535 33.0 28.5 35.0 2.0 35.0 94-95 27.601449462665364 33.0 27.0 34.5 2.0 35.0 96-97 26.79415950043549 32.0 25.5 34.0 2.0 35.0 98-99 25.896149921048757 32.0 24.5 34.0 2.0 35.0 100-101 22.62778885285067 28.0 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25569768778036917 1101 2 0.3489693846174333 1101 3 0.2702590281648938 1101 4 0.18752436970582664 1101 5 0.10023890714158767 1101 6 0.19006915345570974 1101 7 0.21633165676588817 1101 8 0.22137332965152723 1101 9 0.25697274825567007 1101 10-11 0.32377651495407633 1101 12-13 0.275212675039576 1101 14-15 0.4305466672977474 1101 16-17 0.464811974546933 1101 18-19 0.2721632779987644 1101 20-21 0.3190895958248561 1101 22-23 0.2804961635100156 1101 24-25 0.23470846446306837 1101 26-27 0.2548566015037963 1101 28-29 0.35788038741448247 1101 30-31 0.31602188377906515 1101 32-33 0.29495476766094697 1101 34-35 0.2185811657778416 1101 36-37 0.24458911025455876 1101 38-39 0.2768407596019742 1101 40-41 0.3656101133816847 1101 42-43 0.3416651568164113 1101 44-45 0.2083555923204159 1101 46-47 0.1574475545953007 1101 48-49 0.2113165141535518 1101 50-51 0.12758545024892953 1101 52-53 0.29104022906960125 1101 54-55 0.12557683397449892 1101 56-57 0.1132428259892535 1101 58-59 0.1369533621804493 1101 60-61 0.22754584611573847 1101 62-63 0.2973758107246951 1101 64-65 0.3242149938091785 1101 66-67 0.22623788427819136 1101 68-69 -0.04139566500826675 1101 70-71 -0.014534079303409442 1101 72-73 0.09728336827066641 1101 74-75 -0.027766480471012045 1101 76-77 0.07543055311590763 1101 78-79 0.0292267077587276 1101 80-81 0.06957013904279918 1101 82-83 0.3628849703113488 1101 84-85 0.015492078510270346 1101 86-87 -0.13047597828106916 1101 88-89 0.003817156161556312 1101 90-91 0.21843219487069732 1101 92-93 0.20869291445417204 1101 94-95 0.1664680067535258 1101 96-97 0.0515758575250409 1101 98-99 0.21009478717529717 1101 100-101 0.3805856815914339 1102 1 -0.026488153838236883 1102 2 0.12581204694587456 1102 3 0.13573705851617746 1102 4 0.11422870189417011 1102 5 0.030205929392991493 1102 6 0.10956483400765649 1102 7 0.1521537239268156 1102 8 0.2404769033026497 1102 9 0.2317001305672477 1102 10-11 0.2803794638410153 1102 12-13 0.33109266151526384 1102 14-15 0.3025036247206856 1102 16-17 0.204430647890824 1102 18-19 0.30434181845162556 1102 20-21 0.2978801503704389 1102 22-23 0.24994681203973812 1102 24-25 0.19991816472326462 1102 26-27 0.3302820536615698 1102 28-29 0.1180743338170629 1102 30-31 0.253360927375347 1102 32-33 0.2154875077541476 1102 34-35 0.40509605966593654 1102 36-37 0.2583961536744468 1102 38-39 0.378571870898206 1102 40-41 0.4104962102960883 1102 42-43 0.34497674998957706 1102 44-45 0.4201067450715854 1102 46-47 0.2966051274722119 1102 48-49 0.43001867069251176 1102 50-51 0.3925550450587423 1102 52-53 0.45398058981579226 1102 54-55 0.5874235878958061 1102 56-57 0.26591127956314864 1102 58-59 0.20785238558828212 1102 60-61 0.27519707579865127 1102 62-63 0.3933916282837515 1102 64-65 0.5115255677592074 1102 66-67 0.37312675465555856 1102 68-69 0.2879211124825858 1102 70-71 0.12058668393790839 1102 72-73 0.0050406363606469995 1102 74-75 0.14850806296112395 1102 76-77 0.21173528123323848 1102 78-79 -0.1855124348198096 1102 80-81 0.12489014080683702 1102 82-83 0.2325007525896048 1102 84-85 0.2648797116095416 1102 86-87 0.1683775916949699 1102 88-89 0.11420980207461895 1102 90-91 0.21146129663130608 1102 92-93 0.31725781887750415 1102 94-95 0.22155446921622612 1102 96-97 0.5418317903347152 1102 98-99 0.761520866547297 1102 100-101 0.6445462454962332 1103 1 0.20619427438429483 1103 2 0.26020274604837823 1103 3 0.23255938849354152 1103 4 0.14525883805897166 1103 5 0.13244691588580793 1103 6 0.14746163379510335 1103 7 0.17629868067356114 1103 8 0.15815927220272386 1103 9 0.25061410546270935 1103 10-11 0.21487723097990852 1103 12-13 0.2609141117226983 1103 14-15 0.279134623613686 1103 16-17 0.195596546181946 1103 18-19 0.305844743767544 1103 20-21 0.2420426234191737 1103 22-23 0.30542534827802825 1103 24-25 0.254244946580954 1103 26-27 0.30041733401767345 1103 28-29 0.13753200796710985 1103 30-31 0.20240896382019002 1103 32-33 0.24995268349780986 1103 34-35 0.32387679274043535 1103 36-37 0.31693689687330817 1103 38-39 0.2521126734195178 1103 40-41 0.30041072844138483 1103 42-43 0.19479297622599034 1103 44-45 0.10243277119719352 1103 46-47 0.16774804460521153 1103 48-49 0.1564106720127043 1103 50-51 0.047397968195895146 1103 52-53 -0.09339449957235502 1103 54-55 0.14717154297900237 1103 56-57 0.03840904957827718 1103 58-59 -0.005017422483817313 1103 60-61 0.10442571495240571 1103 62-63 0.2712466111359717 1103 64-65 0.0975991189701233 1103 66-67 0.22081896748724716 1103 68-69 0.14661835909392806 1103 70-71 0.06790744834185602 1103 72-73 0.28321925014956406 1103 74-75 0.15579762375567086 1103 76-77 0.2035608272065197 1103 78-79 0.2002612863409432 1103 80-81 0.010177417866774618 1103 82-83 0.25168356604854125 1103 84-85 0.18830143779220876 1103 86-87 -0.023333875406773785 1103 88-89 0.19043636566163613 1103 90-91 0.22385240847426147 1103 92-93 0.28162102472029815 1103 94-95 0.1413962652268097 1103 96-97 0.40251984448281064 1103 98-99 0.2846784640095912 1103 100-101 0.7644211901735574 1104 1 0.12425820649217911 1104 2 0.1984686447342341 1104 3 0.08400377079289711 1104 4 0.005004098773206067 1104 5 -0.08652385876123247 1104 6 0.028172811793048425 1104 7 0.1345535096948467 1104 8 0.1790361389670494 1104 9 0.12773620502735383 1104 10-11 0.2378279473399587 1104 12-13 0.17360444436406652 1104 14-15 0.2889028662119699 1104 16-17 0.1622270046790888 1104 18-19 0.21349824203156942 1104 20-21 0.24907778570175765 1104 22-23 0.1930915430911071 1104 24-25 0.16530368533103967 1104 26-27 0.13028734423450317 1104 28-29 0.19723190429175474 1104 30-31 0.2079483342535653 1104 32-33 0.19858294950883248 1104 34-35 0.21029354026610747 1104 36-37 0.1520467595265771 1104 38-39 0.08557287520114443 1104 40-41 0.3877705723426743 1104 42-43 0.07249321916265927 1104 44-45 0.11780023645293625 1104 46-47 0.1256962487131119 1104 48-49 0.2180987924336648 1104 50-51 0.25034444438121284 1104 52-53 0.19312880032478574 1104 54-55 0.31394689694625555 1104 56-57 0.22029893107600884 1104 58-59 -0.028306017064544164 1104 60-61 0.09089842730450215 1104 62-63 0.07726349785446018 1104 64-65 0.023893459706862075 1104 66-67 -0.11114484306666839 1104 68-69 -0.017693634880778575 1104 70-71 0.11490094868144496 1104 72-73 0.17497166504552553 1104 74-75 -0.0386480384736565 1104 76-77 0.2697810196857944 1104 78-79 0.39151423057374046 1104 80-81 0.3383296093144317 1104 82-83 0.32955250834414684 1104 84-85 0.28027093045427165 1104 86-87 0.33129217919968923 1104 88-89 0.4520668772465939 1104 90-91 0.3333596303001336 1104 92-93 0.2433525915196597 1104 94-95 0.4289683918662348 1104 96-97 0.8161017954495371 1104 98-99 0.6820848162571416 1104 100-101 1.2850530823840671 1105 1 -8.835080235414239E-4 1105 2 0.10630658604159748 1105 3 0.07065608344433372 1105 4 0.06752964437514208 1105 5 0.005432910388236678 1105 6 0.061086391620669644 1105 7 0.0623373224882684 1105 8 0.1664176414208569 1105 9 0.16033571371113453 1105 10-11 0.06951935663670383 1105 12-13 0.11682044881082732 1105 14-15 0.11829952175779823 1105 16-17 0.10167381913517204 1105 18-19 0.14625692156753445 1105 20-21 0.19235011447035077 1105 22-23 0.15425995836175588 1105 24-25 0.06782292933422696 1105 26-27 0.02958366010265223 1105 28-29 0.06408221626965371 1105 30-31 0.14931950699487828 1105 32-33 0.31200113473245494 1105 34-35 0.24418968527820084 1105 36-37 0.28463749170409613 1105 38-39 0.23555765082101487 1105 40-41 0.24420332269966138 1105 42-43 0.36800003465780406 1105 44-45 0.42086283362289834 1105 46-47 0.19554076163971246 1105 48-49 0.3070688593351889 1105 50-51 0.2533949528385264 1105 52-53 0.2740196896891973 1105 54-55 0.4589295683903529 1105 56-57 0.18962840431581185 1105 58-59 0.17584913744315855 1105 60-61 0.2583205404350153 1105 62-63 0.21495746578860775 1105 64-65 0.33490829452443904 1105 66-67 0.29251719620667416 1105 68-69 0.21875948302351844 1105 70-71 0.1653088628707522 1105 72-73 -0.04990100415736265 1105 74-75 0.012022078043710138 1105 76-77 -0.027650490359185653 1105 78-79 0.2225181468794979 1105 80-81 0.11384725925524108 1105 82-83 0.17273887106571806 1105 84-85 -0.09933356183913489 1105 86-87 0.04829020381324867 1105 88-89 0.27335594089593584 1105 90-91 0.3087258939144384 1105 92-93 0.26912971163858757 1105 94-95 0.454057346464964 1105 96-97 0.5609230262553453 1105 98-99 0.567537118215359 1105 100-101 0.9110112473679237 1106 1 -0.018744615363985417 1106 2 0.06922439500805666 1106 3 0.07316417416186027 1106 4 0.07029526792810259 1106 5 -0.06622591823063573 1106 6 0.13268940031462506 1106 7 0.16126672170095446 1106 8 0.15302973988212898 1106 9 0.17839472805812306 1106 10-11 0.19156137635381754 1106 12-13 0.2953192480481235 1106 14-15 0.38466522599593134 1106 16-17 0.12552668796215016 1106 18-19 0.16575329898126512 1106 20-21 0.0513077534905122 1106 22-23 0.19339099436687945 1106 24-25 0.22108795092004385 1106 26-27 0.2602411046411106 1106 28-29 0.3291469356666994 1106 30-31 0.23326950249123257 1106 32-33 0.16100316327927544 1106 34-35 0.24966194087256355 1106 36-37 0.2736536528409985 1106 38-39 0.3864674521655118 1106 40-41 0.44264569306739787 1106 42-43 0.33864065710071145 1106 44-45 0.14809877049056297 1106 46-47 0.15523941016321174 1106 48-49 0.07244946876190994 1106 50-51 0.24642190244455264 1106 52-53 0.33784469929910443 1106 54-55 0.5765009195750608 1106 56-57 0.5156199374481147 1106 58-59 0.47164272550987363 1106 60-61 0.35008025088183814 1106 62-63 0.2720821814558647 1106 64-65 0.33201263591909935 1106 66-67 0.2241866306112854 1106 68-69 0.20162340038972104 1106 70-71 0.37842616463706946 1106 72-73 0.40106816928909694 1106 74-75 0.1990029685998671 1106 76-77 0.37776962435075845 1106 78-79 0.3111398895014368 1106 80-81 0.3390229334394128 1106 82-83 0.4734078221205209 1106 84-85 0.5248093168705132 1106 86-87 0.45843217671774994 1106 88-89 0.6670836842866628 1106 90-91 0.5814239880445875 1106 92-93 0.7320031183920399 1106 94-95 0.7999372651201142 1106 96-97 0.6886326713040525 1106 98-99 0.6753970710982671 1106 100-101 1.1303689630136162 1107 1 0.20311341811744654 1107 2 0.2766098340556411 1107 3 0.23565228659185777 1107 4 0.13628841464964836 1107 5 0.048104075680910796 1107 6 0.08759724334335317 1107 7 0.14621929721533178 1107 8 0.2101373745953481 1107 9 0.25966937746915164 1107 10-11 0.18242820034734564 1107 12-13 0.18150480987103634 1107 14-15 0.3507713863988755 1107 16-17 0.2524524239851331 1107 18-19 0.3631745139538154 1107 20-21 0.2806626295327206 1107 22-23 0.10409166912799606 1107 24-25 0.23560734086755986 1107 26-27 0.3496880621779539 1107 28-29 0.31608263460549324 1107 30-31 0.19040390625097103 1107 32-33 0.2637188126047647 1107 34-35 0.33273846914863014 1107 36-37 0.32548798665905565 1107 38-39 0.3963913213997259 1107 40-41 0.3853853942805614 1107 42-43 0.2944741455804589 1107 44-45 0.2744230080507606 1107 46-47 0.3129531725728256 1107 48-49 0.33064235685018417 1107 50-51 0.29657421429387654 1107 52-53 0.4033997796313997 1107 54-55 0.5725431261093306 1107 56-57 0.39616417430386264 1107 58-59 0.23560504757371348 1107 60-61 0.23990539667753552 1107 62-63 0.2996230017359309 1107 64-65 0.4123048814496286 1107 66-67 0.34219294045797 1107 68-69 0.2947841102726372 1107 70-71 0.0045670442920950904 1107 72-73 0.15391258175380784 1107 74-75 0.09066048582112174 1107 76-77 -0.04277169407509973 1107 78-79 0.39057502236547137 1107 80-81 0.3918173300540353 1107 82-83 0.5777164309855038 1107 84-85 0.2885257863754376 1107 86-87 0.4884004262133139 1107 88-89 0.48921041458852343 1107 90-91 0.3871962398145179 1107 92-93 0.576333363892374 1107 94-95 0.8873668831580233 1107 96-97 0.8034859698845906 1107 98-99 0.8781846748157456 1107 100-101 1.3426081535015442 1108 1 0.01052337687860927 1108 2 0.06920881192816353 1108 3 -0.0171436695814009 1108 4 -0.00132289763915594 1108 5 -0.04279731415180521 1108 6 0.05805906250684245 1108 7 -0.0076451452225114735 1108 8 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-0.796902677863006 2316 80-81 -0.8877101756056724 2316 82-83 -0.8341394316374675 2316 84-85 -0.6605282860062296 2316 86-87 -0.701812191023496 2316 88-89 -1.2351722338418867 2316 90-91 -1.0867736933122494 2316 92-93 -1.0967080641276148 2316 94-95 -1.4500951681079783 2316 96-97 -1.369611377094639 2316 98-99 -1.6987910066965703 2316 100-101 -2.48982348661405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 14.0 5 48.0 6 95.0 7 224.0 8 519.0 9 1154.0 10 1858.0 11 3190.0 12 4666.0 13 6484.0 14 8352.0 15 11323.0 16 14939.0 17 19976.0 18 17982.0 19 11903.0 20 10642.0 21 10873.0 22 11968.0 23 13246.0 24 15335.0 25 18529.0 26 22526.0 27 28451.0 28 35651.0 29 44554.0 30 55623.0 31 70185.0 32 88712.0 33 115951.0 34 156747.0 35 221791.0 36 337147.0 37 484900.0 38 305499.0 39 15463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38881489500692 19.76653840205531 15.275461140901022 22.569185562036754 2 15.370380798782893 21.04709741207489 39.943298840589605 23.639222948552607 3 19.363514719206766 24.899112541154654 30.287331359971713 25.450041379666864 4 12.924140802307102 17.65678433732882 37.733063100529094 31.68601175983498 5 13.595893398594852 37.5342205244216 34.42163563374957 14.44825044323397 6 31.122529283041477 37.06443538437324 17.608688929332523 14.204346403252762 7 27.71855325591271 33.11250300020309 21.23299115632443 17.935952587559772 8 25.924486516900092 35.120817788100496 19.91449910386531 19.040196591134105 9 25.864079175601862 17.12409636441012 20.77925674559179 36.232567714396225 10-11 24.379207916450596 27.065097057432713 27.820961340652882 20.734733685463805 12-13 25.645156339326164 24.3604174403219 28.30261446039235 21.691811759959585 14-15 22.412752952623702 25.68294496605758 26.881926901128704 25.022375180190014 16-17 21.663819560060873 29.063681181910034 27.351840064767252 21.920659193261844 18-19 21.504572317951958 27.514128674794 29.895717363730522 21.08558164352352 20-21 22.27014372450388 26.521620526322103 30.056044155889715 21.152191593284304 22-23 21.899857326762334 26.591688210740973 29.903382846624194 21.6050716158725 24-25 21.950877837561393 26.584628692043495 30.052060519257406 21.412432951137703 26-27 21.836352957331393 26.816268928663327 29.90492828945926 21.442449824546017 28-29 21.506306181558024 26.970009577549302 30.051211040721892 21.47247320017078 30-31 21.74207845090322 26.67949672448116 29.99547201581148 21.582952808804137 32-33 21.659936220386307 26.843149138042843 29.844294290898365 21.652620350672482 34-35 22.10500953830716 26.708829384540326 29.70839966453222 21.477761412620293 36-37 22.03045291651204 26.580181233071716 29.77520803215521 21.61415781826104 38-39 21.763357025558292 26.915983192714844 29.79948738011076 21.521172401616102 40-41 22.01591818017541 26.722344371713763 29.678472331043864 21.58326511706696 42-43 21.871294472674467 26.876055548811532 29.394325572679225 21.858324405834775 44-45 22.019884094455332 26.875814531742474 29.22235830926808 21.881943064534106 46-47 22.153632979701783 27.157208990175725 28.844892074642264 21.84426595548023 48-49 22.256165459395703 27.26065305947241 28.60527143576201 21.877910045369877 50-51 22.02778675282714 27.352388645280406 28.584652665589662 22.035171936302792 52-53 22.357000545344484 27.562167078502142 28.229481327317234 21.85135104883614 54-55 22.14232732848471 27.317799408915743 28.36032045758027 22.179552805019277 56-57 22.21598077840446 27.64911402910149 28.213336447388127 21.921568745105922 58-59 22.476839046518535 27.31385096907557 28.215941345662763 21.99336863874313 60-61 22.292540727715757 27.29839927828011 28.687696212978448 21.721363781025683 62-63 22.109607244591746 27.405411380843947 28.5890571843917 21.89592419017261 64-65 22.03404068727571 27.43240904212968 28.446290719008548 22.087259551586065 66-67 22.06755327766513 27.547660379953726 28.171608292903805 22.213178049477346 68-69 21.99745121914069 27.44799739306152 28.370764994281167 22.183786393516623 70-71 22.15281237948701 27.34909609629639 28.33496305941396 22.163128464802647 72-73 22.170821330603303 27.297589084066114 28.3460211094192 22.18556847591138 74-75 22.083709521673065 27.690877501998646 27.973203389110758 22.252209587217536 76-77 22.135083171226544 27.48263405283439 28.0538484563641 22.32843431957497 78-79 22.174654965583102 27.498669527164033 28.007618659021855 22.319056848231014 80-81 22.132830400481517 27.682816276265964 27.920573904727085 22.26377941852543 82-83 22.160043812085295 27.52280090864382 28.09103793147138 22.226117347799505 84-85 22.104584153594416 27.555833527580386 27.86714424517133 22.47243807365387 86-87 22.150281488155436 27.441367981201196 27.977457869090582 22.430892661552786 88-89 22.127965492451473 27.476866302866515 27.821180184931105 22.57398801975091 90-91 21.9989822305767 27.438786708069802 28.05270245476292 22.509528606590575 92-93 22.130511182182357 27.258072481903064 28.081210158008645 22.530206177905928 94-95 21.928598166558736 27.295390272080617 28.057254767653482 22.718756793707165 96-97 21.94842195269147 27.343311689585498 28.06663674159858 22.641629616124455 98-99 21.847783190977214 27.34181392697489 27.783329448446008 23.027073433601892 100-101 21.498188659580393 27.428658204897832 27.98801932804198 23.085133807479796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 404.0 1 314.5 2 258.0 3 302.5 4 274.5 5 283.0 6 492.0 7 1042.5 8 1771.5 9 2216.0 10 2164.0 11 1984.5 12 2065.0 13 2259.5 14 2493.5 15 2771.5 16 2960.5 17 3058.5 18 3373.5 19 3736.5 20 4141.0 21 4779.0 22 5699.5 23 6925.5 24 8281.0 25 10288.5 26 13041.0 27 16407.5 28 19626.0 29 22881.0 30 27087.0 31 31332.5 32 35676.5 33 40702.5 34 46213.5 35 50422.0 36 55239.5 37 60720.0 38 63816.0 39 65056.5 40 67114.0 41 70845.0 42 74026.5 43 77124.5 44 80345.0 45 82238.0 46 81587.5 47 83315.5 48 90439.5 49 90163.0 50 82235.5 51 76433.5 52 71819.0 53 68676.5 54 65402.5 55 60077.0 56 54432.5 57 48575.0 58 43016.5 59 38266.5 60 32780.5 61 26204.5 62 20101.5 63 15416.5 64 11631.0 65 8816.5 66 6614.5 67 5057.0 68 3629.0 69 2288.5 70 1565.0 71 1093.0 72 800.0 73 615.5 74 499.0 75 385.0 76 235.0 77 122.5 78 69.5 79 38.0 80 25.0 81 15.5 82 13.0 83 8.0 84 5.0 85 4.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019939746885222296 2 0.0037387025409791803 3 1.846272859742805E-4 4 0.0 5 0.002954036575588488 6 0.0 7 3.230977504549909E-4 8 0.0 9 4.6156821493570126E-5 10-11 2.3078410746785063E-5 12-13 0.006161935669391611 14-15 0.002954036575588488 16-17 0.0 18-19 5.077250364292714E-4 20-21 9.692932513649727E-4 22-23 0.0017539592167556649 24-25 4.6156821493570127E-4 26-27 2.0770569672106556E-4 28-29 9.231364298714025E-5 30-31 0.0 32-33 1.846272859742805E-4 34-35 0.0 36-37 0.0016154887522749541 38-39 0.00943906999543509 40-41 4.846466256824863E-4 42-43 0.0 44-45 9.923716621117577E-4 46-47 3.000193397082058E-4 48-49 6.923523224035519E-5 50-51 0.0012462341803263935 52-53 3.000193397082058E-4 54-55 0.0 56-57 2.538625182146357E-4 58-59 1.6154887522749545E-4 60-61 0.0016385671630217394 62-63 2.3078410746785063E-5 64-65 9.231364298714025E-5 66-67 0.0 68-69 4.6156821493570126E-5 70-71 3.4617616120177595E-4 72-73 2.7694092896142074E-4 74-75 0.002723252468120637 76-77 1.1539205373392532E-4 78-79 1.846272859742805E-4 80-81 0.0016154887522749541 82-83 6.923523224035519E-5 84-85 0.0019385865027299453 86-87 2.7694092896142074E-4 88-89 0.0020539785564638703 90-91 0.001130842126592468 92-93 0.0 94-95 0.0 96-97 2.0770569672106556E-4 98-99 5.538818579228415E-4 100-101 0.0017308808060088796 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2166527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.36351246419984 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85534465437713 45.439113781403144 2 12.985605058951544 15.1577104542659 3 4.043248625858906 7.079345747135256 4 1.7510406070805464 4.0878752118666215 5 0.944086164398 2.7550092311560648 6 0.558266304941963 1.9549429468093835 7 0.3452296619366065 1.410417097421404 8 0.245018301202047 1.1440102940930195 9 0.1758366624514556 0.9236200716583944 >10 0.9729221913347743 10.437967166476913 >50 0.0815646617323221 3.27694355253493 >100 0.03906076294234413 4.040080348249819 >500 0.0019037779148122248 0.7301855462923527 >1k 7.139167180545593E-4 0.826435593891987 >5k 1.5864815956767985E-4 0.7363429567448353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8751 0.40391834489023215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7154 0.33020590096500063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4653 0.2147676904095818 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3598 0.1660722437338653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.6156821493570126E-5 7 0.0 0.0 0.0 0.0 4.6156821493570126E-5 8 0.0 0.0 0.0 4.6156821493570126E-5 4.6156821493570126E-5 9 0.0 0.0 0.0 4.6156821493570126E-5 1.3847046448071037E-4 10-11 0.0 0.0 0.0 6.923523224035519E-5 1.3847046448071037E-4 12-13 0.0 0.0 0.0 9.231364298714025E-5 1.3847046448071037E-4 14-15 0.0 0.0 0.0 1.3847046448071037E-4 1.3847046448071037E-4 16-17 0.0 0.0 0.0 1.846272859742805E-4 1.3847046448071037E-4 18-19 0.0 0.0 0.0 2.0770569672106556E-4 1.3847046448071037E-4 20-21 0.0 0.0 0.0 2.7694092896142074E-4 1.3847046448071037E-4 22-23 0.0 0.0 0.0 3.9233298269534606E-4 1.3847046448071037E-4 24-25 0.0 0.0 0.0 6.692739116567667E-4 1.6154887522749542E-4 26-27 0.0 0.0 0.0 0.0010616068943521129 1.846272859742805E-4 28-29 0.0 0.0 0.0 0.001800116038249235 1.846272859742805E-4 30-31 0.0 0.0 0.0 0.0034386832012709744 1.846272859742805E-4 32-33 0.0 0.0 0.0 0.005354191293254134 1.846272859742805E-4 34-35 0.0 0.0 0.0 0.007777424421666566 1.846272859742805E-4 36-37 0.0 0.0 0.0 0.010408363246800063 1.846272859742805E-4 38-39 0.0 0.0 0.0 0.015577927254079917 1.846272859742805E-4 40-41 0.0 0.0 0.0 0.024024625587403252 1.846272859742805E-4 42-43 0.0 0.0 0.0 0.03129432497264054 1.846272859742805E-4 44-45 0.0 0.0 0.0 0.0400179642349253 1.846272859742805E-4 46-47 0.0 0.0 0.0 0.057488321170241594 1.846272859742805E-4 48-49 0.0 0.0 0.0 0.06888905607915341 2.307841074678506E-4 50-51 0.0 0.0 0.0 0.08271302411647766 2.7694092896142074E-4 52-53 0.0 0.0 0.0 0.09769091269114116 2.7694092896142074E-4 54-55 0.0 0.0 0.0 0.11352270246343571 2.7694092896142074E-4 56-57 0.0 0.0 0.0 0.16334899126574465 2.7694092896142074E-4 58-59 0.0 0.0 0.0 0.2079364808285334 2.7694092896142074E-4 60-61 0.0 0.0 0.0 0.23863076712175754 2.7694092896142074E-4 62-63 0.0 0.0 0.0 0.2830797862200656 2.7694092896142074E-4 64-65 0.0 4.6156821493570126E-5 0.0 0.3291904508921421 2.7694092896142074E-4 66-67 0.0 4.6156821493570126E-5 0.0 0.3994642116161026 2.7694092896142074E-4 68-69 0.0 4.6156821493570126E-5 0.0 0.48420813587829736 2.7694092896142074E-4 70-71 0.0 4.6156821493570126E-5 0.0 0.5251723149538409 2.7694092896142074E-4 72-73 0.0 4.6156821493570126E-5 0.0 0.5721368808235485 4.615682149357012E-4 74-75 0.0 4.6156821493570126E-5 0.0 0.6670814626358221 4.615682149357012E-4 76-77 0.0 4.6156821493570126E-5 0.0 0.7781578535600987 4.846466256824863E-4 78-79 0.0 4.6156821493570126E-5 0.0 0.8845724055135247 5.077250364292714E-4 80-81 0.0 4.6156821493570126E-5 0.0 0.9596003188513229 5.077250364292714E-4 82-83 0.0 4.6156821493570126E-5 0.0 1.0699843574531958 5.538818579228415E-4 84-85 0.0 4.6156821493570126E-5 0.0 1.2871291241696965 5.538818579228415E-4 86-87 0.0 4.6156821493570126E-5 0.0 1.5166669974572207 5.538818579228415E-4 88-89 0.0 4.6156821493570126E-5 0.0 1.7857843451754811 5.538818579228415E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12520 0.0 27.021076 1 GGTATCA 9165 0.0 25.144108 1 GTACATG 7960 0.0 23.518528 1 TACATGG 8165 0.0 22.688988 2 ACATGGG 7945 0.0 22.538973 3 ATCAACG 14965 0.0 22.408596 3 TCAACGC 15140 0.0 22.180952 4 CAACGCA 15195 0.0 22.100666 5 TATCAAC 15325 0.0 22.038185 2 AACGCAG 15465 0.0 21.778254 6 GAGTACT 8310 0.0 21.007677 12-13 AGAGTAC 12535 0.0 20.389412 10-11 AGTACTT 8765 0.0 19.754559 12-13 ACGCAGA 17260 0.0 19.265665 7 GTACTTT 9080 0.0 19.146168 14-15 CGCAGAG 17395 0.0 19.062408 8 GTACGCT 125 0.0060333875 18.999697 3 GCAGAGT 18175 0.0 17.956806 9 TAGGACG 135 0.009403875 17.592312 4 CATGGGA 4625 0.0 17.356478 4 >>END_MODULE