FastQCFastQC Report
Thu 26 May 2016
SRR936867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855174
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66360.3577022963883711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50390.27161872686874655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.1743771743243491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.047.7850841
GTATCAA65150.040.5564461
ATCAACG80450.032.3117753
TCAACGC80750.032.251454
TATCAAC82200.031.7973122
CAACGCA81900.031.7388525
AACGCAG84350.030.8733166
ACGCAGA95350.027.01267
CGCAGAG96050.026.7182278
GCAGAGT105400.023.4920049
GAGTACT56400.022.70311412-13
GTACATG67350.021.944331
GTACTTT60250.021.2126614-15
TACATGG68350.020.9279882
AGAGTAC93550.020.21611810-11
CAGAGTA100900.019.99148410-11
AGTACTT60600.019.79677812-13
ACATGGG69300.019.749623
GCACCGT5550.018.837256
GTATAGG5850.018.684071
Produced by FastQC (version 0.11.5)