FastQCFastQC Report
Thu 26 May 2016
SRR936867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855174
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78910.425350937432284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64230.34622089356577873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.2300592828489403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.050.0187151
GTATCAA71000.038.406551
ATCAACG90900.029.7862553
TCAACGC92500.029.3223884
TATCAAC92450.029.184892
CAACGCA93950.028.9203935
AACGCAG97000.028.0102846
ACGCAGA111100.024.2837547
CGCAGAG112750.023.8019988
GTACATG71750.023.7697331
ACATGGG69250.023.4590973
TACATGG72250.022.9458542
GAGTACT73050.021.8796612-13
GCAGAGT123950.021.3830289
GTACTTT77150.020.74852414-15
AGTACTT76300.020.1384212-13
AGAGTAC110900.019.68047310-11
CAGAGTA118650.019.21564710-11
TACGGGT1759.9389625E-519.0004964
ACCGGTC1507.724945E-418.9994728
Produced by FastQC (version 0.11.5)