##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11976881952852 31.0 31.0 34.0 31.0 34.0 2 32.126495951323164 33.0 31.0 34.0 30.0 34.0 3 32.30527217393085 33.0 31.0 34.0 30.0 34.0 4 36.03806435407137 37.0 35.0 37.0 35.0 37.0 5 35.963737094202486 37.0 35.0 37.0 35.0 37.0 6 35.998621153595295 37.0 35.0 37.0 35.0 37.0 7 35.9376737707622 37.0 35.0 37.0 35.0 37.0 8 35.912268067577486 37.0 35.0 37.0 35.0 37.0 9 37.60021485855235 39.0 37.0 39.0 35.0 39.0 10-11 37.53163557704021 39.0 37.0 39.0 35.0 39.0 12-13 37.43549472987439 39.0 37.0 39.0 34.0 39.0 14-15 38.65809325702064 40.0 38.0 41.0 34.0 41.0 16-17 38.56424276105638 40.0 38.0 41.0 34.0 41.0 18-19 38.61295544245445 40.0 38.0 41.0 34.0 41.0 20-21 38.69805419868972 40.0 38.0 41.0 34.0 41.0 22-23 38.62973769576331 40.0 38.0 41.0 34.0 41.0 24-25 38.53634456929647 40.0 38.0 41.0 34.0 41.0 26-27 38.383895796297274 40.0 38.0 41.0 34.0 41.0 28-29 38.20875481221707 40.0 38.0 41.0 34.0 41.0 30-31 38.07143615639288 40.0 37.0 41.0 33.5 41.0 32-33 38.061154641020195 40.0 37.0 41.0 33.0 41.0 34-35 37.98008300030078 40.0 37.0 41.0 33.0 41.0 36-37 37.827861699226055 40.0 37.0 41.0 33.0 41.0 38-39 37.67329776074913 40.0 37.0 41.0 33.0 41.0 40-41 37.49593703878989 40.0 37.0 41.0 33.0 41.0 42-43 37.28215089258474 40.0 36.5 41.0 32.0 41.0 44-45 36.96740602229225 40.0 36.0 41.0 31.0 41.0 46-47 36.70276130433048 39.0 36.0 41.0 31.0 41.0 48-49 36.3897213954055 39.0 36.0 41.0 30.0 41.0 50-51 36.135036120601086 39.0 35.0 40.5 29.5 41.0 52-53 35.87266719994998 39.0 35.0 40.0 29.0 41.0 54-55 35.62858389563459 38.5 35.0 40.0 28.0 41.0 56-57 35.34991973798684 38.0 34.5 40.0 28.0 41.0 58-59 35.015795553409006 38.0 34.0 40.0 27.0 41.0 60-61 34.69097103560097 37.5 34.0 40.0 26.5 41.0 62-63 34.309822151453176 37.0 34.0 40.0 26.0 41.0 64-65 33.90248461869345 36.5 33.0 39.0 26.0 41.0 66-67 33.51807754959912 36.0 33.0 39.0 25.0 40.0 68-69 33.08446377536555 35.5 32.0 39.0 24.5 40.0 70-71 32.61392031151795 35.0 32.0 38.0 23.0 40.0 72-73 32.26734608182305 35.0 31.5 37.5 22.5 39.5 74-75 31.826746170440078 35.0 31.0 37.0 21.5 39.0 76-77 30.437585908383795 33.5 29.5 35.5 20.0 37.5 78-79 31.155580015675078 34.0 31.0 36.0 20.0 38.0 80-81 30.99510854507448 34.0 31.0 35.5 20.0 37.0 82-83 30.670229584933814 34.0 31.0 35.0 18.5 37.0 84-85 30.297088035947034 34.0 30.0 35.0 17.5 36.5 86-87 29.940305868883456 34.0 30.0 35.0 13.5 36.0 88-89 29.576470724578932 34.0 30.0 35.0 7.5 36.0 90-91 29.15556680936667 34.0 29.0 35.0 4.5 35.0 92-93 28.69976562845318 34.0 29.0 35.0 2.0 35.0 94-95 28.22014862217776 33.5 29.0 35.0 2.0 35.0 96-97 27.588106829871485 33.0 27.0 35.0 2.0 35.0 98-99 26.834456228903598 33.0 26.5 35.0 2.0 35.0 100-101 23.77787339624208 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4143640969298872 1101 2 0.2827990715128905 1101 3 0.26521637160652034 1101 4 0.2851031660635499 1101 5 0.2683909532008997 1101 6 0.2964621995735399 1101 7 0.3195497318112217 1101 8 0.3725023459299237 1101 9 0.37004361265262986 1101 10-11 0.40552965376958383 1101 12-13 0.39896979419218326 1101 14-15 0.5532895126892114 1101 16-17 0.4833834903586691 1101 18-19 0.5394805482106833 1101 20-21 0.5474231269032757 1101 22-23 0.5214579516000342 1101 24-25 0.46039375471147537 1101 26-27 0.5036398927879091 1101 28-29 0.43629407367220807 1101 30-31 0.4737325277674316 1101 32-33 0.5504436699142943 1101 34-35 0.5977051414940604 1101 36-37 0.5099704839832597 1101 38-39 0.5131550929869846 1101 40-41 0.4923139796195031 1101 42-43 0.5684983081063208 1101 44-45 0.6192974456217826 1101 46-47 0.5708082611549443 1101 48-49 0.4394813020334496 1101 50-51 0.4321222572774772 1101 52-53 0.2259879554865023 1101 54-55 0.30745121161739775 1101 56-57 0.484458775282512 1101 58-59 0.46431484602427986 1101 60-61 0.42082981057226476 1101 62-63 0.4476827809303714 1101 64-65 0.38853757567267877 1101 66-67 0.43540037951020594 1101 68-69 0.4242755102710234 1101 70-71 0.4097476744756037 1101 72-73 0.5642401025105173 1101 74-75 0.5667359775032743 1101 76-77 0.5259613097566067 1101 78-79 0.48848823256803087 1101 80-81 0.6599676579462859 1101 82-83 0.9441509487822515 1101 84-85 0.8974066711769595 1101 86-87 0.6911541670909891 1101 88-89 0.6250507150789097 1101 90-91 0.6988034462758641 1101 92-93 0.9118374574973629 1101 94-95 0.9750819799109713 1101 96-97 0.6883249558964692 1101 98-99 0.7106318565612 1101 100-101 0.9298881364102307 1102 1 0.2930325425225675 1102 2 0.142370553290462 1102 3 0.21958436009782645 1102 4 0.252989555461113 1102 5 0.23848842819906935 1102 6 0.26471193977266694 1102 7 0.34340292057651567 1102 8 0.3808823132115151 1102 9 0.4260131060022019 1102 10-11 0.42578789213444423 1102 12-13 0.42294050791552706 1102 14-15 0.5434478918819607 1102 16-17 0.5383126015938586 1102 18-19 0.511523751597565 1102 20-21 0.47070104951999525 1102 22-23 0.4342608309278333 1102 24-25 0.4876603868502798 1102 26-27 0.6131908329539044 1102 28-29 0.5215050562280013 1102 30-31 0.4244521913429793 1102 32-33 0.5572801539716394 1102 34-35 0.627802475058175 1102 36-37 0.6771185876557624 1102 38-39 0.6997769811853942 1102 40-41 0.7152721265910316 1102 42-43 0.7318603570411568 1102 44-45 0.7518317551643321 1102 46-47 0.5287287698325969 1102 48-49 0.6433312041408854 1102 50-51 0.6222281374990004 1102 52-53 0.704388450909768 1102 54-55 0.6720316475171231 1102 56-57 0.6206818194685226 1102 58-59 0.626092833633308 1102 60-61 0.5873810288918762 1102 62-63 0.6855910117857675 1102 64-65 0.7078357125821739 1102 66-67 0.6147943144141763 1102 68-69 0.47088341670364287 1102 70-71 0.6122716323929183 1102 72-73 0.5691729516455197 1102 74-75 0.4237190478478574 1102 76-77 0.6052142147109087 1102 78-79 0.6398799099152157 1102 80-81 0.6336355007605263 1102 82-83 0.6546197959129252 1102 84-85 0.5297348911082516 1102 86-87 0.6147841594602852 1102 88-89 0.6043462859707027 1102 90-91 0.7859322071587123 1102 92-93 0.7464943216620838 1102 94-95 0.7247925452363226 1102 96-97 0.9648123537861402 1102 98-99 1.094340405441141 1102 100-101 1.1453672846539895 1103 1 0.05082992826375232 1103 2 -0.15268860796306072 1103 3 -0.012010018816354773 1103 4 0.08441065232118206 1103 5 0.14711726110609646 1103 6 0.21222434554096736 1103 7 0.24496644608424134 1103 8 0.3183612280058199 1103 9 0.1706542434685403 1103 10-11 0.2936890017529805 1103 12-13 0.3284752594014648 1103 14-15 0.36388827365453835 1103 16-17 0.4008908852121067 1103 18-19 0.38235578148181304 1103 20-21 0.3766242288316519 1103 22-23 0.4444515025261424 1103 24-25 0.44029657427705615 1103 26-27 0.5420817051300162 1103 28-29 0.4964155016088867 1103 30-31 0.509567416241616 1103 32-33 0.47949782690099596 1103 34-35 0.2966505020963126 1103 36-37 0.3320341241554061 1103 38-39 0.43511241230500985 1103 40-41 0.3812093218139694 1103 42-43 0.342386858461154 1103 44-45 0.411512854845995 1103 46-47 0.39010343323604246 1103 48-49 0.2876944769328347 1103 50-51 0.20100261933965413 1103 52-53 0.31499913980560024 1103 54-55 0.35971727400076503 1103 56-57 0.339467280950835 1103 58-59 0.3899807002091933 1103 60-61 0.4204841884524413 1103 62-63 0.4811192613609947 1103 64-65 0.36755269545026437 1103 66-67 0.5246638774980426 1103 68-69 0.31799528023712753 1103 70-71 0.30163364773987666 1103 72-73 0.4813345915991434 1103 74-75 0.36580067136269534 1103 76-77 0.4763935754381414 1103 78-79 0.3305528883423676 1103 80-81 0.3784513004035368 1103 82-83 0.4722310797949305 1103 84-85 0.44429702422567274 1103 86-87 0.44119955181379567 1103 88-89 0.4576099691191011 1103 90-91 0.7013986272434813 1103 92-93 0.7562513280842538 1103 94-95 0.8583039143792384 1103 96-97 0.8400679831526965 1103 98-99 0.8052456738289742 1103 100-101 0.9329727106824883 1104 1 0.284992292151081 1104 2 0.15309671316912699 1104 3 0.19249895311759957 1104 4 0.09964078711320212 1104 5 0.04195989014687029 1104 6 0.19104274269175647 1104 7 0.19846234779343064 1104 8 0.22597075717099102 1104 9 0.25211395070913767 1104 10-11 0.24000480019545734 1104 12-13 0.2370181934709521 1104 14-15 0.2996188089032401 1104 16-17 0.28105336838441275 1104 18-19 0.27002980973531265 1104 20-21 0.16096125999911237 1104 22-23 0.1853538933377905 1104 24-25 0.2181577095291729 1104 26-27 0.14884942320850314 1104 28-29 0.2414826420072913 1104 30-31 0.35841205608620186 1104 32-33 0.3590954105282904 1104 34-35 0.25817470842522994 1104 36-37 0.3245604946545271 1104 38-39 0.42448752945988844 1104 40-41 0.49015867682308567 1104 42-43 0.49137080611208717 1104 44-45 0.5764831188065926 1104 46-47 0.6108683907194674 1104 48-49 0.672860538654291 1104 50-51 0.585088726048582 1104 52-53 0.5894866404446262 1104 54-55 0.5626312354546741 1104 56-57 0.709265604144953 1104 58-59 0.5799484080632524 1104 60-61 0.683621298007175 1104 62-63 0.7240137112016569 1104 64-65 0.4961078136275816 1104 66-67 0.6819602836122556 1104 68-69 0.7611457667494008 1104 70-71 0.5589313319073526 1104 72-73 0.7423880483072196 1104 74-75 0.8626161886334494 1104 76-77 0.8845271064437128 1104 78-79 0.8706995236541246 1104 80-81 1.0336065412739757 1104 82-83 0.9716781087470991 1104 84-85 0.8331225784747716 1104 86-87 0.6858406744557861 1104 88-89 0.972502271830276 1104 90-91 1.028426471764039 1104 92-93 1.0727786869445737 1104 94-95 0.911478172682326 1104 96-97 1.0996601989562507 1104 98-99 1.0708042683111962 1104 100-101 0.9936176163621546 1105 1 0.18149890880003028 1105 2 0.0223794470253722 1105 3 0.11258845675190798 1105 4 0.1073281696752062 1105 5 0.16851374946869413 1105 6 0.11900602513807712 1105 7 0.16185429146331387 1105 8 0.20147678537617253 1105 9 0.20855406652557207 1105 10-11 0.21819560308686192 1105 12-13 0.3232231156693288 1105 14-15 0.32999178529193784 1105 16-17 0.24804618669571 1105 18-19 0.25912521118101495 1105 20-21 0.1740925832620306 1105 22-23 0.33119098803042846 1105 24-25 0.29973239842296096 1105 26-27 0.3259969086144281 1105 28-29 0.25367696887443714 1105 30-31 0.2609029953895643 1105 32-33 0.2489377343462138 1105 34-35 0.29281390212353386 1105 36-37 0.3039716496760221 1105 38-39 0.2897894913849015 1105 40-41 0.09507552570671152 1105 42-43 0.3012629303272121 1105 44-45 0.47269258807043 1105 46-47 0.29777493180689163 1105 48-49 0.27078961780666333 1105 50-51 0.25659333663923434 1105 52-53 0.3415746127877739 1105 54-55 0.3064421276825371 1105 56-57 0.3567447411386766 1105 58-59 0.2424119646708789 1105 60-61 0.2712668208193989 1105 62-63 0.15687637310728064 1105 64-65 0.249877113580105 1105 66-67 0.22164053463701805 1105 68-69 0.097272536259986 1105 70-71 0.07070605824756626 1105 72-73 0.23040393963235317 1105 74-75 0.19847046855882766 1105 76-77 0.14846210681148975 1105 78-79 0.28540861176689347 1105 80-81 0.41347750157408214 1105 82-83 0.3756372319658894 1105 84-85 0.4356693530673752 1105 86-87 0.2111107400538934 1105 88-89 0.31382849368695176 1105 90-91 0.4301943942232036 1105 92-93 0.41247665815095047 1105 94-95 0.4230769509928187 1105 96-97 0.6346065703311368 1105 98-99 0.664419486269427 1105 100-101 0.6117514639150983 1106 1 0.4272322215029547 1106 2 0.3044333267697539 1106 3 0.3246880672301913 1106 4 0.22232733463495435 1106 5 0.21782901646053432 1106 6 0.295191536698475 1106 7 0.3037307005937393 1106 8 0.352687839344334 1106 9 0.45645418588721043 1106 10-11 0.4388390261624622 1106 12-13 0.45291008609416394 1106 14-15 0.6365894014976092 1106 16-17 0.6035762407768459 1106 18-19 0.6583002405302167 1106 20-21 0.5709393239034384 1106 22-23 0.5715106610876077 1106 24-25 0.5829264765139612 1106 26-27 0.5429053685545995 1106 28-29 0.6733651304596364 1106 30-31 0.7462673799403419 1106 32-33 0.7623389024201757 1106 34-35 0.5958183892368965 1106 36-37 0.5828800064641655 1106 38-39 0.718784974799533 1106 40-41 0.6554845480710583 1106 42-43 0.7449201241989982 1106 44-45 0.7812169368618385 1106 46-47 0.5693297153595367 1106 48-49 0.4953540460742474 1106 50-51 0.6056106907405123 1106 52-53 0.5649834927040729 1106 54-55 0.5893184606769424 1106 56-57 0.5970076191785907 1106 58-59 0.49314047589234633 1106 60-61 0.5522118162266807 1106 62-63 0.6248031523570248 1106 64-65 0.5991047627453128 1106 66-67 0.7522149809316474 1106 68-69 0.8273347221988985 1106 70-71 0.7733562058532115 1106 72-73 0.8948574426262184 1106 74-75 0.6967353040313391 1106 76-77 0.7838027909550576 1106 78-79 0.8676969167825348 1106 80-81 0.8016149565678674 1106 82-83 0.8986984649765049 1106 84-85 0.8795874970184911 1106 86-87 0.7788543016479359 1106 88-89 0.9251525605294653 1106 90-91 1.2485280099966225 1106 92-93 1.201360982971785 1106 94-95 1.0856196854058133 1106 96-97 1.2267059843293566 1106 98-99 1.3757073885009525 1106 100-101 1.5209147413007322 1107 1 0.15339963368430887 1107 2 0.0707983918838977 1107 3 0.009571312204329274 1107 4 0.07931063588323184 1107 5 0.06089935053989137 1107 6 0.06833557946436031 1107 7 0.04688345142941586 1107 8 0.0032045856559861363 1107 9 0.07000919554340612 1107 10-11 0.12396175839759849 1107 12-13 0.1351517604982675 1107 14-15 0.08592150665968035 1107 16-17 0.1611576618430135 1107 18-19 0.1627199388100209 1107 20-21 0.12223132775977774 1107 22-23 0.12113469216909323 1107 24-25 0.1290103611845126 1107 26-27 0.1535035313298465 1107 28-29 0.1306026165215073 1107 30-31 0.21880412842440222 1107 32-33 0.1886621270693425 1107 34-35 0.3161089282473952 1107 36-37 0.22824248370444167 1107 38-39 0.20360369168785297 1107 40-41 0.15325644205475442 1107 42-43 0.1430527372397563 1107 44-45 0.2004248891276177 1107 46-47 0.04523830946959606 1107 48-49 -0.013979360082025494 1107 50-51 0.0259938335375125 1107 52-53 0.05018514373937677 1107 54-55 0.03285822660844673 1107 56-57 0.1333530711912303 1107 58-59 0.16712528617118494 1107 60-61 0.232769715685599 1107 62-63 0.0835867964050081 1107 64-65 0.012816014442897483 1107 66-67 0.013788613215453438 1107 68-69 0.007532240122849032 1107 70-71 -0.11207560456001886 1107 72-73 -0.0057910907175866555 1107 74-75 0.0734963255081702 1107 76-77 0.22509696108950905 1107 78-79 0.16443590113562578 1107 80-81 0.21792236677318755 1107 82-83 0.3392103509701343 1107 84-85 0.282378375085262 1107 86-87 0.3390911010057849 1107 88-89 0.24505860051785788 1107 90-91 0.4077109426707821 1107 92-93 0.40317218784584696 1107 94-95 0.3997869294398022 1107 96-97 0.5928131212031538 1107 98-99 0.7168204013274035 1107 100-101 0.7865234501069303 1108 1 -0.03806311992261158 1108 2 -0.17683170226337452 1108 3 -0.04907803960560386 1108 4 -0.007120113821414975 1108 5 -0.010309797474896243 1108 6 0.016343135076894555 1108 7 -0.012501301276408583 1108 8 -0.006153490482077473 1108 9 0.01254632597453309 1108 10-11 -0.010612616032894095 1108 12-13 0.033446614500583394 1108 14-15 0.04436444087999547 1108 16-17 0.22147316877492784 1108 18-19 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-0.8332987380135357 2316 74-75 -0.7635392050053156 2316 76-77 -1.2194111221524686 2316 78-79 -0.995671206608705 2316 80-81 -1.0494229043068088 2316 82-83 -0.8284792992333365 2316 84-85 -0.8014582418276213 2316 86-87 -0.8012962890272846 2316 88-89 -1.0299549429937507 2316 90-91 -1.0691583811386174 2316 92-93 -1.2719641136901743 2316 94-95 -0.85151954061946 2316 96-97 -0.8079717640554875 2316 98-99 -0.6112226022860234 2316 100-101 -0.5889938937961183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 15.0 5 35.0 6 78.0 7 196.0 8 496.0 9 892.0 10 1657.0 11 2581.0 12 3799.0 13 5320.0 14 6931.0 15 9183.0 16 12039.0 17 16442.0 18 14954.0 19 9897.0 20 8927.0 21 9024.0 22 9721.0 23 10521.0 24 12576.0 25 14859.0 26 18572.0 27 22877.0 28 29016.0 29 35975.0 30 45393.0 31 57136.0 32 72860.0 33 95383.0 34 127977.0 35 181855.0 36 276804.0 37 410129.0 38 303939.0 39 27105.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.591434486327955 19.423682394548432 15.170577072371257 22.81430604675235 2 15.709350282653379 20.618252718588064 39.621119951010705 24.051277047747856 3 19.325274577184825 24.478997372144732 29.933831952024796 26.261896098645643 4 13.352490638309112 17.341511572812582 36.807414103420335 32.498583685457966 5 14.099244154894883 36.916473240323384 34.10175609198649 14.882526512795247 6 31.881087036673687 36.33091702548335 17.33278639393005 14.45520954391291 7 28.309504455651552 32.664141114678316 20.808798321665055 18.217556108005077 8 26.396068508937702 34.830156093175084 19.606570596612503 19.167204801274707 9 26.26605375021426 16.76311763748306 20.29426889337604 36.67655971892663 10-11 24.873212598828413 26.772469945517198 27.222104682987702 21.13221277266669 12-13 25.920716229714003 23.935360796567842 27.892865247668798 22.251057726049357 14-15 22.872355908394574 25.248602017923925 26.19875548061799 25.680286593063506 16-17 22.269609211858292 28.482287914772414 26.801636935403362 22.446465937965925 18-19 22.104099130324165 26.875592262504753 29.386758331024478 21.633550276146604 20-21 22.78750465935311 26.009068320738145 29.62146512432084 21.581961895587906 22-23 22.520960298063684 25.867465801051548 29.565393866020113 22.046180034864655 24-25 22.346854012351834 26.169567394692173 29.62664188465476 21.856936708301237 26-27 22.25364837104202 26.360474637265156 29.4032319880462 21.98264500364662 28-29 22.143185578052492 26.30230330485894 29.59721677931904 21.957294337769532 30-31 22.279746376166994 26.20211734172636 29.50374500033156 22.014391281775087 32-33 22.2360964261063 26.208607714207094 29.443393472751563 22.111902386935046 34-35 22.522767278510745 26.260242209506128 29.32444877837369 21.892541733609434 36-37 22.517437696906022 26.043195949338703 29.2355767559186 22.20378959783667 38-39 22.353710426301546 26.281304738399808 29.320659651706443 22.044325183592207 40-41 22.54141308507943 26.143825906020734 29.339223433903477 21.975537574996363 42-43 22.431203775251877 26.24108925794868 29.05479360121867 22.27291336558077 44-45 22.533156098328774 26.413675556446766 28.87273038933485 22.180437955889616 46-47 22.690106362105507 26.627665790557426 28.3247860575731 22.357441789763968 48-49 22.78464432244907 26.727112144139852 28.158463063098516 22.329780470312567 50-51 22.68538981830256 26.82700040750938 28.03955644004976 22.44805333413829 52-53 22.852862880936076 26.8961197487751 27.879247532747343 22.371769837541482 54-55 22.821862168131755 26.848766051837547 27.859130596496996 22.4702411835337 56-57 22.74267565741404 27.160931377318427 27.752765048312376 22.343627916955164 58-59 22.81040734689521 26.864524774533177 27.902661292800424 22.422406585771185 60-61 22.8144487079385 26.801985062707683 28.090825809480663 22.29274041987316 62-63 22.72638577297871 26.827429664279467 28.134611632116446 22.311572930625374 64-65 22.75953388688488 26.750053364324174 28.002363123203672 22.488049625587276 66-67 22.660926323063585 27.09450086474392 27.7565144176542 22.488058394538303 68-69 22.62758102474485 26.899282762695037 28.00028460942208 22.472851603138032 70-71 22.69868559463576 26.80749266847548 27.90286191561059 22.590959821278172 72-73 22.7180099327637 26.861126466069074 27.766648240717107 22.654215360450113 74-75 22.67001731383317 27.07019433322337 27.515977024133715 22.743811328809745 76-77 22.752673623838376 27.065293559585157 27.595220897388906 22.586811919187564 78-79 22.7098986669139 27.061712436980134 27.570048874253505 22.658340021852457 80-81 22.62235449557189 27.210752522396596 27.49044381350464 22.676449168526876 82-83 22.69571193704013 27.172519742338896 27.565479880333125 22.566288440287845 84-85 22.718123591059975 27.134823768622805 27.29901817566322 22.848034464654003 86-87 22.734704152346126 26.992352076173066 27.517890794461238 22.755052977019574 88-89 22.812047628403796 27.020486444922216 27.407166037742968 22.760299888931023 90-91 22.68265908712973 27.079478678644364 27.466505100205076 22.771357134020835 92-93 22.688410898384735 26.870875723786558 27.484726500047973 22.955986877780738 94-95 22.59850719799652 26.8489555398996 27.52450046330011 23.028036798803775 96-97 22.716752863719535 26.85491215393269 27.45516059765566 22.973174384692115 98-99 22.465568450728522 26.847471605763477 27.343501533585247 23.343458409922754 100-101 22.347650056342804 27.044087536314226 27.31072200452629 23.297540402816683 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 592.0 1 428.0 2 307.0 3 352.5 4 289.0 5 269.0 6 491.0 7 1178.5 8 1979.0 9 2328.0 10 2096.5 11 1750.5 12 1756.5 13 1942.0 14 2102.0 15 2201.0 16 2430.5 17 2666.0 18 2831.5 19 3056.5 20 3409.0 21 3906.0 22 4643.5 23 5664.0 24 6916.5 25 8589.5 26 10950.0 27 13657.5 28 16154.5 29 18601.5 30 21357.0 31 24427.5 32 27674.0 33 30675.0 34 34111.5 35 37947.5 36 42451.0 37 46611.5 38 48907.0 39 50298.5 40 52261.0 41 55281.0 42 57407.0 43 59702.5 44 62770.0 45 65005.0 46 65814.0 47 66827.0 48 69655.0 49 70372.5 50 68878.5 51 67970.5 52 66447.5 53 65577.0 54 64888.5 55 61142.5 56 56335.0 57 51476.0 58 46691.0 59 41491.5 60 35035.0 61 28824.5 62 22872.0 63 17255.0 64 12696.0 65 9490.0 66 6974.5 67 5437.5 68 4102.0 69 2820.5 70 2031.5 71 1313.0 72 875.0 73 602.0 74 427.5 75 298.5 76 178.5 77 89.0 78 54.5 79 35.5 80 24.5 81 13.0 82 7.0 83 6.5 84 4.5 85 3.0 86 3.0 87 2.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015847570093155683 2 0.0040427474727437965 3 0.002641261682192614 4 3.773230974560877E-4 5 0.0017249055883706865 6 1.078065992731679E-4 7 1.078065992731679E-4 8 0.0 9 0.0 10-11 8.085494945487594E-5 12-13 8.085494945487592E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0018596638374621464 22-23 0.0 24-25 0.003503714476377957 26-27 0.0016710022887341028 28-29 0.003072488079285285 30-31 0.01622489319061177 32-33 6.737912454572994E-4 34-35 0.002398696833827986 36-37 0.003746279324742585 38-39 0.002344793534191402 40-41 0.0045278771694730515 42-43 0.002129180335645066 44-45 5.390329963658396E-4 46-47 1.078065992731679E-4 48-49 1.8866154872804384E-4 50-51 1.078065992731679E-4 52-53 0.0012667275414597228 54-55 0.0027760199312840735 56-57 4.042747472743796E-4 58-59 0.0012397758916414309 60-61 9.972110432768032E-4 62-63 0.0 64-65 1.078065992731679E-4 66-67 2.6951649818291976E-5 68-69 0.0 70-71 2.6951649818291976E-5 72-73 2.6951649818291976E-5 74-75 5.390329963658396E-4 76-77 7.546461949121754E-4 78-79 2.695164981829198E-4 80-81 0.005013006866202308 82-83 0.001293679191278015 84-85 0.0030185847796487014 86-87 0.0016170989890975185 88-89 0.0011050176425499712 90-91 1.8866154872804384E-4 92-93 0.0 94-95 2.695164981829198E-4 96-97 0.0013745341407328908 98-99 0.002371745184009694 100-101 6.737912454572994E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1855174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.489754176370084 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10493857688667 48.64143227229617 2 12.538115563839922 15.419312877108732 3 3.6584528958923723 6.748721077027538 4 1.5379992360165808 3.782847797844182 5 0.8727007156201227 2.6831076236511806 6 0.4950633673909102 1.8264794857555837 7 0.345400214402644 1.4867001993258873 8 0.2342639146631758 1.1523864420026257 9 0.17243064782235637 0.9542446332362042 >10 0.9512369490865829 10.639164637377691 >50 0.06188508542237125 2.5848160860281264 >100 0.02654624952894389 2.7266959892143983 >500 5.272273239947526E-4 0.1982426653179793 >1k 2.636136619973756E-4 0.38243706326537324 >5k 1.7574244133158374E-4 0.7734111505482555 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7891 0.425350937432284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6423 0.34622089356577873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4268 0.2300592828489403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.390329963658395E-5 7 0.0 0.0 0.0 0.0 5.390329963658395E-5 8 0.0 0.0 0.0 0.0 5.390329963658395E-5 9 0.0 0.0 0.0 0.0 5.390329963658395E-5 10-11 0.0 0.0 0.0 0.0 5.390329963658395E-5 12-13 0.0 0.0 0.0 0.0 2.156131985463358E-4 14-15 0.0 0.0 0.0 0.0 2.156131985463358E-4 16-17 0.0 0.0 0.0 8.085494945487594E-5 2.156131985463358E-4 18-19 2.6951649818291976E-5 0.0 0.0 1.3475824909145987E-4 2.156131985463358E-4 20-21 5.390329963658395E-5 0.0 0.0 2.156131985463358E-4 2.6951649818291975E-4 22-23 5.390329963658395E-5 0.0 0.0 3.2341979781950374E-4 2.6951649818291975E-4 24-25 5.390329963658395E-5 0.0 0.0 5.929362960024235E-4 2.6951649818291975E-4 26-27 5.390329963658395E-5 0.0 0.0 0.001024162693095095 3.2341979781950374E-4 28-29 1.078065992731679E-4 0.0 0.0 0.0018327121876438547 3.2341979781950374E-4 30-31 1.078065992731679E-4 0.0 0.0 0.0030994397291035773 3.503714476377957E-4 32-33 1.078065992731679E-4 0.0 0.0 0.00409665077238038 3.773230974560877E-4 34-35 1.078065992731679E-4 0.0 0.0 0.006010217909479111 3.773230974560877E-4 36-37 1.6170989890975187E-4 0.0 0.0 0.008355011443670513 3.773230974560877E-4 38-39 1.6170989890975187E-4 0.0 0.0 0.012289952317141143 3.773230974560877E-4 40-41 1.6170989890975187E-4 0.0 0.0 0.015389392046244718 3.773230974560877E-4 42-43 1.6170989890975187E-4 0.0 0.0 0.019000913121895842 3.773230974560877E-4 44-45 1.6170989890975187E-4 0.0 0.0 0.020402398912447026 3.773230974560877E-4 46-47 1.6170989890975187E-4 0.0 0.0 0.022935853995366472 3.773230974560877E-4 48-49 1.6170989890975187E-4 0.0 0.0 0.026682133320109058 4.042747472743797E-4 50-51 1.6170989890975187E-4 0.0 0.0 0.030617074193579688 4.851296967292556E-4 52-53 1.6170989890975187E-4 0.0 0.0 0.03937636038452458 5.929362960024235E-4 54-55 1.6170989890975187E-4 0.0 0.0 0.04436241560090859 5.929362960024235E-4 56-57 1.6170989890975187E-4 0.0 0.0 0.058538983405330175 5.929362960024235E-4 58-59 1.6170989890975187E-4 0.0 0.0 0.08403524413343438 5.929362960024235E-4 60-61 1.6170989890975187E-4 0.0 0.0 0.10424898149715336 5.929362960024235E-4 62-63 1.6170989890975187E-4 0.0 0.0 0.11680845031247743 7.007428952755914E-4 64-65 1.6170989890975187E-4 0.0 0.0 0.14160396814530604 7.007428952755914E-4 66-67 1.6170989890975187E-4 0.0 0.0 0.1904134059662328 7.007428952755914E-4 68-69 1.6170989890975187E-4 0.0 0.0 0.2607572119919749 7.007428952755914E-4 70-71 1.6170989890975187E-4 0.0 0.0 0.32029340644058185 7.007428952755914E-4 72-73 1.6170989890975187E-4 0.0 0.0 0.3888045002786801 7.546461949121754E-4 74-75 1.6170989890975187E-4 0.0 0.0 0.47445684340121197 8.355011443670513E-4 76-77 1.6170989890975187E-4 0.0 0.0 0.5280636748897947 9.433077436402193E-4 78-79 1.6170989890975187E-4 0.0 0.0 0.593071054251515 9.702593934585112E-4 80-81 2.156131985463358E-4 0.0 0.0 0.6831704195940651 9.972110432768032E-4 82-83 2.156131985463358E-4 0.0 0.0 0.7640792723485775 0.001078065992731679 84-85 2.6951649818291975E-4 0.0 0.0 0.8779230411810428 0.001078065992731679 86-87 3.2341979781950374E-4 0.0 0.0 1.0682286405480026 0.001185872592004847 88-89 3.2341979781950374E-4 0.0 0.0 1.2919812373394626 0.001293679191278015 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 50.018715 1 GTATCAA 7100 0.0 38.40655 1 ATCAACG 9090 0.0 29.786255 3 TCAACGC 9250 0.0 29.322388 4 TATCAAC 9245 0.0 29.18489 2 CAACGCA 9395 0.0 28.920393 5 AACGCAG 9700 0.0 28.010284 6 ACGCAGA 11110 0.0 24.283754 7 CGCAGAG 11275 0.0 23.801998 8 GTACATG 7175 0.0 23.769733 1 ACATGGG 6925 0.0 23.459097 3 TACATGG 7225 0.0 22.945854 2 GAGTACT 7305 0.0 21.87966 12-13 GCAGAGT 12395 0.0 21.383028 9 GTACTTT 7715 0.0 20.748524 14-15 AGTACTT 7630 0.0 20.13842 12-13 AGAGTAC 11090 0.0 19.680473 10-11 CAGAGTA 11865 0.0 19.215647 10-11 TACGGGT 175 9.9389625E-5 19.000496 4 ACCGGTC 150 7.724945E-4 18.999472 8 >>END_MODULE