FastQCFastQC Report
Thu 26 May 2016
SRR936866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796969
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65430.36411312604724955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.28147397089209664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.17690900622103106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.048.4611971
GTATCAA64100.041.8962061
TATCAAC82150.032.4014742
TCAACGC82700.032.0710334
ATCAACG82700.032.0135573
CAACGCA84350.031.3336015
AACGCAG86550.030.7533996
GCGAATA700.00836544927.1610329
ACGCAGA97450.027.0194137
CGCAGAG98150.026.4415348
GCAGAGT107000.023.543799
GAGTACT60150.022.47803512-13
TACATGG67500.022.4631792
GTACATG68000.022.3679071
ATAGCGC1100.00286729821.6047636
ACATGGG68450.021.1098143
GTACTTT64200.020.5785814-15
CAGAGTA100650.020.25748310-11
AGAGTAC96600.020.24578710-11
GTGGTAT20450.019.9889221
Produced by FastQC (version 0.11.5)