FastQCFastQC Report
Thu 26 May 2016
SRR936866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796969
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77240.42983490533225666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61230.34074043570033763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.231222686646236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.051.1763571
GTATCAA69850.043.192471
TATCAAC90450.033.5027052
ATCAACG89950.033.4230583
TCAACGC91400.032.839034
CAACGCA93050.032.3077555
AACGCAG95600.031.5941436
ACGCAGA109550.027.3975727
CGCAGAG110750.027.057838
GTGGTAT18800.024.5140251
TGGTATC18050.024.4722292
GCAGAGT120600.024.2572049
GTACATG65650.022.14561
GAGTACT73000.021.7948312-13
TACATGG66650.021.1652832
AGAGTAC107250.020.81171810-11
AGTACTT76700.020.43384612-13
ACATGGG64950.020.3286843
CAGAGTA114800.020.2496810-11
GTACTTT78150.019.99505414-15
Produced by FastQC (version 0.11.5)