##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1796969 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18977233330124 33.0 31.0 34.0 31.0 34.0 2 32.27312268603409 33.0 31.0 34.0 30.0 34.0 3 32.40981619605013 34.0 31.0 34.0 31.0 34.0 4 36.08584177022531 37.0 35.0 37.0 35.0 37.0 5 35.9950071481478 37.0 35.0 37.0 35.0 37.0 6 36.02373496704729 37.0 35.0 37.0 35.0 37.0 7 35.97516262105802 37.0 35.0 37.0 35.0 37.0 8 35.95796532939633 37.0 35.0 37.0 35.0 37.0 9 37.65656502699824 39.0 37.0 39.0 35.0 39.0 10-11 37.55448118470603 39.0 37.0 39.0 35.0 39.0 12-13 37.48166607214704 39.0 37.0 39.0 35.0 39.0 14-15 38.72334636824564 40.0 38.0 41.0 35.0 41.0 16-17 38.63392913288988 40.0 38.0 41.0 34.0 41.0 18-19 38.65845459771426 40.0 38.0 41.0 34.0 41.0 20-21 38.752060553075765 40.0 38.0 41.0 34.0 41.0 22-23 38.693211457738 40.0 38.0 41.0 34.0 41.0 24-25 38.61260266593358 40.0 38.0 41.0 34.0 41.0 26-27 38.44842481979377 40.0 38.0 41.0 34.0 41.0 28-29 38.29579697813374 40.0 38.0 41.0 34.0 41.0 30-31 38.24814896639842 40.0 38.0 41.0 34.0 41.0 32-33 38.17289363366869 40.0 38.0 41.0 34.0 41.0 34-35 38.06442376023181 40.0 37.5 41.0 33.0 41.0 36-37 37.899643232576636 40.0 37.0 41.0 33.0 41.0 38-39 37.76376303653541 40.0 37.0 41.0 33.0 41.0 40-41 37.54090749478706 40.0 37.0 41.0 33.0 41.0 42-43 37.32788378653166 40.0 37.0 41.0 32.0 41.0 44-45 37.10382928141777 40.0 36.0 41.0 31.5 41.0 46-47 36.77273954086019 39.5 36.0 41.0 31.0 41.0 48-49 36.4181608029966 39.0 36.0 41.0 30.0 41.0 50-51 36.17954010336294 39.0 35.0 41.0 30.0 41.0 52-53 35.95333698021501 39.0 35.0 40.0 29.0 41.0 54-55 35.68319709466329 39.0 35.0 40.0 28.0 41.0 56-57 35.42856248493992 38.0 35.0 40.0 28.0 41.0 58-59 35.07616241571223 38.0 34.0 40.0 27.5 41.0 60-61 34.745523434182786 38.0 34.0 40.0 26.5 41.0 62-63 34.39881099785249 37.0 34.0 40.0 26.0 41.0 64-65 33.98644328310617 36.5 33.0 39.0 26.0 41.0 66-67 33.54877157034985 36.0 33.0 39.0 25.0 40.0 68-69 33.1094220323222 35.5 32.0 39.0 24.5 40.0 70-71 32.717871593778185 35.0 32.0 38.0 24.0 40.0 72-73 32.323820833859685 35.0 32.0 37.5 22.5 39.5 74-75 31.848452310529563 35.0 31.0 37.0 21.5 39.0 76-77 30.39911901652171 33.5 29.5 35.5 20.0 37.5 78-79 31.10480843019551 34.0 31.0 36.0 20.0 38.0 80-81 30.838220915330204 34.0 31.0 35.5 18.0 37.0 82-83 30.62840316110072 34.0 31.0 35.0 18.0 37.0 84-85 30.29275407644762 34.0 30.0 35.0 17.5 36.5 86-87 29.9248225762381 34.0 30.0 35.0 13.0 36.0 88-89 29.546261232108066 34.0 30.0 35.0 7.0 36.0 90-91 29.1025053854574 34.0 29.0 35.0 4.0 35.0 92-93 28.661513359440256 34.0 29.0 35.0 2.0 35.0 94-95 28.172849948997452 33.5 29.0 35.0 2.0 35.0 96-97 27.539782544940955 33.0 27.0 35.0 2.0 35.0 98-99 26.743980558373572 33.0 25.5 34.5 2.0 35.0 100-101 23.47891310311975 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35771455523079254 1101 2 0.27756095343642073 1101 3 0.24563278802017408 1101 4 0.17819977088315397 1101 5 0.28549955429593155 1101 6 0.26507106860206164 1101 7 0.17731402469263458 1101 8 0.2533303123770452 1101 9 0.33542723207494163 1101 10-11 0.29704017431149765 1101 12-13 0.2705688854765711 1101 14-15 0.3824014784640468 1101 16-17 0.32320311670760304 1101 18-19 0.36678833992625215 1101 20-21 0.29405712931723116 1101 22-23 0.35152387251007866 1101 24-25 0.3246884031170154 1101 26-27 0.2809157501660309 1101 28-29 0.29054365294274476 1101 30-31 0.28174330826317373 1101 32-33 0.3477407110978348 1101 34-35 0.18667114463358558 1101 36-37 0.3402945790628351 1101 38-39 0.27332959673019985 1101 40-41 0.37038291708820736 1101 42-43 0.3513150179981679 1101 44-45 0.26199457559931005 1101 46-47 0.24804792487034177 1101 48-49 0.29732093996098996 1101 50-51 0.16744793773802513 1101 52-53 0.22925091115840246 1101 54-55 0.13054163463829127 1101 56-57 0.2855726611715639 1101 58-59 0.44707797201483146 1101 60-61 0.27510144634812406 1101 62-63 0.16799676046706935 1101 64-65 0.261975466605584 1101 66-67 0.14323713836245133 1101 68-69 0.17967455988201664 1101 70-71 0.17908070588085678 1101 72-73 0.2512058974846809 1101 74-75 0.17497049440423496 1101 76-77 0.12853421938241993 1101 78-79 0.3176960072850292 1101 80-81 0.5679579227083735 1101 82-83 0.49151950421229174 1101 84-85 0.46182024927108856 1101 86-87 0.36568395226811745 1101 88-89 0.22911023199462832 1101 90-91 0.14876882078070608 1101 92-93 0.36381751579782673 1101 94-95 0.43289058175967554 1101 96-97 0.3376905501047993 1101 98-99 0.5145478917221418 1101 100-101 0.5977722538965722 1102 1 0.31357621223313004 1102 2 0.2852645580981985 1102 3 0.24553653165015277 1102 4 0.1946271120253229 1102 5 0.24045157312627907 1102 6 0.29385358027430897 1102 7 0.22076557384760775 1102 8 0.2020826162624303 1102 9 0.28652992462249927 1102 10-11 0.2972586855566277 1102 12-13 0.3220897206475897 1102 14-15 0.3757407543128295 1102 16-17 0.4009493248720588 1102 18-19 0.4721367585826428 1102 20-21 0.37634982605859335 1102 22-23 0.46630355839243265 1102 24-25 0.40038660999393727 1102 26-27 0.4316365122936432 1102 28-29 0.35961703836593983 1102 30-31 0.4718736257699163 1102 32-33 0.4328981954948645 1102 34-35 0.3319896340585373 1102 36-37 0.43685674102423633 1102 38-39 0.3496497559400922 1102 40-41 0.5579738925118889 1102 42-43 0.44331537083502326 1102 44-45 0.35495888491342953 1102 46-47 0.4927839239789691 1102 48-49 0.6020045397016318 1102 50-51 0.6248905514543992 1102 52-53 0.43917769751104174 1102 54-55 0.4644815997569651 1102 56-57 0.4838032152956586 1102 58-59 0.46372784798030153 1102 60-61 0.45464217037553567 1102 62-63 0.5090047596499758 1102 64-65 0.47915924044335156 1102 66-67 0.48596428869416286 1102 68-69 0.3624307418777448 1102 70-71 0.13873690660170013 1102 72-73 0.1970270246746324 1102 74-75 0.27520355243196093 1102 76-77 0.3993908236991004 1102 78-79 0.3504423624648467 1102 80-81 0.40358206479639236 1102 82-83 0.3464510956457829 1102 84-85 0.22701165552713576 1102 86-87 0.25489751296456475 1102 88-89 0.17446292183401013 1102 90-91 0.08459414177712787 1102 92-93 0.4463596743856115 1102 94-95 0.6093037475264538 1102 96-97 0.5158680548982737 1102 98-99 0.4888568808600269 1102 100-101 0.9102286328928741 1103 1 0.27888395271023114 1103 2 0.20003759534537835 1103 3 0.17554389664307024 1103 4 0.1446406323021847 1103 5 0.22134380465983128 1103 6 0.29209149682336033 1103 7 0.26697052701289437 1103 8 0.2786951464755063 1103 9 0.3413647281871093 1103 10-11 0.25691993146199366 1103 12-13 0.3130941408293424 1103 14-15 0.4121205282158513 1103 16-17 0.4212632443528861 1103 18-19 0.5253891560598092 1103 20-21 0.4287527587726814 1103 22-23 0.4361233947733538 1103 24-25 0.382080570225348 1103 26-27 0.46100056709583015 1103 28-29 0.38518311101015 1103 30-31 0.34652702042679806 1103 32-33 0.4639542121366631 1103 34-35 0.537446844754264 1103 36-37 0.5786562780140585 1103 38-39 0.5438873605739829 1103 40-41 0.49155138148811517 1103 42-43 0.4759893732402105 1103 44-45 0.6177364360354929 1103 46-47 0.5499612044582918 1103 48-49 0.5130467514291652 1103 50-51 0.499663286274334 1103 52-53 0.5624335521467145 1103 54-55 0.5598686354718438 1103 56-57 0.4571513695406608 1103 58-59 0.4300438275243508 1103 60-61 0.5469809173542259 1103 62-63 0.655326473451801 1103 64-65 0.5564326631370236 1103 66-67 0.5154696244595698 1103 68-69 0.6294115869662136 1103 70-71 0.598954677938238 1103 72-73 0.5139271928478948 1103 74-75 0.4519472234006088 1103 76-77 0.6305165468560148 1103 78-79 0.7018740886246846 1103 80-81 0.9486358671963622 1103 82-83 0.777939464649716 1103 84-85 0.6401692272379726 1103 86-87 0.5635249617357623 1103 88-89 0.5424566906467625 1103 90-91 0.5468614506964791 1103 92-93 0.47394578313133096 1103 94-95 0.6937682674736045 1103 96-97 0.8846934986361283 1103 98-99 0.898431430955025 1103 100-101 1.1159400900229208 1104 1 0.2543498082530036 1104 2 0.15954889047513632 1104 3 0.20906139773419596 1104 4 0.19084166904082167 1104 5 0.09472813081472964 1104 6 0.17827926508123682 1104 7 0.14674548797765397 1104 8 0.2151698149968766 1104 9 0.23030801097495868 1104 10-11 0.24817726296862475 1104 12-13 0.24090301519071033 1104 14-15 0.31552741778987325 1104 16-17 0.30917113052169043 1104 18-19 0.30750395584228585 1104 20-21 0.31042755145995926 1104 22-23 0.37328745247783246 1104 24-25 0.3192119526913402 1104 26-27 0.447679718426528 1104 28-29 0.3724674836105635 1104 30-31 0.4014745183204127 1104 32-33 0.4294336979217661 1104 34-35 0.33502257545428193 1104 36-37 0.39930685368174323 1104 38-39 0.4341976061284001 1104 40-41 0.5586616534161166 1104 42-43 0.3771141016272921 1104 44-45 0.45702823525082437 1104 46-47 0.5887241024251963 1104 48-49 0.6925455482802079 1104 50-51 0.6031844224857323 1104 52-53 0.6393296203819574 1104 54-55 0.5945385222054682 1104 56-57 0.47218052052127035 1104 58-59 0.48458857392949284 1104 60-61 0.5008481485245326 1104 62-63 0.37999820400245454 1104 64-65 0.43290071492260296 1104 66-67 0.12990910408305467 1104 68-69 0.3462915859859521 1104 70-71 0.43195095062710465 1104 72-73 0.4248711221386827 1104 74-75 0.5458352908703077 1104 76-77 0.6596396659875978 1104 78-79 0.6562985493082003 1104 80-81 0.7422805041394547 1104 82-83 0.6207488116350781 1104 84-85 0.43936748774831 1104 86-87 0.5802930480637407 1104 88-89 0.6348858962416948 1104 90-91 0.7625315906631975 1104 92-93 0.8184641617860002 1104 94-95 0.7611138302929206 1104 96-97 0.7937262182195717 1104 98-99 1.1544713904377275 1104 100-101 0.9534080566194909 1105 1 0.13614934795129585 1105 2 0.15564597061918306 1105 3 0.10833380184900676 1105 4 0.1319752351849317 1105 5 0.14450420878930714 1105 6 0.1340965015372646 1105 7 0.12256787972092553 1105 8 0.09780780647859899 1105 9 0.19966964518953034 1105 10-11 0.18435216613477223 1105 12-13 0.115387127663233 1105 14-15 0.2758420395737815 1105 16-17 0.19095904498984595 1105 18-19 0.2549135675251577 1105 20-21 0.25613586094483054 1105 22-23 0.028697116976552195 1105 24-25 0.11552385560660383 1105 26-27 0.23792640868045112 1105 28-29 0.1978985787187355 1105 30-31 0.1242089044881709 1105 32-33 0.04858699653058807 1105 34-35 0.0573238141584298 1105 36-37 0.021454438418182065 1105 38-39 0.22131050749306524 1105 40-41 0.3260194137021486 1105 42-43 0.318447593025482 1105 44-45 0.21230594621142984 1105 46-47 0.10435903230926868 1105 48-49 0.23354673032212503 1105 50-51 0.1356163747034529 1105 52-53 0.24008477236820625 1105 54-55 0.2346671804931617 1105 56-57 0.0793605496962968 1105 58-59 0.2775416464634688 1105 60-61 0.353469221708373 1105 62-63 0.15250106151273002 1105 64-65 0.2603167104355535 1105 66-67 0.2514715522359694 1105 68-69 0.2920293173495594 1105 70-71 0.2900378912741317 1105 72-73 0.3019450775471668 1105 74-75 0.07522846533889194 1105 76-77 0.13024362109182164 1105 78-79 0.2221286395372104 1105 80-81 0.33550652559532423 1105 82-83 0.3060094837398637 1105 84-85 0.14254464879248374 1105 86-87 0.1352522564228842 1105 88-89 -0.11521700857321449 1105 90-91 0.08743768463753909 1105 92-93 0.22898333021175787 1105 94-95 0.1943067901683655 1105 96-97 0.6388867640829652 1105 98-99 0.8498694067429042 1105 100-101 0.7392576829759854 1106 1 0.18480955473958005 1106 2 0.04400166470471589 1106 3 0.09740604668998287 1106 4 0.07958497571663514 1106 5 0.08938991680698649 1106 6 0.14130645518227425 1106 7 0.07803118747876425 1106 8 0.11209836424854558 1106 9 0.20040414532771678 1106 10-11 0.1841803648999587 1106 12-13 0.02271546176107364 1106 14-15 0.2056257637572969 1106 16-17 0.2263861405802814 1106 18-19 0.21395407329887206 1106 20-21 0.07454637187543511 1106 22-23 0.1703272913134981 1106 24-25 0.01798833434798297 1106 26-27 0.08542503398566481 1106 28-29 0.06145435143965727 1106 30-31 0.02990128055908059 1106 32-33 0.0926468904867761 1106 34-35 0.12052515779793538 1106 36-37 0.074747865240262 1106 38-39 0.20661380932475737 1106 40-41 0.35506707073788135 1106 42-43 0.2905133675413438 1106 44-45 0.1604718326972545 1106 46-47 0.37740247836283203 1106 48-49 0.40458098515207297 1106 50-51 0.22455570863890273 1106 52-53 0.2571318424876097 1106 54-55 0.25089726878702834 1106 56-57 0.07347913528493422 1106 58-59 0.14281429188908845 1106 60-61 0.16146552926392843 1106 62-63 0.21064338587230935 1106 64-65 0.2590675188700473 1106 66-67 0.08396805495543447 1106 68-69 0.14142915267109402 1106 70-71 0.3462975248218143 1106 72-73 0.48758471662211633 1106 74-75 0.4540450596856971 1106 76-77 0.164991631701902 1106 78-79 0.3142673797184692 1106 80-81 0.5754832198888415 1106 82-83 0.4814694010587388 1106 84-85 0.4041819743914381 1106 86-87 0.5318888839902876 1106 88-89 0.28606081653141757 1106 90-91 0.3213496243959959 1106 92-93 0.6072991937622625 1106 94-95 0.6464675539573399 1106 96-97 0.8602074967596742 1106 98-99 0.8761256498516232 1106 100-101 0.9508423000700681 1107 1 0.16601732051029927 1107 2 0.09204900629656976 1107 3 0.08606694473340326 1107 4 0.19106455467817085 1107 5 0.1292980039083389 1107 6 0.1560590642412194 1107 7 0.1973323354086176 1107 8 0.1129316929086599 1107 9 0.1771615098557291 1107 10-11 0.0932083257122116 1107 12-13 0.23103234535863493 1107 14-15 0.18061889412332022 1107 16-17 0.24623531706538415 1107 18-19 0.3431739913119003 1107 20-21 0.27873937213900746 1107 22-23 0.1545401714566097 1107 24-25 0.21180720593349633 1107 26-27 0.3134169698765561 1107 28-29 0.32812835506233284 1107 30-31 0.28367677531291946 1107 32-33 0.22725891038347612 1107 34-35 0.3442765667738854 1107 36-37 0.31966069098708516 1107 38-39 0.3587519478502159 1107 40-41 0.4154726034569691 1107 42-43 0.2998870531981126 1107 44-45 0.307047670652409 1107 46-47 0.42288800726856834 1107 48-49 0.39948539910219694 1107 50-51 0.3256618599054377 1107 52-53 0.2701889137243114 1107 54-55 0.26280192387532963 1107 56-57 0.18712334993155366 1107 58-59 0.41475940771952224 1107 60-61 0.3827961633451622 1107 62-63 0.4565834321932769 1107 64-65 0.32771394862313485 1107 66-67 0.21941845059028253 1107 68-69 0.3559382402154654 1107 70-71 0.17432142554018526 1107 72-73 0.19975097858790036 1107 74-75 0.2804881034102138 1107 76-77 0.2747996464618012 1107 78-79 0.3141510165307366 1107 80-81 0.4854785373220132 1107 82-83 0.4978299250575944 1107 84-85 0.3647178899594259 1107 86-87 0.26601568341380144 1107 88-89 0.14146469493048386 1107 90-91 0.19755167014495 1107 92-93 0.3638822658625749 1107 94-95 0.3103144929045172 1107 96-97 0.28274620504417314 1107 98-99 0.6057015270618251 1107 100-101 0.7659055848206009 1108 1 0.02504474945393298 1108 2 -0.007682997081680298 1108 3 -0.01423941597735734 1108 4 0.08917698629901594 1108 5 0.0010783184386653488 1108 6 0.06785312360343454 1108 7 0.03249095297660887 1108 8 0.1080177775083726 1108 9 0.04290679438479117 1108 10-11 0.007188724146729442 1108 12-13 -0.014579106459933655 1108 14-15 0.09228074917882623 1108 16-17 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2316 60-61 -0.9127584675756282 2316 62-63 -1.13298068997846 2316 64-65 -0.8924442763779865 2316 66-67 -0.8612341291407262 2316 68-69 -0.9754975926305605 2316 70-71 -0.7732394129286213 2316 72-73 -0.7448691464627046 2316 74-75 -0.6181168851193171 2316 76-77 -0.6019372299720089 2316 78-79 -0.46315657197180826 2316 80-81 -0.5202656937067296 2316 82-83 -0.5906636199921103 2316 84-85 -1.077821806860328 2316 86-87 -0.9137676146834508 2316 88-89 -0.8969270778192815 2316 90-91 -1.1318700832624522 2316 92-93 -1.1445074826867447 2316 94-95 -1.055431537413508 2316 96-97 -1.2335019394387565 2316 98-99 -1.5534174959240978 2316 100-101 -1.6246515201229457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 28.0 6 81.0 7 185.0 8 393.0 9 863.0 10 1461.0 11 2336.0 12 3637.0 13 5145.0 14 6558.0 15 8846.0 16 11634.0 17 15978.0 18 14836.0 19 9691.0 20 8403.0 21 8794.0 22 9415.0 23 10133.0 24 11728.0 25 14245.0 26 17508.0 27 21674.0 28 27205.0 29 34580.0 30 43050.0 31 54157.0 32 69902.0 33 90974.0 34 123250.0 35 177088.0 36 270846.0 37 404040.0 38 294040.0 39 24251.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.5923431371785 19.410232974451517 15.19983790758283 22.79758598078715 2 15.696002885055258 20.59625588392105 39.63711970864504 24.07062152237866 3 19.383499203577944 24.497853389329613 29.918699277487693 26.19994812960475 4 13.292223345101304 17.31422039791471 36.89442438596569 32.49913187101829 5 14.102055388012714 36.95474451092362 34.024955381167544 14.918244719896132 6 31.84028216402175 36.38471225713966 17.341311953628583 14.433693625210006 7 28.36509700501233 32.68058603125596 20.7878933915944 18.166423572137305 8 26.439632514528633 34.82703374404344 19.6487529834961 19.08458075793183 9 26.20159413055443 16.86035414117232 20.274328910881504 36.66372281739175 10-11 24.88251049405972 26.82761917428737 27.18396922818368 21.10590110346923 12-13 25.916830345379477 23.94108289780744 27.90960650051434 22.232480256298743 14-15 22.892019840075147 25.278566296914416 26.18192077882256 25.64749308418787 16-17 22.24345243958292 28.503472796105882 26.837944158128668 22.41513060618253 18-19 22.11258489696608 26.871934971873536 29.454099429901987 21.5613807012584 20-21 22.748542453941877 26.026779391538348 29.643508149820125 21.58117000469965 22-23 22.488200965069513 25.94969640544717 29.574383308782732 21.98771932070058 24-25 22.39889363170402 26.10795438735134 29.681696505590278 21.811455475354364 26-27 22.24668782216029 26.328321610993438 29.434528347879123 21.99046221896715 28-29 22.112673077661565 26.334713372428563 29.661666877225123 21.890946672684745 30-31 22.246991171133057 26.170882552689296 29.610271768779434 21.971854507398213 32-33 22.169002562668513 26.217879690921585 29.48835669229335 22.124761054116547 34-35 22.453664605398885 26.280844233992244 29.37577895803901 21.889712202569854 36-37 22.490469927605403 26.134105052594197 29.24690025663988 22.128524763160517 38-39 22.32373447089384 26.350331347889544 29.298165794991448 22.027768386225166 40-41 22.51792795425137 26.15499993043091 29.363776766707016 21.963295348610707 42-43 22.369045310140432 26.2957590456524 29.05998008204816 22.27521556215901 44-45 22.534996109997472 26.42736380260825 28.841113152883217 22.196526934511056 46-47 22.7023781268095 26.63453610986903 28.413338345559215 22.249747417762254 48-49 22.762964657198363 26.761171612009953 28.172819059925665 22.303044670866022 50-51 22.691099639604744 26.86769999265408 28.036118549781957 22.405081817959214 52-53 22.86512078289409 26.94565291491425 27.86725637861399 22.321969923577672 54-55 22.80646153118422 26.898328688870123 27.838286422631775 22.456923357313883 56-57 22.68566065529205 27.1951793993671 27.762667335205204 22.356492610135646 58-59 22.79175437302878 26.905691719731045 27.907220697263842 22.395333209976336 60-61 22.752457819933962 26.85795549560273 28.10260725215829 22.286979432305017 62-63 22.68314406529003 26.912160020790594 28.11474226147232 22.289953652447053 64-65 22.695936174670944 26.864695633977732 27.995374105061575 22.44399408628975 66-67 22.613395868682446 27.195141564322352 27.750357966272983 22.44110460072222 68-69 22.6496841870948 26.91699824702969 28.01096302067392 22.422354545201593 70-71 22.67256496316162 26.832945350973315 27.961303420384965 22.533186265480108 72-73 22.69940220939312 26.83765930554775 27.818968679022454 22.64396980603668 74-75 22.64248019823332 27.09496639299672 27.580810547214675 22.681742861555286 76-77 22.70256237167203 27.15523514122442 27.655343800007735 22.48685868709582 78-79 22.661553492563012 27.150431337104674 27.585177286353797 22.602837883978513 80-81 22.701013819960977 27.286938094543807 27.45836593458084 22.553682150914376 82-83 22.64553527105812 27.296113716856514 27.57851925889484 22.479831753190524 84-85 22.667484717202154 27.16987904538511 27.338803698478813 22.82383253893392 86-87 22.67162093503004 27.033131901552004 27.518532595720906 22.776714567697052 88-89 22.736740822195905 27.08381563365358 27.41638025447649 22.763063289674022 90-91 22.66518897720832 27.093054089722614 27.551859415080948 22.689897517988122 92-93 22.64327499048395 26.90791245678823 27.527262649669108 22.921549903058715 94-95 22.569315078403356 26.906145526535823 27.524774514700546 22.999764880360278 96-97 22.640554468691214 26.902104578084085 27.476768448552235 22.980572504672466 98-99 22.461081623135886 26.85835458211229 27.427313594135892 23.253250200615927 100-101 22.317293597944076 27.14777339876257 27.325129079542382 23.209803923750968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 551.0 1 412.0 2 308.0 3 344.0 4 299.0 5 287.0 6 501.5 7 1083.5 8 1855.5 9 2241.5 10 1965.0 11 1643.5 12 1681.0 13 1869.5 14 2080.5 15 2241.0 16 2385.5 17 2526.0 18 2733.0 19 3042.0 20 3409.0 21 3891.0 22 4557.0 23 5528.5 24 6804.0 25 8435.5 26 10713.5 27 13379.5 28 15775.5 29 18235.0 30 20853.5 31 23731.0 32 26984.0 33 30146.0 34 33656.5 35 37248.0 36 41495.0 37 45307.5 38 47474.5 39 48879.0 40 50691.5 41 53446.0 42 55625.5 43 57868.0 44 60599.0 45 62919.5 46 64000.5 47 65185.5 48 67605.5 49 68044.0 50 66805.5 51 65727.5 52 64192.5 53 63357.0 54 62562.5 55 58921.0 56 54107.5 57 49116.5 58 44339.0 59 39608.5 60 33772.5 61 27910.5 62 22158.5 63 16648.0 64 12145.0 65 9024.5 66 6722.0 67 5274.0 68 3940.0 69 2753.5 70 1949.0 71 1230.5 72 827.5 73 574.0 74 394.5 75 273.0 76 164.5 77 78.5 78 37.5 79 27.5 80 16.5 81 13.0 82 14.5 83 9.0 84 4.0 85 1.5 86 0.5 87 1.0 88 0.5 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025765608644333873 2 0.006844859315881353 3 0.010183815079725916 4 5.5649262730742715E-5 5 0.004563239543920902 6 0.0 7 0.0 8 0.0 9 1.1129852546148543E-4 10-11 0.0 12-13 1.947724195575995E-4 14-15 0.0 16-17 1.1129852546148543E-4 18-19 0.004730187332113131 20-21 0.0015303547250954245 22-23 0.0 24-25 0.005509277010343529 26-27 0.0063996652140354115 28-29 0.008903882036918834 30-31 0.03277741574840746 32-33 7.79089678230398E-4 34-35 0.005425803116247414 36-37 0.0160269876664539 38-39 0.007874370676400094 40-41 0.010795956969764086 42-43 0.005425803116247414 44-45 0.003060709450190849 46-47 0.00164165325055691 48-49 0.0014468808309993104 50-51 0.002949410924729364 52-53 0.0025876907169795358 54-55 0.001474705462364682 56-57 8.069143095957692E-4 58-59 9.182128350572547E-4 60-61 2.7824631365371358E-5 62-63 1.391231568268568E-4 64-65 0.007095280998169696 66-67 6.677911527689126E-4 68-69 0.0010573359918841115 70-71 0.003672851340229019 72-73 0.010350762867918143 74-75 0.005370153853516671 76-77 0.003311131132479192 78-79 0.009599497821053118 80-81 1.6694778819222815E-4 82-83 0.004730187332113131 84-85 7.79089678230398E-4 86-87 0.0 88-89 0.0013912315682685678 90-91 0.001085160623249483 92-93 2.782463136537136E-4 94-95 6.399665214035412E-4 96-97 3.0607094501908493E-4 98-99 1.6694778819222815E-4 100-101 8.347389409611407E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1796969.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.63173038265916 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2813321926951 48.862456900782206 2 12.40602216251222 15.292092260824944 3 3.6740169611815867 6.793080683185808 4 1.4901939925700862 3.673729374717518 5 0.824788069378627 2.5416557957388757 6 0.5094971414908495 1.8840714273059747 7 0.3459353747434201 1.4924417022207441 8 0.24777846286202576 1.2216812334193676 9 0.17939255548690408 0.9950646251182585 >10 0.9535151824688569 10.638485321358832 >50 0.0609986825365064 2.583104672921656 >100 0.025533479794353223 2.6686077611353447 >500 5.431321525046585E-4 0.1960470813166933 >1k 2.715660762523291E-4 0.3849530054311847 >5k 1.8104405083488607E-4 0.7725281545225529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7724 0.42983490533225666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6123 0.34074043570033763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.231222686646236 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.564926273074271E-5 0.0 3 0.0 0.0 0.0 5.564926273074271E-5 0.0 4 0.0 0.0 0.0 5.564926273074271E-5 0.0 5 0.0 0.0 0.0 5.564926273074271E-5 0.0 6 0.0 0.0 0.0 5.564926273074271E-5 0.0 7 0.0 0.0 0.0 5.564926273074271E-5 0.0 8 0.0 0.0 0.0 5.564926273074271E-5 0.0 9 0.0 0.0 0.0 5.564926273074271E-5 0.0 10-11 0.0 0.0 0.0 5.564926273074271E-5 0.0 12-13 0.0 0.0 0.0 5.564926273074271E-5 0.0 14-15 0.0 0.0 0.0 1.1129852546148542E-4 0.0 16-17 0.0 0.0 0.0 1.391231568268568E-4 0.0 18-19 0.0 0.0 0.0 2.504216822883422E-4 0.0 20-21 0.0 0.0 0.0 3.338955763844563E-4 0.0 22-23 0.0 0.0 0.0 5.008433645766844E-4 0.0 24-25 0.0 0.0 0.0 6.677911527689126E-4 0.0 26-27 0.0 0.0 0.0 8.62563572326512E-4 0.0 28-29 5.564926273074271E-5 0.0 0.0 0.0014468808309993104 0.0 30-31 5.564926273074271E-5 0.0 0.0 0.0028937616619986208 2.7824631365371354E-5 32-33 5.564926273074271E-5 0.0 0.0 0.004674538069382388 1.6694778819222815E-4 34-35 1.1129852546148543E-4 0.0 0.0 0.0058431725867279845 1.6694778819222815E-4 36-37 1.6694778819222815E-4 0.0 0.0 0.007846546045034722 1.6694778819222815E-4 38-39 2.2259705092297083E-4 0.0 0.0 0.011046378652052428 2.2259705092297083E-4 40-41 2.2259705092297083E-4 0.0 0.0 0.014580106835454592 2.782463136537136E-4 42-43 2.504216822883422E-4 0.0 0.0 0.01819730891295287 2.782463136537136E-4 44-45 2.782463136537136E-4 0.0 0.0 0.01972766363804829 2.782463136537136E-4 46-47 2.782463136537136E-4 0.0 0.0 0.02259360066868154 2.782463136537136E-4 48-49 2.782463136537136E-4 0.0 0.0 0.026099504220718335 4.173694704805703E-4 50-51 2.782463136537136E-4 0.0 0.0 0.030301023556889406 4.4519410184594167E-4 52-53 2.782463136537136E-4 0.0 0.0 0.038704062229231555 5.008433645766844E-4 54-55 2.782463136537136E-4 0.0 0.0 0.043016880090864115 5.008433645766844E-4 56-57 2.782463136537136E-4 0.0 0.0 0.05704049429901128 5.008433645766844E-4 58-59 2.782463136537136E-4 0.0 0.0 0.08236090884149921 5.008433645766844E-4 60-61 2.782463136537136E-4 0.0 0.0 0.10019649754670225 5.008433645766844E-4 62-63 2.782463136537136E-4 0.0 0.0 0.1113263500928508 5.008433645766844E-4 64-65 2.782463136537136E-4 0.0 0.0 0.13698066021172317 5.008433645766844E-4 66-67 2.782463136537136E-4 0.0 0.0 0.18898489623360226 5.008433645766844E-4 68-69 2.782463136537136E-4 0.0 0.0 0.2646957181787777 5.008433645766844E-4 70-71 2.782463136537136E-4 0.0 0.0 0.3256038362375756 5.008433645766844E-4 72-73 3.0607094501908493E-4 0.0 0.0 0.3983930718893871 6.121418900381699E-4 74-75 3.338955763844563E-4 0.0 0.0 0.4811157009386361 6.677911527689126E-4 76-77 3.338955763844563E-4 0.0 0.0 0.5350120118933605 7.234404154996553E-4 78-79 3.338955763844563E-4 0.0 0.0 0.6003442463392523 7.512650468650267E-4 80-81 3.338955763844563E-4 0.0 0.0 0.6933341643623234 8.069143095957693E-4 82-83 3.338955763844563E-4 0.0 0.0 0.7757785470979188 8.347389409611407E-4 84-85 3.338955763844563E-4 0.0 0.0 0.8921689800992672 8.347389409611407E-4 86-87 3.338955763844563E-4 0.0 0.0 1.0804860851801004 8.903882036918834E-4 88-89 3.338955763844563E-4 0.0 0.0 1.3084254653252225 9.46037466422626E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3695 0.0 51.176357 1 GTATCAA 6985 0.0 43.19247 1 TATCAAC 9045 0.0 33.502705 2 ATCAACG 8995 0.0 33.423058 3 TCAACGC 9140 0.0 32.83903 4 CAACGCA 9305 0.0 32.307755 5 AACGCAG 9560 0.0 31.594143 6 ACGCAGA 10955 0.0 27.397572 7 CGCAGAG 11075 0.0 27.05783 8 GTGGTAT 1880 0.0 24.514025 1 TGGTATC 1805 0.0 24.472229 2 GCAGAGT 12060 0.0 24.257204 9 GTACATG 6565 0.0 22.1456 1 GAGTACT 7300 0.0 21.79483 12-13 TACATGG 6665 0.0 21.165283 2 AGAGTAC 10725 0.0 20.811718 10-11 AGTACTT 7670 0.0 20.433846 12-13 ACATGGG 6495 0.0 20.328684 3 CAGAGTA 11480 0.0 20.24968 10-11 GTACTTT 7815 0.0 19.995054 14-15 >>END_MODULE