FastQCFastQC Report
Thu 26 May 2016
SRR936865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826453
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66180.36234165346713004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47480.2599574147267956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30540.1672093396326103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.050.4839441
GTATCAA62500.040.6722371
ATCAACG79800.031.5614413
TCAACGC80150.031.4236154
TATCAAC80650.031.405572
CAACGCA81800.030.7897645
AACGCAG84400.029.9546956
ACGCAGA95500.026.172337
CGCAGAG95500.026.0230588
GTACATG68950.023.7743781
TACATGG68850.023.052942
GCAGAGT105650.022.5753639
ACATGGG70650.021.5239333
GAGTACT59400.021.51476712-13
GTGGTAT19250.019.9930291
CAGAGTA100950.019.88478710-11
AGAGTAC95100.019.8589910-11
TGGTATC18950.019.8107852
AGTACTT63150.019.71054812-13
GTACTTT65400.019.32009514-15
Produced by FastQC (version 0.11.5)