FastQCFastQC Report
Thu 26 May 2016
SRR936865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826453
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.42026813720364004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61130.33469243391425896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.22469781593065902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.048.1317941
GTATCAA72900.040.6070861
ATCAACG93400.031.326273
TATCAAC93750.031.2616942
TCAACGC94400.030.9441094
CAACGCA96700.030.2089335
AACGCAG99150.029.5103746
ACGCAGA112650.025.8051837
CGCAGAG112250.025.7701918
GCAGAGT124750.022.6549519
TGGTATC22500.022.589152
GTGGTAT23700.022.2544021
GAGTACT72800.021.92152812-13
GTACATG71150.020.5691871
GTACTTT77450.020.29903614-15
AGTACTT75750.020.28404612-13
AGAGTAC111950.020.08957510-11
ACATGGG69350.020.0676253
TACATGG72550.019.9037442
CAGAGTA119700.019.64200810-11
Produced by FastQC (version 0.11.5)