##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1826453 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.190522285544716 33.0 31.0 34.0 31.0 34.0 2 32.329364620934676 34.0 31.0 34.0 31.0 34.0 3 32.436189160082414 34.0 31.0 34.0 31.0 34.0 4 36.081198913960556 37.0 35.0 37.0 35.0 37.0 5 35.98651594100697 37.0 35.0 37.0 35.0 37.0 6 36.02195621787147 37.0 35.0 37.0 35.0 37.0 7 35.97298698625149 37.0 35.0 37.0 35.0 37.0 8 35.932340990980876 37.0 35.0 37.0 35.0 37.0 9 37.62337985154833 39.0 37.0 39.0 35.0 39.0 10-11 37.539116801801086 39.0 37.0 39.0 35.0 39.0 12-13 37.4388547091001 39.0 37.0 39.0 34.0 39.0 14-15 38.65761369167451 40.0 38.0 41.0 34.0 41.0 16-17 38.56704114477624 40.0 38.0 41.0 34.0 41.0 18-19 38.71714848397413 40.0 38.0 41.0 34.0 41.0 20-21 38.741449136659966 40.0 38.0 41.0 34.0 41.0 22-23 38.66788496610644 40.0 38.0 41.0 34.0 41.0 24-25 38.5657161722749 40.0 38.0 41.0 34.0 41.0 26-27 38.38757717827943 40.0 38.0 41.0 34.0 41.0 28-29 38.30650802402252 40.0 38.0 41.0 34.0 41.0 30-31 38.29849905801025 40.0 38.0 41.0 34.0 41.0 32-33 38.13963376008033 40.0 37.5 41.0 33.5 41.0 34-35 37.99681103209335 40.0 37.0 41.0 33.0 41.0 36-37 37.84203097479103 40.0 37.0 41.0 33.0 41.0 38-39 37.694233303567074 40.0 37.0 41.0 33.0 41.0 40-41 37.511976765895426 40.0 37.0 41.0 33.0 41.0 42-43 37.2651491716458 40.0 36.5 41.0 32.0 41.0 44-45 37.013717845463304 40.0 36.0 41.0 31.0 41.0 46-47 36.679975613935866 39.0 36.0 41.0 30.5 41.0 48-49 36.330545872245274 39.0 35.5 41.0 30.0 41.0 50-51 36.04070047244578 39.0 35.0 40.0 29.5 41.0 52-53 35.79067706642328 39.0 35.0 40.0 28.5 41.0 54-55 35.498649020806994 38.0 35.0 40.0 28.0 41.0 56-57 35.204585609375115 38.0 34.5 40.0 27.5 41.0 58-59 34.83548139481279 38.0 34.0 40.0 26.5 41.0 60-61 34.49409292218305 37.5 34.0 40.0 26.0 41.0 62-63 34.10821740280204 37.0 33.0 39.5 26.0 41.0 64-65 33.711450280954395 36.0 33.0 39.0 25.5 40.5 66-67 33.3050196199957 36.0 32.0 39.0 24.0 40.0 68-69 32.991141847066416 35.5 32.0 38.5 23.5 40.0 70-71 32.51654271968674 35.0 32.0 38.0 22.5 40.0 72-73 32.01117192722725 35.0 31.0 37.0 21.5 39.0 74-75 31.491271606770063 34.5 31.0 36.5 20.0 39.0 76-77 30.100286183110107 33.5 29.5 35.0 19.5 37.5 78-79 30.84907878823052 34.0 30.5 36.0 19.5 38.0 80-81 30.64441652755368 34.0 30.5 35.5 18.0 37.0 82-83 30.305999661639255 34.0 30.0 35.0 17.0 37.0 84-85 29.93616123710821 34.0 30.0 35.0 12.5 36.0 86-87 29.571554811429586 34.0 29.5 35.0 7.5 36.0 88-89 29.163948648007917 34.0 29.0 35.0 4.5 35.5 90-91 28.68478849442061 34.0 29.0 35.0 2.0 35.0 92-93 28.17641871978091 33.0 28.5 35.0 2.0 35.0 94-95 27.570735737519662 33.0 27.0 35.0 2.0 35.0 96-97 26.878257748762216 33.0 25.5 34.0 2.0 35.0 98-99 25.99047333821347 32.0 24.5 34.0 2.0 35.0 100-101 22.356504930594983 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22641820738896712 1101 2 0.23998677758211073 1101 3 0.295546554273713 1101 4 0.25465914933506184 1101 5 0.2745859305396081 1101 6 0.23243490224270857 1101 7 0.20751369118986673 1101 8 0.3103074193503872 1101 9 0.2893496999262979 1101 10-11 0.3262944700151209 1101 12-13 0.38734012313044275 1101 14-15 0.4351766586910415 1101 16-17 0.429474820267167 1101 18-19 0.4144041367479403 1101 20-21 0.33611551411225093 1101 22-23 0.3860666219997384 1101 24-25 0.3790556863450689 1101 26-27 0.2010767749906961 1101 28-29 0.3042355502143721 1101 30-31 0.34203465277407474 1101 32-33 0.43862469863402254 1101 34-35 0.41621243907572847 1101 36-37 0.285229927872102 1101 38-39 0.376267780519143 1101 40-41 0.4018587821505335 1101 42-43 0.48407219518872324 1101 44-45 0.5008010221113821 1101 46-47 0.4243881946223027 1101 48-49 0.4224972725090623 1101 50-51 0.4133100026826284 1101 52-53 0.44215457297650573 1101 54-55 0.41668795325907126 1101 56-57 0.3887258011327859 1101 58-59 0.3991684957376407 1101 60-61 0.573021255083404 1101 62-63 0.45130914666284383 1101 64-65 0.556403222890701 1101 66-67 0.3793966962720958 1101 68-69 0.3976869518003028 1101 70-71 0.3293237781945635 1101 72-73 0.5150423722255333 1101 74-75 0.44523534902338113 1101 76-77 0.21374212478111687 1101 78-79 0.3572503716105935 1101 80-81 0.5280579155142888 1101 82-83 0.4345876734778038 1101 84-85 0.3789882097418875 1101 86-87 0.07973968484469651 1101 88-89 0.22002597063732665 1101 90-91 0.5358534251683196 1101 92-93 0.5585721417152385 1101 94-95 0.3342916487085539 1101 96-97 0.33310765738296766 1101 98-99 0.5603570142512062 1101 100-101 1.0155531566190419 1102 1 0.29793847476169333 1102 2 0.30062358615220575 1102 3 0.24638320071588993 1102 4 0.1832415952532287 1102 5 0.13654895098819964 1102 6 0.10731870134796395 1102 7 0.1645791897608717 1102 8 0.2687569535784675 1102 9 0.3000042152993956 1102 10-11 0.2620379689900645 1102 12-13 0.24593055094339888 1102 14-15 0.38982234279614403 1102 16-17 0.3878648707038863 1102 18-19 0.3380106943517731 1102 20-21 0.32110432977612646 1102 22-23 0.2961164085497643 1102 24-25 0.3379904284759192 1102 26-27 0.3604488959060248 1102 28-29 0.45672570772191534 1102 30-31 0.4751329213807409 1102 32-33 0.43852769163706995 1102 34-35 0.39254549850694787 1102 36-37 0.34284222773680284 1102 38-39 0.3858289353900375 1102 40-41 0.4112982027507357 1102 42-43 0.477217206988648 1102 44-45 0.3055491082549793 1102 46-47 0.4806359985637272 1102 48-49 0.36395899676434595 1102 50-51 0.42530515129526947 1102 52-53 0.3380041606016704 1102 54-55 0.31715877438439577 1102 56-57 0.37801059154234196 1102 58-59 0.3601820580420707 1102 60-61 0.4572293114021022 1102 62-63 0.30465721952633373 1102 64-65 0.3260480908228587 1102 66-67 0.43589557714681604 1102 68-69 0.4148426651492869 1102 70-71 0.3473333606183502 1102 72-73 0.3375716161173763 1102 74-75 0.32501480249183956 1102 76-77 0.26811186634479256 1102 78-79 0.4315976753348316 1102 80-81 0.5483975679629438 1102 82-83 0.3636236833050752 1102 84-85 0.3586850429908388 1102 86-87 0.3664129984792126 1102 88-89 0.47661881515223214 1102 90-91 0.7351991726305762 1102 92-93 0.576496994319939 1102 94-95 0.26419536105047214 1102 96-97 0.16735641859842687 1102 98-99 0.1980839518319577 1102 100-101 0.5798862603172736 1103 1 0.2288791169870379 1103 2 0.2661300317179496 1103 3 0.21098797993286666 1103 4 0.13399963077657162 1103 5 0.16425688815670014 1103 6 0.14668953860682166 1103 7 0.03829291840168736 1103 8 0.10122330003797941 1103 9 0.26718587216911516 1103 10-11 0.22636531240741675 1103 12-13 0.28897933918474905 1103 14-15 0.4096551491941227 1103 16-17 0.4402862687105866 1103 18-19 0.3704095121670008 1103 20-21 0.2879782063056382 1103 22-23 0.2720729359012708 1103 24-25 0.20854254498354408 1103 26-27 0.36803117777694183 1103 28-29 0.2500668632034859 1103 30-31 0.2844362685280686 1103 32-33 0.32852343376820414 1103 34-35 0.4477219881348944 1103 36-37 0.26173560569106513 1103 38-39 0.23741571403287764 1103 40-41 0.2636753844268753 1103 42-43 0.3394289097701346 1103 44-45 0.2863203027124328 1103 46-47 0.29310818273486205 1103 48-49 0.34161963498227266 1103 50-51 0.4246125251149664 1103 52-53 0.35082882310294394 1103 54-55 0.2770476266671693 1103 56-57 0.29931611964330784 1103 58-59 0.43334033709239606 1103 60-61 0.2870320815215024 1103 62-63 0.4295177791374627 1103 64-65 0.4105773843826839 1103 66-67 0.27717966177244335 1103 68-69 0.06337244807505016 1103 70-71 0.03984201603729787 1103 72-73 0.33102779185754017 1103 74-75 0.3158753158636678 1103 76-77 0.1913570367462505 1103 78-79 0.17592806283942863 1103 80-81 0.23184091229737902 1103 82-83 0.23028688202424874 1103 84-85 0.2685082932668301 1103 86-87 0.029228798447999083 1103 88-89 0.30381388141892884 1103 90-91 0.08804934381231888 1103 92-93 0.10695905979093467 1103 94-95 -0.058881999224961845 1103 96-97 0.07725191778057194 1103 98-99 0.05398543515017096 1103 100-101 0.16200725700538143 1104 1 0.22494523883171524 1104 2 0.2440352246958284 1104 3 0.23122418313629822 1104 4 0.10712513680659441 1104 5 0.09966996447234777 1104 6 0.09705699651776456 1104 7 0.17208740421050095 1104 8 0.15145763934577872 1104 9 0.22814108046385684 1104 10-11 0.3212690571913086 1104 12-13 0.20253133040738902 1104 14-15 0.038840462166895406 1104 16-17 0.3461643899273028 1104 18-19 0.26308954714003363 1104 20-21 0.27240218317686526 1104 22-23 0.2002835549321773 1104 24-25 0.2403363710791382 1104 26-27 0.11862489027486589 1104 28-29 0.03786381499914171 1104 30-31 0.16772171091517407 1104 32-33 0.1237148182193124 1104 34-35 0.09499193960893138 1104 36-37 0.10792893694294747 1104 38-39 0.18766748693865054 1104 40-41 0.06748272935163868 1104 42-43 0.16061105470830483 1104 44-45 0.14408536836918273 1104 46-47 0.20780893928833422 1104 48-49 0.18129962115299492 1104 50-51 0.20370502549274505 1104 52-53 0.21717398247189834 1104 54-55 0.25100527096179803 1104 56-57 0.18032402267839842 1104 58-59 0.3836595806682723 1104 60-61 0.5205179367879538 1104 62-63 0.47452027511126715 1104 64-65 0.282496297278783 1104 66-67 0.382223263406793 1104 68-69 0.31081267846901284 1104 70-71 0.33974870365956633 1104 72-73 0.5289097125990558 1104 74-75 0.5450511329643497 1104 76-77 0.41109695568736626 1104 78-79 0.3153010060129553 1104 80-81 0.7457073844603634 1104 82-83 0.5528619508619705 1104 84-85 0.37749988456919326 1104 86-87 0.30309754021260815 1104 88-89 0.24770033313197715 1104 90-91 0.5383912246064888 1104 92-93 0.2746291138086292 1104 94-95 0.30912405578995106 1104 96-97 0.11632449152645208 1104 98-99 0.12109464473494214 1104 100-101 0.9191357758433725 1105 1 0.17144316118986325 1105 2 0.17933018462449013 1105 3 0.13239080987103335 1105 4 0.07019996168499176 1105 5 0.15475363013859322 1105 6 0.17682029704075575 1105 7 0.14129152064917605 1105 8 0.09669125532157352 1105 9 0.1528718943522307 1105 10-11 0.19924803834579308 1105 12-13 0.11233289469944907 1105 14-15 0.1122204189837035 1105 16-17 0.08186240245128573 1105 18-19 0.09663633909372749 1105 20-21 0.16266852679656552 1105 22-23 0.09615279746833494 1105 24-25 0.0857416707316574 1105 26-27 0.1261881871118291 1105 28-29 0.20665424607970806 1105 30-31 0.2109465853422421 1105 32-33 0.11176557761123007 1105 34-35 0.1904157402687261 1105 36-37 0.12921728754625406 1105 38-39 0.3610002342886105 1105 40-41 0.3066850169551145 1105 42-43 0.26168935862669684 1105 44-45 0.28877118440536975 1105 46-47 0.26633917957459374 1105 48-49 0.29304613041696115 1105 50-51 0.40094702859183684 1105 52-53 0.4905959032592335 1105 54-55 0.4666568395778512 1105 56-57 0.5445992155921573 1105 58-59 0.4913721614949935 1105 60-61 0.48272385229009274 1105 62-63 0.4963000011262366 1105 64-65 0.46774808819046854 1105 66-67 0.3848114196431851 1105 68-69 0.5399338310037862 1105 70-71 0.34641728894921187 1105 72-73 0.3866294528193954 1105 74-75 0.4949383547935575 1105 76-77 0.4378305567772962 1105 78-79 0.6359838562171767 1105 80-81 0.5456566307392521 1105 82-83 0.546087321484638 1105 84-85 0.6215022266124173 1105 86-87 0.47495587778723447 1105 88-89 0.3680276237480662 1105 90-91 0.40770308637490515 1105 92-93 0.3104435196427531 1105 94-95 0.04041868681181171 1105 96-97 0.21806089257725247 1105 98-99 0.046053992138492816 1105 100-101 0.42758315146485515 1106 1 0.19128229958298704 1106 2 0.17768101520943702 1106 3 0.12849603339032 1106 4 0.12601653917568 1106 5 0.1416171046775716 1106 6 0.08711712270688565 1106 7 0.18164789352478294 1106 8 0.15843331121070747 1106 9 0.10170598564691602 1106 10-11 0.13270579044898767 1106 12-13 0.1546801058292786 1106 14-15 0.24193471100365826 1106 16-17 0.23347288066351268 1106 18-19 -0.01804645511459313 1106 20-21 0.2756302866462761 1106 22-23 0.14925105932984195 1106 24-25 0.11975199666193248 1106 26-27 0.1610568922048614 1106 28-29 0.15697970737402045 1106 30-31 0.11196441915904387 1106 32-33 0.1465487620420589 1106 34-35 0.019493868911119705 1106 36-37 0.09094223930294021 1106 38-39 0.14759156001061058 1106 40-41 0.3308215686240743 1106 42-43 0.3139315862468308 1106 44-45 0.03596203755673599 1106 46-47 0.005154387355325696 1106 48-49 -0.021753909860507292 1106 50-51 0.043600320930430314 1106 52-53 0.08976207680272807 1106 54-55 -0.11931763388384553 1106 56-57 0.017826972288631282 1106 58-59 0.25903983371912176 1106 60-61 0.0831590421114754 1106 62-63 0.2999733894410852 1106 64-65 -0.08458710866568708 1106 66-67 -0.08703269550073145 1106 68-69 -0.1613121498699286 1106 70-71 -0.308172894658135 1106 72-73 -0.2665850895554023 1106 74-75 -0.10984280196995755 1106 76-77 -0.0109211863958798 1106 78-79 0.0937845130710393 1106 80-81 0.03591706874297529 1106 82-83 0.14687245633831125 1106 84-85 0.08625516155739987 1106 86-87 -0.1273476626302461 1106 88-89 -0.12703107515160994 1106 90-91 -0.1652359691183527 1106 92-93 0.039970769698271624 1106 94-95 0.07037878176733514 1106 96-97 0.19199502235624166 1106 98-99 0.09853002221418095 1106 100-101 0.28404223741449997 1107 1 0.06965973882802245 1107 2 0.09878403444541561 1107 3 0.13471791052960924 1107 4 0.16075968005338837 1107 5 0.09924140043280971 1107 6 0.07778310879333361 1107 7 0.09476607723830455 1107 8 0.10730432288681158 1107 9 0.1443740518165697 1107 10-11 0.16185171819085298 1107 12-13 0.14884205356678137 1107 14-15 0.3019280170339016 1107 16-17 0.18669696968851923 1107 18-19 0.20721681040834738 1107 20-21 0.16664798572597306 1107 22-23 0.2315361633300057 1107 24-25 0.19638556891726466 1107 26-27 0.26664108485060467 1107 28-29 0.22486319076250538 1107 30-31 0.23367404944901438 1107 32-33 0.3392076819165126 1107 34-35 0.12899299541995646 1107 36-37 0.29516982468900466 1107 38-39 0.35543293050106683 1107 40-41 0.36704009877848875 1107 42-43 0.25817792752905433 1107 44-45 0.17031192768137515 1107 46-47 0.27960395212746647 1107 48-49 0.25981099328861745 1107 50-51 0.10476187645563328 1107 52-53 0.1579546969806529 1107 54-55 0.2924529690447315 1107 56-57 0.1970388412242201 1107 58-59 0.2275310464859146 1107 60-61 0.361281902816998 1107 62-63 0.32264741468166136 1107 64-65 0.20241324999689425 1107 66-67 0.16695193393052676 1107 68-69 0.17045193482555732 1107 70-71 0.3390085686372686 1107 72-73 0.3035326460295984 1107 74-75 0.306300049581278 1107 76-77 0.42072363700074433 1107 78-79 0.5952347400509304 1107 80-81 0.6195701230064685 1107 82-83 0.5700459731407328 1107 84-85 0.636202320734089 1107 86-87 0.6863227336479731 1107 88-89 0.5204671135624181 1107 90-91 0.6002570161924616 1107 92-93 0.5646529858655711 1107 94-95 0.5263068191960514 1107 96-97 0.49323164939428565 1107 98-99 0.47465204098125824 1107 100-101 0.7128125679512252 1108 1 0.09350956151297396 1108 2 0.12389497487893664 1108 3 0.09310813639295645 1108 4 0.12174562759847873 1108 5 0.14849373776758767 1108 6 0.13842343421860193 1108 7 0.16555934874842393 1108 8 0.16615016309465602 1108 9 0.16849174349698615 1108 10-11 0.15479704446951104 1108 12-13 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-1.088630314492491 2316 52-53 -0.9514507521871565 2316 54-55 -1.0779776034381285 2316 56-57 -1.0088401783928518 2316 58-59 -0.8529086623818998 2316 60-61 -0.8883980040523198 2316 62-63 -1.1157372003900008 2316 64-65 -1.155895920155487 2316 66-67 -1.352727909007342 2316 68-69 -1.6037275195834688 2316 70-71 -1.3614496454784515 2316 72-73 -0.9617010553745118 2316 74-75 -1.1321856934154688 2316 76-77 -0.9918874511531648 2316 78-79 -1.199641503197764 2316 80-81 -1.1676448081248338 2316 82-83 -0.9357082929756579 2316 84-85 -1.0906751279129736 2316 86-87 -1.109618258788938 2316 88-89 -1.2405251722367225 2316 90-91 -1.07445382477923 2316 92-93 -1.044249874950438 2316 94-95 -1.034801327820702 2316 96-97 -0.6774475873572321 2316 98-99 -0.893773699446605 2316 100-101 -0.8947988776375908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 7.0 5 38.0 6 79.0 7 173.0 8 466.0 9 942.0 10 1545.0 11 2535.0 12 3658.0 13 5374.0 14 6821.0 15 9112.0 16 12315.0 17 16536.0 18 15320.0 19 10164.0 20 9329.0 21 9562.0 22 10315.0 23 11136.0 24 13042.0 25 15606.0 26 19311.0 27 23907.0 28 30044.0 29 37327.0 30 46780.0 31 58500.0 32 74449.0 33 97121.0 34 131439.0 35 186872.0 36 282333.0 37 404133.0 38 263319.0 39 16833.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.596759910117385 19.384568032741544 15.163447588826998 22.855224468314077 2 15.680130270330647 20.601952740867606 39.56824408822814 24.149672900573602 3 19.408459356424014 24.479386526109927 29.934720394907938 26.17743372255812 4 13.374341959380107 17.284341986755692 36.818738040291386 32.52257801357282 5 14.076252756710824 36.99259548019877 34.120086375114155 14.811065387976253 6 31.89668718549013 36.386975191806194 17.25765732816558 14.458680294538102 7 28.3302116178188 32.64524189782053 20.81646776566383 18.20807871869684 8 26.433037149053384 34.79509190764832 19.628755845346145 19.14311509795215 9 26.187384064842657 16.844899291084573 20.284135580896734 36.683581063176035 10-11 24.8984944599741 26.783691215082232 27.20551073097678 21.11230359396689 12-13 25.922156223018057 23.944361010110853 27.909040090273336 22.224442676597754 14-15 22.849123410238313 25.284800649127025 26.234291273851557 25.6317846667831 16-17 22.23290169525304 28.480886176649495 26.814870133532043 22.47134199456542 18-19 22.128013709692873 26.87734916737159 29.359082808406495 21.635554314529042 20-21 22.74726694651937 26.05794779672116 29.57857292641356 21.616212330345913 22-23 22.512657295677823 25.930469328745847 29.54139169751486 22.015481678061473 24-25 22.37345834749033 26.1853479253759 29.60722991118669 21.83396381594708 26-27 22.30278045919929 26.33529772174382 29.337729343670144 22.02419247538674 28-29 22.153974945337847 26.336577611224854 29.609846223253733 21.899601220183566 30-31 22.269893219339057 26.20455730717269 29.494485759612793 22.03106371387546 32-33 22.207359617512115 26.21459587479676 29.468584617970677 22.109459889720444 34-35 22.49031319174378 26.26659432243808 29.289447908049098 21.95364457776904 36-37 22.529175816549156 26.088177808830267 29.194707217905506 22.18793915671507 38-39 22.319661877399618 26.359084690271033 29.259312293851476 22.061941138477874 40-41 22.5393384725183 26.19525198002983 29.29595502031428 21.969454527137586 42-43 22.430716804648135 26.24179215123521 29.047667802018445 22.27982324209821 44-45 22.543996478420194 26.40429291090436 28.80648995621568 22.245220654459764 46-47 22.70064997833223 26.63213876351635 28.338650126707858 22.32856113144356 48-49 22.727683369588487 26.76971781207584 28.14145477979443 22.361144038541244 50-51 22.755879384933465 26.91781208198478 27.952012194888244 22.37429633819351 52-53 22.880139811974356 26.9695415102387 27.823272758729622 22.32704591905732 54-55 22.807773994372525 26.879752868626046 27.824865021131767 22.487608115869662 56-57 22.714222944348094 27.185785131659458 27.75452769728386 22.34546422670859 58-59 22.774173184545653 26.890517974617982 27.864866855137876 22.47044198569849 60-61 22.794595115477144 26.86252136691301 28.031473558684684 22.311409958925154 62-63 22.696686796711187 26.92032767649789 28.07668963840658 22.306295888384337 64-65 22.74844267781396 26.879729968340264 27.951424961777 22.420402392068777 66-67 22.627660293815303 27.184992275870908 27.6990034326725 22.488343997641287 68-69 22.620107443518787 26.947605406778624 27.94059836359973 22.491688786102863 70-71 22.692964813252882 26.841142648775033 27.877530092001358 22.588362445970727 72-73 22.680435795500898 26.895956260577197 27.738217189273417 22.68539075464849 74-75 22.629551001189462 27.092437388872693 27.502658842756656 22.77535276718119 76-77 22.712136583859536 27.10721272324007 27.586283359057145 22.594367333843245 78-79 22.67880368381156 27.09678231801113 27.529376401475343 22.69503759670197 80-81 22.64473307791598 27.233156621756464 27.47165565470202 22.650454645625533 82-83 22.695484191027298 27.25999891582474 27.516970354377467 22.52754653877049 84-85 22.734780288779668 27.12525402574164 27.32863888196095 22.811326803517744 86-87 22.695343202139515 27.024966402384585 27.49657864168995 22.783111753785942 88-89 22.756893012733435 27.065768531430628 27.36309378132783 22.814244674508103 90-91 22.64296447490927 27.16802043755576 27.463622568803874 22.7253925187311 92-93 22.60291822935202 26.962605053519123 27.477127761504562 22.957348955624298 94-95 22.632501356454284 26.86964843880461 27.466460949173072 23.031389255568033 96-97 22.668318711405103 26.912268704383184 27.44344342919372 22.97596915501799 98-99 22.452890983687563 26.854103514043935 27.35404096026754 23.33896454200096 100-101 22.37942782368505 27.08580454680466 27.25501280491227 23.279754824598022 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 538.0 1 397.5 2 302.0 3 333.0 4 281.5 5 268.0 6 439.0 7 1029.0 8 1816.5 9 2154.0 10 1920.5 11 1677.0 12 1724.0 13 1863.5 14 2023.0 15 2181.5 16 2327.0 17 2485.5 18 2748.5 19 3028.0 20 3318.5 21 3892.0 22 4659.0 23 5606.5 24 6925.5 25 8569.0 26 10893.5 27 13705.0 28 15972.0 29 18332.0 30 21027.0 31 24014.5 32 27420.5 33 30383.5 34 33794.5 35 37580.5 36 41994.0 37 46271.0 38 48481.5 39 49531.5 40 51472.5 41 54578.5 42 56524.0 43 58501.0 44 61396.5 45 64036.5 46 65163.5 47 66031.0 48 68633.5 49 69440.5 50 67936.5 51 66716.5 52 65639.5 53 64941.5 54 63736.0 55 59892.5 56 55083.5 57 50095.5 58 45402.5 59 40536.0 60 34598.5 61 28477.0 62 22467.0 63 17038.5 64 12507.5 65 9272.5 66 6849.5 67 5348.5 68 4026.5 69 2785.0 70 1954.0 71 1252.5 72 842.0 73 552.5 74 390.5 75 279.0 76 181.5 77 95.0 78 53.0 79 31.5 80 18.0 81 11.5 82 8.0 83 8.0 84 4.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02825147978075538 2 0.005310840191343549 3 1.0950185961533091E-4 4 4.3800743846132364E-4 5 5.4750929807665455E-5 6 0.0 7 0.0 8 0.0 9 5.4750929807665455E-5 10-11 0.01084068410191776 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.3800743846132364E-4 20-21 8.760148769226473E-4 22-23 0.0019710334730759565 24-25 0.011306067005282918 26-27 0.005475092980766546 28-29 3.5588104374982543E-4 30-31 0.0024911673062487784 32-33 0.005173962866824386 34-35 0.0 36-37 0.008459018655284313 38-39 0.013769858846627861 40-41 0.0015604014995184655 42-43 0.0 44-45 0.0 46-47 4.106319735574909E-4 48-49 0.0025185427711526113 50-51 0.006241605998073862 52-53 0.0 54-55 0.0030660520692292656 56-57 0.0030386766043254327 58-59 1.3687732451916364E-4 60-61 0.0016972788240376293 62-63 0.004297947989901739 64-65 2.7375464903832728E-5 66-67 0.001834156148556793 68-69 2.737546490383273E-4 70-71 6.843866225958183E-4 72-73 0.0 74-75 2.7375464903832728E-5 76-77 0.0 78-79 0.001615152429326131 80-81 0.0016699033591337964 82-83 0.009855167365379782 84-85 0.006159479603362364 86-87 0.003202929393748429 88-89 1.0950185961533091E-4 90-91 6.296356927881528E-4 92-93 1.6425278942299639E-4 94-95 0.0 96-97 0.001615152429326131 98-99 0.0013413977802878038 100-101 3.0113011394216005E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1826453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.72915429850342 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42260190850344 49.02690047998622 2 12.224519364674945 15.092184841741254 3 3.6650879729185397 6.7872834299363305 4 1.5516457294186932 3.8312711459160163 5 0.8448837548730416 2.607697983442846 6 0.49964301160611996 1.850552433456187 7 0.3409653288962176 1.473325097851221 8 0.24600137916395334 1.2148365673645058 9 0.1688152503746608 0.9378740374486133 >10 0.9475887370158556 10.592624944395965 >50 0.06103167574817626 2.5487655418140847 >100 0.026326735367099702 2.734060616695322 >500 4.445757195773151E-4 0.16762357373158251 >1k 2.6674543174638906E-4 0.3781684423741334 >5k 1.7783028783092603E-4 0.7568308638457076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7676 0.42026813720364004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6113 0.33469243391425896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4104 0.22469781593065902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.0950185961533092E-4 14-15 0.0 0.0 0.0 0.0 1.6425278942299639E-4 16-17 0.0 0.0 0.0 5.475092980766546E-5 3.2850557884599277E-4 18-19 0.0 0.0 0.0 5.475092980766546E-5 3.832565086536582E-4 20-21 0.0 0.0 0.0 1.3687732451916364E-4 4.653829033651564E-4 22-23 0.0 0.0 0.0 3.285055788459927E-4 4.927583682689891E-4 24-25 0.0 0.0 0.0 7.391375524034837E-4 4.927583682689891E-4 26-27 0.0 0.0 0.0 9.307658067303128E-4 4.927583682689891E-4 28-29 0.0 0.0 0.0 0.0016972788240376293 4.927583682689891E-4 30-31 0.0 0.0 0.0 0.0035861859024020875 4.927583682689891E-4 32-33 0.0 0.0 0.0 0.005529843910574211 4.927583682689891E-4 34-35 0.0 0.0 0.0 0.007336624594227171 4.927583682689891E-4 36-37 5.475092980766546E-5 0.0 0.0 0.009773040970668286 4.927583682689891E-4 38-39 1.0950185961533092E-4 0.0 0.0 0.013359226873070371 5.201338331728218E-4 40-41 1.3687732451916364E-4 0.0 0.0 0.01697278824037629 5.475092980766546E-4 42-43 2.4637918413449456E-4 0.0 0.0 0.020586349607682214 5.475092980766546E-4 44-45 3.2850557884599277E-4 0.0 0.0 0.022009873782681516 5.475092980766546E-4 46-47 3.2850557884599277E-4 0.0 0.0 0.024610542948545623 5.475092980766546E-4 48-49 3.2850557884599277E-4 0.0 0.0 0.02767659501777489 6.0226022788432E-4 50-51 3.2850557884599277E-4 0.0 0.0 0.031098528130753977 6.0226022788432E-4 52-53 3.2850557884599277E-4 0.0 0.0 0.03870890737401948 6.296356927881527E-4 54-55 3.2850557884599277E-4 0.0 0.0 0.0439102457057477 7.665130173073164E-4 56-57 3.2850557884599277E-4 0.0 0.0 0.05757060269276023 7.665130173073164E-4 58-59 3.2850557884599277E-4 0.0 0.0 0.08327616423745916 7.665130173073164E-4 60-61 3.2850557884599277E-4 0.0 0.0 0.10451952500283336 7.665130173073164E-4 62-63 3.2850557884599277E-4 0.0 0.0 0.11612672212205843 7.665130173073164E-4 64-65 3.2850557884599277E-4 0.0 0.0 0.1427904249383915 7.665130173073164E-4 66-67 3.2850557884599277E-4 0.0 0.0 0.19135449967779078 7.665130173073164E-4 68-69 3.2850557884599277E-4 0.0 0.0 0.2618189463402562 7.665130173073164E-4 70-71 3.2850557884599277E-4 0.0 0.0 0.3225377274969572 8.486394120188147E-4 72-73 3.2850557884599277E-4 0.0 0.0 0.39201665742288466 8.760148769226474E-4 74-75 3.2850557884599277E-4 0.0 0.0 0.475593951774286 9.033903418264801E-4 76-77 3.2850557884599277E-4 2.737546490383273E-5 0.0 0.5284559745035871 9.581412716341455E-4 78-79 3.2850557884599277E-4 5.475092980766546E-5 0.0 0.5912826664578832 9.855167365379783E-4 80-81 3.2850557884599277E-4 5.475092980766546E-5 0.0 0.6852078865429332 0.001012892201441811 82-83 3.832565086536582E-4 5.475092980766546E-5 0.0 0.7696611957712571 0.0010402676663456437 84-85 4.10631973557491E-4 5.475092980766546E-5 0.0 0.8848297766216815 0.0010402676663456437 86-87 4.653829033651564E-4 5.475092980766546E-5 0.0 1.064275949066305 0.0010676431312494764 88-89 4.927583682689891E-4 5.475092980766546E-5 0.0 1.2874133634974456 0.0010950185961533091 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4245 0.0 48.131794 1 GTATCAA 7290 0.0 40.607086 1 ATCAACG 9340 0.0 31.32627 3 TATCAAC 9375 0.0 31.261694 2 TCAACGC 9440 0.0 30.944109 4 CAACGCA 9670 0.0 30.208933 5 AACGCAG 9915 0.0 29.510374 6 ACGCAGA 11265 0.0 25.805183 7 CGCAGAG 11225 0.0 25.770191 8 GCAGAGT 12475 0.0 22.654951 9 TGGTATC 2250 0.0 22.58915 2 GTGGTAT 2370 0.0 22.254402 1 GAGTACT 7280 0.0 21.921528 12-13 GTACATG 7115 0.0 20.569187 1 GTACTTT 7745 0.0 20.299036 14-15 AGTACTT 7575 0.0 20.284046 12-13 AGAGTAC 11195 0.0 20.089575 10-11 ACATGGG 6935 0.0 20.067625 3 TACATGG 7255 0.0 19.903744 2 CAGAGTA 11970 0.0 19.642008 10-11 >>END_MODULE