FastQCFastQC Report
Thu 26 May 2016
SRR936864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809952
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.3522745354572939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.2669131557079967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31130.17199351143013739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35850.048.252891
GTATCAA63250.041.0996321
ATCAACG81850.031.2971823
TATCAAC83050.030.9585632
TCAACGC83650.030.6254184
CAACGCA83700.030.4935515
AACGCAG87050.029.4805876
ACGCAGA98950.025.7868087
CGCAGAG98050.025.53968
GCAGAGT107400.022.5634239
GAGTACT56900.021.21126212-13
TACATGG71750.020.9971582
GTACATG72750.020.9039381
ACATGGG69300.020.7113723
AGAGTAC94850.020.4382610-11
GTACTTT60300.020.05550414-15
GTGGTAT19050.019.9574991
AGTACTT60300.019.5424712-13
CAGAGTA101200.019.46099710-11
GTGTAGC9650.017.7290841
Produced by FastQC (version 0.11.5)