##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1809952 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493111419529356 31.0 31.0 34.0 30.0 34.0 2 31.576573301391416 33.0 31.0 34.0 30.0 34.0 3 31.59678322961051 33.0 31.0 34.0 28.0 34.0 4 35.19731683492159 37.0 35.0 37.0 33.0 37.0 5 35.193590769257966 37.0 35.0 37.0 33.0 37.0 6 35.248872898286805 37.0 35.0 37.0 32.0 37.0 7 35.185538622018704 37.0 35.0 37.0 32.0 37.0 8 35.14073688142006 37.0 35.0 37.0 32.0 37.0 9 36.721232938774065 39.0 37.0 39.0 32.0 39.0 10-11 36.57736254884107 39.0 37.0 39.0 32.0 39.0 12-13 36.47369239626244 39.0 37.0 39.0 32.0 39.0 14-15 37.57039247449656 40.0 37.0 41.0 32.0 41.0 16-17 37.4818014510882 40.0 37.0 41.0 32.0 41.0 18-19 37.432229141988294 40.0 37.0 41.0 32.0 41.0 20-21 37.35023801736179 40.0 37.0 41.0 31.5 41.0 22-23 37.22668170205618 40.0 37.0 41.0 31.0 41.0 24-25 37.17668148105585 39.5 36.5 41.0 31.0 41.0 26-27 37.20214154850515 40.0 37.0 41.0 31.0 41.0 28-29 37.12612682546278 40.0 37.0 41.0 31.0 41.0 30-31 36.96216391373915 39.0 36.0 41.0 30.0 41.0 32-33 36.782554730733196 39.0 36.0 41.0 30.0 41.0 34-35 36.61746775605099 39.0 35.5 41.0 30.0 41.0 36-37 36.43131834435388 39.0 35.0 41.0 30.0 41.0 38-39 36.26261773792896 39.0 35.0 41.0 30.0 41.0 40-41 36.07388510855536 39.0 35.0 40.5 29.0 41.0 42-43 35.848478854687855 39.0 35.0 40.0 28.0 41.0 44-45 35.603597222467776 38.5 35.0 40.0 27.0 41.0 46-47 35.27980300030056 38.0 34.5 40.0 26.0 41.0 48-49 35.04237626191192 38.0 34.0 40.0 25.0 41.0 50-51 34.342749973479954 37.5 33.5 39.5 24.5 40.5 52-53 34.52755404563215 38.0 33.5 39.5 24.5 40.5 54-55 34.91337781333428 38.0 34.0 40.0 26.0 41.0 56-57 34.77704767861247 38.0 34.0 40.0 25.0 41.0 58-59 34.543524082406606 38.0 34.0 40.0 24.0 41.0 60-61 34.24541866303637 38.0 34.0 40.0 23.5 41.0 62-63 33.914106838192396 37.0 33.0 40.0 23.0 41.0 64-65 33.56438900037128 37.0 33.0 40.0 22.0 41.0 66-67 33.1847084895069 36.0 32.5 39.0 21.5 41.0 68-69 32.78496446314598 36.0 32.0 39.0 20.5 41.0 70-71 32.37217616820777 35.0 32.0 38.5 19.5 40.0 72-73 31.97211307261187 35.0 31.0 37.5 18.0 40.0 74-75 31.567090176977068 35.0 31.0 37.0 17.5 39.0 76-77 31.109413675058786 35.0 31.0 36.5 12.5 39.0 78-79 30.650612833931508 35.0 30.5 36.0 9.0 39.0 80-81 30.225319234985236 34.0 30.0 36.0 7.0 37.5 82-83 29.820986412899348 34.0 30.0 35.0 4.5 37.0 84-85 29.445386673237742 34.0 29.0 35.0 2.0 36.5 86-87 29.08111623954668 34.0 29.0 35.0 2.0 36.0 88-89 28.712706469563834 34.0 29.0 35.0 2.0 36.0 90-91 28.333235080267322 34.0 29.0 35.0 2.0 35.5 92-93 27.930342904121215 34.0 27.5 35.0 2.0 35.0 94-95 27.527092431180495 34.0 27.0 35.0 2.0 35.0 96-97 26.986722852318735 33.0 25.5 35.0 2.0 35.0 98-99 26.351077266137445 33.0 25.0 35.0 2.0 35.0 100-101 24.089941611711247 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39000856712533505 1101 2 0.42317331161909877 1101 3 0.5397861022027506 1101 4 0.41814463489139797 1101 5 0.32769146449037123 1101 6 0.3414298702658627 1101 7 0.3687037149014998 1101 8 0.4130216956571644 1101 9 0.4814848694555849 1101 10-11 0.44824928473664016 1101 12-13 0.7072864117000037 1101 14-15 0.8778875824763688 1101 16-17 0.731533773558148 1101 18-19 0.766020855200324 1101 20-21 0.7492407671450323 1101 22-23 0.6861453265387425 1101 24-25 0.7473783014867976 1101 26-27 0.628461725830121 1101 28-29 0.8149527504776302 1101 30-31 0.7520388900903967 1101 32-33 0.6282525405869563 1101 34-35 0.7604401074154978 1101 36-37 0.7124692341073668 1101 38-39 0.736226113443692 1101 40-41 0.6142889577132422 1101 42-43 0.5674656794271655 1101 44-45 0.7367819683734567 1101 46-47 0.7738796232939436 1101 48-49 0.8424009935761774 1101 50-51 0.8029712912552824 1101 52-53 0.803407155178796 1101 54-55 0.9830994437281007 1101 56-57 0.9764251011386165 1101 58-59 0.8874991604081117 1101 60-61 1.0564466844618536 1101 62-63 0.9785618738603858 1101 64-65 1.0362081358662536 1101 66-67 0.9188534796290924 1101 68-69 0.9061229151605232 1101 70-71 0.8964298665658674 1101 72-73 0.9159274027716151 1101 74-75 0.9648441766899687 1101 76-77 0.9569270557893823 1101 78-79 1.045769423823259 1101 80-81 0.92814117509322 1101 82-83 0.8410539668040968 1101 84-85 0.7721015273039988 1101 86-87 0.8961622315030731 1101 88-89 1.0804608967453362 1101 90-91 0.8698870689267544 1101 92-93 1.0588485246596484 1101 94-95 0.9658122486231662 1101 96-97 1.1378643010735097 1101 98-99 1.0633821033667026 1101 100-101 1.2869256988153381 1102 1 0.39630132971012344 1102 2 0.2776395193954251 1102 3 0.3102818750372691 1102 4 0.2950031555163619 1102 5 0.440217044525987 1102 6 0.26670811313519494 1102 7 0.42145838574955974 1102 8 0.17112979179380972 1102 9 0.43162705458860273 1102 10-11 0.45593879847235286 1102 12-13 0.2230596596336767 1102 14-15 0.23304035397909928 1102 16-17 0.45781041296896774 1102 18-19 0.35114589838740073 1102 20-21 0.29570415188943855 1102 22-23 0.3864019389594944 1102 24-25 0.22597670747776277 1102 26-27 0.38986828081575453 1102 28-29 0.29662223722326075 1102 30-31 0.4347106122284572 1102 32-33 0.3653901752051283 1102 34-35 0.2135204900012937 1102 36-37 0.2214418099468176 1102 38-39 0.2969862649897195 1102 40-41 0.20115329567386908 1102 42-43 0.23447046027306584 1102 44-45 0.3116956680512928 1102 46-47 0.2404137019549495 1102 48-49 0.3837232710187948 1102 50-51 0.3228476802549167 1102 52-53 0.49062348624129726 1102 54-55 0.2524745673833948 1102 56-57 0.46835170230857415 1102 58-59 0.26827773843866254 1102 60-61 0.2100893110627311 1102 62-63 0.1940040947242423 1102 64-65 0.39885109615186565 1102 66-67 0.11689979979517062 1102 68-69 0.3565057667180582 1102 70-71 0.24091949133295287 1102 72-73 0.17148561599321255 1102 74-75 0.44501503508657336 1102 76-77 0.17390038758717452 1102 78-79 0.14247303204784956 1102 80-81 0.17860848137151208 1102 82-83 0.4136171085197091 1102 84-85 0.2955229475016665 1102 86-87 0.5364796158872167 1102 88-89 0.42693934159728997 1102 90-91 0.18971945878864105 1102 92-93 0.166170985185925 1102 94-95 0.4310721240672173 1102 96-97 0.15371727898830656 1102 98-99 0.27153346040051574 1102 100-101 0.031910830650339506 1103 1 0.3387786117413931 1103 2 0.3742387619000418 1103 3 0.26037007368111276 1103 4 0.28126538509896193 1103 5 0.35585083104990645 1103 6 0.3334348975319159 1103 7 0.29806419744979706 1103 8 0.43630742080567586 1103 9 0.362257307772353 1103 10-11 0.47634451223403573 1103 12-13 0.3351936112865488 1103 14-15 0.6406970032218169 1103 16-17 0.6443109856757658 1103 18-19 0.6047961174904515 1103 20-21 0.5728560279761865 1103 22-23 0.6254382451535321 1103 24-25 0.47174029555323926 1103 26-27 0.5533321649245977 1103 28-29 0.6675327481160735 1103 30-31 0.5261704780993881 1103 32-33 0.5025444607961091 1103 34-35 0.5542988617638187 1103 36-37 0.49890176692338173 1103 38-39 0.5625596340939936 1103 40-41 0.5254719663947398 1103 42-43 0.6022023136193653 1103 44-45 0.5715911183438749 1103 46-47 0.5376492753480306 1103 48-49 0.5187559042173291 1103 50-51 0.6114616339217065 1103 52-53 0.5693263158503825 1103 54-55 0.6559960997801397 1103 56-57 0.7555292438110897 1103 58-59 0.6993485353072657 1103 60-61 0.7490074496547763 1103 62-63 0.8475998002924143 1103 64-65 0.9144105296906062 1103 66-67 0.9251134520941164 1103 68-69 0.8367049763766943 1103 70-71 0.7415725149311143 1103 72-73 0.6629564505028256 1103 74-75 0.6521291562798446 1103 76-77 0.7103766554689024 1103 78-79 0.8139164509545473 1103 80-81 0.9077216159866772 1103 82-83 0.9137374338866948 1103 84-85 0.8498583169260172 1103 86-87 0.9160099483688349 1103 88-89 0.7075166696009418 1103 90-91 0.8156720970459368 1103 92-93 0.7367727558718471 1103 94-95 0.9268725739612158 1103 96-97 0.9660808827675105 1103 98-99 1.078383225413912 1103 100-101 1.1661416443734822 1104 1 0.27334107915129024 1104 2 0.21747703356718873 1104 3 0.19283828187505136 1104 4 0.21648206081489718 1104 5 0.3077842133379889 1104 6 0.14375327135860516 1104 7 0.1911782799417594 1104 8 0.260432392426452 1104 9 0.2676204570762195 1104 10-11 0.43626300087979786 1104 12-13 0.2691426345600547 1104 14-15 0.06401365532850178 1104 16-17 0.4533986495887703 1104 18-19 0.42457192021905144 1104 20-21 0.4836669971613077 1104 22-23 0.1812585476349966 1104 24-25 0.25288044106999763 1104 26-27 0.23857502595784297 1104 28-29 0.2735450668216828 1104 30-31 0.54505532894499 1104 32-33 0.15809048269439074 1104 34-35 0.08622810535634073 1104 36-37 0.14873004785871302 1104 38-39 0.28885130625722155 1104 40-41 0.3027659841962205 1104 42-43 0.3801959747909862 1104 44-45 0.14561870889270523 1104 46-47 0.037500231352730395 1104 48-49 0.07088591700708946 1104 50-51 0.17633158206628963 1104 52-53 0.2241204652861697 1104 54-55 0.4154198416251802 1104 56-57 0.39341602925624386 1104 58-59 0.4621950519806646 1104 60-61 0.3263072140718464 1104 62-63 0.17199200369203282 1104 64-65 0.14989055892757364 1104 66-67 0.0394804439871379 1104 68-69 0.033459185984924034 1104 70-71 0.10632209554140815 1104 72-73 0.16762479718170198 1104 74-75 0.21032241467881718 1104 76-77 0.22434163904717153 1104 78-79 0.26591947203245425 1104 80-81 0.08285425523453327 1104 82-83 0.1464112463613212 1104 84-85 0.08672036474805012 1104 86-87 0.24103555336289872 1104 88-89 0.18201570909519305 1104 90-91 0.23913198991543183 1104 92-93 0.3309994171108386 1104 94-95 0.45687471974181193 1104 96-97 0.5855443866630345 1104 98-99 0.5867475234747275 1104 100-101 0.7385197014738978 1105 1 0.2584879473257615 1105 2 0.3454667092178134 1105 3 0.4385623252319526 1105 4 0.26577317023522085 1105 5 0.3157988892541539 1105 6 0.2750695140959749 1105 7 0.35241853238182586 1105 8 0.3172956976605761 1105 9 0.5984798523850898 1105 10-11 0.4462911988537215 1105 12-13 0.49793908868160486 1105 14-15 0.6487695589298923 1105 16-17 0.5602835896680745 1105 18-19 0.47806365565228504 1105 20-21 0.5174822042772149 1105 22-23 0.6089017303387507 1105 24-25 0.5381829739121997 1105 26-27 0.6164150186679649 1105 28-29 0.5911274036197511 1105 30-31 0.6768814160959593 1105 32-33 0.6355416072311542 1105 34-35 0.4451856859657042 1105 36-37 0.30021714996903626 1105 38-39 0.672004833715306 1105 40-41 0.6706408106842048 1105 42-43 0.8986284995103375 1105 44-45 0.6316336263141196 1105 46-47 0.6571365926512911 1105 48-49 0.7581732372547805 1105 50-51 0.620720666029051 1105 52-53 0.8077138453589683 1105 54-55 0.7545773460933063 1105 56-57 0.872788014982838 1105 58-59 0.7685814961568909 1105 60-61 0.6908018056400635 1105 62-63 0.7389198444953493 1105 64-65 0.7137385090120105 1105 66-67 0.634084674465889 1105 68-69 0.7478399570239773 1105 70-71 0.8750731332592707 1105 72-73 0.9902597368417361 1105 74-75 0.9722246542486666 1105 76-77 0.8490340972072268 1105 78-79 0.9188133981195499 1105 80-81 0.7461397552482332 1105 82-83 0.6380826695260353 1105 84-85 0.8067668656891946 1105 86-87 1.014986500963527 1105 88-89 1.063106099701983 1105 90-91 1.0751137283171737 1105 92-93 0.9583653439627007 1105 94-95 0.9958089157110237 1105 96-97 0.8980229684458259 1105 98-99 1.0859598362173166 1105 100-101 1.1648313908310612 1106 1 0.30592038835929003 1106 2 0.3035042102177883 1106 3 0.2287218017057988 1106 4 0.3524000013592641 1106 5 0.2791910065815628 1106 6 0.42939983377883095 1106 7 0.3650520985271086 1106 8 0.42072796585152616 1106 9 0.4230434686722333 1106 10-11 0.511424675950046 1106 12-13 0.5991776867648042 1106 14-15 0.46546664822586337 1106 16-17 0.57095442908156 1106 18-19 0.6063131398982051 1106 20-21 0.5414521413473352 1106 22-23 0.4613703719654296 1106 24-25 0.385430280692006 1106 26-27 0.3006412649300927 1106 28-29 0.24418665884342516 1106 30-31 0.41437120466160593 1106 32-33 0.3967114128007765 1106 34-35 0.5133028872473133 1106 36-37 0.42537400752748056 1106 38-39 0.32006712828226824 1106 40-41 0.49746685455702533 1106 42-43 0.3618014979962112 1106 44-45 0.2555902206379912 1106 46-47 0.2882025058177575 1106 48-49 0.38877069448354007 1106 50-51 0.38020686227194034 1106 52-53 0.4651674479296517 1106 54-55 0.42057716741145157 1106 56-57 0.4059629871440862 1106 58-59 0.4401217687693375 1106 60-61 0.4627293098574796 1106 62-63 0.5329257982214202 1106 64-65 0.30307622413824475 1106 66-67 0.36547869656521215 1106 68-69 0.425592051718354 1106 70-71 0.5121870768086509 1106 72-73 0.6668308379131815 1106 74-75 0.825968155864107 1106 76-77 0.7822234339880687 1106 78-79 0.6271965842739213 1106 80-81 0.6293729157973793 1106 82-83 0.6832915141609952 1106 84-85 0.713815514451472 1106 86-87 0.8165341719389971 1106 88-89 0.7332761861407882 1106 90-91 0.8174426710986893 1106 92-93 0.8195098362697486 1106 94-95 0.9303998247753924 1106 96-97 1.1825310473764894 1106 98-99 1.200751832438133 1106 100-101 1.4722136666422685 1107 1 0.29904219648209107 1107 2 0.3411511348397731 1107 3 0.37302355424196065 1107 4 0.3610361246611049 1107 5 0.37069623921767203 1107 6 0.33105309706621 1107 7 0.3886885994974847 1107 8 0.4016213292133628 1107 9 0.5055515577156839 1107 10-11 0.4559078635341507 1107 12-13 0.5922261192862379 1107 14-15 0.6010330045877268 1107 16-17 0.6770678807099202 1107 18-19 0.6653919061398881 1107 20-21 0.5992935421430587 1107 22-23 0.6015216243605437 1107 24-25 0.6087379788099838 1107 26-27 0.7339377563123151 1107 28-29 0.7103975027090854 1107 30-31 0.8129747559790559 1107 32-33 0.7418697792379092 1107 34-35 0.6841272991249028 1107 36-37 0.684113026236723 1107 38-39 0.8342725522190548 1107 40-41 0.887469428869089 1107 42-43 0.9805010233670615 1107 44-45 0.9611350113556725 1107 46-47 0.9257333551711682 1107 48-49 1.0372377456013666 1107 50-51 0.9548509674826917 1107 52-53 1.0299627284602977 1107 54-55 1.037298480403102 1107 56-57 1.031057134514974 1107 58-59 1.1073755943974675 1107 60-61 1.12762879607412 1107 62-63 1.0998525166518007 1107 64-65 1.1863986898667562 1107 66-67 1.1427142245381177 1107 68-69 1.0538450495587028 1107 70-71 1.0638874330502759 1107 72-73 1.1196003814891924 1107 74-75 1.1166292607835153 1107 76-77 1.018614340962909 1107 78-79 1.0455557455106401 1107 80-81 1.1086352135327537 1107 82-83 1.1397333832657068 1107 84-85 1.1947264509615358 1107 86-87 1.1883784430155977 1107 88-89 1.12517558140868 1107 90-91 1.0421801143906002 1107 92-93 1.1856907749533327 1107 94-95 1.3067263277034797 1107 96-97 1.1665729331294479 1107 98-99 1.4425808580378963 1107 100-101 1.2688262110953943 1108 1 0.0641501116677965 1108 2 0.1496336886568237 1108 3 0.18247950205027763 1108 4 0.13425865467034015 1108 5 0.08874506632429302 1108 6 0.1310893569059104 1108 7 0.07925274919721659 1108 8 0.21037653453822003 1108 9 0.10526794393076244 1108 10-11 -0.06665531361484511 1108 12-13 0.16162829571986492 1108 14-15 0.31194848121488405 1108 16-17 0.2512595884499902 1108 18-19 0.29079234547422317 1108 20-21 0.29774645564928903 1108 22-23 0.13909131563190158 1108 24-25 0.11889354476564762 1108 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2316 50-51 -2.223490702525254 2316 52-53 -2.0701166998994367 2316 54-55 -2.0296402652386334 2316 56-57 -2.3443191592449395 2316 58-59 -2.2743895751233865 2316 60-61 -2.4506669853804937 2316 62-63 -2.4812421616480975 2316 64-65 -3.0305822092134918 2316 66-67 -2.556906928103068 2316 68-69 -2.6206425917849003 2316 70-71 -2.255810800164191 2316 72-73 -2.6510708872454565 2316 74-75 -3.1530586818085418 2316 76-77 -3.3057160444247096 2316 78-79 -3.522723220304673 2316 80-81 -3.3050403376253605 2316 82-83 -3.9481372506518824 2316 84-85 -3.692150032603198 2316 86-87 -4.0099945714659775 2316 88-89 -4.007535029518895 2316 90-91 -4.599658202398967 2316 92-93 -4.632118404891681 2316 94-95 -4.653984685701772 2316 96-97 -4.820089200581567 2316 98-99 -5.197567662323053 2316 100-101 -5.3249992629973875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4516.0 3 1744.0 4 1783.0 5 1943.0 6 2206.0 7 2593.0 8 3143.0 9 4001.0 10 4849.0 11 5968.0 12 7181.0 13 8559.0 14 9312.0 15 11290.0 16 13173.0 17 16211.0 18 14959.0 19 11242.0 20 11277.0 21 12482.0 22 13170.0 23 14947.0 24 17008.0 25 19583.0 26 22846.0 27 26818.0 28 31499.0 29 37450.0 30 45702.0 31 55980.0 32 70549.0 33 88838.0 34 119819.0 35 166731.0 36 245068.0 37 345080.0 38 290766.0 39 49632.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65947996648368 18.788095517082045 14.497244189362771 23.0551803270715 2 15.326764804060492 20.106596751695 40.237952599594564 24.328685844649936 3 19.156254507453152 24.52872418559129 29.594092963648027 26.720928343307527 4 12.959736536928718 16.77537429510473 37.28242352418339 32.98246564378316 5 14.162275334195654 36.899967206714386 34.037329044952244 14.900428414137723 6 31.665002724072227 36.127392551252655 17.73733306415259 14.470271660522524 7 28.38035355551158 32.52217599497606 20.92437662318996 18.173093826322408 8 26.21454305155791 34.74229341030937 19.92772436827946 19.115439169853264 9 26.19893432974295 16.95894178291046 20.474205018502985 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22.563441644461573 26.60489299424118 28.70105382680441 22.130611534492832 36-37 22.578775457656974 26.455906270348788 28.608302320062478 22.357015951931754 38-39 22.362184842376987 26.71325518485122 28.665724066903586 22.258835905868207 40-41 22.584256283979546 26.519757069830955 28.729617108436994 22.1663695377525 42-43 22.428950219709122 26.579223191647237 28.515525503441363 22.476301085202273 44-45 22.520773143843144 26.77427428671828 28.34493336405311 22.360019205385466 46-47 22.67083773843855 26.88896482812976 27.941466131359476 22.498731302072212 48-49 22.672321344216158 27.010901424185352 27.784294236240065 22.53248299535843 50-51 22.626676564003436 27.146061319471173 27.68404253350089 22.543219583024502 52-53 22.808774020729974 27.182413060138366 27.531725127538685 22.47708779159297 54-55 22.694324567753203 27.14907748669691 27.52416106269996 22.632436882849927 56-57 22.654641788459355 27.411649938791697 27.47021983325814 22.463488439490806 58-59 22.677057893980752 27.14798382195308 27.55425834042412 22.62069994364205 60-61 22.69477625072759 27.086608691616437 27.779071575953918 22.439543481702056 62-63 22.621751156758933 27.125789729019484 27.802163556374797 22.450295557846786 64-65 22.59813134199878 27.117531079609826 27.689935706694563 22.594401871696828 66-67 22.568390338521986 27.44834027103311 27.37848893997566 22.604780450469246 68-69 22.473722379202542 27.226716060192004 27.692488823443572 22.607072737161886 70-71 22.577284508767697 27.127077488916512 27.550570531175428 22.745067471140363 72-73 22.58679328213131 27.187350387965676 27.484799486377483 22.74105684352553 74-75 22.478501300628142 27.429922874063557 27.31133984729534 22.78023597801296 76-77 22.64108923475489 27.341669042929677 27.349875439608656 22.667366282706773 78-79 22.582436757522075 27.37068626077528 27.33628493910137 22.71059204260127 80-81 22.499104974407107 27.470028737578495 27.289475466622804 22.741390821391597 82-83 22.564172566687134 27.515243523333243 27.29965398459317 22.620929925386456 84-85 22.59952220170691 27.42090868980105 27.078957176354095 22.90061193213794 86-87 22.581895796569366 27.33650270282519 27.253630064723005 22.827971435882443 88-89 22.657062068664363 27.345743141675282 27.15255673503365 22.844638054626703 90-91 22.60426615727475 27.37716226254908 27.28900252927235 22.729569050903816 92-93 22.555746827998785 27.24860347932834 27.304594195813298 22.891055496859572 94-95 22.546137613558564 27.179321552325764 27.26339398446967 23.011146849646 96-97 22.55594703529617 27.175770228407796 27.308170936165837 22.96011180013019 98-99 22.398813899374893 27.196635144172575 27.21399001818393 23.190560938268604 100-101 22.43733924278514 27.264091897283464 27.16758227783712 23.130986582094273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 350.0 1 259.5 2 203.5 3 263.0 4 263.5 5 274.5 6 447.0 7 953.5 8 1620.0 9 1924.5 10 1765.0 11 1566.0 12 1591.0 13 1721.0 14 1856.5 15 1980.0 16 2113.0 17 2288.5 18 2471.5 19 2745.0 20 3120.5 21 3567.0 22 4380.5 23 5449.0 24 6554.0 25 8180.5 26 10604.0 27 13397.0 28 16004.0 29 18256.0 30 20626.0 31 23727.0 32 27166.5 33 30493.5 34 34020.0 35 37573.0 36 41833.5 37 45830.5 38 48064.0 39 49628.5 40 51536.0 41 54046.0 42 56202.5 43 58668.0 44 61531.0 45 63738.5 46 64819.0 47 65893.5 48 68159.5 49 68443.0 50 67172.5 51 66080.5 52 64820.5 53 64344.0 54 62921.0 55 59068.5 56 54449.0 57 49352.5 58 44856.5 59 40205.5 60 34253.0 61 28376.5 62 22391.5 63 16856.0 64 12554.5 65 9431.5 66 6915.5 67 5322.5 68 3989.5 69 2726.5 70 1951.5 71 1273.5 72 859.5 73 606.0 74 416.0 75 286.5 76 178.5 77 92.5 78 52.5 79 31.0 80 24.5 81 23.0 82 14.5 83 10.0 84 10.0 85 7.5 86 5.5 87 5.0 88 4.5 89 3.5 90 2.5 91 3.0 92 2.0 93 1.5 94 2.0 95 0.5 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03779105744240731 2 0.02850904333374587 3 0.024807287707077318 4 0.04519456869574442 5 0.09143888898711126 6 0.11033441770831491 7 0.05602358515584944 8 0.08823438411626386 9 0.07320636127366914 10-11 0.07124498329237461 12-13 0.07168698396421562 14-15 0.06442159792082884 16-17 0.052404704655151076 18-19 0.06049884195823978 20-21 0.055913084987889175 22-23 0.03298430013613621 24-25 0.009061013772740933 26-27 0.0026243789890560633 28-29 0.0066576351196053814 30-31 0.004309506550449957 32-33 0.006381384699704743 34-35 0.006602385035625253 36-37 0.00599463411184385 38-39 0.0035360053747281696 40-41 0.007265386043386786 42-43 0.0032597549548275314 44-45 0.002375753611145489 46-47 0.001408877141493255 48-49 2.762504199006382E-4 50-51 0.002071878149254787 52-53 0.006022259153833914 54-55 0.012265518643588338 56-57 0.008204637471048956 58-59 0.005083007726171744 60-61 0.0019613779812945316 62-63 0.008508512932939659 64-65 0.002403378653135553 66-67 0.021796158130160358 68-69 0.04464206785594314 70-71 0.05897946464878626 72-73 0.003453130248757978 74-75 0.013784895953041849 76-77 0.021437032584289528 78-79 0.023674660985484697 80-81 0.07428373791128162 82-83 0.027348791570163185 84-85 0.010635641166174574 86-87 0.030332296105090077 88-89 0.04522219373773448 90-91 0.04066406180937395 92-93 0.03997343575962235 94-95 0.011215767047965912 96-97 0.01624352469015753 98-99 0.0365755555948445 100-101 0.073151111189689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1809952.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.03759521366254 #Duplication Level Percentage of deduplicated Percentage of total 1 80.89936852780151 51.80601014824264 2 11.497528562033258 14.725481600260187 3 3.342849294094399 6.422040898664839 4 1.4011892056413948 3.5891514867446803 5 0.7524859769972008 2.409369619945206 6 0.4457236682904079 1.712584310827796 7 0.3225075421885695 1.4456825208017363 8 0.22481724424075972 1.1517404546992702 9 0.16173494245602918 0.9321405099213781 >10 0.8746923051632935 10.096137020275231 >50 0.05361521297377389 2.3561022352086134 >100 0.022883258526896457 2.388276518307953 >500 2.5896839680752357E-4 0.10342709237108227 >1k 2.5896839680752346E-4 0.5093963956170618 >5k 8.632279893584114E-5 0.3524591881122503 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6376 0.3522745354572939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4831 0.2669131557079967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3113 0.17199351143013739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.525008398012765E-5 0.0 5 0.0 0.0 0.0 5.525008398012765E-5 0.0 6 0.0 0.0 0.0 5.525008398012765E-5 0.0 7 0.0 0.0 0.0 5.525008398012765E-5 0.0 8 0.0 0.0 0.0 5.525008398012765E-5 0.0 9 0.0 0.0 0.0 5.525008398012765E-5 0.0 10-11 0.0 0.0 0.0 5.525008398012765E-5 0.0 12-13 0.0 0.0 0.0 1.105001679602553E-4 1.105001679602553E-4 14-15 0.0 0.0 0.0 1.105001679602553E-4 1.6575025194038296E-4 16-17 0.0 0.0 0.0 1.105001679602553E-4 1.6575025194038296E-4 18-19 0.0 0.0 0.0 1.105001679602553E-4 2.210003359205106E-4 20-21 0.0 0.0 0.0 1.105001679602553E-4 2.7625041990063826E-4 22-23 0.0 0.0 0.0 3.867505878608936E-4 2.7625041990063826E-4 24-25 0.0 0.0 0.0 6.35375965771468E-4 2.7625041990063826E-4 26-27 0.0 0.0 0.0 9.392514276621701E-4 3.038754618907021E-4 28-29 8.287512597019147E-5 0.0 0.0 0.0017956277293541486 3.315005038807659E-4 30-31 1.105001679602553E-4 0.0 0.0 0.0037570057106486804 3.315005038807659E-4 32-33 1.105001679602553E-4 0.0 0.0 0.005083007726171744 3.315005038807659E-4 34-35 1.3812520995031913E-4 0.0 0.0 0.006602385035625254 3.315005038807659E-4 36-37 1.6575025194038296E-4 0.0 0.0 0.008978138646770743 3.315005038807659E-4 38-39 1.6575025194038296E-4 0.0 0.0 0.012900894609359805 3.591255458708297E-4 40-41 1.6575025194038296E-4 0.0 0.0 0.01640927494209791 3.8675058786089353E-4 42-43 1.933752939304468E-4 0.0 0.0 0.020470156114637296 4.420006718410212E-4 44-45 2.210003359205106E-4 0.0 0.0 0.02306691006170329 4.972507558211488E-4 46-47 2.210003359205106E-4 0.0 0.0 0.02571891409274942 5.525008398012765E-4 48-49 2.210003359205106E-4 0.0 0.0 0.02925491946747759 6.077509237814041E-4 50-51 2.210003359205106E-4 0.0 0.0 0.03342630080797723 6.077509237814041E-4 52-53 2.210003359205106E-4 0.0 0.0 0.04199006382489701 6.077509237814041E-4 54-55 2.210003359205106E-4 0.0 0.0 0.04652057071126749 6.35375965771468E-4 56-57 2.210003359205106E-4 0.0 0.0 0.06182484397376284 6.630010077615318E-4 58-59 2.210003359205106E-4 0.0 0.0 0.08759900815049239 6.630010077615318E-4 60-61 2.210003359205106E-4 0.0 0.0 0.10715753787945757 6.906260497515956E-4 62-63 2.210003359205106E-4 0.0 0.0 0.1195059316490161 7.182510917416595E-4 64-65 2.210003359205106E-4 0.0 0.0 0.1453905959937059 7.182510917416595E-4 66-67 2.210003359205106E-4 0.0 0.0 0.1949499213238804 7.182510917416595E-4 68-69 2.210003359205106E-4 0.0 0.0 0.26677503049804635 7.182510917416595E-4 70-71 2.486253779105744E-4 0.0 0.0 0.3280473736320079 7.182510917416595E-4 72-73 2.7625041990063826E-4 0.0 0.0 0.40089460935980625 7.182510917416595E-4 74-75 2.7625041990063826E-4 0.0 0.0 0.492112498010997 7.735011757217871E-4 76-77 3.038754618907021E-4 0.0 0.0 0.5436055762804759 8.287512597019148E-4 78-79 3.315005038807659E-4 0.0 0.0 0.60595529605205 8.287512597019148E-4 80-81 3.315005038807659E-4 0.0 0.0 0.6948526811760753 8.840013436820424E-4 82-83 3.8675058786089353E-4 0.0 0.0 0.7761531797528332 9.3925142766217E-4 84-85 3.8675058786089353E-4 0.0 0.0 0.8899407277099061 9.3925142766217E-4 86-87 3.8675058786089353E-4 0.0 0.0 1.0731223811460193 9.3925142766217E-4 88-89 3.8675058786089353E-4 0.0 0.0 1.3053937341984758 9.3925142766217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3585 0.0 48.25289 1 GTATCAA 6325 0.0 41.099632 1 ATCAACG 8185 0.0 31.297182 3 TATCAAC 8305 0.0 30.958563 2 TCAACGC 8365 0.0 30.625418 4 CAACGCA 8370 0.0 30.493551 5 AACGCAG 8705 0.0 29.480587 6 ACGCAGA 9895 0.0 25.786808 7 CGCAGAG 9805 0.0 25.5396 8 GCAGAGT 10740 0.0 22.563423 9 GAGTACT 5690 0.0 21.211262 12-13 TACATGG 7175 0.0 20.997158 2 GTACATG 7275 0.0 20.903938 1 ACATGGG 6930 0.0 20.711372 3 AGAGTAC 9485 0.0 20.43826 10-11 GTACTTT 6030 0.0 20.055504 14-15 GTGGTAT 1905 0.0 19.957499 1 AGTACTT 6030 0.0 19.54247 12-13 CAGAGTA 10120 0.0 19.460997 10-11 GTGTAGC 965 0.0 17.729084 1 >>END_MODULE