FastQCFastQC Report
Thu 26 May 2016
SRR936864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809952
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.4326081575643995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61980.34244002050883116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41440.22895634801364897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40900.051.1089131
GTATCAA71150.040.396871
ATCAACG91100.031.2314173
TCAACGC91550.031.128934
TATCAAC91350.031.0948092
CAACGCA94000.030.4186465
AACGCAG97550.029.218326
ACGCAGA110200.025.734997
CGCAGAG111050.025.496648
GCAGAGT121600.022.9329419
GTACATG67200.021.7745251
GAGTACT74700.021.62138712-13
TACATGG66250.021.2227212
TGGTATC20650.020.7022572
ACATGGG65050.020.5913623
GTACTTT78250.020.48673214-15
GTGGTAT22150.020.3759171
CAGAGTA117000.020.03810110-11
AGAGTAC111050.019.46471610-11
AGTACTT78500.019.45523812-13
Produced by FastQC (version 0.11.5)