FastQCFastQC Report
Thu 26 May 2016
SRR936863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377540
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64650.46931486563003616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.33748566284826576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.2184328585739797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.039.2496451
GTATCAA70300.038.0701451
ATCAACG88550.030.0048523
TATCAAC88950.029.8208272
TCAACGC89500.029.6874474
CAACGCA91400.029.0692525
AACGCAG93900.028.3438666
GTACATG67050.025.6649971
ACGCAGA104550.025.274787
CGCAGAG104850.025.020248
TACATGG69900.023.8704932
TATACCG1000.001647734523.7650225
GCAGAGT109300.023.1754369
ACATGGG70900.022.8600083
GAGTACT54300.022.05212612-13
GTCTACG1100.002864689821.6077121
AGTACTT56900.020.96096212-13
GTACTTT56500.020.94039514-15
CATGGGA42100.020.3223744
AGAGTAC98600.019.85392610-11
Produced by FastQC (version 0.11.5)