##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936863_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1377540 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76842196959798 33.0 31.0 34.0 30.0 34.0 2 31.856818676771635 33.0 31.0 34.0 30.0 34.0 3 31.868496740566517 33.0 31.0 34.0 30.0 34.0 4 35.442773349594205 37.0 35.0 37.0 33.0 37.0 5 35.47274707086546 37.0 35.0 37.0 33.0 37.0 6 35.53512783657825 37.0 35.0 37.0 33.0 37.0 7 35.47026801399596 37.0 35.0 37.0 33.0 37.0 8 35.439124090770505 37.0 35.0 37.0 33.0 37.0 9 37.05881498903843 39.0 37.0 39.0 33.0 39.0 10-11 36.93346835663574 39.0 37.0 39.0 33.0 39.0 12-13 36.82837558256021 39.0 37.0 39.0 33.0 39.0 14-15 37.97372236014925 40.0 38.0 41.0 33.0 41.0 16-17 37.90852715710614 40.0 38.0 41.0 33.0 41.0 18-19 37.87667762823584 40.0 38.0 41.0 33.0 41.0 20-21 37.83155588948415 40.0 38.0 41.0 32.5 41.0 22-23 37.6970000871118 40.0 37.0 41.0 32.0 41.0 24-25 37.5265658347489 40.0 37.0 41.0 32.0 41.0 26-27 37.48426978526939 40.0 37.0 41.0 32.0 41.0 28-29 37.54866138188365 40.0 37.0 41.0 32.0 41.0 30-31 37.41226534256718 40.0 37.0 41.0 32.0 41.0 32-33 37.223119473844676 40.0 36.5 41.0 31.0 41.0 34-35 37.03815134224777 39.5 36.0 41.0 31.0 41.0 36-37 36.8942825616679 39.0 36.0 41.0 30.5 41.0 38-39 36.72978606791817 39.0 36.0 41.0 30.0 41.0 40-41 36.51201271832397 39.0 35.0 41.0 30.0 41.0 42-43 36.29848062488204 39.0 35.0 41.0 30.0 41.0 44-45 36.041493894914126 39.0 35.0 40.5 29.5 41.0 46-47 35.67581050278032 39.0 35.0 40.0 27.5 41.0 48-49 35.332084730751916 39.0 35.0 40.0 26.5 41.0 50-51 34.673954658303934 38.0 34.0 39.5 25.5 40.5 52-53 34.977881223049785 38.5 34.5 40.0 26.5 40.5 54-55 35.2837946629499 39.0 35.0 40.5 26.5 41.0 56-57 35.178765044935176 39.0 35.0 41.0 26.0 41.0 58-59 34.942704386079534 38.5 34.5 40.0 26.0 41.0 60-61 34.66432045530438 38.0 34.0 40.0 26.0 41.0 62-63 34.368157730447024 38.0 34.0 40.0 25.0 41.0 64-65 34.05598712197105 37.0 34.0 40.0 24.0 41.0 66-67 33.6869426659117 37.0 33.5 39.5 23.0 41.0 68-69 33.254260856309074 36.0 33.0 39.0 22.0 41.0 70-71 32.90590073609478 36.0 33.0 39.0 22.0 40.5 72-73 32.525406884736555 35.0 32.0 38.5 20.5 40.0 74-75 32.096163813754956 35.0 32.0 37.5 19.5 39.5 76-77 31.657173294423394 35.0 31.5 37.0 18.0 39.0 78-79 31.232776906659698 35.0 31.0 36.5 17.0 39.0 80-81 30.815634028775932 35.0 31.0 36.0 12.5 38.0 82-83 30.417182804129098 34.5 31.0 35.5 7.5 37.0 84-85 30.07138050437737 34.0 30.0 35.0 7.0 37.0 86-87 29.738568752994468 34.0 30.0 35.0 2.0 36.0 88-89 29.381552985757217 34.0 30.0 35.0 2.0 36.0 90-91 29.055798379720372 34.0 29.0 35.0 2.0 36.0 92-93 28.71662238483093 34.0 29.0 35.0 2.0 35.0 94-95 28.330843024521975 34.0 29.0 35.0 2.0 35.0 96-97 27.90518315257633 34.0 29.0 35.0 2.0 35.0 98-99 27.333157657853853 34.0 27.0 35.0 2.0 35.0 100-101 25.213965111720896 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.51735997296894 1101 2 0.45672618401250276 1101 3 0.5871731849647226 1101 4 0.32205386220602605 1101 5 0.3114166625490782 1101 6 0.3956958777205344 1101 7 0.38173603499851794 1101 8 0.3439888723082234 1101 9 0.6171817532905948 1101 10-11 0.597901446648379 1101 12-13 0.6708848288767157 1101 14-15 0.8663812206443069 1101 16-17 0.7946374827823988 1101 18-19 0.8971636539486525 1101 20-21 0.8410696088754435 1101 22-23 0.9298284011831583 1101 24-25 0.934489822639577 1101 26-27 1.079992870791564 1101 28-29 0.9788317951668191 1101 30-31 0.6867290524149396 1101 32-33 0.7089228469239472 1101 34-35 0.6141352109823615 1101 36-37 0.7353456516645664 1101 38-39 0.8099714347889417 1101 40-41 0.8500034236174301 1101 42-43 0.8497854755890017 1101 44-45 0.8569243750892426 1101 46-47 0.9391144342376094 1101 48-49 0.9624225137664268 1101 50-51 0.8046521458650133 1101 52-53 0.7833500980157027 1101 54-55 0.8341504637347015 1101 56-57 0.5541817696593938 1101 58-59 0.5853642617639068 1101 60-61 0.6279344296033571 1101 62-63 0.7272093341567114 1101 64-65 0.6894585688674013 1101 66-67 0.7624258139680293 1101 68-69 0.755942204070287 1101 70-71 0.5712799871779168 1101 72-73 0.6280949306977561 1101 74-75 0.5457129943035355 1101 76-77 0.643102568592365 1101 78-79 0.6838209136025455 1101 80-81 0.699326370321657 1101 82-83 0.5473213814903595 1101 84-85 0.73086787424986 1101 86-87 1.060809232146294 1101 88-89 0.9866966731560147 1101 90-91 0.9294118549179942 1101 92-93 0.8477138628297141 1101 94-95 0.8508273352081055 1101 96-97 0.8839842932493767 1101 98-99 0.752140071214118 1101 100-101 1.1907336117957925 1102 1 0.3734847707220865 1102 2 0.5409467577321152 1102 3 0.34291394266536557 1102 4 0.38963961995754914 1102 5 0.3782929536217239 1102 6 0.3301131523997398 1102 7 0.36324512720273106 1102 8 0.34238392273002205 1102 9 0.46941323562006687 1102 10-11 0.5645172336048603 1102 12-13 0.45722810410541825 1102 14-15 0.6255911605044915 1102 16-17 0.611713527597253 1102 18-19 0.6307872051489056 1102 20-21 0.5821865202735168 1102 22-23 0.6870342910184064 1102 24-25 0.5697828388905535 1102 26-27 0.6466503791103975 1102 28-29 0.7639673895944696 1102 30-31 0.750530173803746 1102 32-33 0.8392430838198806 1102 34-35 0.8322909232474203 1102 36-37 0.6742289437853302 1102 38-39 0.6175221872998478 1102 40-41 0.6692131599587725 1102 42-43 0.7541131002188521 1102 44-45 0.9557775464215794 1102 46-47 0.7898818020266773 1102 48-49 0.8750526895049546 1102 50-51 0.5203159734438572 1102 52-53 0.7113772881328231 1102 54-55 0.726427534284511 1102 56-57 0.708174300517932 1102 58-59 0.6285192108333248 1102 60-61 0.709259133737838 1102 62-63 0.7274425667482234 1102 64-65 0.7851979019361579 1102 66-67 0.6230984437367297 1102 68-69 0.6464985228214957 1102 70-71 0.8611388468618486 1102 72-73 0.9561109272786439 1102 74-75 0.9856102172889507 1102 76-77 1.0457916630352848 1102 78-79 1.1211914501799676 1102 80-81 1.0093403113622479 1102 82-83 0.9023140039323785 1102 84-85 0.8809176346064262 1102 86-87 0.9886811373879176 1102 88-89 0.9211782597888956 1102 90-91 0.8554024319956675 1102 92-93 0.8564063560372759 1102 94-95 0.9013034675360565 1102 96-97 0.7773799033479776 1102 98-99 0.8928129045016604 1102 100-101 1.4363853300036098 1103 1 0.36089011274047067 1103 2 0.5158050473742861 1103 3 0.45188652072089397 1103 4 0.3254315596591013 1103 5 0.31803690240999316 1103 6 0.3046084493006518 1103 7 0.3570478443670453 1103 8 0.29916100836692294 1103 9 0.3668164614205267 1103 10-11 0.4816808767577072 1103 12-13 0.30211546663958444 1103 14-15 0.5129427230655139 1103 16-17 0.6534762887818673 1103 18-19 0.6313097510520578 1103 20-21 0.5131143200502919 1103 22-23 0.4464283088533989 1103 24-25 0.7696077395231526 1103 26-27 0.7681049237534836 1103 28-29 0.7051746528709515 1103 30-31 0.7189432967351834 1103 32-33 0.7837079837979033 1103 34-35 0.682646049906225 1103 36-37 0.6681413769933684 1103 38-39 0.623693993698268 1103 40-41 0.7440672097267154 1103 42-43 0.8375342993668582 1103 44-45 0.8237848687587572 1103 46-47 0.7095968080945383 1103 48-49 0.7034357837130969 1103 50-51 0.6894707768308521 1103 52-53 0.768834092554485 1103 54-55 0.6990675142901495 1103 56-57 0.8161183371534264 1103 58-59 0.7411758304857372 1103 60-61 0.761561515617359 1103 62-63 0.7678067273144933 1103 64-65 0.82951593118311 1103 66-67 0.8464914663170333 1103 68-69 0.7052142723550148 1103 70-71 0.8482778950249639 1103 72-73 0.7659888300583262 1103 74-75 0.7663374970488661 1103 76-77 0.6149728354646449 1103 78-79 0.6578198724372264 1103 80-81 0.7037662528467763 1103 82-83 0.5736051088621998 1103 84-85 0.8028866545148681 1103 86-87 0.7842924212553122 1103 88-89 0.7155886178760262 1103 90-91 0.6010575836846073 1103 92-93 0.7887837093069408 1103 94-95 0.7074791046980309 1103 96-97 0.7543250293336108 1103 98-99 0.8088712050038609 1103 100-101 1.212806905905353 1104 1 0.3251146128547262 1104 2 0.45419184639619914 1104 3 0.3883448484314691 1104 4 0.378882494116624 1104 5 0.44542936377489184 1104 6 0.2885408538442036 1104 7 0.30614109579702387 1104 8 0.4468313869540168 1104 9 0.34135445057804503 1104 10-11 0.4555837190847498 1104 12-13 0.46690314227208063 1104 14-15 0.5566613368260604 1104 16-17 0.6187127055461517 1104 18-19 0.49759982782745027 1104 20-21 0.5429797157035807 1104 22-23 0.5965314977223244 1104 24-25 0.5095717898526928 1104 26-27 0.6043516558060844 1104 28-29 0.6834583889737402 1104 30-31 0.7669141555508574 1104 32-33 0.8142544079932108 1104 34-35 0.7839643844180912 1104 36-37 0.7474955064373674 1104 38-39 0.6523905752408652 1104 40-41 0.7776349896096448 1104 42-43 0.8500582073371419 1104 44-45 0.7497614744837193 1104 46-47 0.8836711220729896 1104 48-49 0.8475374647830236 1104 50-51 0.7142814472976013 1104 52-53 0.7894486555896663 1104 54-55 0.6427695746488951 1104 56-57 0.7352382359617593 1104 58-59 0.7799460566735732 1104 60-61 0.751972237903459 1104 62-63 0.7680334369480306 1104 64-65 0.6606789695959989 1104 66-67 0.5032115177045569 1104 68-69 0.7329313243380966 1104 70-71 0.6884443646701612 1104 72-73 0.6846193239434655 1104 74-75 0.6280750284688423 1104 76-77 0.46236018686637337 1104 78-79 0.595543455816891 1104 80-81 0.6961874937401582 1104 82-83 0.6338639896723102 1104 84-85 0.6827026598551384 1104 86-87 0.7094874185293953 1104 88-89 0.711858884547734 1104 90-91 0.5842614586200554 1104 92-93 0.5310821491488227 1104 94-95 0.4907141072260224 1104 96-97 0.44801422482029096 1104 98-99 0.6745533041191543 1104 100-101 0.8506420448189829 1105 1 0.1921582522387908 1105 2 0.3301486542679015 1105 3 0.2917695497285102 1105 4 0.2685604699648181 1105 5 0.2802865613980501 1105 6 0.2935724812162519 1105 7 0.25029162930925963 1105 8 0.30477017680770047 1105 9 0.40745885285266326 1105 10-11 0.32482370375026903 1105 12-13 0.34251971629290523 1105 14-15 0.3360508327049203 1105 16-17 0.49468069481201127 1105 18-19 0.4671433074773148 1105 20-21 0.3626717481615742 1105 22-23 0.40539674594927533 1105 24-25 0.41234956763047137 1105 26-27 0.442814054374864 1105 28-29 0.5462834480753926 1105 30-31 0.3550922272395738 1105 32-33 0.1772104491407518 1105 34-35 -0.044302180018689796 1105 36-37 0.1216185656668145 1105 38-39 0.2694629667813402 1105 40-41 0.37608876253150925 1105 42-43 0.17026138924245515 1105 44-45 0.16232297215159974 1105 46-47 0.10725108501465286 1105 48-49 -0.024456337529926486 1105 50-51 -0.05700153781517514 1105 52-53 -0.03272245168783172 1105 54-55 -0.06756522823910416 1105 56-57 0.09475360239201791 1105 58-59 0.07676894339007845 1105 60-61 0.014918694998854676 1105 62-63 -0.07774319074349023 1105 64-65 0.09046620363854885 1105 66-67 0.19924052543569104 1105 68-69 0.2879677437362176 1105 70-71 0.14996599268114608 1105 72-73 0.1741663177457724 1105 74-75 0.2347567101505348 1105 76-77 -0.027565376995358548 1105 78-79 0.0871749809590554 1105 80-81 0.0968999082823565 1105 82-83 0.31531405676067337 1105 84-85 0.34247679569310563 1105 86-87 0.30839962861182 1105 88-89 0.5568016997033141 1105 90-91 0.2919127269096222 1105 92-93 0.30824015822172157 1105 94-95 0.21280055537630105 1105 96-97 0.3138215214786513 1105 98-99 0.44032865393705833 1105 100-101 0.7177719406722822 1106 1 0.5472352020503521 1106 2 0.5099262944736793 1106 3 0.4635118990962219 1106 4 0.2541217456192797 1106 5 0.3173413983209272 1106 6 0.402392837527735 1106 7 0.5018058263775274 1106 8 0.4695741910138196 1106 9 0.46154999068170355 1106 10-11 0.5616977964086303 1106 12-13 0.5036558980906847 1106 14-15 0.725100373105839 1106 16-17 0.6444916238212386 1106 18-19 0.8047191983465183 1106 20-21 0.8383432262278916 1106 22-23 0.9202255593183679 1106 24-25 0.7756514223182407 1106 26-27 0.8635956624467269 1106 28-29 0.8042938492425833 1106 30-31 0.6421529471755676 1106 32-33 0.8620207807978701 1106 34-35 0.7121818055145255 1106 36-37 0.8938762669835185 1106 38-39 0.8535122175569967 1106 40-41 0.7132858778639033 1106 42-43 0.8202845592633423 1106 44-45 0.89693391491803 1106 46-47 0.6353519759743591 1106 48-49 0.7972978683719134 1106 50-51 0.7054442027023171 1106 52-53 0.849009978014692 1106 54-55 0.7841185806227102 1106 56-57 0.9256074719665222 1106 58-59 1.0471733145593376 1106 60-61 1.0220493594353997 1106 62-63 0.9019993323993774 1106 64-65 0.8328246221398174 1106 66-67 0.9149860027562227 1106 68-69 0.7206330383032125 1106 70-71 0.879367753602736 1106 72-73 0.7598972051937807 1106 74-75 0.8492921874348554 1106 76-77 1.0906003719212656 1106 78-79 1.0826753810039484 1106 80-81 1.0672685288334414 1106 82-83 1.1568107496022257 1106 84-85 1.2220854576606008 1106 86-87 1.3309483128080544 1106 88-89 1.1423244189948925 1106 90-91 1.3372761885107423 1106 92-93 1.4035359199180597 1106 94-95 1.5075019517320918 1106 96-97 1.4390075152010233 1106 98-99 1.6976407867012782 1106 100-101 1.875300930350761 1107 1 0.16714010291171277 1107 2 0.2423524479426291 1107 3 0.29480839859580854 1107 4 0.2615194617929646 1107 5 0.1203211847297112 1107 6 0.04392616041061359 1107 7 0.23858298650662846 1107 8 0.18504046217096715 1107 9 0.3249585270955109 1107 10-11 0.34560701009229433 1107 12-13 0.34310375233095414 1107 14-15 0.24321935153368912 1107 16-17 0.331823059995088 1107 18-19 0.2962673930304476 1107 20-21 0.36510794629638355 1107 22-23 0.4850048135578362 1107 24-25 0.41180276098130264 1107 26-27 0.4868331530513643 1107 28-29 0.40631490690899597 1107 30-31 0.5739079603793087 1107 32-33 0.6717772852011379 1107 34-35 0.7303460022563968 1107 36-37 0.5313419513074322 1107 38-39 0.5452117428651846 1107 40-41 0.6166231904088733 1107 42-43 0.5765573442693466 1107 44-45 0.7780831031990445 1107 46-47 0.772160989893564 1107 48-49 0.8142868913749695 1107 50-51 0.6051394312658189 1107 52-53 0.7575760349375145 1107 54-55 0.8112344962634239 1107 56-57 0.7947964161892287 1107 58-59 0.7861400459384313 1107 60-61 0.7836101750532052 1107 62-63 0.7866278187313895 1107 64-65 0.814266021214543 1107 66-67 0.8703002599901453 1107 68-69 0.7926633004086554 1107 70-71 0.8435518343445594 1107 72-73 0.8889985062753993 1107 74-75 0.9276700959773052 1107 76-77 0.8161689168562098 1107 78-79 0.8759819651924126 1107 80-81 0.8928735498750306 1107 82-83 0.915386654068989 1107 84-85 0.8173353240153638 1107 86-87 0.8598669319010348 1107 88-89 0.8632344727343444 1107 90-91 0.8827989725119032 1107 92-93 1.0357326658072097 1107 94-95 1.0313817433661008 1107 96-97 0.9701081691583298 1107 98-99 0.7797210703105435 1107 100-101 0.9055313829760365 1108 1 -0.011954344647669757 1108 2 0.0923309142566282 1108 3 -0.023505391901743167 1108 4 0.08174324960896229 1108 5 0.10563074162332953 1108 6 0.07416920665522042 1108 7 0.06339060536851093 1108 8 -0.0025350073040115717 1108 9 -0.19189706719359378 1108 10-11 -0.05831841834680063 1108 12-13 -0.20437680640724665 1108 14-15 0.1972678876493319 1108 16-17 0.13898808928902184 1108 18-19 0.15315817254987962 1108 20-21 0.20416659918149094 1108 22-23 0.2459295025382815 1108 24-25 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-1.2857299237368878 2316 68-69 -0.8295086156018456 2316 70-71 -1.3967293805035865 2316 72-73 -1.3518113924872566 2316 74-75 -1.5029813850875655 2316 76-77 -1.7875121855059994 2316 78-79 -2.029621709348273 2316 80-81 -1.7258609833083192 2316 82-83 -1.6787968854885627 2316 84-85 -1.3751684255159482 2316 86-87 -1.2184823369403581 2316 88-89 -1.704052741363892 2316 90-91 -1.7454125010539023 2316 92-93 -2.1836561605894893 2316 94-95 -2.118019269007352 2316 96-97 -1.9863025919965267 2316 98-99 -2.0841596945938434 2316 100-101 -1.5917839526070168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3643.0 3 960.0 4 1052.0 5 1137.0 6 1325.0 7 1449.0 8 1710.0 9 2150.0 10 2822.0 11 3606.0 12 4616.0 13 5686.0 14 6451.0 15 8097.0 16 10240.0 17 13435.0 18 11826.0 19 7848.0 20 7419.0 21 7701.0 22 8122.0 23 9173.0 24 10533.0 25 12075.0 26 14302.0 27 16613.0 28 20225.0 29 24707.0 30 30074.0 31 37542.0 32 48118.0 33 63139.0 34 85914.0 35 124871.0 36 186911.0 37 278008.0 38 256734.0 39 47275.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.250106232680444 18.973701509775225 14.436530967280333 22.339661290264 2 15.185312759336853 20.602046644662547 40.69417617538184 23.51846442061876 3 19.165721682998356 25.51455904434728 29.915289266704782 25.404430005949585 4 12.645543264589222 16.96538658384804 37.68392830140396 32.705141850158775 5 13.629446162030638 37.69617184195494 33.86183630071179 14.812545695302632 6 30.811833325339418 36.491512638385636 18.10121173274731 14.595442303527644 7 27.834294772325386 33.47246761911753 20.96012105627707 17.733116552280016 8 26.208094829421714 34.62295610898233 20.059907870937923 19.109041190658036 9 26.700304983529726 17.054219617215143 20.6057949778022 35.63968042145293 10-11 24.486929535597415 27.16614307284465 27.485604955274972 20.86132243628296 12-13 25.84422185960441 24.594062442205516 28.000816798433547 21.560898899756523 14-15 22.792864319234326 25.671445467709024 26.734276012550485 24.801414200506162 16-17 21.785962448685297 29.170155485003896 27.003235879007782 22.04064618730303 18-19 21.633966814624703 27.500429631766522 29.768304039047262 21.09729951456152 20-21 22.605944648214617 26.50418896342635 29.841555723221457 21.048310665137574 22-23 22.210573311667815 26.630499342296698 29.6723180835532 21.486609262482286 24-25 21.992243672330932 26.7944907335603 29.768439802739234 21.44482579136953 26-27 21.810739793092555 26.969624882645306 29.6247010519702 21.594934272291944 28-29 21.538973990701702 26.996484169682493 29.856442680051448 21.608099159564357 30-31 21.889559661541266 26.823751671030138 29.71686275962479 21.569825907803803 32-33 21.601168247124505 26.97543913678711 29.661448752817748 21.761943863270634 34-35 22.02770181688765 26.93551374377635 29.509335018394033 21.527449420941963 36-37 22.01973899723047 26.75692401110718 29.465261732740544 21.758075258921806 38-39 21.83720445431322 26.974329808321585 29.523771130640437 21.664694606724762 40-41 22.02346271146299 26.888546308633167 29.485892296448483 21.60209868345536 42-43 21.882178334343113 27.04313284486153 29.205405154089465 21.869283666705897 44-45 21.99436856653397 27.217553976378195 28.979692192369416 21.80838526471841 46-47 22.1164844630009 27.314463458439338 28.569675199845456 21.99937687871431 48-49 22.226697079556388 27.480474147651652 28.289325794752212 22.003502978039748 50-51 22.160704145371948 27.67587295639684 28.187890221639357 21.975532676591857 52-53 22.353375245488248 27.687951030179075 28.029056221166027 21.929617503166654 54-55 22.06517306135582 27.628913977209873 28.07887677084328 22.227036190591022 56-57 22.10937287196855 27.980351743737348 27.883381606544578 22.026893777749528 58-59 22.336765640606558 27.539235449014797 27.98176700263325 22.14223190774539 60-61 22.211415185138105 27.53224254090472 28.34194708264478 21.9143951913124 62-63 22.166455452751464 27.611343149786084 28.355368229576992 21.86683316788546 64-65 22.101164291384418 27.601934505976878 28.221519672246636 22.075381530392065 66-67 22.058045250755615 27.828426488498792 27.930204183782475 22.183324076963114 68-69 21.938087655884985 27.770517834520753 28.18750653758092 22.103887972013343 70-71 21.987002674766607 27.74550251083867 28.034073774470734 22.233421039923986 72-73 22.07658585730234 27.690243880301697 27.96580048467466 22.267369777721303 74-75 21.90648609179138 28.05144924299942 27.799424347689055 22.24264031752014 76-77 22.095866791396 27.9168433988356 27.732654584965076 22.25463522480332 78-79 22.030370084094173 27.973407470259026 27.688238439230773 22.30798400641603 80-81 22.00758516607621 28.156261393363923 27.64424089511351 22.191912545446357 82-83 22.05210364649978 28.08426281298091 27.670496211195307 22.193137329324 84-85 22.082266655430832 27.87835949055173 27.562097362387654 22.477276491629787 86-87 22.17134085499343 27.754504405514368 27.728714611226117 22.345440128266087 88-89 22.187533153029296 27.699256064188628 27.627136871691054 22.486073911091022 90-91 22.026865982818933 27.750062019935733 27.85790224786399 22.365169749381344 92-93 22.069316264310714 27.72472568290924 27.756766427693087 22.449191625086957 94-95 21.944377736416783 27.67191082591966 27.758269968780642 22.625441468882915 96-97 21.921579527762184 27.765163695423507 27.664008807451452 22.64924796936286 98-99 21.774068389295824 27.807425989618867 27.555512101934443 22.86299351915087 100-101 21.8013337074421 27.822134542499388 27.69714490075699 22.679386849301526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 383.0 1 293.5 2 231.5 3 284.0 4 292.0 5 314.5 6 492.0 7 907.0 8 1488.5 9 1800.5 10 1645.5 11 1532.0 12 1644.5 13 1764.0 14 1886.5 15 2087.0 16 2206.0 17 2254.5 18 2336.5 19 2526.5 20 2779.0 21 3123.0 22 3709.5 23 4563.0 24 5523.5 25 6764.0 26 8666.0 27 10749.5 28 12541.0 29 14560.0 30 17149.5 31 19759.0 32 22528.5 33 25435.0 34 28873.5 35 31393.0 36 33936.0 37 37667.0 38 39719.5 39 40650.0 40 41709.0 41 43887.5 42 46194.0 43 48032.5 44 50045.5 45 51664.5 46 52149.5 47 52236.0 48 54784.0 49 54652.0 50 51330.5 51 48914.0 52 46188.5 53 44621.0 54 43057.0 55 39542.5 56 35366.5 57 31382.0 58 28145.0 59 25154.0 60 21470.0 61 17422.0 62 13746.0 63 10478.0 64 7864.5 65 6033.5 66 4403.0 67 3320.5 68 2375.5 69 1517.5 70 1043.0 71 716.5 72 501.5 73 389.0 74 303.5 75 222.0 76 152.0 77 85.0 78 47.0 79 26.5 80 19.0 81 18.5 82 12.5 83 7.5 84 5.0 85 5.0 86 6.0 87 7.0 88 4.5 89 4.0 90 4.0 91 3.0 92 2.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061341231470592505 2 0.10409861056666231 3 0.07063315765785386 4 0.1454767193693105 5 0.11455202752733135 6 0.10845420096694107 7 0.11985132918100382 8 0.10359045835329646 9 0.12544100352802823 10-11 0.1185083554742512 12-13 0.10420750032666928 14-15 0.10870827707362399 16-17 0.12456988544797248 18-19 0.09883560549965881 20-21 0.0817036165918957 22-23 0.084171784485387 24-25 0.07161316549791658 26-27 0.06079678267055766 28-29 0.07633172176488523 30-31 0.05586044688357507 32-33 0.056368599096940926 34-35 0.053355982403414784 36-37 0.056114522990257994 38-39 0.05858269088374929 40-41 0.0768035773915821 42-43 0.07273835968465525 44-45 0.05832861477706637 46-47 0.043628497176125554 48-49 0.051650042829972265 50-51 0.03179580992203493 52-53 0.050234475949881674 54-55 0.055134515150195275 56-57 0.060070850937177865 58-59 0.06642275360425107 60-61 0.06279309493735209 62-63 0.07066945424452285 64-65 0.04725815584302452 66-67 0.07335540165802808 68-69 0.0641723652307737 70-71 0.029799497655240502 72-73 0.02377426426818822 74-75 0.035824731042292786 76-77 0.050234475949881674 78-79 0.07172205525792355 80-81 0.04660481728298271 82-83 0.06667682971093399 84-85 0.07201242795127545 86-87 0.07451689243143575 88-89 0.09771041131292012 90-91 0.07016130203115699 92-93 0.08475252987209082 94-95 0.09513335365942187 96-97 0.10398972080665535 98-99 0.13585812390202825 100-101 0.1325551345151502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1377540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.34193618415942 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49787887289392 53.90571136641148 2 10.758135703329534 14.05914833174971 3 2.963834002293882 5.809879567149859 4 1.2465276512812928 3.2580212096724943 5 0.66150019775597 2.161185185378972 6 0.3962388580327151 1.5534608505154723 7 0.25988514051542894 1.1886978786739322 8 0.19645682744277967 1.0269495585366823 9 0.14003710402315747 0.8235265963046086 >10 0.7971856250962318 9.569281913378914 >50 0.057176957309831605 2.6089124079057395 >100 0.024364309429967295 2.789922043805939 >500 3.337502551452231E-4 0.13222849347084248 >1k 3.337502551452231E-4 0.6431146133790783 >5k 1.1125008504840768E-4 0.46995998366629826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6465 0.46931486563003616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4649 0.33748566284826576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3009 0.2184328585739797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.6296586668989646E-5 0.0 0.0 0.0 0.0 12-13 7.259317333797929E-5 0.0 0.0 0.0 0.0 14-15 1.4518634667595859E-4 0.0 0.0 0.0 0.0 16-17 2.9037269335191717E-4 0.0 0.0 7.259317333797929E-5 0.0 18-19 2.9037269335191717E-4 0.0 0.0 7.259317333797929E-5 0.0 20-21 2.9037269335191717E-4 0.0 0.0 7.259317333797929E-5 0.0 22-23 2.9037269335191717E-4 0.0 0.0 2.9037269335191717E-4 0.0 24-25 2.9037269335191717E-4 0.0 0.0 5.807453867038343E-4 0.0 26-27 2.9037269335191717E-4 0.0 0.0 0.0011977873600766582 0.0 28-29 3.629658666898965E-4 0.0 0.0 0.001851125920118472 0.0 30-31 3.629658666898965E-4 0.0 0.0 0.0033392859735470477 0.0 32-33 3.629658666898965E-4 0.0 0.0 0.005988936800383292 0.0 34-35 3.629658666898965E-4 0.0 0.0 0.008094138827184693 0.0 36-37 3.629658666898965E-4 0.0 0.0 0.011469721387400728 0.0 38-39 3.629658666898965E-4 0.0 0.0 0.016660133281066247 0.0 40-41 3.629658666898965E-4 0.0 0.0 0.021741655414724798 0.0 42-43 3.9926245335888613E-4 3.6296586668989646E-5 0.0 0.02907356592186071 0.0 44-45 4.355590400278758E-4 7.259317333797929E-5 0.0 0.035933620802299754 0.0 46-47 4.718556266968654E-4 7.259317333797929E-5 0.0 0.049327061283156934 0.0 48-49 5.08152213365855E-4 7.259317333797929E-5 0.0 0.06526126283084338 0.0 50-51 5.08152213365855E-4 7.259317333797929E-5 0.0 0.07310132555134516 0.0 52-53 5.08152213365855E-4 7.259317333797929E-5 0.0 0.08457104693874588 0.0 54-55 5.08152213365855E-4 7.259317333797929E-5 0.0 0.10464305936669716 0.0 56-57 5.807453867038343E-4 7.259317333797929E-5 0.0 0.13654775904873906 0.0 58-59 5.807453867038343E-4 7.259317333797929E-5 0.0 0.1791599517981329 0.0 60-61 5.807453867038343E-4 7.259317333797929E-5 0.0 0.2289951652946557 0.0 62-63 5.807453867038343E-4 7.259317333797929E-5 0.0 0.2833311555381332 0.0 64-65 5.807453867038343E-4 7.259317333797929E-5 0.0 0.34626943682216127 0.0 66-67 5.807453867038343E-4 7.259317333797929E-5 0.0 0.4292797305341406 0.0 68-69 5.807453867038343E-4 7.259317333797929E-5 0.0 0.53276129912743 0.0 70-71 5.807453867038343E-4 7.259317333797929E-5 0.0 0.5868795098508937 0.0 72-73 6.533385600418136E-4 7.259317333797929E-5 0.0 0.6534111532151516 7.259317333797929E-5 74-75 6.533385600418136E-4 7.259317333797929E-5 0.0 0.7319206701801763 7.259317333797929E-5 76-77 6.533385600418136E-4 7.259317333797929E-5 0.0 0.8168183863989431 7.259317333797929E-5 78-79 6.533385600418136E-4 7.259317333797929E-5 0.0 0.9027687036311105 7.259317333797929E-5 80-81 6.533385600418136E-4 7.259317333797929E-5 0.0 1.044652060920191 7.259317333797929E-5 82-83 6.533385600418136E-4 7.259317333797929E-5 0.0 1.1989125542633972 7.259317333797929E-5 84-85 7.622283200487826E-4 7.259317333797929E-5 0.0 1.4421359815322967 7.259317333797929E-5 86-87 7.985249067177723E-4 7.259317333797929E-5 0.0 1.7855379880076079 7.259317333797929E-5 88-89 7.985249067177723E-4 7.259317333797929E-5 0.0 2.143603815497191 7.259317333797929E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4130 0.0 39.249645 1 GTATCAA 7030 0.0 38.070145 1 ATCAACG 8855 0.0 30.004852 3 TATCAAC 8895 0.0 29.820827 2 TCAACGC 8950 0.0 29.687447 4 CAACGCA 9140 0.0 29.069252 5 AACGCAG 9390 0.0 28.343866 6 GTACATG 6705 0.0 25.664997 1 ACGCAGA 10455 0.0 25.27478 7 CGCAGAG 10485 0.0 25.02024 8 TACATGG 6990 0.0 23.870493 2 TATACCG 100 0.0016477345 23.765022 5 GCAGAGT 10930 0.0 23.175436 9 ACATGGG 7090 0.0 22.860008 3 GAGTACT 5430 0.0 22.052126 12-13 GTCTACG 110 0.0028646898 21.607712 1 AGTACTT 5690 0.0 20.960962 12-13 GTACTTT 5650 0.0 20.940395 14-15 CATGGGA 4210 0.0 20.322374 4 AGAGTAC 9860 0.0 19.853926 10-11 >>END_MODULE