FastQCFastQC Report
Thu 26 May 2016
SRR936863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377540
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77190.5603467049958623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61170.4440524413084194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.29581718135226565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.11455202752733135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.039.934761
GTATCAA80100.038.6725771
TATCAAC101000.030.5233752
ATCAACG101000.030.4282073
TCAACGC101700.030.2621784
CAACGCA103000.029.9724525
AACGCAG106350.029.0729876
GTACGCT908.9484715E-426.388883
ACGCAGA119900.025.70827
CGCAGAG121400.025.2731868
GCAGAGT126500.023.8788139
GTACATG66800.023.1150551
TACATGG67600.022.6968212
GAGTACT71300.022.6149912-13
ACATGGG67050.022.1737423
AGTACTT74050.022.0958312-13
GTGTACG1754.0425784E-621.719011
TGTACGC1100.002877052621.5916862
GTACTTT75800.021.08596614-15
AGAGTAC114100.020.47979510-11
Produced by FastQC (version 0.11.5)