##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1377540 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.150937177867796 33.0 31.0 34.0 31.0 34.0 2 32.144882907211404 33.0 31.0 34.0 30.0 34.0 3 32.32128939994483 33.0 31.0 34.0 31.0 34.0 4 36.057087271512984 37.0 35.0 37.0 35.0 37.0 5 35.990391567576985 37.0 35.0 37.0 35.0 37.0 6 36.03716189729518 37.0 35.0 37.0 35.0 37.0 7 35.97599634130406 37.0 35.0 37.0 35.0 37.0 8 35.961633781959144 37.0 35.0 37.0 35.0 37.0 9 37.64818807439348 39.0 37.0 39.0 35.0 39.0 10-11 37.579336716175206 39.0 37.0 39.0 35.0 39.0 12-13 37.4838491078299 39.0 37.0 39.0 35.0 39.0 14-15 38.68771578320775 40.0 38.0 41.0 34.5 41.0 16-17 38.60674898732523 40.0 38.0 41.0 34.0 41.0 18-19 38.68092723260305 40.0 38.0 41.0 34.5 41.0 20-21 38.77023643596556 40.0 38.0 41.0 35.0 41.0 22-23 38.699761168459716 40.0 38.0 41.0 34.0 41.0 24-25 38.604687341202435 40.0 38.0 41.0 34.0 41.0 26-27 38.44920583068368 40.0 38.0 41.0 34.0 41.0 28-29 38.27272529291345 40.0 38.0 41.0 34.0 41.0 30-31 38.115680488406866 40.0 37.5 41.0 34.0 41.0 32-33 38.085235637440654 40.0 37.0 41.0 33.5 41.0 34-35 37.99229859024057 40.0 37.0 41.0 33.0 41.0 36-37 37.82414594131568 40.0 37.0 41.0 33.0 41.0 38-39 37.66273247963761 40.0 37.0 41.0 33.0 41.0 40-41 37.46792506932648 40.0 37.0 41.0 33.0 41.0 42-43 37.23199253742178 40.0 36.5 41.0 32.0 41.0 44-45 36.8553098276638 40.0 36.0 41.0 31.0 41.0 46-47 36.53495470185984 39.5 36.0 41.0 30.5 41.0 48-49 36.166998054502955 39.0 36.0 41.0 30.0 41.0 50-51 35.895865818778404 39.0 35.0 41.0 29.0 41.0 52-53 35.641835445794676 39.0 35.0 40.0 28.0 41.0 54-55 35.40765277233329 39.0 35.0 40.0 28.0 41.0 56-57 35.14319983448757 38.0 35.0 40.0 27.0 41.0 58-59 34.82332273473003 38.0 34.0 40.0 26.0 41.0 60-61 34.50358828055809 38.0 34.0 40.0 26.0 41.0 62-63 34.138801777080886 37.0 34.0 40.0 25.5 41.0 64-65 33.74399835939428 37.0 33.0 39.5 24.5 41.0 66-67 33.377189410107874 36.0 33.0 39.0 23.5 40.0 68-69 32.948404765015894 36.0 32.0 39.0 22.5 40.0 70-71 32.47505444488 35.0 32.0 38.0 22.0 40.0 72-73 32.13751215935653 35.0 31.5 37.5 20.5 39.5 74-75 31.707125745894857 35.0 31.0 37.0 20.0 39.0 76-77 30.305505466265952 33.5 29.5 35.5 18.5 38.0 78-79 31.021484675581107 34.0 31.0 36.0 18.5 38.5 80-81 30.85814495404852 34.0 31.0 36.0 18.0 37.5 82-83 30.52730592215108 34.0 31.0 35.0 16.0 37.0 84-85 30.14973793864425 34.0 30.5 35.0 11.0 36.5 86-87 29.781614326988688 34.0 30.0 35.0 7.0 36.0 88-89 29.438053704429635 34.0 30.0 35.0 4.0 36.0 90-91 29.031276405766803 34.0 29.0 35.0 2.0 35.0 92-93 28.587467877520798 34.0 29.0 35.0 2.0 35.0 94-95 28.134619321399015 34.0 29.0 35.0 2.0 35.0 96-97 27.51755956269872 33.0 27.0 35.0 2.0 35.0 98-99 26.81192379168663 33.0 26.5 35.0 2.0 35.0 100-101 23.832008870885783 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3614177649446546 1101 2 0.30686716844613926 1101 3 0.3365074299316717 1101 4 0.2842237555649234 1101 5 0.3302550872411132 1101 6 0.29361606529079864 1101 7 0.353230140100969 1101 8 0.3591887658014201 1101 9 0.36031246694957275 1101 10-11 0.3749813498841874 1101 12-13 0.42778729540828664 1101 14-15 0.4946417992790657 1101 16-17 0.5656527519487256 1101 18-19 0.5635021789339234 1101 20-21 0.5647341351468 1101 22-23 0.5299911098193988 1101 24-25 0.616919882678431 1101 26-27 0.5942438722115995 1101 28-29 0.5186327193087621 1101 30-31 0.4229950516730412 1101 32-33 0.6420109748895797 1101 34-35 0.5942130521056725 1101 36-37 0.5784530440159514 1101 38-39 0.5469598319325044 1101 40-41 0.37727331313159596 1101 42-43 0.318424342049866 1101 44-45 0.4365515051250952 1101 46-47 0.3562709401885087 1101 48-49 0.35509122067652044 1101 50-51 0.44263469219814056 1101 52-53 0.1944681922052709 1101 54-55 0.1535490382415503 1101 56-57 0.2914396022533339 1101 58-59 0.1954117889019713 1101 60-61 0.15095554336753025 1101 62-63 0.37803846971215904 1101 64-65 0.2932925303992775 1101 66-67 0.138985155984777 1101 68-69 0.20123646382775462 1101 70-71 0.2207430398896335 1101 72-73 0.3026827556566971 1101 74-75 0.4244142474142123 1101 76-77 0.28770822053938616 1101 78-79 0.4039347149221584 1101 80-81 0.5632592385797714 1101 82-83 0.5849547907475809 1101 84-85 0.5588149150833921 1101 86-87 0.5767709098825229 1101 88-89 0.5813975219561627 1101 90-91 0.5681349358373637 1101 92-93 0.7772915216397926 1101 94-95 0.7396780212891549 1101 96-97 0.6351525388365467 1101 98-99 0.6971252945234561 1101 100-101 0.7275818089143513 1102 1 0.3245763773532033 1102 2 0.16007228490979486 1102 3 0.2827239129225845 1102 4 0.272683423218659 1102 5 0.2821452684746433 1102 6 0.3298208459483689 1102 7 0.3255344821754349 1102 8 0.30391956125480846 1102 9 0.42601445431105134 1102 10-11 0.40838672888715166 1102 12-13 0.4179491946461127 1102 14-15 0.5624996617206861 1102 16-17 0.597875686486887 1102 18-19 0.5546655943871102 1102 20-21 0.5059190218376912 1102 22-23 0.5180555438843868 1102 24-25 0.5438421690090891 1102 26-27 0.47134698503226957 1102 28-29 0.46020978680578395 1102 30-31 0.37928123986691276 1102 32-33 0.47523888546339776 1102 34-35 0.510141047139804 1102 36-37 0.6781579416968455 1102 38-39 0.6974135771879659 1102 40-41 0.7455233563793513 1102 42-43 0.8959821159607841 1102 44-45 0.9324170198751176 1102 46-47 0.7558002011098424 1102 48-49 0.7822766568682766 1102 50-51 0.8793287321488918 1102 52-53 0.9964282821606005 1102 54-55 0.9459852605248287 1102 56-57 1.008230720691131 1102 58-59 1.0046230516346313 1102 60-61 1.0216598203159677 1102 62-63 1.131391951410734 1102 64-65 1.0379895417251035 1102 66-67 1.050402562951227 1102 68-69 0.808576370819651 1102 70-71 0.8338327611705836 1102 72-73 0.9682296616615176 1102 74-75 0.915761236861762 1102 76-77 0.863593883654648 1102 78-79 0.9127688572428845 1102 80-81 1.0122802111963125 1102 82-83 1.0626118818527885 1102 84-85 1.0610817079317414 1102 86-87 0.9001275461231266 1102 88-89 1.049984185366526 1102 90-91 1.1164505121518395 1102 92-93 1.0544292326633915 1102 94-95 1.1941433264147747 1102 96-97 1.3426473236662524 1102 98-99 1.5865895311751643 1102 100-101 1.984862593683001 1103 1 0.12000728060633747 1103 2 -0.09942029140515274 1103 3 0.042619463248961154 1103 4 0.14649212083714502 1103 5 0.162861241903272 1103 6 0.22131911708386554 1103 7 0.19351223696863684 1103 8 0.27954511936544435 1103 9 0.31562486565695735 1103 10-11 0.3273934156151199 1103 12-13 0.3295228983510583 1103 14-15 0.47852672104433225 1103 16-17 0.5681065625155668 1103 18-19 0.4911851932057445 1103 20-21 0.42935042643735954 1103 22-23 0.5444625219735357 1103 24-25 0.5478083110099092 1103 26-27 0.5384305354731964 1103 28-29 0.6145808181998191 1103 30-31 0.628083010497896 1103 32-33 0.7058811631833422 1103 34-35 0.6851297687024314 1103 36-37 0.7997242479370144 1103 38-39 0.7927333882978544 1103 40-41 0.9766721874943727 1103 42-43 0.9099508279204684 1103 44-45 0.9663553535078506 1103 46-47 1.0377289645282062 1103 48-49 1.1124531434927718 1103 50-51 0.9159861640719384 1103 52-53 1.0164748910236412 1103 54-55 1.0337413591562026 1103 56-57 1.0058422582071387 1103 58-59 1.1255751339893507 1103 60-61 1.085420276347918 1103 62-63 0.9905550448675982 1103 64-65 0.9544863344833914 1103 66-67 0.9996958986499607 1103 68-69 0.9594759122065852 1103 70-71 0.939478711588599 1103 72-73 0.9303614481102116 1103 74-75 0.853257866347704 1103 76-77 1.125678733951755 1103 78-79 1.1966206267767099 1103 80-81 1.348297496494098 1103 82-83 1.2209630022023639 1103 84-85 1.0547243140559779 1103 86-87 0.9722351615695075 1103 88-89 1.3557339755113489 1103 90-91 1.497562393134853 1103 92-93 1.492644781174807 1103 94-95 1.5184111391863944 1103 96-97 1.6025363654137657 1103 98-99 1.568852352052751 1103 100-101 1.6534671432168189 1104 1 0.24193172152418896 1104 2 0.1516739424332414 1104 3 0.11671320948170916 1104 4 0.155968943375683 1104 5 0.11455085842212753 1104 6 0.12098966840211034 1104 7 0.15843665809437368 1104 8 0.22720038397697095 1104 9 0.18996835234316478 1104 10-11 0.2478233722344072 1104 12-13 0.2064807429541986 1104 14-15 0.2617111637254794 1104 16-17 0.2263941490835606 1104 18-19 0.2842996977642258 1104 20-21 0.2886173626365931 1104 22-23 0.2431767544804586 1104 24-25 0.28115657839053654 1104 26-27 0.28066636027343606 1104 28-29 0.32140729716530814 1104 30-31 0.25651545732458914 1104 32-33 0.37642834307744266 1104 34-35 0.4259848238774353 1104 36-37 0.38580301693968266 1104 38-39 0.3876982752938929 1104 40-41 0.6694802741910166 1104 42-43 0.5548611066833544 1104 44-45 0.6057288792204929 1104 46-47 0.7155196971421844 1104 48-49 0.6297291372816503 1104 50-51 0.5120178458435873 1104 52-53 0.49893134388153015 1104 54-55 0.5159770400138157 1104 56-57 0.5904274425526523 1104 58-59 0.37479937320759404 1104 60-61 0.4962634742492327 1104 62-63 0.5420467402778115 1104 64-65 0.49083400630140517 1104 66-67 0.5112393774193222 1104 68-69 0.5239017520668199 1104 70-71 0.5865245591137693 1104 72-73 0.3750442975264363 1104 74-75 0.333963796963765 1104 76-77 0.2884013727079484 1104 78-79 0.3299732124360659 1104 80-81 0.49934548876438356 1104 82-83 0.4021122761017857 1104 84-85 0.6270756348195228 1104 86-87 0.6272971738514137 1104 88-89 0.7361082983062808 1104 90-91 0.8695113942630037 1104 92-93 0.879733307688138 1104 94-95 1.0149982595602154 1104 96-97 1.1624335903798944 1104 98-99 1.0771292328880513 1104 100-101 1.020542966793542 1105 1 0.14732754287745564 1105 2 -0.02992467746324934 1105 3 0.0604356553318155 1105 4 0.07233211505800341 1105 5 0.08707018626060403 1105 6 0.0549651332286345 1105 7 0.12291816087374485 1105 8 -0.008199909933964022 1105 9 0.12664971455644292 1105 10-11 0.07401498938347117 1105 12-13 0.2154418317025204 1105 14-15 0.2962648663642895 1105 16-17 0.1915369514604066 1105 18-19 0.27068874638732154 1105 20-21 0.18295159048145848 1105 22-23 0.21471052229082233 1105 24-25 0.18816411784488452 1105 26-27 0.213678627860169 1105 28-29 0.11909913794225702 1105 30-31 0.19585479666393013 1105 32-33 0.2159130405408618 1105 34-35 0.1874872837841508 1105 36-37 0.13669433378844786 1105 38-39 0.12707435349975782 1105 40-41 0.13076709292464272 1105 42-43 0.08661466875606294 1105 44-45 0.25117964913934543 1105 46-47 0.13162729410084495 1105 48-49 0.21830654447607856 1105 50-51 0.16029271645888343 1105 52-53 0.26696693266180205 1105 54-55 0.3291439303083692 1105 56-57 0.282188352398677 1105 58-59 0.2666002166041821 1105 60-61 0.33464600571686987 1105 62-63 0.15731413533244165 1105 64-65 0.1377112026466918 1105 66-67 0.032488818349612814 1105 68-69 3.7609875393229686E-4 1105 70-71 -0.08159409145168439 1105 72-73 -0.030872257326286956 1105 74-75 0.2050407249555839 1105 76-77 0.01501628549307199 1105 78-79 0.12004892644795362 1105 80-81 0.37616645867046117 1105 82-83 0.22360611036157962 1105 84-85 0.06998822826403384 1105 86-87 0.07572219461685847 1105 88-89 0.4149930341059118 1105 90-91 0.49264410143826254 1105 92-93 0.4208281259106137 1105 94-95 0.4594123545062203 1105 96-97 0.7152337309459931 1105 98-99 0.4991917209079837 1105 100-101 0.5907205912264892 1106 1 0.3926953488574938 1106 2 0.24401375544686488 1106 3 0.19267492924128504 1106 4 0.2687632077779156 1106 5 0.21501395551616298 1106 6 0.23077219212031252 1106 7 0.2106438423076824 1106 8 0.26023111762026474 1106 9 0.33339508688781905 1106 10-11 0.3450549069850908 1106 12-13 0.40722896437623746 1106 14-15 0.5210728991209024 1106 16-17 0.4963657286263512 1106 18-19 0.5280230535855921 1106 20-21 0.5393504080863281 1106 22-23 0.614119596250255 1106 24-25 0.4629166692624196 1106 26-27 0.5421865328196347 1106 28-29 0.48515695800531944 1106 30-31 0.5268059622245858 1106 32-33 0.6581902734611091 1106 34-35 0.57067553296325 1106 36-37 0.6459297593022697 1106 38-39 0.7561336325500818 1106 40-41 0.7522739407519055 1106 42-43 0.7170044138496294 1106 44-45 0.882829716658236 1106 46-47 0.6973441709268471 1106 48-49 0.6797433934980504 1106 50-51 0.5744984734850078 1106 52-53 0.6242979815758858 1106 54-55 0.5116001516399891 1106 56-57 0.6914016939863217 1106 58-59 0.6089947477551831 1106 60-61 0.6508059186844761 1106 62-63 0.6090127874888793 1106 64-65 0.558688307769792 1106 66-67 0.6388269956654398 1106 68-69 0.6092951086699756 1106 70-71 0.765909610321188 1106 72-73 0.9381315041817899 1106 74-75 1.0314280299563308 1106 76-77 0.6797080699105109 1106 78-79 0.674632879760356 1106 80-81 0.5698999636067583 1106 82-83 0.7697216925626584 1106 84-85 0.7639081794621738 1106 86-87 0.6816924573431713 1106 88-89 0.8421120049225443 1106 90-91 0.7784997087643326 1106 92-93 1.0479414852378177 1106 94-95 1.1350406603070553 1106 96-97 1.6077207611647673 1106 98-99 1.57555323115891 1106 100-101 1.5413485349957412 1107 1 0.1137094047191951 1107 2 0.06305106758989609 1107 3 0.06592699019155646 1107 4 0.06003245570691007 1107 5 0.14082477012911454 1107 6 0.10746787889606679 1107 7 0.12049359692296235 1107 8 0.07525907258683873 1107 9 0.14901992359946092 1107 10-11 0.15708539015783884 1107 12-13 0.14522953111243453 1107 14-15 0.055102192549576046 1107 16-17 0.15301151448045403 1107 18-19 0.08594721141961514 1107 20-21 0.10003941460982446 1107 22-23 0.11837715172810448 1107 24-25 0.1741379455844978 1107 26-27 0.21236163822855048 1107 28-29 0.12870199343638689 1107 30-31 0.20154158581528492 1107 32-33 0.20577501258159003 1107 34-35 0.18511856391668857 1107 36-37 0.27357050884103984 1107 38-39 0.313139858609496 1107 40-41 0.1264740790194665 1107 42-43 0.07365884142597423 1107 44-45 0.18473682417332782 1107 46-47 0.1714441255075556 1107 48-49 0.10143883910946272 1107 50-51 0.05664377098132434 1107 52-53 0.24909449916280124 1107 54-55 0.2883754226484214 1107 56-57 0.27429339511940043 1107 58-59 0.24851836779273384 1107 60-61 0.20907825753645426 1107 62-63 0.08961714532853193 1107 64-65 0.2204372315739178 1107 66-67 0.06841052494480238 1107 68-69 0.2252253197289633 1107 70-71 0.3438494036433468 1107 72-73 0.40100726837129486 1107 74-75 0.36965046788665035 1107 76-77 0.2860811390885054 1107 78-79 0.34641524501983056 1107 80-81 0.33743160637806113 1107 82-83 0.44618666545176566 1107 84-85 0.6061379648661038 1107 86-87 0.6527381660246867 1107 88-89 0.5242659441410922 1107 90-91 0.75778017485462 1107 92-93 0.703331709239297 1107 94-95 0.7579199213795107 1107 96-97 0.7874424575147465 1107 98-99 0.8539156649894437 1107 100-101 0.8233786765190168 1108 1 -0.05797623596890844 1108 2 -0.17374187586183254 1108 3 -0.09332965071595112 1108 4 -0.005510802624186795 1108 5 0.025154341161837124 1108 6 0.11905584603484698 1108 7 0.1520810765065903 1108 8 0.17082760252679208 1108 9 0.1627181919660714 1108 10-11 0.049971960451109965 1108 12-13 0.16625351882604633 1108 14-15 0.09386932808178727 1108 16-17 0.16154880838682573 1108 18-19 0.07306214442141368 1108 20-21 0.024194115860403542 1108 22-23 0.04297593446539594 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0.057846465236224276 2211 12-13 -0.004711832600072796 2211 14-15 0.042972059879993196 2211 16-17 0.12466765054855244 2211 18-19 0.0537012421657721 2211 20-21 0.10675267119743381 2211 22-23 0.055347131544451145 2211 24-25 -0.012359353955183394 2211 26-27 0.08846755052142896 2211 28-29 0.06523220109659889 2211 30-31 0.12172443529952659 2211 32-33 0.1624260429049258 2211 34-35 0.11842277854943006 2211 36-37 0.15826643780736305 2211 38-39 0.19445024002458666 2211 40-41 0.18003254378584188 2211 42-43 0.23542419256558844 2211 44-45 0.2940705643706991 2211 46-47 0.40657663553954393 2211 48-49 0.4574149537972545 2211 50-51 0.4712940673538526 2211 52-53 0.33227426126700266 2211 54-55 0.5322425456410187 2211 56-57 0.4458155053875288 2211 58-59 0.4372773527068503 2211 60-61 0.4188236484051515 2211 62-63 0.4431550674499718 2211 64-65 0.3445247791411177 2211 66-67 0.41622742017332115 2211 68-69 0.28507721324227475 2211 70-71 0.31067203485699224 2211 72-73 0.31516793179900304 2211 74-75 0.5018154632196854 2211 76-77 0.5147207772188445 2211 78-79 0.40669195245267886 2211 80-81 0.5118047565427979 2211 82-83 0.37646831907484923 2211 84-85 0.3271648577810886 2211 86-87 0.298873157129524 2211 88-89 0.39194338871583767 2211 90-91 0.25851711731127835 2211 92-93 0.01902130389102652 2211 94-95 0.1236727395112851 2211 96-97 -0.03675039603813346 2211 98-99 -0.023435769804613926 2211 100-101 -0.3063580984053935 2212 1 0.005622966038359323 2212 2 0.23822574037372135 2212 3 0.12064204647447241 2212 4 0.04581323292607209 2212 5 0.011037058067771 2212 6 -0.060633100582897725 2212 7 -0.133353736267658 2212 8 -0.14577214770239522 2212 9 -0.10303295167944526 2212 10-11 -0.11476485890636212 2212 12-13 -0.1253775664941159 2212 14-15 -0.10548395203687022 2212 16-17 -0.10988018550083467 2212 18-19 -0.1636678142003447 2212 20-21 0.013345691259111447 2212 22-23 0.05853906756503591 2212 24-25 0.007334115222001003 2212 26-27 0.059623715865612326 2212 28-29 0.03389656821446607 2212 30-31 0.07164057509481836 2212 32-33 -0.12255293062830219 2212 34-35 -0.018160202853920282 2212 36-37 0.0849766946585575 2212 38-39 -0.04968149977874958 2212 40-41 -0.20006495068117403 2212 42-43 -0.1666676226337671 2212 44-45 -0.09949118286951375 2212 46-47 -0.09220426488008826 2212 48-49 -0.23150807347796842 2212 50-51 -0.2131528796210418 2212 52-53 -0.3201676228294801 2212 54-55 -0.37439569863224875 2212 56-57 -0.6159479262033187 2212 58-59 -0.4124163509662466 2212 60-61 -0.5838286678561317 2212 62-63 -0.45948697565707874 2212 64-65 -0.7123371295662722 2212 66-67 -0.43606332572466755 2212 68-69 -0.09106427935769545 2212 70-71 -0.16447949416802032 2212 72-73 -0.28487388663862845 2212 74-75 -0.25665849321767453 2212 76-77 -0.09999657704194931 2212 78-79 -0.25343944342332136 2212 80-81 -0.09357770628960083 2212 82-83 -0.24248838627991987 2212 84-85 -0.43623742107251573 2212 86-87 -0.41628410029562346 2212 88-89 -0.33023953372854464 2212 90-91 -0.4365142493085621 2212 92-93 -0.4987699567813273 2212 94-95 -0.3662679441894596 2212 96-97 -0.7867922703081263 2212 98-99 -0.8178945197404026 2212 100-101 -1.0886666101603417 2213 1 0.0017361699752029836 2213 2 0.13404810620573926 2213 3 0.05260497038950973 2213 4 -0.04438663991768266 2213 5 -0.10045606639877747 2213 6 -0.05503407824383544 2213 7 -0.09162122531822803 2213 8 -0.04549309205197716 2213 9 -0.10002465541764138 2213 10-11 -0.1032116910521026 2213 12-13 -0.14372449756534422 2213 14-15 -0.1085113358748302 2213 16-17 -0.17076908213996234 2213 18-19 -0.1837792856450946 2213 20-21 -0.13563684667214204 2213 22-23 -0.07347979944357519 2213 24-25 -0.045883932293342866 2213 26-27 -0.18855931183870922 2213 28-29 -0.1506100697847117 2213 30-31 -0.1531224679643941 2213 32-33 -0.11231704117788865 2213 34-35 -0.21615256688086504 2213 36-37 -0.13078785231314072 2213 38-39 -0.12230524971925405 2213 40-41 -0.193340468296185 2213 42-43 -0.10956207210801239 2213 44-45 -0.18305753387637225 2213 46-47 -0.38010736031937853 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-0.5297106969661414 2214 68-69 -0.46525084204597533 2214 70-71 -0.365492491617303 2214 72-73 -0.4869242233520694 2214 74-75 -0.43769230494341116 2214 76-77 -0.32906790305893807 2214 78-79 -0.38464789979680347 2214 80-81 -0.38565586286281217 2214 82-83 -0.4266047446544228 2214 84-85 -0.2571651276821072 2214 86-87 -0.19610271103040944 2214 88-89 -0.3966179981064819 2214 90-91 -0.6545161237632904 2214 92-93 -0.3224063089646414 2214 94-95 -0.4663665712841052 2214 96-97 -0.6561532144285884 2214 98-99 -0.6687293630011659 2214 100-101 -0.8669668945681259 2215 1 -0.13615961357290374 2215 2 -0.17817567685975533 2215 3 -0.11272198386902943 2215 4 -0.11093519684899178 2215 5 -0.18779448115929398 2215 6 -0.17329770918661325 2215 7 -0.10854932981291654 2215 8 -0.09702595368506906 2215 9 -0.12633259351632375 2215 10-11 -0.1856131352903887 2215 12-13 -0.16339296952987326 2215 14-15 -0.2883922986620533 2215 16-17 -0.30934446861282794 2215 18-19 -0.21374460373496618 2215 20-21 -0.26930255344071696 2215 22-23 -0.27270400151545715 2215 24-25 -0.347643430831738 2215 26-27 -0.2380888632802609 2215 28-29 -0.24389562571919043 2215 30-31 -0.24155189985985004 2215 32-33 -0.35555969102192364 2215 34-35 -0.4566634674080916 2215 36-37 -0.320230655888615 2215 38-39 -0.4108250080662401 2215 40-41 -0.41397093133312524 2215 42-43 -0.5991318498048059 2215 44-45 -0.6116943021182379 2215 46-47 -0.6090582397198219 2215 48-49 -0.7149556626814615 2215 50-51 -0.6955570995432581 2215 52-53 -0.6785444044195472 2215 54-55 -0.7114961145226815 2215 56-57 -0.6388411734722013 2215 58-59 -0.6926292838563839 2215 60-61 -0.8372099223719616 2215 62-63 -0.7636405208796972 2215 64-65 -0.9301904730724004 2215 66-67 -0.5704722766531702 2215 68-69 -0.6197760908432741 2215 70-71 -0.7052555127046816 2215 72-73 -0.6555188952248585 2215 74-75 -0.6043893354392722 2215 76-77 -0.7073477353252287 2215 78-79 -0.7674872038343317 2215 80-81 -0.8013409942637537 2215 82-83 -0.6560307037302522 2215 84-85 -0.7259403370064277 2215 86-87 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-1.3345950396448458 2316 54-55 -1.379717940681168 2316 56-57 -1.3684550113298002 2316 58-59 -1.2486572977490766 2316 60-61 -0.8380608500750881 2316 62-63 -0.96429174106067 2316 64-65 -1.0930892026218046 2316 66-67 -1.1220628533829071 2316 68-69 -1.1109942153291499 2316 70-71 -1.087641035051817 2316 72-73 -0.9449460499639102 2316 74-75 -0.8709428954429299 2316 76-77 -1.1929767911702136 2316 78-79 -0.9774645825017281 2316 80-81 -1.047929286010394 2316 82-83 -1.0995651155756043 2316 84-85 -1.0818700966262078 2316 86-87 -0.9916131650589257 2316 88-89 -1.2636493164513354 2316 90-91 -1.261566469314861 2316 92-93 -1.355097237142406 2316 94-95 -1.1070491449871938 2316 96-97 -1.0214692410229382 2316 98-99 -0.9710421527833368 2316 100-101 -1.0097015634509887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 14.0 5 30.0 6 73.0 7 139.0 8 352.0 9 751.0 10 1323.0 11 2081.0 12 3259.0 13 4619.0 14 5976.0 15 8183.0 16 11064.0 17 15677.0 18 13809.0 19 8120.0 20 6643.0 21 6622.0 22 6937.0 23 7450.0 24 8797.0 25 10446.0 26 12707.0 27 15939.0 28 19875.0 29 24920.0 30 31743.0 31 39984.0 32 50976.0 33 66593.0 34 92207.0 35 131143.0 36 201500.0 37 308728.0 38 238724.0 39 20127.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.049309447020335 19.60688360286996 15.239068846193499 22.10473810391621 2 15.494222533896378 21.078586170523998 39.93613099606753 23.49106029951209 3 19.36240970047891 25.37722350433648 30.36336142530775 24.897005369876858 4 12.919425816202528 17.453747192454944 37.4110187885924 32.215808202750125 5 13.51135922294857 37.67349175871043 33.918396532887115 14.896752485453879 6 30.960927977768954 36.77617886468055 17.619396413733828 14.643496743816664 7 27.643427881170695 33.60848585775067 20.927610760929454 17.82047550014918 8 26.41680096403734 34.557326828985005 19.88973096969961 19.136141237278046 9 26.58790307359496 16.731419777284145 20.527534590647097 36.1531425584738 10-11 24.533579279272413 27.140620752160466 27.322975002150574 21.002824966416547 12-13 25.93847389056782 24.60681408522071 28.093129073870678 21.36158295034079 14-15 22.790154913831902 25.53414056942085 26.981466962846813 24.69423755390043 16-17 21.51135357231006 29.002315722229483 27.407298517647398 22.07903218781306 18-19 21.33789218461896 26.996929308767804 30.604301871452012 21.06087663516123 20-21 22.305331840772183 25.942288119474583 30.888901472089252 20.863478567663982 22-23 21.858639313558953 26.115248921991373 30.704190077965066 21.3219216864846 24-25 21.760904608743722 26.213130576534894 30.841947194132473 21.18401762058891 26-27 21.677837123666304 26.432695047431338 30.640917447509743 21.248550381392615 28-29 21.328283603732707 26.43739616602402 30.902660944704518 21.33165928553876 30-31 21.573868592946855 26.284133346353972 30.80765599083134 21.33434206986783 32-33 21.454259172012577 26.440709587356526 30.64133490812924 21.46369633250165 34-35 21.782141201724993 26.31364602037805 30.577675758060824 21.32653701983613 36-37 21.894086071854556 26.257861292783836 30.394772471700072 21.453280163661535 38-39 21.641018232608314 26.4259390804168 30.57420139099364 21.35884129598125 40-41 21.74979736540544 26.296870479742168 30.541216765839348 21.41211538901305 42-43 21.709177099175683 26.4442597304547 30.237458303236647 21.60910486713297 44-45 21.85261623496497 26.543465746374135 29.882768688662992 21.721149329997903 46-47 21.944085006760254 26.797056341478907 29.401268568006316 21.857590083754523 48-49 22.151839005530157 27.040560768559562 28.97364718795416 21.83395303795612 50-51 22.133900431820962 27.16697210277608 28.799558052279483 21.899569413123483 52-53 22.34159939601603 27.302906672861376 28.57849613798711 21.77699779313549 54-55 22.04530201159574 27.237287280438277 28.666881792479575 22.05052891548641 56-57 22.102434681167 27.539914695235645 28.443705280060744 21.913945343536607 58-59 22.239423021081365 27.181166153667462 28.544921307857603 22.034489517393578 60-61 22.172946909702283 27.135593577157806 28.953569491862556 21.737890021277355 62-63 22.11812361165556 27.16694252072535 28.896148206222687 21.818785661396404 64-65 22.0190898752955 27.0812424933931 28.864716107340893 22.03495152397051 66-67 21.967101487833922 27.403098059983034 28.600196219418756 22.02960423276429 68-69 22.01322647618218 27.296158369266955 28.667770082901406 22.022845071649463 70-71 21.965310575824407 27.27717856059143 28.641206267994058 22.1163045955901 72-73 22.060492639231036 27.271232512751904 28.487967132702906 22.18030771531415 74-75 22.054559187938715 27.515232864003476 28.20160555312165 22.22860239493616 76-77 22.099252392963194 27.44719982200005 28.267618419510015 22.18592936552674 78-79 21.96157117050148 27.475956120123396 28.24214438342153 22.320328325953597 80-81 21.97746712412901 27.73431998455121 28.09226297212103 22.195949919198753 82-83 22.002752785089662 27.574311404457596 28.22679116146914 22.196144648983605 84-85 22.034843898651637 27.447201841905528 28.07406823950725 22.443886019935587 86-87 22.064343961501354 27.197508825752102 28.183604681804685 22.554542530941855 88-89 22.030702309987124 27.26489698686442 28.145175545736773 22.55922515741168 90-91 21.921882900210484 27.309479363311773 28.320670008627737 22.44796772785001 92-93 21.91322937990911 27.059359437838467 28.327888845332982 22.699522336919436 94-95 21.67627078533089 27.153195492815424 28.35116955370974 22.819364168143945 96-97 21.82170660812651 27.149739095589453 28.224971615574702 22.803582680709336 98-99 21.667456745885115 27.097224752976228 28.134249099273394 23.101069401865264 100-101 21.341046219605282 27.235945137698398 28.284277232696546 23.13873140999977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 432.0 1 340.0 2 261.5 3 333.5 4 317.5 5 279.0 6 469.5 7 1061.5 8 1796.0 9 2101.0 10 1879.5 11 1679.0 12 1749.5 13 1935.0 14 2142.0 15 2255.0 16 2419.0 17 2599.5 18 2723.0 19 2914.5 20 3211.0 21 3604.0 22 4167.0 23 4838.5 24 5832.5 25 7247.0 26 9032.0 27 11043.0 28 12809.5 29 14839.0 30 17473.0 31 20034.5 32 22434.5 33 25057.0 34 28483.0 35 31212.0 36 33988.0 37 37248.5 38 39201.5 39 40570.5 40 41887.5 41 44150.5 42 46361.5 43 47953.0 44 49566.5 45 51015.5 46 51644.5 47 51993.0 48 54449.5 49 54059.5 50 50727.5 51 48352.5 52 45955.5 53 44443.5 54 42579.0 55 39061.0 56 34962.5 57 31031.0 58 27908.5 59 25237.5 60 21872.5 61 17708.0 62 13734.5 63 10473.5 64 7726.5 65 5805.5 66 4302.0 67 3305.0 68 2397.5 69 1502.0 70 1003.5 71 717.5 72 540.5 73 404.5 74 307.5 75 234.5 76 165.0 77 80.0 78 37.0 79 29.5 80 23.5 81 14.0 82 8.0 83 5.0 84 3.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017277175254439073 2 0.002395574720153317 3 0.002976320106857151 4 1.4518634667595859E-4 5 0.0018148293334494822 6 5.08152213365855E-4 7 7.259317333797929E-5 8 0.0 9 0.0 10-11 1.0888976000696895E-4 12-13 7.985249067177723E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0030489132801951304 22-23 0.0 24-25 0.004391886986947748 26-27 0.001633346400104534 28-29 0.0029400235201881616 30-31 0.01738606501444604 32-33 5.08152213365855E-4 34-35 0.00206890544013241 36-37 0.004573369920292696 38-39 0.002577057653498265 40-41 0.004391886986947748 42-43 0.0025407610668292755 44-45 5.08152213365855E-4 46-47 1.8148293334494824E-4 48-49 1.4518634667595859E-4 50-51 1.0888976000696895E-4 52-53 0.001451863466759586 54-55 0.0037385484269059337 56-57 6.170419733728241E-4 58-59 0.001451863466759586 60-61 0.0013792702934216066 62-63 0.0 64-65 2.540761066829275E-4 66-67 3.6296586668989646E-5 68-69 0.0 70-71 1.4518634667595859E-4 72-73 3.6296586668989646E-5 74-75 6.170419733728241E-4 76-77 8.34821493386762E-4 78-79 4.7185562669686544E-4 80-81 0.00624301290706622 82-83 0.0018874225067874618 84-85 0.003883734773581892 86-87 0.002685947413505234 88-89 0.001560753226766555 90-91 3.9926245335888613E-4 92-93 0.0 94-95 4.355590400278758E-4 96-97 0.0017422361601115031 98-99 0.002976320106857151 100-101 0.001342973706752617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1377540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.36392828461226 #Duplication Level Percentage of deduplicated Percentage of total 1 81.45932501717256 51.61582828501044 2 11.314895003307313 14.339123910749645 3 3.210663050897727 6.10320669909414 4 1.3222733005137792 3.3513772234565065 5 0.6953779254668956 2.2030938499993407 6 0.42533838219837833 1.617066644778663 7 0.27598104642447396 1.2241070263486833 8 0.2021966070964818 1.0249577049162717 9 0.15686042642760972 0.8945363529767485 >10 0.842245073341128 9.777178236888409 >50 0.06514636270641411 2.8050396901409465 >100 0.028321862473332796 3.2574806771385045 >500 8.026328181194367E-4 0.29207004667329833 >1k 3.439854934797586E-4 0.4896895807408037 >5k 2.2932366231983906E-4 1.0052440710875015 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7719 0.5603467049958623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6117 0.4440524413084194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4075 0.29581718135226565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.11455202752733135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.259317333797929E-5 0.0 0.0 0.0 0.0 7 7.259317333797929E-5 0.0 0.0 0.0 0.0 8 7.259317333797929E-5 0.0 0.0 0.0 0.0 9 7.259317333797929E-5 0.0 0.0 0.0 0.0 10-11 7.259317333797929E-5 0.0 0.0 0.0 0.0 12-13 7.259317333797929E-5 0.0 0.0 0.0 0.0 14-15 1.4518634667595859E-4 0.0 0.0 0.0 0.0 16-17 1.8148293334494824E-4 0.0 0.0 7.259317333797929E-5 7.259317333797929E-5 18-19 2.177795200139379E-4 0.0 0.0 7.259317333797929E-5 7.259317333797929E-5 20-21 2.177795200139379E-4 0.0 0.0 7.259317333797929E-5 7.259317333797929E-5 22-23 2.177795200139379E-4 0.0 0.0 2.9037269335191717E-4 7.259317333797929E-5 24-25 2.177795200139379E-4 0.0 0.0 5.807453867038343E-4 7.259317333797929E-5 26-27 2.177795200139379E-4 0.0 0.0 0.0012703805334146378 7.259317333797929E-5 28-29 2.177795200139379E-4 0.0 0.0 0.0019237190934564513 7.259317333797929E-5 30-31 2.177795200139379E-4 0.0 0.0 0.0033755825602160374 7.259317333797929E-5 32-33 2.540761066829275E-4 0.0 0.0 0.0060615299737212715 7.259317333797929E-5 34-35 2.9037269335191717E-4 0.0 0.0 0.00827562176052964 7.259317333797929E-5 36-37 3.629658666898965E-4 0.0 0.0 0.011614907734076687 7.259317333797929E-5 38-39 3.629658666898965E-4 0.0 0.0 0.016696429867735237 7.259317333797929E-5 40-41 3.9926245335888613E-4 0.0 0.0 0.02185054517473177 7.259317333797929E-5 42-43 4.718556266968654E-4 0.0 0.0 0.02925504885520566 7.259317333797929E-5 44-45 5.08152213365855E-4 0.0 0.0 0.03615140032231369 7.259317333797929E-5 46-47 6.533385600418136E-4 0.0 0.0 0.04969002714984683 7.259317333797929E-5 48-49 7.25931733379793E-4 0.0 0.0 0.06573311845754025 7.259317333797929E-5 50-51 7.25931733379793E-4 0.0 0.0 0.07364577435138 7.259317333797929E-5 52-53 7.25931733379793E-4 0.0 0.0 0.08536957184546365 7.259317333797929E-5 54-55 7.25931733379793E-4 0.0 0.0 0.10536899110007694 7.259317333797929E-5 56-57 7.25931733379793E-4 0.0 0.0 0.13770924982214672 7.259317333797929E-5 58-59 7.25931733379793E-4 0.0 0.0 0.1803214425715406 7.259317333797929E-5 60-61 7.25931733379793E-4 0.0 0.0 0.22975739361470449 7.259317333797929E-5 62-63 7.25931733379793E-4 0.0 0.0 0.2844926463115408 7.259317333797929E-5 64-65 7.25931733379793E-4 0.0 0.0 0.34790278322226575 7.259317333797929E-5 66-67 7.985249067177723E-4 0.0 0.0 0.43149382232094896 7.259317333797929E-5 68-69 7.985249067177723E-4 0.0 0.0 0.5357376192342872 7.259317333797929E-5 70-71 7.985249067177723E-4 0.0 0.0 0.5899284231310887 7.259317333797929E-5 72-73 8.348214933867619E-4 0.0 0.0 0.6565326596686848 7.259317333797929E-5 74-75 8.711180800557516E-4 0.0 0.0 0.7350421766337094 7.259317333797929E-5 76-77 8.711180800557516E-4 0.0 0.0 0.8205932314125179 7.259317333797929E-5 78-79 8.711180800557516E-4 0.0 0.0 0.9068339213380374 7.259317333797929E-5 80-81 9.437112533937309E-4 0.0 0.0 1.0494432103604976 7.259317333797929E-5 82-83 9.437112533937309E-4 0.0 0.0 1.2047926013037733 7.259317333797929E-5 84-85 9.437112533937309E-4 0.0 0.0 1.4486693671327149 7.259317333797929E-5 86-87 9.800078400627205E-4 0.0 0.0 1.7936684234214613 7.259317333797929E-5 88-89 0.00101630442673171 0.0 0.0 2.1532224109644726 7.259317333797929E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 39.93476 1 GTATCAA 8010 0.0 38.672577 1 TATCAAC 10100 0.0 30.523375 2 ATCAACG 10100 0.0 30.428207 3 TCAACGC 10170 0.0 30.262178 4 CAACGCA 10300 0.0 29.972452 5 AACGCAG 10635 0.0 29.072987 6 GTACGCT 90 8.9484715E-4 26.38888 3 ACGCAGA 11990 0.0 25.7082 7 CGCAGAG 12140 0.0 25.273186 8 GCAGAGT 12650 0.0 23.878813 9 GTACATG 6680 0.0 23.115055 1 TACATGG 6760 0.0 22.696821 2 GAGTACT 7130 0.0 22.61499 12-13 ACATGGG 6705 0.0 22.173742 3 AGTACTT 7405 0.0 22.09583 12-13 GTGTACG 175 4.0425784E-6 21.71901 1 TGTACGC 110 0.0028770526 21.591686 2 GTACTTT 7580 0.0 21.085966 14-15 AGAGTAC 11410 0.0 20.479795 10-11 >>END_MODULE