FastQCFastQC Report
Thu 26 May 2016
SRR936862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126836
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55550.49297324544121773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.3472555012441917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.22824971868133426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63250.037.4194451
GGTATCA38250.034.0445561
ATCAACG76450.030.7085063
TATCAAC77150.030.6776352
TCAACGC76900.030.5288074
CAACGCA78500.029.9105515
AACGCAG80350.029.2797346
GTACATG57300.025.960811
ACGCAGA91100.025.721467
CGCAGAG90850.025.4806068
TACATGG57400.024.9220142
ACATGGG57700.023.8853383
GCAGAGT94650.023.854969
GAGTACT43650.022.97398812-13
AGTACTT45750.022.28303312-13
CATGGGG24400.022.0088754
GTACTTT45100.021.80898714-15
AGAGTAC80850.020.16510410-11
CATGGGA34700.019.0368274
TACTTTT53300.018.85492514-15
Produced by FastQC (version 0.11.5)