##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936862_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126836 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87332761821596 33.0 31.0 34.0 30.0 34.0 2 31.98067065659954 33.0 31.0 34.0 30.0 34.0 3 31.994615010525045 34.0 31.0 34.0 30.0 34.0 4 35.57766791263325 37.0 35.0 37.0 33.0 37.0 5 35.58864200291791 37.0 35.0 37.0 33.0 37.0 6 35.658905998743386 37.0 35.0 37.0 33.0 37.0 7 35.600688121430274 37.0 35.0 37.0 33.0 37.0 8 35.549425115988484 37.0 35.0 37.0 33.0 37.0 9 37.20387616299089 39.0 37.0 39.0 34.0 39.0 10-11 37.03117578778101 39.0 37.0 39.0 33.0 39.0 12-13 36.9739482941617 39.0 37.0 39.0 33.0 39.0 14-15 38.1550283271035 40.0 38.0 41.0 33.0 41.0 16-17 38.092404307281626 40.0 38.0 41.0 33.0 41.0 18-19 38.07431205605785 40.0 38.0 41.0 33.0 41.0 20-21 38.01735789413898 40.0 38.0 41.0 33.0 41.0 22-23 37.91205463794199 40.0 38.0 41.0 33.0 41.0 24-25 37.76193296983767 40.0 37.5 41.0 32.5 41.0 26-27 37.84120670621102 40.0 38.0 41.0 33.0 41.0 28-29 37.79150071527711 40.0 38.0 41.0 33.0 41.0 30-31 37.644872457039 40.0 37.0 41.0 32.5 41.0 32-33 37.43692737896198 40.0 37.0 41.0 32.0 41.0 34-35 37.27567764963136 40.0 36.5 41.0 31.5 41.0 36-37 37.105685743089495 40.0 36.0 41.0 31.0 41.0 38-39 36.94716223123862 39.0 36.0 41.0 31.0 41.0 40-41 36.769504612916165 39.0 36.0 41.0 30.5 41.0 42-43 36.55138147876 39.0 35.5 41.0 30.0 41.0 44-45 36.27868518577681 39.0 35.0 41.0 30.0 41.0 46-47 35.907433734811455 39.0 35.0 40.5 28.5 41.0 48-49 35.57335716998747 39.0 35.0 40.0 27.5 41.0 50-51 34.91108999002517 38.0 34.0 39.5 26.5 40.5 52-53 35.13792290981119 38.5 35.0 40.0 27.0 40.5 54-55 35.513434075588634 39.0 35.0 40.5 27.5 41.0 56-57 35.42122411779532 39.0 35.0 41.0 27.0 41.0 58-59 35.18970906147834 39.0 35.0 41.0 26.5 41.0 60-61 34.922048993819864 38.0 34.5 40.0 26.0 41.0 62-63 34.60636641001885 38.0 34.0 40.0 26.0 41.0 64-65 34.29545603796826 37.5 34.0 40.0 25.5 41.0 66-67 33.93527097110849 37.0 34.0 40.0 24.5 41.0 68-69 33.557186671352355 36.0 33.5 39.0 23.0 41.0 70-71 33.1479585316763 36.0 33.0 39.0 22.5 41.0 72-73 32.74819050864545 35.0 33.0 38.5 22.0 40.0 74-75 32.32522345753952 35.0 32.0 37.5 20.5 39.5 76-77 31.897570720140287 35.0 32.0 37.0 20.0 39.0 78-79 31.46798203110302 35.0 31.5 36.5 18.0 39.0 80-81 31.042977416411972 35.0 31.0 36.0 16.5 38.5 82-83 30.646850118384574 35.0 31.0 35.5 12.0 37.0 84-85 30.302898114721216 34.0 31.0 35.0 7.5 37.0 86-87 29.969485799175743 34.0 30.5 35.0 6.5 36.0 88-89 29.635900432716028 34.0 30.0 35.0 2.0 36.0 90-91 29.299888803694593 34.0 30.0 35.0 2.0 36.0 92-93 28.957766258798973 34.0 29.0 35.0 2.0 35.0 94-95 28.565900894185134 34.0 29.0 35.0 2.0 35.0 96-97 28.122112268333638 34.0 29.0 35.0 2.0 35.0 98-99 27.55777770678253 34.0 28.0 35.0 2.0 35.0 100-101 25.408325612600237 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34947572127727966 1101 2 0.26693320806526444 1101 3 0.3306889568497162 1101 4 0.19412965049047415 1101 5 0.18694006129771168 1101 6 0.026746382578672012 1101 7 0.17841278420167583 1101 8 0.15636619373379546 1101 9 0.017787866405640784 1101 10-11 0.14968690573139298 1101 12-13 0.24641475642470567 1101 14-15 0.4614110647835332 1101 16-17 0.6488171524648862 1101 18-19 0.4064920023043399 1101 20-21 0.47076677767370967 1101 22-23 0.5812453811367391 1101 24-25 0.4502495001842064 1101 26-27 0.5924055434359161 1101 28-29 0.5982623496666903 1101 30-31 0.44276808720654515 1101 32-33 0.5872639597588076 1101 34-35 0.3324008468065003 1101 36-37 0.3997091960793355 1101 38-39 0.5273624190679342 1101 40-41 0.3860670832017661 1101 42-43 0.4685126527973793 1101 44-45 0.7145682067355992 1101 46-47 0.509416274160067 1101 48-49 0.7667361980110314 1101 50-51 0.6791246190553792 1101 52-53 0.6718723971874425 1101 54-55 0.80372816907024 1101 56-57 0.7826698418119875 1101 58-59 0.6313738445048287 1101 60-61 0.7314349289588975 1101 62-63 0.8091071850427412 1101 64-65 0.8430349107150406 1101 66-67 0.8013381650210505 1101 68-69 0.7758218187779917 1101 70-71 0.8058026703918557 1101 72-73 0.5885183812236292 1101 74-75 0.3197349425816185 1101 76-77 0.4650900498745365 1101 78-79 0.4924985734782119 1101 80-81 0.33652106516884217 1101 82-83 0.3807935418999904 1101 84-85 0.5639896635056836 1101 86-87 0.4787597935908643 1101 88-89 0.5119862466166012 1101 90-91 0.6797044651233435 1101 92-93 0.6299161953803747 1101 94-95 0.4434943353978156 1101 96-97 0.38417985524287346 1101 98-99 0.49363483172230715 1101 100-101 0.6065799266994354 1102 1 0.2859901677070553 1102 2 0.16665885986825302 1102 3 0.24103594403310424 1102 4 0.22839687451081403 1102 5 0.14883788015557542 1102 6 0.10537533198451854 1102 7 0.17459508685261227 1102 8 0.2789960570857133 1102 9 0.22943213098316306 1102 10-11 0.38784423595686945 1102 12-13 0.29906569313722287 1102 14-15 0.2730872188459088 1102 16-17 0.42640155686300574 1102 18-19 0.3984337431743299 1102 20-21 0.25004880400389595 1102 22-23 0.25584976248894975 1102 24-25 0.4682682919672416 1102 26-27 0.3561887048016388 1102 28-29 0.4323153881264048 1102 30-31 0.38451060098559253 1102 32-33 0.4098840425728483 1102 34-35 0.2689641959256761 1102 36-37 0.17134103362049302 1102 38-39 0.353284460783982 1102 40-41 0.2771106583746459 1102 42-43 0.38415592452701475 1102 44-45 0.3980856169149263 1102 46-47 0.30127451060580057 1102 48-49 0.4884322254688698 1102 50-51 0.421577988902726 1102 52-53 0.4270873142336882 1102 54-55 0.44707551295559256 1102 56-57 0.6511963996708516 1102 58-59 0.6513347716669387 1102 60-61 0.42086545508608 1102 62-63 0.41325470842974 1102 64-65 0.6467822539428809 1102 66-67 0.3109781590376599 1102 68-69 0.1659062437234482 1102 70-71 0.4113178252942049 1102 72-73 0.38087016498004544 1102 74-75 0.2459929745401439 1102 76-77 0.3136507492076426 1102 78-79 0.248765103820066 1102 80-81 0.3659950037339108 1102 82-83 0.2724305244972065 1102 84-85 0.5446229656618371 1102 86-87 0.46013690976228716 1102 88-89 0.28032598798301933 1102 90-91 0.35652919295316465 1102 92-93 0.345028852382665 1102 94-95 0.4035309754296392 1102 96-97 0.3717022724000749 1102 98-99 0.3356014759786632 1102 100-101 0.4071822927677111 1103 1 0.2252276778431863 1103 2 0.35818989390966394 1103 3 0.3450838295282672 1103 4 0.22118979930007043 1103 5 0.3166379412881426 1103 6 0.25676643475375727 1103 7 0.16433854057331843 1103 8 0.1358767972108197 1103 9 0.2228494331843578 1103 10-11 0.28176807117512226 1103 12-13 0.20109654066535398 1103 14-15 0.26451160770967164 1103 16-17 0.2939828122363153 1103 18-19 0.21085148576580082 1103 20-21 0.45391960713934054 1103 22-23 0.4062451453800833 1103 24-25 0.37567602683714796 1103 26-27 0.3661747436721896 1103 28-29 0.4853782056305249 1103 30-31 0.292947948101542 1103 32-33 0.4363539819370814 1103 34-35 0.2699641260358234 1103 36-37 0.2028594622271669 1103 38-39 0.34403268638169493 1103 40-41 0.1849145760797697 1103 42-43 0.3912326930560397 1103 44-45 0.5177801982620807 1103 46-47 0.4679096005321455 1103 48-49 0.5150053155181524 1103 50-51 0.4492775265350417 1103 52-53 0.37960915902263537 1103 54-55 0.43543169521822733 1103 56-57 0.5190551093357811 1103 58-59 0.6100734450901939 1103 60-61 0.5333483299672466 1103 62-63 0.5699742808722519 1103 64-65 0.4474172411395685 1103 66-67 0.507022772340477 1103 68-69 0.5948543677703597 1103 70-71 0.5175916604703588 1103 72-73 0.3921493766225126 1103 74-75 0.21009716767864006 1103 76-77 0.4377309187621101 1103 78-79 0.37942818168493986 1103 80-81 0.30616739589513386 1103 82-83 0.475613796740074 1103 84-85 0.43269121015506684 1103 86-87 0.3285197933165307 1103 88-89 0.47927608241852226 1103 90-91 0.5095606021262498 1103 92-93 0.4674687155332826 1103 94-95 0.4992996501243745 1103 96-97 0.6485027149796672 1103 98-99 0.6803963240019186 1103 100-101 0.5044077077492304 1104 1 0.3997201336634362 1104 2 0.20875658742006564 1104 3 0.2417313603815323 1104 4 0.3911460726265261 1104 5 0.3419541727547255 1104 6 0.23830784655255854 1104 7 0.2944361818220145 1104 8 0.33660979924431444 1104 9 0.2529748265858842 1104 10-11 0.48129151538534387 1104 12-13 0.485878822528214 1104 14-15 0.5946650257892685 1104 16-17 0.5924342784447774 1104 18-19 0.5348328886439333 1104 20-21 0.5478420219309257 1104 22-23 0.548873042607184 1104 24-25 0.6557729186414463 1104 26-27 0.5769977653320382 1104 28-29 0.5177875875841735 1104 30-31 0.6419912255626627 1104 32-33 0.6430886709017685 1104 34-35 0.5998391141696189 1104 36-37 0.7302016916925069 1104 38-39 0.8010304757478508 1104 40-41 0.7773031280214937 1104 42-43 0.757547257122404 1104 44-45 0.8079416740043825 1104 46-47 0.7259544318412949 1104 48-49 0.8815503905102844 1104 50-51 0.8896998770560813 1104 52-53 0.8474055336194724 1104 54-55 0.9335455935054497 1104 56-57 0.8561720013126788 1104 58-59 0.9963520721625727 1104 60-61 0.9687614396118747 1104 62-63 1.040937852878642 1104 64-65 1.017406944227865 1104 66-67 1.0401984044059844 1104 68-69 0.8750854782516413 1104 70-71 1.0035277687464514 1104 72-73 0.8201577485976514 1104 74-75 0.7443672156567089 1104 76-77 0.753675552535892 1104 78-79 0.842845530005345 1104 80-81 0.8439600596067187 1104 82-83 0.870647524008632 1104 84-85 1.0461089445528842 1104 86-87 1.0357136872978572 1104 88-89 0.858617806803764 1104 90-91 0.8311040396959406 1104 92-93 0.9231919952291072 1104 94-95 1.1492126010230521 1104 96-97 1.1578789456014213 1104 98-99 1.1446010431396871 1104 100-101 0.9940912650755926 1105 1 0.223838117308496 1105 2 0.2831282517506075 1105 3 0.2248428131639919 1105 4 0.22189577366410163 1105 5 0.24721961640261725 1105 6 0.243255906332692 1105 7 0.12703831788542175 1105 8 0.25025956964528717 1105 9 0.3993460092833203 1105 10-11 0.4250793745261845 1105 12-13 0.15059400035168125 1105 14-15 0.29989913241131916 1105 16-17 0.1882471063515041 1105 18-19 0.2705876632952169 1105 20-21 0.2141644444219537 1105 22-23 0.35355229348246553 1105 24-25 0.30695322849207685 1105 26-27 0.2348694821482411 1105 28-29 0.2081108786614081 1105 30-31 0.00908132800231698 1105 32-33 -0.0031762540643214265 1105 34-35 0.0777555158351646 1105 36-37 0.011694888110305612 1105 38-39 0.014002357842414881 1105 40-41 0.2200826964864646 1105 42-43 0.20887041029614295 1105 44-45 0.37658905682243216 1105 46-47 0.2739121736818433 1105 48-49 0.4638296368573833 1105 50-51 0.23071129628888798 1105 52-53 0.17818482216092946 1105 54-55 0.2153930681219407 1105 56-57 0.46781002727558274 1105 58-59 0.30939403804894994 1105 60-61 0.4655613581047291 1105 62-63 0.49616115362970703 1105 64-65 0.5040966840019081 1105 66-67 0.5557596304709733 1105 68-69 0.767845699061894 1105 70-71 0.5030811260791097 1105 72-73 0.5438861034089442 1105 74-75 0.6883253959068725 1105 76-77 0.3215145532912693 1105 78-79 0.2859940417796274 1105 80-81 0.4926267180403414 1105 82-83 0.48851462002857104 1105 84-85 0.4139575162225668 1105 86-87 0.41730166664114776 1105 88-89 0.2364209011935614 1105 90-91 0.3294005713200967 1105 92-93 0.33096491551059515 1105 94-95 0.2306055086668124 1105 96-97 0.37211151264412834 1105 98-99 0.34451222917150304 1105 100-101 0.4676186821431081 1106 1 0.16960699939813395 1106 2 0.1726474879679536 1106 3 0.13603466539357711 1106 4 0.11403555495482465 1106 5 0.2798914391687859 1106 6 0.14582829003715148 1106 7 0.16944157019433703 1106 8 0.19625509347099523 1106 9 0.12130617699820334 1106 10-11 0.23783434647926782 1106 12-13 0.3374388640833388 1106 14-15 0.3056820379583698 1106 16-17 0.323219342738831 1106 18-19 0.24017163405842723 1106 20-21 0.35571637370973264 1106 22-23 0.30508637473351996 1106 24-25 0.3409707365661845 1106 26-27 0.2518746949081674 1106 28-29 0.22957810060032813 1106 30-31 0.1100820929181765 1106 32-33 0.21520368247599464 1106 34-35 0.16235662637966897 1106 36-37 0.327654715862586 1106 38-39 0.1790258719225335 1106 40-41 0.058497762495868244 1106 42-43 0.11476805322208605 1106 44-45 0.02693751849164272 1106 46-47 0.1387274606669351 1106 48-49 0.22646845492073453 1106 50-51 0.2951589987082883 1106 52-53 0.24927950174617308 1106 54-55 0.39463480986368893 1106 56-57 0.40770747189001355 1106 58-59 0.387503987621173 1106 60-61 0.37873278424785184 1106 62-63 0.4593956145056737 1106 64-65 0.39854537797890544 1106 66-67 0.4284861305559673 1106 68-69 0.43381119285756853 1106 70-71 0.3449099832381535 1106 72-73 0.5134311201237693 1106 74-75 0.51355628717549 1106 76-77 0.57653254297907 1106 78-79 0.5360294545508886 1106 80-81 0.4807988524388911 1106 82-83 0.5019573079995645 1106 84-85 0.49316172042676953 1106 86-87 0.5069101430420773 1106 88-89 0.39104368435408077 1106 90-91 0.3641086284338151 1106 92-93 0.4576080680971444 1106 94-95 0.5277328246620527 1106 96-97 0.5084577645564217 1106 98-99 0.36697809126000536 1106 100-101 0.2684289263495785 1107 1 0.20073781700819637 1107 2 0.20985377727613397 1107 3 0.2417088792300568 1107 4 0.07466574363407119 1107 5 0.11551030709330945 1107 6 0.24578803846482344 1107 7 0.21608936893647268 1107 8 0.201687797873511 1107 9 0.16779045372776125 1107 10-11 0.39487396912078054 1107 12-13 0.2419495559072402 1107 14-15 0.11463486814704993 1107 16-17 0.30636902827342993 1107 18-19 0.25067414978170177 1107 20-21 0.4228635435210535 1107 22-23 0.31596310429327445 1107 24-25 0.3628487239875753 1107 26-27 0.21275897163772584 1107 28-29 0.27047244022296724 1107 30-31 0.2642651117861021 1107 32-33 0.26543575794768515 1107 34-35 0.43741197229162054 1107 36-37 0.39781320642862283 1107 38-39 0.039237192677248345 1107 40-41 0.36221663042039154 1107 42-43 0.433373713599444 1107 44-45 0.5096683361017114 1107 46-47 0.46056142293108593 1107 48-49 0.4920200272477757 1107 50-51 0.4661533383309262 1107 52-53 0.6205109482870554 1107 54-55 0.7224681431970197 1107 56-57 0.671184201449762 1107 58-59 0.658463107118024 1107 60-61 0.877193790537163 1107 62-63 0.7916572497257945 1107 64-65 0.8227177050229315 1107 66-67 0.8025578286691726 1107 68-69 0.7581105639267562 1107 70-71 0.5695760209739973 1107 72-73 0.6932650823879243 1107 74-75 0.9616971676786363 1107 76-77 0.6708665052432288 1107 78-79 0.6745099576955447 1107 80-81 0.616577468791089 1107 82-83 0.6353296350435862 1107 84-85 0.6135617204267696 1107 86-87 0.5298019669414451 1107 88-89 0.6780845648572225 1107 90-91 0.6881230938426164 1107 92-93 0.8918634140090944 1107 94-95 0.9272397429010475 1107 96-97 0.8601043054369235 1107 98-99 0.7513026195618906 1107 100-101 0.7146415049659289 1108 1 0.1165650278925483 1108 2 0.17600071605164302 1108 3 0.20247350508039474 1108 4 0.1723079205048137 1108 5 0.08671983090283675 1108 6 0.03185062349884049 1108 7 0.11091249818817772 1108 8 0.1579095665809973 1108 9 0.04277004556449526 1108 10-11 0.09492907116159444 1108 12-13 0.2507924130500996 1108 14-15 0.32857704501780205 1108 16-17 0.2900159670489373 1108 18-19 0.37569659876129435 1108 20-21 0.2116478972625444 1108 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58-59 -1.4553099799532134 2316 60-61 -1.4892093756897467 2316 62-63 -1.5600074820244245 2316 64-65 -1.764460131388681 2316 66-67 -1.4748240534768229 2316 68-69 -1.5459393920978712 2316 70-71 -1.747582466273144 2316 72-73 -1.745164820866254 2316 74-75 -2.060791134871934 2316 76-77 -2.3022810761112176 2316 78-79 -2.24279100976268 2316 80-81 -2.4243885822203417 2316 82-83 -2.254715747542164 2316 84-85 -2.229133794085101 2316 86-87 -2.57406469972522 2316 88-89 -2.548464247807683 2316 90-91 -2.2524084804757614 2316 92-93 -2.3453386968088488 2316 94-95 -2.8781681726662356 2316 96-97 -3.0019822380986945 2316 98-99 -2.967603976744172 2316 100-101 -3.1256754695283533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2184.0 3 748.0 4 751.0 5 758.0 6 900.0 7 1076.0 8 1266.0 9 1684.0 10 2054.0 11 2557.0 12 3489.0 13 4382.0 14 5082.0 15 6390.0 16 8283.0 17 11227.0 18 10045.0 19 6112.0 20 5726.0 21 5985.0 22 6298.0 23 7017.0 24 7927.0 25 9055.0 26 10692.0 27 12715.0 28 15323.0 29 18642.0 30 22975.0 31 29087.0 32 37087.0 33 49509.0 34 68400.0 35 99128.0 36 152798.0 37 234218.0 38 223471.0 39 41776.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32852398062054 19.032056728521496 14.44892204622554 22.190497244632425 2 15.214656497224297 20.68412020444237 40.74864544057473 23.352577857758607 3 19.20982389737244 25.68795775454282 29.945790677629848 25.1564276704549 4 12.622426393225894 17.046246567266483 37.789590415958955 32.54173662354866 5 13.595676436007913 37.61255581169834 33.98741506143283 14.804352690860911 6 30.697298545370582 36.66687387883417 18.096637834573645 14.539189741221605 7 27.763944046206635 33.456120034953365 21.059132986167846 17.720802932672157 8 26.175064051614278 34.54329089867635 20.182408340696863 19.09923670901252 9 26.669834024527784 17.06190556537338 20.696493113216764 35.57176729688208 10-11 24.459893675032106 27.241598888792183 27.483210211175667 20.815297225000045 12-13 25.853928218563706 24.78393410228799 27.952486151045193 21.40965152810311 14-15 22.783122231719634 25.738441215323647 26.78729616020781 24.69114039274891 16-17 21.731619529057525 29.22698997547924 27.096485179878854 21.944905315584382 18-19 21.571412970034796 27.516108439072944 29.9157930626056 20.99668552828666 20-21 22.604282977528463 26.555126631311865 29.879528770358792 20.961061620800876 22-23 22.17526161232019 26.616856462099502 29.843511978950282 21.36436994663002 24-25 21.976837383270478 26.790474001081087 29.93366132838885 21.299027287259584 26-27 21.777045234265994 26.97564854952834 29.83523096422371 21.41207525198195 28-29 21.454381010031437 27.100770597643653 29.957626892987932 21.48722149933698 30-31 21.758042792827776 26.83824328851987 29.925246792091116 21.47846712656124 32-33 21.537404726489143 27.024706325060617 29.829217683331617 21.608671265118623 34-35 21.958003725862437 26.978199533335285 29.631966799175302 21.431829941626972 36-37 21.95392852673702 26.87684542821922 29.596342113881168 21.572883931162586 38-39 21.825633670690486 27.00806790512701 29.696822316603928 21.46947610757858 40-41 21.86889561225151 26.914730938778842 29.69751080762298 21.51886264134667 42-43 21.80381263423206 27.123662116828484 29.402367809155294 21.670157439784163 44-45 21.921825448417213 27.21324394348898 29.1516114532476 21.713319154846207 46-47 22.005922925644832 27.4024501068505 28.75955797260935 21.832068994895316 48-49 22.19144249678743 27.47514252447587 28.44161998679474 21.891794991941953 50-51 22.083266032052567 27.819687000232523 28.2455487968306 21.851498170884312 52-53 22.25034556358481 27.863274411876294 28.09823546247834 21.78814456206056 54-55 21.99421610057613 27.76671102734994 28.167377191982062 22.071695680091874 56-57 22.039953860130865 28.150202228081678 27.889189751765198 21.920654160022263 58-59 22.221152267392068 27.788832334133577 28.010490488002947 21.979524910471408 60-61 22.18093281513722 27.59580703186267 28.46284019934307 21.760419953657042 62-63 22.169446455032045 27.697973105808288 28.388900254687577 21.74368018447209 64-65 21.970622822782847 27.70968958707759 28.37591248973796 21.943775100401606 66-67 21.967193907959924 27.94824126914247 28.077429513411655 22.00713530948595 68-69 21.83673886046881 27.87024289179984 28.290951763391092 22.002066484340258 70-71 21.90116663789312 27.84943549850947 28.188593514989833 22.060804348607572 72-73 21.928871156359634 27.832605121081468 28.057393037122182 22.181130685436717 74-75 21.79937521940326 28.16403353169681 27.910180355580845 22.12641089331909 76-77 22.036801309500984 28.058979029520582 27.83053537570038 22.073684285278055 78-79 21.915059745464212 28.126555004489955 27.788754207528655 22.169631042517178 80-81 21.88389393414827 28.31859703320994 27.726437455058722 22.07107157758307 82-83 21.935674871459817 28.14381941879953 27.796725421128883 22.123780288611766 84-85 21.915914396541776 27.995277785174732 27.73391384620847 22.354893972075025 86-87 22.105781734742337 27.81512828820352 27.824404273166664 22.254685703887482 88-89 22.079066167477052 27.74390081535487 27.780825225052503 22.396207792115575 90-91 21.86447229617611 27.847525452884035 27.97596038842914 22.31204186251071 92-93 21.966770855433868 27.73641987747685 27.92208656870378 22.3747226983855 94-95 21.76409053769349 27.771117998225602 27.9037316907225 22.561059773358405 96-97 21.79089835669706 27.915686396363604 27.74882589511626 22.54458935182308 98-99 21.625998934469898 27.905167820990943 27.686112591014027 22.78272065352513 100-101 21.728008709887828 27.952763422378496 27.76552037095171 22.553707496781968 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 197.0 2 163.5 3 200.0 4 208.0 5 230.5 6 362.0 7 697.0 8 1218.0 9 1534.5 10 1464.5 11 1389.5 12 1451.5 13 1494.0 14 1574.0 15 1706.0 16 1770.5 17 1856.0 18 2027.5 19 2198.5 20 2379.0 21 2693.5 22 3137.5 23 3780.0 24 4620.5 25 5779.0 26 7340.5 27 9016.0 28 10460.0 29 12287.0 30 14570.0 31 16638.5 32 18750.5 33 20967.0 34 23918.5 35 26163.5 36 28143.5 37 31104.0 38 32922.0 39 33579.0 40 34510.0 41 36535.5 42 38168.0 43 39479.5 44 41030.5 45 42366.5 46 42704.0 47 42584.5 48 44836.0 49 44847.5 50 41886.0 51 39734.5 52 37580.5 53 36088.0 54 34452.5 55 31565.5 56 28309.0 57 25115.0 58 22575.0 59 20029.5 60 16928.5 61 13751.5 62 10861.0 63 8299.5 64 6172.0 65 4657.5 66 3313.0 67 2479.5 68 1809.5 69 1192.0 70 819.0 71 572.5 72 426.0 73 318.5 74 230.0 75 179.5 76 119.5 77 58.5 78 32.5 79 16.5 80 13.5 81 13.5 82 10.5 83 10.0 84 8.0 85 4.0 86 5.5 87 5.5 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0433958446482008 2 0.04037854665630136 3 0.024582104228121928 4 0.04854300004614691 5 0.06460567465008217 6 0.0688653894621755 7 0.06780046075915218 8 0.07019655034095468 9 0.06797794887632273 10-11 0.07578742603182717 12-13 0.07529933370960813 14-15 0.07086213078034426 16-17 0.057461777933967326 18-19 0.04996290498351135 20-21 0.05621936111377343 22-23 0.031947861090699976 24-25 0.0171276033069586 26-27 0.014909001842326658 28-29 0.015796442428179434 30-31 0.011403611528208187 32-33 0.0070995246868222174 34-35 0.010383054854477493 36-37 0.005768363808043052 38-39 0.007765105126211799 40-41 0.007543244979748606 42-43 0.004969667280775552 44-45 0.0010205566737306938 46-47 0.002484833640387776 48-49 0.0010649287030233326 50-51 0.005502131632287219 52-53 0.004747807134312358 54-55 0.007765105126211799 56-57 0.023206571320050124 58-59 0.008963149917113049 60-61 0.020899225796832902 62-63 0.01433216546152235 64-65 0.009850590502965826 66-67 0.016462022867569016 68-69 0.02529205669680415 70-71 0.01974555303522429 72-73 0.019390576800883182 74-75 0.017793183746348182 76-77 0.026623217575583315 78-79 0.03838180533813261 80-81 0.03248032544221165 82-83 0.030172979918994425 84-85 0.021831038411978317 86-87 0.02404963987661026 88-89 0.018636252302908322 90-91 0.017349463453421792 92-93 0.03150414079777359 94-95 0.022674106968538457 96-97 0.03957985012903386 98-99 0.05644122126023663 100-101 0.06740111249551843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.48958625209058 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92180510319157 56.638479039429825 2 10.012193161338717 13.514375478695213 3 2.668989168333628 5.403869240464436 4 1.1173941195317778 3.0164986723087486 5 0.5604608318403541 1.8912634825704002 6 0.3671721148404142 1.486817646833078 7 0.24540262124236267 1.1593484960977885 8 0.16515144597237139 0.8916802206087868 9 0.1280534050302411 0.7778044191296111 >10 0.7408698232923385 9.198811280837297 >50 0.05116983984066835 2.3527575809749623 >100 0.020416339484316974 2.3515677916004236 >500 3.951540262895795E-4 0.15005634525780623 >1k 3.951540262895795E-4 0.6728533633035162 >5k 1.3171800876319315E-4 0.49381694188810044 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5555 0.49297324544121773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3913 0.3472555012441917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.22824971868133426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.874405858527772E-5 0.0 0.0 0.0 0.0 6 8.874405858527772E-5 0.0 0.0 0.0 0.0 7 1.7748811717055544E-4 0.0 0.0 0.0 0.0 8 1.7748811717055544E-4 0.0 0.0 0.0 0.0 9 1.7748811717055544E-4 0.0 0.0 0.0 0.0 10-11 1.7748811717055544E-4 0.0 0.0 0.0 0.0 12-13 1.7748811717055544E-4 0.0 0.0 0.0 0.0 14-15 2.218601464631943E-4 0.0 0.0 1.3311608787791658E-4 8.874405858527772E-5 16-17 2.6623217575583315E-4 0.0 0.0 2.218601464631943E-4 8.874405858527772E-5 18-19 2.6623217575583315E-4 0.0 0.0 2.6623217575583315E-4 8.874405858527772E-5 20-21 2.6623217575583315E-4 0.0 0.0 3.5497623434111087E-4 8.874405858527772E-5 22-23 2.6623217575583315E-4 0.0 0.0 4.437202929263886E-4 8.874405858527772E-5 24-25 2.6623217575583315E-4 0.0 0.0 5.324643515116663E-4 8.874405858527772E-5 26-27 2.6623217575583315E-4 0.0 0.0 9.761846444380548E-4 8.874405858527772E-5 28-29 2.6623217575583315E-4 0.0 0.0 0.002262973493924582 8.874405858527772E-5 30-31 2.6623217575583315E-4 0.0 0.0 0.005147155397946107 8.874405858527772E-5 32-33 2.6623217575583315E-4 0.0 0.0 0.007898221214089717 8.874405858527772E-5 34-35 2.6623217575583315E-4 0.0 0.0 0.01047179891306277 8.874405858527772E-5 36-37 2.6623217575583315E-4 0.0 0.0 0.014953373871619297 8.874405858527772E-5 38-39 2.6623217575583315E-4 0.0 0.0 0.01992304115239485 8.874405858527772E-5 40-41 2.6623217575583315E-4 0.0 0.0 0.0264901014877054 8.874405858527772E-5 42-43 2.6623217575583315E-4 0.0 0.0 0.03443269473108775 8.874405858527772E-5 44-45 2.6623217575583315E-4 0.0 0.0 0.04108849912498358 8.874405858527772E-5 46-47 2.6623217575583315E-4 0.0 0.0 0.05630810517235871 8.874405858527772E-5 48-49 2.6623217575583315E-4 0.0 0.0 0.07600928617829036 8.874405858527772E-5 50-51 2.6623217575583315E-4 0.0 0.0 0.084040623480258 8.874405858527772E-5 52-53 2.6623217575583315E-4 0.0 0.0 0.0971747441508791 8.874405858527772E-5 54-55 2.6623217575583315E-4 0.0 0.0 0.116476576893177 8.874405858527772E-5 56-57 2.6623217575583315E-4 0.0 0.0 0.15184108423941017 8.874405858527772E-5 58-59 2.6623217575583315E-4 0.0 0.0 0.20215896545726264 8.874405858527772E-5 60-61 2.6623217575583315E-4 0.0 0.0 0.26210557703161774 8.874405858527772E-5 62-63 2.6623217575583315E-4 0.0 0.0 0.33429886869074116 8.874405858527772E-5 64-65 2.6623217575583315E-4 0.0 0.0 0.4113730835720548 8.874405858527772E-5 66-67 2.6623217575583315E-4 0.0 0.0 0.5148042838531961 8.874405858527772E-5 68-69 2.6623217575583315E-4 0.0 0.0 0.6492071605805991 8.874405858527772E-5 70-71 2.6623217575583315E-4 0.0 0.0 0.7131028827619991 8.874405858527772E-5 72-73 3.5497623434111087E-4 0.0 0.0 0.7896446332918011 8.874405858527772E-5 74-75 3.5497623434111087E-4 0.0 0.0 0.882426546542709 8.874405858527772E-5 76-77 3.5497623434111087E-4 0.0 0.0 0.9884313245228231 8.874405858527772E-5 78-79 3.5497623434111087E-4 0.0 0.0 1.089821411456503 8.874405858527772E-5 80-81 3.5497623434111087E-4 0.0 0.0 1.2569708458018736 8.874405858527772E-5 82-83 3.5497623434111087E-4 0.0 0.0 1.4431115086844937 8.874405858527772E-5 84-85 4.437202929263886E-4 4.437202929263886E-5 0.0 1.7201704595877305 8.874405858527772E-5 86-87 4.437202929263886E-4 8.874405858527772E-5 0.0 2.1351820495617817 8.874405858527772E-5 88-89 5.324643515116663E-4 8.874405858527772E-5 0.0 2.558358092925679 8.874405858527772E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6325 0.0 37.419445 1 GGTATCA 3825 0.0 34.044556 1 ATCAACG 7645 0.0 30.708506 3 TATCAAC 7715 0.0 30.677635 2 TCAACGC 7690 0.0 30.528807 4 CAACGCA 7850 0.0 29.910551 5 AACGCAG 8035 0.0 29.279734 6 GTACATG 5730 0.0 25.96081 1 ACGCAGA 9110 0.0 25.72146 7 CGCAGAG 9085 0.0 25.480606 8 TACATGG 5740 0.0 24.922014 2 ACATGGG 5770 0.0 23.885338 3 GCAGAGT 9465 0.0 23.85496 9 GAGTACT 4365 0.0 22.973988 12-13 AGTACTT 4575 0.0 22.283033 12-13 CATGGGG 2440 0.0 22.008875 4 GTACTTT 4510 0.0 21.808987 14-15 AGAGTAC 8085 0.0 20.165104 10-11 CATGGGA 3470 0.0 19.036827 4 TACTTTT 5330 0.0 18.854925 14-15 >>END_MODULE