FastQCFastQC Report
Thu 26 May 2016
SRR936862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126836
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.6117127958283193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53890.4782417317160616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.31166913375149535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.13089748641328464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.038.012111
GTATCAA66450.037.4686741
TATCAAC83400.029.7900522
ATCAACG83700.029.5684533
TCAACGC84450.029.189474
CAACGCA86000.028.6633825
AACGCAG88750.027.7727556
TAACACG950.001227117824.9979785
TAGACCG1151.2824347E-424.7806075
ACGCAGA99450.024.736887
CGCAGAG99750.024.4244298
GCAGAGT104050.023.0499119
GAGTACT59300.022.5461612-13
GTACATG53500.021.6704561
AGTACTT60550.021.13944212-13
GTACTTT63150.020.9097714-15
TACATGG54200.020.6846582
ACATGGG53650.020.2759913
AGAGTAC93100.019.97110410-11
CAGAGTA102150.018.78291310-11
Produced by FastQC (version 0.11.5)