##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126836 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23677358550845 33.0 31.0 34.0 31.0 34.0 2 32.30998033431662 33.0 31.0 34.0 31.0 34.0 3 32.4398617012591 34.0 31.0 34.0 31.0 34.0 4 36.11402102879212 37.0 35.0 37.0 35.0 37.0 5 36.02909296472601 37.0 35.0 37.0 35.0 37.0 6 36.06649681053853 37.0 35.0 37.0 35.0 37.0 7 36.02466374876202 37.0 35.0 37.0 35.0 37.0 8 36.00483921351466 37.0 35.0 37.0 35.0 37.0 9 37.70850594052728 39.0 37.0 39.0 35.0 39.0 10-11 37.61202694979571 39.0 37.0 39.0 35.0 39.0 12-13 37.53695879435872 39.0 37.0 39.0 35.0 39.0 14-15 38.7702363076792 40.0 38.0 41.0 35.0 41.0 16-17 38.69891537011597 40.0 38.0 41.0 35.0 41.0 18-19 38.74778272969625 40.0 38.0 41.0 34.5 41.0 20-21 38.84192375820439 40.0 38.0 41.0 35.0 41.0 22-23 38.77470634591014 40.0 38.0 41.0 35.0 41.0 24-25 38.69160152852766 40.0 38.0 41.0 34.0 41.0 26-27 38.520945816427584 40.0 38.0 41.0 34.0 41.0 28-29 38.36314778725564 40.0 38.0 41.0 34.0 41.0 30-31 38.30034228583396 40.0 38.0 41.0 34.0 41.0 32-33 38.192574607130055 40.0 38.0 41.0 34.0 41.0 34-35 38.06699377726662 40.0 37.0 41.0 34.0 41.0 36-37 37.8878093174162 40.0 37.0 41.0 33.0 41.0 38-39 37.74169754959905 40.0 37.0 41.0 33.0 41.0 40-41 37.50546397168709 40.0 37.0 41.0 33.0 41.0 42-43 37.266556979010254 40.0 37.0 41.0 32.5 41.0 44-45 36.98535545545226 40.0 36.0 41.0 31.5 41.0 46-47 36.57438881966853 40.0 36.0 41.0 30.5 41.0 48-49 36.16559641332013 39.0 36.0 41.0 30.0 41.0 50-51 35.911965893883405 39.0 35.0 41.0 29.0 41.0 52-53 35.683692658026544 39.0 35.0 40.0 28.0 41.0 54-55 35.41929349080079 39.0 35.0 40.0 28.0 41.0 56-57 35.17896925550835 38.0 35.0 40.0 27.0 41.0 58-59 34.85265424604823 38.0 34.0 40.0 26.0 41.0 60-61 34.528544082723656 38.0 34.0 40.0 26.0 41.0 62-63 34.200051737786154 37.5 34.0 40.0 26.0 41.0 64-65 33.79500344326948 37.0 33.5 39.5 24.5 41.0 66-67 33.37811669133751 36.0 33.0 39.0 23.0 40.0 68-69 32.94604849330337 36.0 32.5 39.0 22.0 40.0 70-71 32.55792191587773 35.0 32.0 38.0 22.0 40.0 72-73 32.17663794908931 35.0 32.0 37.5 20.5 40.0 74-75 31.704353606026075 35.0 31.0 37.0 19.5 39.0 76-77 30.244018650451352 33.5 29.5 35.5 18.0 38.0 78-79 30.935207962826887 34.0 31.0 36.0 18.0 38.5 80-81 30.60165099446592 34.0 31.0 36.0 11.5 37.5 82-83 30.433261805622116 34.0 31.0 35.0 12.5 37.0 84-85 30.11329732099436 34.0 30.5 35.0 8.5 36.5 86-87 29.75739770472367 34.0 30.0 35.0 7.0 36.0 88-89 29.392452406561382 34.0 30.0 35.0 2.0 36.0 90-91 28.96569775903503 34.0 29.0 35.0 2.0 35.0 92-93 28.559439882999833 34.0 29.0 35.0 2.0 35.0 94-95 28.10253355412855 34.0 29.0 35.0 2.0 35.0 96-97 27.507454500921163 33.0 27.0 35.0 2.0 35.0 98-99 26.765961062656856 33.0 26.0 35.0 2.0 35.0 100-101 23.625505841133936 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2565440378708388 1101 2 0.20352054709002232 1101 3 0.2140768095822736 1101 4 0.16312357159380042 1101 5 0.1965326742011797 1101 6 0.24552615824583768 1101 7 0.1419350006725253 1101 8 0.2349912769667455 1101 9 0.2658442441030857 1101 10-11 0.22712078513830392 1101 12-13 0.22473170052565195 1101 14-15 0.22782012707003219 1101 16-17 0.35222915601482185 1101 18-19 0.2358224200054977 1101 20-21 0.24337346299075335 1101 22-23 0.33683009282656684 1101 24-25 0.2875014801214135 1101 26-27 0.28084958397960946 1101 28-29 0.3530463060254192 1101 30-31 0.2703987571391835 1101 32-33 0.3255876513458489 1101 34-35 0.43515630295709684 1101 36-37 0.4651821604678972 1101 38-39 0.43407131251974107 1101 40-41 0.5280687102121462 1101 42-43 0.49709677598900726 1101 44-45 0.6813742503108742 1101 46-47 0.6452878178350687 1101 48-49 0.7498613736063362 1101 50-51 0.4784500160266063 1101 52-53 0.6234684479804073 1101 54-55 0.7061486876786134 1101 56-57 0.7198985939467093 1101 58-59 0.7559817129276851 1101 60-61 0.6330002459023589 1101 62-63 0.6883955023352257 1101 64-65 0.6249214422348004 1101 66-67 0.6464957326513527 1101 68-69 0.8128454391089477 1101 70-71 0.7783819446980615 1101 72-73 0.810832838554731 1101 74-75 0.6944882674329875 1101 76-77 0.5495395344747074 1101 78-79 0.7513173003830609 1101 80-81 0.9952799952863209 1101 82-83 0.7769891856719191 1101 84-85 0.7538040349716084 1101 86-87 0.5580470243053277 1101 88-89 0.3871581898956791 1101 90-91 0.34353549056526234 1101 92-93 0.5383696907267108 1101 94-95 0.5898716976943348 1101 96-97 0.43769881114706877 1101 98-99 0.6149880646619827 1101 100-101 0.8530733716746894 1102 1 0.23294253150362465 1102 2 0.22806558769980256 1102 3 0.17514533745516303 1102 4 0.1737457288681341 1102 5 0.13998061790473315 1102 6 0.20911379104335026 1102 7 0.20352526681821814 1102 8 0.21951665787349128 1102 9 0.2958170722894451 1102 10-11 0.29522620454256554 1102 12-13 0.1607583529023202 1102 14-15 0.249147041959624 1102 16-17 0.3749627423819035 1102 18-19 0.3562701549479854 1102 20-21 0.2642335421227031 1102 22-23 0.304972250889918 1102 24-25 0.2854422248559487 1102 26-27 0.22674594972898632 1102 28-29 0.22437346748318987 1102 30-31 0.42933578526933047 1102 32-33 0.4139770022304532 1102 34-35 0.4122418999233233 1102 36-37 0.5707647106732807 1102 38-39 0.4934999976044949 1102 40-41 0.6154578161723521 1102 42-43 0.5386965267907655 1102 44-45 0.44251009129740737 1102 46-47 0.5729973144907845 1102 48-49 0.8373339018014079 1102 50-51 0.7431467125061104 1102 52-53 0.551542043651331 1102 54-55 0.5703759183398631 1102 56-57 0.7637914288397241 1102 58-59 0.7696309055812662 1102 60-61 0.6684209980668143 1102 62-63 0.6757560063863437 1102 64-65 0.7614404912706405 1102 66-67 0.743662088916075 1102 68-69 0.7105303282601128 1102 70-71 0.652171089451663 1102 72-73 0.6495394404999857 1102 74-75 0.6551939291315847 1102 76-77 0.7035719105207718 1102 78-79 0.8611354810057605 1102 80-81 0.9461981330061597 1102 82-83 0.7161562947914888 1102 84-85 0.6498665054861661 1102 86-87 0.7387424616433123 1102 88-89 0.7262539586212995 1102 90-91 0.8503867984450864 1102 92-93 0.9067446091537406 1102 94-95 0.9301386652837671 1102 96-97 0.8873578073515596 1102 98-99 1.0434220608253852 1102 100-101 1.0728153462913141 1103 1 0.20112665000313257 1103 2 0.2165506030629416 1103 3 0.15896204752932164 1103 4 0.18606655191797472 1103 5 0.19053245301856947 1103 6 0.20111603425498004 1103 7 0.2256731924789932 1103 8 0.22332818094177753 1103 9 0.14619174083892972 1103 10-11 0.11852655491807695 1103 12-13 0.15759120584817765 1103 14-15 0.2828817367135912 1103 16-17 0.3126580445207878 1103 18-19 0.3298621059947777 1103 20-21 0.3107702894489961 1103 22-23 0.23974207329391817 1103 24-25 0.21151518144088044 1103 26-27 0.25386594892062675 1103 28-29 0.28476903562453515 1103 30-31 0.29134037779044064 1103 32-33 0.37308554453864673 1103 34-35 0.3329478632117855 1103 36-37 0.4853843625207759 1103 38-39 0.3907122345597287 1103 40-41 0.4698261003259674 1103 42-43 0.3797724768506754 1103 44-45 0.5367531884114669 1103 46-47 0.620106863886484 1103 48-49 0.6291280332431697 1103 50-51 0.7316355170780824 1103 52-53 0.5646274448401272 1103 54-55 0.4784703420559282 1103 56-57 0.613945481230175 1103 58-59 0.6192567843619514 1103 60-61 0.607360423774324 1103 62-63 0.60661473373424 1103 64-65 0.5779036353203608 1103 66-67 0.4391381103815135 1103 68-69 0.6018880270483251 1103 70-71 0.7053503198717763 1103 72-73 0.445273928110538 1103 74-75 0.2743643365894144 1103 76-77 0.3544946249752563 1103 78-79 0.6527990952541991 1103 80-81 0.8024235505138186 1103 82-83 0.6343754845239111 1103 84-85 0.7197018348905075 1103 86-87 0.4285184604577097 1103 88-89 0.2365911308442108 1103 90-91 0.3007207961530334 1103 92-93 0.24927287875679127 1103 94-95 0.41925332084889533 1103 96-97 0.636642278415902 1103 98-99 0.9240944012007475 1103 100-101 0.5748893065955656 1104 1 0.17351121399228475 1104 2 0.12848997891656921 1104 3 0.14189424643095094 1104 4 0.11557199583660349 1104 5 -0.03946249163836768 1104 6 0.13711620867113083 1104 7 0.010067580094357709 1104 8 0.16970838725246296 1104 9 0.20919049040232096 1104 10-11 0.20748355997478285 1104 12-13 0.18240530957400836 1104 14-15 0.23616818662277694 1104 16-17 0.22178041788853164 1104 18-19 0.1135304409700737 1104 20-21 0.2551467539882424 1104 22-23 0.22285890804052144 1104 24-25 0.2817365309767439 1104 26-27 0.2577869057022113 1104 28-29 0.25588340492620887 1104 30-31 0.3013025376619183 1104 32-33 0.2749081024513984 1104 34-35 0.268330995080845 1104 36-37 0.2964569264517891 1104 38-39 0.3146390869705087 1104 40-41 0.3760715156566832 1104 42-43 0.24535667329413258 1104 44-45 0.35593315090291355 1104 46-47 0.21366432197097396 1104 48-49 0.04352354275407322 1104 50-51 0.1970920354501402 1104 52-53 0.14629371861624918 1104 54-55 0.19291681378530257 1104 56-57 -0.04559873848693741 1104 58-59 0.08298912643017786 1104 60-61 0.22856602089754574 1104 62-63 0.12333938014410961 1104 64-65 0.1775835998557369 1104 66-67 -0.058763899537495945 1104 68-69 -0.0060905579189167725 1104 70-71 0.17603841428904587 1104 72-73 0.2280999330132687 1104 74-75 0.09506567119089837 1104 76-77 0.2429600399335321 1104 78-79 0.5300482800630952 1104 80-81 0.7081012049436488 1104 82-83 0.713658979351159 1104 84-85 0.5760158584484998 1104 86-87 0.624125262869196 1104 88-89 0.5299272984399579 1104 90-91 0.5472414438001643 1104 92-93 0.6376787923726503 1104 94-95 0.7550293886822423 1104 96-97 0.5742305936240335 1104 98-99 0.9424042360459168 1104 100-101 0.9037311969940944 1105 1 0.15931744517206425 1105 2 0.12180526129970559 1105 3 0.016670153249350506 1105 4 0.057001602276486096 1105 5 0.09364679996989622 1105 6 0.1475798937407049 1105 7 0.11866658348431258 1105 8 0.04248829533052145 1105 9 0.015095435786356859 1105 10-11 0.1728962824985416 1105 12-13 0.182569713182545 1105 14-15 0.25096811334868363 1105 16-17 0.19619391637673544 1105 18-19 0.19294561694110968 1105 20-21 0.21877496410377262 1105 22-23 0.02242286541805072 1105 24-25 0.10798172750980939 1105 26-27 0.23870167928318864 1105 28-29 0.124513601263736 1105 30-31 0.12653043862662372 1105 32-33 0.10315855989993139 1105 34-35 0.06531492211959744 1105 36-37 0.16490856863543968 1105 38-39 0.3214125670990242 1105 40-41 0.3685128089033256 1105 42-43 0.37511871772375116 1105 44-45 0.42679059161984867 1105 46-47 0.6058209577019724 1105 48-49 0.5469469455948825 1105 50-51 0.3965812779482931 1105 52-53 0.39071969016696784 1105 54-55 0.46057695164762436 1105 56-57 0.377028395158014 1105 58-59 0.5175770728412914 1105 60-61 0.44220272134027994 1105 62-63 0.47304901095380814 1105 64-65 0.3379059338415402 1105 66-67 0.3758466877461544 1105 68-69 0.45402445771357947 1105 70-71 0.42167773078168835 1105 72-73 0.3814951891329912 1105 74-75 0.3640171106358281 1105 76-77 0.17425399667299857 1105 78-79 0.29403725189990837 1105 80-81 0.5821670116688118 1105 82-83 0.3579551775370078 1105 84-85 0.2669931620029118 1105 86-87 0.19694319732181498 1105 88-89 0.239188907547355 1105 90-91 0.23682787738934152 1105 92-93 0.5694471783048485 1105 94-95 0.39105240621656634 1105 96-97 0.7542116370389387 1105 98-99 0.9626662732716298 1105 100-101 1.0670907525743445 1106 1 0.03930356336572771 1106 2 -0.018337239691852858 1106 3 0.04354136408282727 1106 4 0.03360084302666877 1106 5 0.05489814565520135 1106 6 0.037467422779180026 1106 7 0.05757955305765705 1106 8 -0.006983346122254375 1106 9 -0.012080324879967463 1106 10-11 -0.025525016158603364 1106 12-13 -0.09947176225514909 1106 14-15 -0.012318758428754961 1106 16-17 0.013885428643725106 1106 18-19 0.07543895934011147 1106 20-21 0.01523297872216034 1106 22-23 0.11346201777795528 1106 24-25 0.15360400072642477 1106 26-27 0.07239268160626722 1106 28-29 0.20408255701193667 1106 30-31 0.1966193955080371 1106 32-33 0.1454661977264422 1106 34-35 0.09268191175074492 1106 36-37 0.10775235581885312 1106 38-39 0.11280655542697815 1106 40-41 0.34388676776784877 1106 42-43 0.32172688249229964 1106 44-45 0.1398586124708885 1106 46-47 0.3056977212579781 1106 48-49 0.45755448709864766 1106 50-51 0.08802626066685804 1106 52-53 0.3446087207069368 1106 54-55 0.3794285863011453 1106 56-57 0.23469738490247494 1106 58-59 0.3554715711131422 1106 60-61 0.26908738902682927 1106 62-63 0.22074363954466492 1106 64-65 0.1376856664042947 1106 66-67 0.24626781642199802 1106 68-69 0.335533325071502 1106 70-71 0.40427235370650294 1106 72-73 0.3587318597658964 1106 74-75 0.5527459337986116 1106 76-77 0.4917153259267799 1106 78-79 0.43292259951167367 1106 80-81 0.6753628017985633 1106 82-83 0.6388705268485815 1106 84-85 0.4545498224369311 1106 86-87 0.41028743778869625 1106 88-89 0.25045371008574335 1106 90-91 0.33963823491668066 1106 92-93 0.4896763697604527 1106 94-95 0.6891535734215068 1106 96-97 0.7202366242903402 1106 98-99 0.7304895210477049 1106 100-101 0.8206036994835202 1107 1 0.12854507279968885 1107 2 -0.0010403843459414475 1107 3 0.02100048357968376 1107 4 0.010297698372582431 1107 5 0.0569849381080374 1107 6 0.05743094479176136 1107 7 0.028244961862689877 1107 8 0.044753760795984476 1107 9 0.04948822857915047 1107 10-11 0.004361147363404427 1107 12-13 0.15431565912849976 1107 14-15 0.117583128363691 1107 16-17 0.026467189650013268 1107 18-19 0.16082009141558018 1107 20-21 0.158665054193861 1107 22-23 0.03559283538801594 1107 24-25 0.12885620198428427 1107 26-27 0.12025243003394337 1107 28-29 0.0920517394018745 1107 30-31 0.138244552740737 1107 32-33 0.15150330464468453 1107 34-35 0.14966807527275705 1107 36-37 0.20828694701381778 1107 38-39 0.014926681213140114 1107 40-41 0.14998488097540275 1107 42-43 0.11002562463066567 1107 44-45 0.042681882911139724 1107 46-47 0.12410023698124917 1107 48-49 0.2246670657150034 1107 50-51 0.07561431098132942 1107 52-53 0.12640557605285352 1107 54-55 -0.04547770300702325 1107 56-57 0.13104581257543657 1107 58-59 0.16394704281125883 1107 60-61 0.22298109971865898 1107 62-63 0.2741895514943451 1107 64-65 0.17084227017787867 1107 66-67 0.14231875981823094 1107 68-69 0.30470313639225566 1107 70-71 0.1470717247756852 1107 72-73 0.11041110504891094 1107 74-75 0.40429939291811223 1107 76-77 0.14547381649281732 1107 78-79 0.5086678825305384 1107 80-81 0.6119495942513957 1107 82-83 0.4720831054649324 1107 84-85 0.3616586274683762 1107 86-87 0.1723119660905823 1107 88-89 0.13824176040020575 1107 90-91 -0.053551702190237194 1107 92-93 -0.0716116805540139 1107 94-95 -0.11989233852817449 1107 96-97 -0.05258475935746176 1107 98-99 0.03901342041877598 1107 100-101 0.127282944766538 1108 1 0.09221718164322823 1108 2 0.08330247279691605 1108 3 0.0799936808585926 1108 4 0.09357388884877338 1108 5 0.06256725103320804 1108 6 0.13145407404346088 1108 7 0.09568441764500335 1108 8 0.09979049548986296 1108 9 0.08254809252473194 1108 10-11 0.14702985484640152 1108 12-13 0.07579457069311957 1108 14-15 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-0.6868140635462723 2316 76-77 -0.6463175643339163 2316 78-79 -0.35435586417130693 2316 80-81 -0.5966840029341824 2316 82-83 -0.6909198848604845 2316 84-85 -0.849995198389145 2316 86-87 -0.3536591860642133 2316 88-89 -0.7187541938654043 2316 90-91 -1.0661466010468779 2316 92-93 -1.016434547748382 2316 94-95 -1.108419691210674 2316 96-97 -0.991569104124391 2316 98-99 -1.0874161310324126 2316 100-101 -1.6674980578719811 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 10.0 5 23.0 6 59.0 7 114.0 8 286.0 9 581.0 10 1024.0 11 1714.0 12 2668.0 13 3792.0 14 4899.0 15 6947.0 16 9293.0 17 13349.0 18 11867.0 19 6707.0 20 5526.0 21 5499.0 22 5634.0 23 6164.0 24 7038.0 25 8315.0 26 10303.0 27 12460.0 28 15970.0 29 19758.0 30 24714.0 31 31261.0 32 40462.0 33 53074.0 34 73028.0 35 106260.0 36 165307.0 37 256328.0 38 200290.0 39 16110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16131655242082 19.529470456752577 15.247692446991525 22.061520543835076 2 15.402242743477895 21.1031777376425 40.077746073867644 23.41683344501196 3 19.319586732189787 25.510172384259892 30.387928930433016 24.782311953117304 4 12.88253126453184 17.51266377716012 37.53518701922906 32.069617939078974 5 13.373495472884345 37.74829911445677 33.97058484192814 14.907620570730746 6 30.742894263229076 36.88744413561512 17.709764331278024 14.659897269877781 7 27.53559524189855 33.69611904482995 21.085765807979158 17.68251990529234 8 26.327788604552925 34.50040644778832 19.953835340723938 19.217969606934815 9 26.478919736323654 16.862702292081543 20.65837442183246 36.00000354976235 10-11 24.481468465686223 27.177956685799888 27.357929636610827 20.982645211903066 12-13 25.936783945106377 24.660020908137312 28.16151269840988 21.24168244834643 14-15 22.796130049093215 25.489911575420027 27.191002062411922 24.52295631307484 16-17 21.397400064161985 29.064313291514154 27.590451312547394 21.947835331776467 18-19 21.132156684379265 27.018892204608235 30.916166535393764 20.93278457561874 20-21 22.0578956196237 25.96392860977919 31.163645334973346 20.81453043562376 22-23 21.76412539180502 26.064839958964747 31.026032182145407 21.14500246708483 24-25 21.586005711881885 26.198110712529754 31.185934357732787 21.029949217855577 26-27 21.523666932324662 26.399275090924256 30.92809306668134 21.14896491006974 28-29 21.144794442435227 26.432512907670496 31.210713976671894 21.211978673222383 30-31 21.40837568521272 26.327526132404184 31.09891475620853 21.165183426174572 32-33 21.29394908457757 26.409896084545174 30.929568315348504 21.366586515528745 34-35 21.66730047555635 26.3498687194679 30.84074120584619 21.14208959912956 36-37 21.699138545846218 26.186280196495517 30.770968404767622 21.343612852890647 38-39 21.515892854527873 26.411927772701716 30.83731640201873 21.23486297075168 40-41 21.659837911550277 26.249533495365572 30.82130110084 21.26932749224415 42-43 21.53475583671919 26.510278270346642 30.513931267561635 21.44103462537253 44-45 21.67852244373812 26.545462774664507 30.173326121210696 21.602688660386676 46-47 21.866882497388644 26.774945887013686 29.60710288966453 21.751068725933145 48-49 21.995426919231832 27.048224126218095 29.223832025143516 21.732516929406557 50-51 21.95269575280598 27.172785370135188 29.00178471429041 21.872734162768428 52-53 22.11368946061737 27.363212999673852 28.80513135678771 21.717966182921067 54-55 21.84783674614032 27.330174266368896 28.823594405265485 21.998394582225302 56-57 21.979451158229633 27.552620077163542 28.616403131801448 21.851525632805377 58-59 22.13857994493365 27.226648385171575 28.667736045384256 21.967035624510515 60-61 22.01447238107409 27.214297377790558 29.13294392440426 21.638286316731094 62-63 22.030855435420698 27.236672966018556 29.014882828948856 21.71758876961189 64-65 21.864908676923676 27.198785169446587 29.010380819380487 21.92592533424925 66-67 21.84281335361449 27.463601301354423 28.72879220039296 21.964793144638126 68-69 21.816676376870696 27.396131507962885 28.845606868523543 21.94158524664288 70-71 21.863312915232637 27.391065498261412 28.805851729444488 21.93976985706146 72-73 21.896517446494283 27.389673578284228 28.685002778019385 22.0288061972021 74-75 21.823231632962777 27.642410989248955 28.4041875300637 22.130169847724567 76-77 21.94130685108394 27.487092787713795 28.428304235251833 22.143296125950428 78-79 21.754185570963 27.550526835225536 28.4562023842489 22.239085209562557 80-81 21.97863392357164 27.69980112403524 28.168237884407006 22.15332706798612 82-83 21.766362878769726 27.635069352922017 28.42897371520262 22.16959405310564 84-85 21.81771389143414 27.47399224814801 28.22539386019599 22.48290000022186 86-87 21.902787983344517 27.280589189553762 28.260856060686734 22.55576676641499 88-89 21.84233343184055 27.29373165746822 28.322599596564142 22.54133531412709 90-91 21.699743261843636 27.355969716789325 28.536228655812018 22.40805836555502 92-93 21.716419632350462 27.12471402176363 28.447402190562233 22.711464155323675 94-95 21.41046746389191 27.23834668204912 28.477980165509287 22.873205688549685 96-97 21.556984537180345 27.197221946128614 28.454064137333695 22.79172937935735 98-99 21.440631149705037 27.200005324660054 28.21013770902064 23.149225816614273 100-101 21.059394891387065 27.333497511808645 28.4130375458424 23.194070050961887 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 415.0 1 305.0 2 233.0 3 283.5 4 248.5 5 223.5 6 380.5 7 826.5 8 1423.5 9 1787.5 10 1677.5 11 1477.5 12 1544.0 13 1714.5 14 1852.5 15 1998.0 16 2165.5 17 2250.5 18 2408.0 19 2605.0 20 2757.5 21 3062.0 22 3536.5 23 4143.0 24 5031.5 25 6152.5 26 7643.5 27 9488.0 28 10989.5 29 12700.0 30 14915.5 31 16870.0 32 18690.5 33 20701.0 34 23519.5 35 26034.5 36 28406.5 37 31005.0 38 32663.0 39 33559.5 40 34540.0 41 36205.5 42 37894.0 43 39264.5 44 40545.5 45 41666.5 46 42001.0 47 42413.5 48 44628.5 49 44361.0 50 41207.5 51 39029.5 52 37129.0 53 35769.0 54 34094.0 55 31189.0 56 27931.5 57 24767.0 58 22309.5 59 20007.0 60 17230.5 61 13928.5 62 10717.0 63 8267.0 64 6085.0 65 4399.5 66 3340.5 67 2662.5 68 1883.5 69 1172.5 70 763.5 71 524.5 72 396.5 73 318.5 74 248.5 75 193.0 76 126.0 77 60.5 78 30.5 79 19.0 80 13.0 81 4.5 82 1.5 83 4.5 84 5.0 85 2.5 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028220610630118315 2 0.008075709331260271 3 0.009673102385795272 4 0.0 5 0.00621208410096944 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.7748811717055544E-4 14-15 0.0 16-17 4.437202929263886E-5 18-19 0.004304086841385969 20-21 0.0013311608787791658 22-23 0.0 24-25 0.005502131632287219 26-27 0.0065670603353105515 28-29 0.0074101288918706895 30-31 0.032036605149285254 32-33 5.768363808043051E-4 34-35 0.004481574958556524 36-37 0.017260719394836515 38-39 0.007765105126211799 40-41 0.0093624981807468 42-43 0.007986965272674994 44-45 0.0026179497282656926 46-47 0.0023960895818024982 48-49 0.002174229435339304 50-51 0.003993482636337497 52-53 0.003416646255533192 54-55 0.0023517175525098596 56-57 7.543244979748606E-4 58-59 0.0011980447909012491 60-61 0.0 62-63 4.437202929263886E-5 64-65 0.008386313536308745 66-67 8.874405858527772E-4 68-69 0.0012867888494865268 70-71 0.005147155397946107 72-73 0.011891703850427215 74-75 0.005502131632287219 76-77 0.0038603665484595803 78-79 0.009584358327209993 80-81 2.6623217575583315E-4 82-83 0.005590875690872496 84-85 7.543244979748606E-4 86-87 0.0 88-89 0.0013311608787791658 90-91 6.21208410096944E-4 92-93 1.7748811717055544E-4 94-95 9.761846444380548E-4 96-97 3.5497623434111087E-4 98-99 3.10604205048472E-4 100-101 0.0011980447909012491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.38884964373264 #Duplication Level Percentage of deduplicated Percentage of total 1 83.04992198457043 54.30538861572801 2 10.429373946739236 13.639295297631888 3 2.818381872648664 5.528722455277354 4 1.173114486815615 3.068344271730833 5 0.6752731902233298 2.207766855197849 6 0.3835775829436363 1.5049018138684689 7 0.2641094747757534 1.208887031491786 8 0.1840855146153138 0.9629712029415921 9 0.14141232493729233 0.8322110327785746 >10 0.7998233359550933 9.570376089708756 >50 0.05347281389668773 2.3564028114599846 >100 0.026230299322923385 3.006609582206109 >500 6.795403089266811E-4 0.27344904582301266 >1k 2.7181612357067244E-4 0.4431882975363549 >5k 2.7181612357067244E-4 1.091485596619513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6893 0.6117127958283193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5389 0.4782417317160616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.31166913375149535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 0.13089748641328464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.6623217575583315E-4 0.0 0.0 0.0 0.0 7 2.6623217575583315E-4 0.0 0.0 0.0 0.0 8 2.6623217575583315E-4 0.0 0.0 0.0 0.0 9 2.6623217575583315E-4 0.0 0.0 0.0 0.0 10-11 2.6623217575583315E-4 0.0 0.0 0.0 0.0 12-13 2.6623217575583315E-4 0.0 0.0 0.0 0.0 14-15 2.6623217575583315E-4 0.0 0.0 1.3311608787791658E-4 0.0 16-17 2.6623217575583315E-4 0.0 0.0 2.218601464631943E-4 0.0 18-19 2.6623217575583315E-4 0.0 0.0 2.6623217575583315E-4 0.0 20-21 2.6623217575583315E-4 0.0 0.0 3.5497623434111087E-4 0.0 22-23 3.5497623434111087E-4 0.0 0.0 4.437202929263886E-4 0.0 24-25 3.5497623434111087E-4 0.0 0.0 5.324643515116663E-4 0.0 26-27 3.5497623434111087E-4 0.0 0.0 9.761846444380548E-4 0.0 28-29 4.437202929263886E-4 0.0 0.0 0.002262973493924582 0.0 30-31 4.437202929263886E-4 0.0 0.0 0.005235899456531385 0.0 32-33 4.437202929263886E-4 0.0 0.0 0.007942593243382355 0.0 34-35 4.880923222190275E-4 0.0 0.0 0.010383054854477493 0.0 36-37 5.324643515116663E-4 0.0 0.0 0.014820257783741377 0.0 38-39 5.324643515116663E-4 0.0 0.0 0.019656808976639014 0.0 40-41 5.768363808043051E-4 0.0 0.0 0.026223869311949567 0.0 42-43 6.21208410096944E-4 0.0 0.0 0.03429957864320984 0.0 44-45 6.21208410096944E-4 0.0 0.0 0.04117724318356886 0.0 46-47 7.099524686822217E-4 0.0 0.0 0.05666308140669982 0.0 48-49 7.986965272674994E-4 0.0 0.0 0.07631989038333883 0.0 50-51 7.986965272674994E-4 0.0 0.0 0.08435122768530648 0.0 52-53 7.986965272674994E-4 0.0 0.0 0.09779595256097603 4.437202929263886E-5 54-55 7.986965272674994E-4 0.0 0.0 0.11687592515681075 8.874405858527772E-5 56-57 7.986965272674994E-4 0.0 0.0 0.1525510367080924 8.874405858527772E-5 58-59 7.986965272674994E-4 0.0 0.0 0.20309077807240805 8.874405858527772E-5 60-61 7.986965272674994E-4 0.0 0.0 0.26325924979322635 8.874405858527772E-5 62-63 7.986965272674994E-4 0.0 0.0 0.3357187736281056 8.874405858527772E-5 64-65 7.986965272674994E-4 0.0 0.0 0.41279298850941926 8.874405858527772E-5 66-67 7.986965272674994E-4 0.0 0.0 0.5168453972006575 8.874405858527772E-5 68-69 7.986965272674994E-4 0.0 0.0 0.651558878133109 8.874405858527772E-5 70-71 7.986965272674994E-4 0.0 0.0 0.7143896716114856 8.874405858527772E-5 72-73 8.874405858527772E-4 0.0 0.0 0.7899996095261422 8.874405858527772E-5 74-75 8.874405858527772E-4 0.0 0.0 0.881450361898271 8.874405858527772E-5 76-77 8.874405858527772E-4 0.0 0.0 0.9866120713218249 8.874405858527772E-5 78-79 8.874405858527772E-4 0.0 0.0 1.086493509259555 8.874405858527772E-5 80-81 0.0011536727616086103 0.0 0.0 1.2522674106968539 8.874405858527772E-5 82-83 0.0011536727616086103 0.0 0.0 1.4383637015501813 8.874405858527772E-5 84-85 0.0011536727616086103 0.0 0.0 1.7164432091271489 8.874405858527772E-5 86-87 0.0011536727616086103 0.0 0.0 2.132564099833516 8.874405858527772E-5 88-89 0.0011536727616086103 0.0 0.0 2.5569381879883144 8.874405858527772E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4025 0.0 38.01211 1 GTATCAA 6645 0.0 37.468674 1 TATCAAC 8340 0.0 29.790052 2 ATCAACG 8370 0.0 29.568453 3 TCAACGC 8445 0.0 29.18947 4 CAACGCA 8600 0.0 28.663382 5 AACGCAG 8875 0.0 27.772755 6 TAACACG 95 0.0012271178 24.997978 5 TAGACCG 115 1.2824347E-4 24.780607 5 ACGCAGA 9945 0.0 24.73688 7 CGCAGAG 9975 0.0 24.424429 8 GCAGAGT 10405 0.0 23.049911 9 GAGTACT 5930 0.0 22.54616 12-13 GTACATG 5350 0.0 21.670456 1 AGTACTT 6055 0.0 21.139442 12-13 GTACTTT 6315 0.0 20.90977 14-15 TACATGG 5420 0.0 20.684658 2 ACATGGG 5365 0.0 20.275991 3 AGAGTAC 9310 0.0 19.971104 10-11 CAGAGTA 10215 0.0 18.782913 10-11 >>END_MODULE