FastQCFastQC Report
Thu 26 May 2016
SRR936861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195648
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54220.4534779466866502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.33747390536345145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.22121895407343967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.040.7717481
GTATCAA61650.039.159881
TATCAAC75600.031.877762
ATCAACG75650.031.8566933
TCAACGC76950.031.3185044
CAACGCA77450.031.0549455
AACGCAG80350.030.0461336
ACGCAGA91700.026.2743027
CGCAGAG91600.026.1992288
GTACATG59500.026.0382251
GCAGAGT96500.024.426729
TACATGG61800.024.3821742
ACATGGG61750.023.709123
GAGTACT49650.023.11433212-13
CATGGGG26350.021.647234
GTACTTT52650.021.3884314-15
AGTACTT51150.020.76420812-13
AGAGTAC85800.020.21613710-11
CATGGGA38150.019.6863064
CAGAGTA94900.019.60460910-11
Produced by FastQC (version 0.11.5)