##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936861_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1195648 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85723306524997 33.0 31.0 34.0 30.0 34.0 2 31.967438577240124 33.0 31.0 34.0 30.0 34.0 3 31.9776489401563 33.0 31.0 34.0 30.0 34.0 4 35.54985330130607 37.0 35.0 37.0 33.0 37.0 5 35.542603675998286 37.0 35.0 37.0 33.0 37.0 6 35.643228609088965 37.0 35.0 37.0 33.0 37.0 7 35.58492466010063 37.0 35.0 37.0 33.0 37.0 8 35.55087617760411 37.0 35.0 37.0 33.0 37.0 9 37.166160943689114 39.0 37.0 39.0 34.0 39.0 10-11 37.05249663780644 39.0 37.0 39.0 33.0 39.0 12-13 36.95728466906648 39.0 37.0 39.0 33.0 39.0 14-15 38.13946077775399 40.0 38.0 41.0 33.0 41.0 16-17 38.06730576223103 40.0 38.0 41.0 33.0 41.0 18-19 38.02352866395461 40.0 38.0 41.0 33.0 41.0 20-21 37.92459068220747 40.0 38.0 41.0 33.0 41.0 22-23 37.793973644417086 40.0 37.5 41.0 32.5 41.0 24-25 37.90820584319131 40.0 38.0 41.0 33.0 41.0 26-27 37.86191295431431 40.0 38.0 41.0 33.0 41.0 28-29 37.76501152513114 40.0 37.5 41.0 33.0 41.0 30-31 37.57644599413874 40.0 37.0 41.0 32.0 41.0 32-33 37.37908815972594 40.0 37.0 41.0 32.0 41.0 34-35 37.19228318033401 40.0 36.0 41.0 31.0 41.0 36-37 36.99966336246119 39.5 36.0 41.0 31.0 41.0 38-39 36.85124008069265 39.0 36.0 41.0 30.5 41.0 40-41 36.65330138970667 39.0 35.5 41.0 30.0 41.0 42-43 36.419328263836846 39.0 35.0 41.0 30.0 41.0 44-45 36.14411892128787 39.0 35.0 40.5 30.0 41.0 46-47 35.7743165212504 39.0 35.0 40.0 28.5 41.0 48-49 35.51462136013275 39.0 35.0 40.0 27.0 41.0 50-51 34.8026496928862 38.0 34.0 39.5 26.0 40.5 52-53 34.9904884213414 38.5 34.5 40.0 26.5 40.5 54-55 35.40026496092496 39.0 35.0 40.5 27.0 41.0 56-57 35.31653588681618 39.0 35.0 41.0 27.0 41.0 58-59 35.07654217629269 38.5 35.0 40.5 26.0 41.0 60-61 34.810599357001394 38.0 34.0 40.0 26.0 41.0 62-63 34.47532049566428 38.0 34.0 40.0 25.5 41.0 64-65 34.13455256061985 37.0 34.0 40.0 24.5 41.0 66-67 33.76914443046783 37.0 34.0 39.5 23.5 41.0 68-69 33.37723393507119 36.0 33.0 39.0 22.5 41.0 70-71 32.98417970840916 36.0 33.0 39.0 22.0 40.5 72-73 32.577069923589555 35.0 32.5 38.5 21.5 40.0 74-75 32.137192551653996 35.0 32.0 37.5 20.0 39.5 76-77 31.69630861256825 35.0 32.0 37.0 18.5 39.0 78-79 31.270091197409272 35.0 31.0 36.5 17.5 39.0 80-81 30.836458974547696 35.0 31.0 36.0 12.5 38.0 82-83 30.425112993121722 34.5 31.0 35.5 8.5 37.0 84-85 30.04201111029333 34.0 30.0 35.0 7.0 37.0 86-87 29.690251645969383 34.0 30.0 35.0 2.0 36.0 88-89 29.345542751712877 34.0 29.5 35.0 2.0 36.0 90-91 28.969836021973023 34.0 29.0 35.0 2.0 36.0 92-93 28.612797411947327 34.0 29.0 35.0 2.0 35.0 94-95 28.22254041323199 34.0 29.0 35.0 2.0 35.0 96-97 27.72270308652714 34.0 28.0 35.0 2.0 35.0 98-99 27.10789714029547 34.0 27.0 35.0 2.0 35.0 100-101 24.623443940022483 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35705409457179016 1101 2 0.38142923184329547 1101 3 0.29000496197240366 1101 4 0.34299423315054867 1101 5 0.37526662124579246 1101 6 0.23657362541910487 1101 7 0.3378240042461371 1101 8 0.22613751058755582 1101 9 0.32821227423303156 1101 10-11 0.46581198919248834 1101 12-13 0.42548022695829246 1101 14-15 0.48942519348977953 1101 16-17 0.43565396905869846 1101 18-19 0.5555947226452673 1101 20-21 0.5903940650831316 1101 22-23 0.5595785791444783 1101 24-25 0.48998176086903555 1101 26-27 0.4747399143605975 1101 28-29 0.46845421039108004 1101 30-31 0.4681958008888998 1101 32-33 0.5594452159660008 1101 34-35 0.5796803353732471 1101 36-37 0.5464557789222084 1101 38-39 0.7004356772334361 1101 40-41 0.6498056322274195 1101 42-43 0.4521685110533866 1101 44-45 0.7519960868280506 1101 46-47 0.5443728624307411 1101 48-49 0.745495440713519 1101 50-51 0.8117055489860405 1101 52-53 0.8472149776879405 1101 54-55 0.8866881071570987 1101 56-57 0.8400915676303882 1101 58-59 0.9238345583000935 1101 60-61 0.7606693358344643 1101 62-63 0.8701997576360156 1101 64-65 0.9701411142960978 1101 66-67 0.9510549321978701 1101 68-69 0.8174915571894275 1101 70-71 0.757468895884152 1101 72-73 0.8420325583944077 1101 74-75 0.6842517027395445 1101 76-77 0.7491165732554137 1101 78-79 0.7987962367999941 1101 80-81 0.7593033860014877 1101 82-83 0.7374429494256454 1101 84-85 0.6917230363852305 1101 86-87 0.7633499512302571 1101 88-89 0.7401153967365168 1101 90-91 0.7670163999027615 1101 92-93 0.5687514561085791 1101 94-95 0.799702232275699 1101 96-97 0.8029340703649588 1101 98-99 0.6459849613990123 1101 100-101 0.7421237481715437 1102 1 0.21476160445321213 1102 2 0.36098703816741207 1102 3 0.16580782758505208 1102 4 0.19899308031787655 1102 5 0.17140051452642524 1102 6 0.1734972090818161 1102 7 0.20135743639369963 1102 8 0.2033073063714852 1102 9 0.2778787762093131 1102 10-11 0.20413955836244924 1102 12-13 0.27303869204392583 1102 14-15 0.2543708456636864 1102 16-17 0.4089741271614642 1102 18-19 0.3569575355569867 1102 20-21 0.3145067956496703 1102 22-23 0.3160735560878223 1102 24-25 0.15633963306929388 1102 26-27 0.28414167654768363 1102 28-29 0.057397309864064994 1102 30-31 0.18198450312868886 1102 32-33 0.3899479333573126 1102 34-35 0.12669943945757467 1102 36-37 0.4163092044821539 1102 38-39 0.21615331557335793 1102 40-41 0.2850473153631228 1102 42-43 0.29524945637618316 1102 44-45 0.20505932793476944 1102 46-47 0.19579537560597515 1102 48-49 0.14428290777544106 1102 50-51 0.2685875483272824 1102 52-53 0.31455481629136983 1102 54-55 0.4020969510306145 1102 56-57 0.554302288974263 1102 58-59 0.42556380731195276 1102 60-61 0.37915377259335514 1102 62-63 0.5716984401129608 1102 64-65 0.4830590161406292 1102 66-67 0.38472588081446446 1102 68-69 0.4595987706281619 1102 70-71 0.3936575487168241 1102 72-73 0.2701452066157515 1102 74-75 0.30992075741675507 1102 76-77 0.41841828603539 1102 78-79 0.28009358528484185 1102 80-81 0.28247326413060136 1102 82-83 0.4057565199131261 1102 84-85 0.3734485798634921 1102 86-87 0.45926974701418644 1102 88-89 0.4765696128102981 1102 90-91 0.24161290846666006 1102 92-93 0.20390050090436418 1102 94-95 0.7531154404443434 1102 96-97 0.5346431448050133 1102 98-99 0.4453303171302352 1102 100-101 0.6316370880924929 1103 1 0.12315559980373081 1103 2 0.22491081513466682 1103 3 0.21407800629001272 1103 4 0.32832399225748077 1103 5 0.27722457687222146 1103 6 0.34077176766072625 1103 7 0.21711429997665022 1103 8 0.25574116315316786 1103 9 0.22229609019184693 1103 10-11 0.4204880355804974 1103 12-13 0.42690274808190054 1103 14-15 0.5183040335099207 1103 16-17 0.4979056617451718 1103 18-19 0.533609907861532 1103 20-21 0.391295752873404 1103 22-23 0.4957107031162735 1103 24-25 0.26907306102011574 1103 26-27 0.39882213905423924 1103 28-29 0.425833221778241 1103 30-31 0.4480732909566001 1103 32-33 0.6034922229885566 1103 34-35 0.42281656166164794 1103 36-37 0.5786710298874596 1103 38-39 0.568538591871679 1103 40-41 0.6318064121222235 1103 42-43 0.4300145150542889 1103 44-45 0.5670526029715219 1103 46-47 0.47367828392366107 1103 48-49 0.5198011419893263 1103 50-51 0.5712019922460669 1103 52-53 0.6180111523563951 1103 54-55 0.4349790708692609 1103 56-57 0.5937860132405959 1103 58-59 0.5056465721773336 1103 60-61 0.5120887968327281 1103 62-63 0.620811782704564 1103 64-65 0.4730923866087835 1103 66-67 0.34779581127570225 1103 68-69 0.4507232943613886 1103 70-71 0.4995680894099692 1103 72-73 0.38882274920633364 1103 74-75 0.4419286829029332 1103 76-77 0.2776684170246462 1103 78-79 0.40610652651517753 1103 80-81 0.4282795729803901 1103 82-83 0.367136307379873 1103 84-85 0.26343794811372945 1103 86-87 0.29629875795624017 1103 88-89 0.32856700828595464 1103 90-91 0.3561954469622428 1103 92-93 0.4893744276826375 1103 94-95 0.5884670257959286 1103 96-97 0.33746233892855315 1103 98-99 0.33615776230550765 1103 100-101 0.3843802321399856 1104 1 0.22204189769992766 1104 2 0.23213458770497297 1104 3 0.27950354498287666 1104 4 0.2610953775041125 1104 5 0.13375196706298453 1104 6 0.11827473031220137 1104 7 0.1546310246220841 1104 8 0.24299475337705445 1104 9 0.16112275278088362 1104 10-11 0.2721700570644927 1104 12-13 0.22614074137929663 1104 14-15 0.42034157317122833 1104 16-17 0.3652062721150813 1104 18-19 0.271725534095971 1104 20-21 0.5619858913853264 1104 22-23 0.3556964314188349 1104 24-25 0.3437605490739415 1104 26-27 0.2281893421838177 1104 28-29 0.26484783752552943 1104 30-31 0.21567526350906974 1104 32-33 0.2361884269718857 1104 34-35 0.18989162824166783 1104 36-37 0.11565957430174478 1104 38-39 0.1537712077973623 1104 40-41 0.09800861149273743 1104 42-43 0.26285534740006966 1104 44-45 0.2758768444690318 1104 46-47 -0.03312816892239567 1104 48-49 0.054950931099504885 1104 50-51 0.14929366241694453 1104 52-53 0.04920436820308538 1104 54-55 0.033169623515263424 1104 56-57 0.14061374620693812 1104 58-59 0.2639604730654881 1104 60-61 0.23473237393174884 1104 62-63 0.1807841312907783 1104 64-65 0.3975798160467079 1104 66-67 0.20127644891476137 1104 68-69 0.12336623433105842 1104 70-71 -0.022656256576745193 1104 72-73 6.223847683486383E-4 1104 74-75 -0.01552700475076918 1104 76-77 -0.09378054254312218 1104 78-79 0.08854660449980756 1104 80-81 0.06662711429822821 1104 82-83 0.11279809363571758 1104 84-85 0.07240108380953814 1104 86-87 0.06362931340560252 1104 88-89 0.1951062490826203 1104 90-91 0.0649043680474044 1104 92-93 0.009944148747408121 1104 94-95 0.18850397929822904 1104 96-97 -0.024794755506757582 1104 98-99 -0.04888562620322645 1104 100-101 0.22422439030097152 1105 1 0.16444152491419572 1105 2 0.27223303209362015 1105 3 0.2651205302591997 1105 4 0.24903051097633977 1105 5 0.28215778776842626 1105 6 0.16662920249691382 1105 7 0.07486890173399274 1105 8 0.22169031998649302 1105 9 0.14965461487730636 1105 10-11 0.16139478488340586 1105 12-13 0.2681327472968178 1105 14-15 0.18041459212493294 1105 16-17 0.2924126185208067 1105 18-19 0.33549021762395626 1105 20-21 0.31654448638122545 1105 22-23 0.1420252836323641 1105 24-25 0.02431280580749018 1105 26-27 -0.08627171324317828 1105 28-29 0.05807480929741615 1105 30-31 -0.20482184446080964 1105 32-33 0.06465812795158143 1105 34-35 -0.2458311078427684 1105 36-37 -0.09432986192769022 1105 38-39 0.07175653614513777 1105 40-41 -0.04130739001831074 1105 42-43 -0.07670563405790887 1105 44-45 -0.09411277272435825 1105 46-47 -0.1484657684808397 1105 48-49 0.005274743467033716 1105 50-51 -0.10634596597358126 1105 52-53 -0.0787167858040263 1105 54-55 -0.2011859299290535 1105 56-57 -0.039951856617904014 1105 58-59 0.10001799245690535 1105 60-61 -0.001268141815501167 1105 62-63 -0.007551158715259021 1105 64-65 -0.0049597867826634 1105 66-67 -0.15711270048682024 1105 68-69 -0.0407771162617081 1105 70-71 -0.3044711892694707 1105 72-73 -0.27013293613727996 1105 74-75 -0.2215273169135834 1105 76-77 -0.07927243648920168 1105 78-79 -0.34143414546003825 1105 80-81 -0.2799914611988008 1105 82-83 -0.1650885641184736 1105 84-85 -0.163935572474319 1105 86-87 -0.18051096080001727 1105 88-89 -0.46245318060949003 1105 90-91 -0.3713852376095268 1105 92-93 -0.32083985494425704 1105 94-95 -0.3244023695957452 1105 96-97 -0.5727036039652837 1105 98-99 -0.9670507874760368 1105 100-101 -0.9528006528476176 1106 1 -0.009354182238759279 1106 2 0.1678751926784834 1106 3 0.11659251628115541 1106 4 0.17905358597773358 1106 5 0.0319879157477132 1106 6 0.12459209821002304 1106 7 0.17801805060002351 1106 8 0.20260355394079 1106 9 0.24638040866869204 1106 10-11 0.16673435892962374 1106 12-13 0.20907986203471296 1106 14-15 0.30759342560305214 1106 16-17 0.17277615203622076 1106 18-19 0.07333346627667936 1106 20-21 0.1881505567375541 1106 22-23 0.01823970228078764 1106 24-25 0.21390525585231046 1106 26-27 0.2114100941655508 1106 28-29 0.24455733255842205 1106 30-31 0.2667606236626341 1106 32-33 0.1911466330728686 1106 34-35 0.18217345886900915 1106 36-37 0.1797906099790012 1106 38-39 0.15585390348605443 1106 40-41 0.23695677661837777 1106 42-43 0.043922224756386186 1106 44-45 0.060114268930384185 1106 46-47 0.043019294853458234 1106 48-49 -0.15955581073211533 1106 50-51 -0.025622880279733806 1106 52-53 0.0017695384512350643 1106 54-55 -0.00958827556723918 1106 56-57 0.1306876062169522 1106 58-59 -0.06072651756024783 1106 60-61 0.029070089514959818 1106 62-63 0.257567697497862 1106 64-65 0.3820224731089681 1106 66-67 0.16843353845548137 1106 68-69 0.16644728578631174 1106 70-71 0.09007314720475534 1106 72-73 0.013126625846659579 1106 74-75 0.12952855040934708 1106 76-77 0.24501411334230738 1106 78-79 0.38455156804433344 1106 80-81 0.1312922292640728 1106 82-83 0.1647629881841226 1106 84-85 0.14099147987286997 1106 86-87 0.3747507850063947 1106 88-89 0.3598769461379483 1106 90-91 0.41280708067668215 1106 92-93 0.29791633201893575 1106 94-95 0.5483303501452355 1106 96-97 0.3729730730374179 1106 98-99 0.19188709233390355 1106 100-101 0.19915682962686176 1107 1 0.2537891356291446 1107 2 0.36224954050950586 1107 3 0.34691222716034886 1107 4 0.3700643782416577 1107 5 0.4660840264540198 1107 6 0.3415111397319208 1107 7 0.20301103192304026 1107 8 0.385375311974677 1107 9 0.4307917512834436 1107 10-11 0.2979476461100816 1107 12-13 0.4151192780591515 1107 14-15 0.4974679765564787 1107 16-17 0.57118411594913 1107 18-19 0.4484034501945757 1107 20-21 0.561039747109497 1107 22-23 0.4927517622340858 1107 24-25 0.4869561327406515 1107 26-27 0.3463158275185805 1107 28-29 0.42919235324121274 1107 30-31 0.3679247773995158 1107 32-33 0.2792812012606021 1107 34-35 0.33127314788347206 1107 36-37 0.42985024844816166 1107 38-39 0.2375526559196004 1107 40-41 0.39083430489729665 1107 42-43 0.40671993701930376 1107 44-45 0.4501141553332957 1107 46-47 0.4383779884653549 1107 48-49 0.3419574647099779 1107 50-51 0.14668766818321188 1107 52-53 0.32066955083872273 1107 54-55 0.29053278646811975 1107 56-57 0.2935777107749544 1107 58-59 0.32493909914479957 1107 60-61 0.4680925271028258 1107 62-63 0.3329353057761679 1107 64-65 0.33402797553534214 1107 66-67 0.36077476894991634 1107 68-69 0.19871507847055625 1107 70-71 0.30030077953318113 1107 72-73 0.3103214810888133 1107 74-75 0.3239450914430435 1107 76-77 0.32149037506782463 1107 78-79 0.11440390806312095 1107 80-81 0.18997080306891334 1107 82-83 0.047654942751783835 1107 84-85 0.04351760855456277 1107 86-87 0.06619135290526756 1107 88-89 0.06891667752952912 1107 90-91 0.24541528595635143 1107 92-93 0.1100976178189228 1107 94-95 0.058351776719057113 1107 96-97 -0.153882359526758 1107 98-99 -0.35146480059922425 1107 100-101 -0.38416798392516327 1108 1 -0.043385045919642096 1108 2 0.15827997016920037 1108 3 -0.11226166083608646 1108 4 0.08611223539653423 1108 5 0.12172217503211868 1108 6 0.031036600176904017 1108 7 0.003798053892474229 1108 8 0.11745600058119265 1108 9 0.049745138535264743 1108 10-11 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-1.2856496792475127 2316 66-67 -1.0472923057563008 2316 68-69 -0.6763371327992473 2316 70-71 -1.1033417199126632 2316 72-73 -1.7076701078075622 2316 74-75 -1.779173108840137 2316 76-77 -1.7486615917702935 2316 78-79 -2.0155682258208962 2316 80-81 -2.0293493270332803 2316 82-83 -1.60297702094989 2316 84-85 -1.5867108694699574 2316 86-87 -1.7184301851294173 2316 88-89 -1.6406107294288574 2316 90-91 -1.7481960902950213 2316 92-93 -2.023826362572258 2316 94-95 -1.6416437749561545 2316 96-97 -1.789230557804423 2316 98-99 -1.5317305239194496 2316 100-101 -1.64465660601509 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2220.0 3 793.0 4 841.0 5 905.0 6 986.0 7 1183.0 8 1402.0 9 1828.0 10 2238.0 11 2918.0 12 3870.0 13 4916.0 14 5656.0 15 6820.0 16 8854.0 17 11821.0 18 10618.0 19 6427.0 20 6347.0 21 6595.0 22 7065.0 23 7853.0 24 8900.0 25 10388.0 26 11967.0 27 14452.0 28 17250.0 29 20730.0 30 25900.0 31 32174.0 32 41469.0 33 54404.0 34 75119.0 35 108062.0 36 165343.0 37 246072.0 38 222942.0 39 38308.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2521725745301 19.107613129477745 14.439715030501374 22.200499265490784 2 15.26162632649152 20.62999094133953 40.692456385853774 23.41592634631518 3 19.183371322222452 25.612596976223834 29.94617427490015 25.257857426653562 4 12.617742620904732 17.047123817554567 37.82420001940767 32.51093354213304 5 13.565934613109013 37.7931610470405 33.85818139518925 14.782722944661236 6 30.76384344924417 36.59634143688385 18.092750984424917 14.547064129447065 7 27.7859057848267 33.428276263012826 21.06001480607802 17.725803146082452 8 26.16376273381945 34.62134511976945 20.11939289366267 19.09549925274843 9 26.6390418488382 17.044479135816722 20.691925555215334 35.624553460129746 10-11 24.520594414822977 27.170338989785925 27.473762099383407 20.83530449600769 12-13 25.824083881774357 24.718960580853842 28.03014710434854 21.42680843302326 14-15 22.82567415185189 25.64286598607361 26.78722599771797 24.74423386435653 16-17 21.771929215517577 29.180760252678635 27.06687919801344 21.98043133379035 18-19 21.602467715794425 27.52046844774403 29.858440947351912 21.018622889109636 20-21 22.637247369607522 26.493678567858623 29.906779317212557 20.962294745321294 22-23 22.157960771193174 26.671364643887753 29.746267408305798 21.424407176613276 24-25 21.949603869098024 26.776441659296058 29.85603793823745 21.417916533368462 26-27 21.8266580130607 26.952079541804945 29.71865465421261 21.502607790921743 28-29 21.546127947254387 27.013935383613013 29.927849481296608 21.51208718783599 30-31 21.796005178781716 26.83109911947329 29.86673335296007 21.506162348784926 32-33 21.607736754308455 27.022854220342435 29.679122581271088 21.690286444078023 34-35 21.97268761374585 26.99331241301788 29.545024957178036 21.488975016058237 36-37 22.021447783963175 26.804753572957928 29.52093760036399 21.652861042714914 38-39 21.847065357028157 26.989966946793704 29.601730609677762 21.561237086500377 40-41 21.99610587731506 26.85372283481426 29.60796154052029 21.54220974735039 42-43 21.85995454345867 27.010511041088613 29.310060030234663 21.81947438521805 44-45 21.987124131485242 27.20015723697829 29.0363589603123 21.776359671224167 46-47 22.033491462370197 27.32982449684188 28.697367452628196 21.939316588159727 48-49 22.18958975510595 27.49591327486253 28.36653523072799 21.94796173930353 50-51 22.11104113505073 27.70265805877487 28.272391433257475 21.913909372916923 52-53 22.32779057654679 27.798647507683093 28.045167271709943 21.82839464406017 54-55 22.05530889350849 27.726082598260916 28.07092157652385 22.147686931706744 56-57 22.07526741875818 28.044939816927034 27.886322302592703 21.993470461722076 58-59 22.2367658411374 27.72622459826854 27.973164095791613 22.063845464802444 60-61 22.190100011918627 27.604167755722507 28.402464855732333 21.803267376626525 62-63 22.13710131024126 27.66795790801338 28.3669679191415 21.827972862603865 64-65 22.0539288684924 27.62011340460117 28.26797242124553 22.057985305660903 66-67 21.982741134160015 27.920583955770862 27.985243013078616 22.11143189699051 68-69 21.87871715726869 27.834700996260885 28.19196633959865 22.094615506871772 70-71 21.944130956195394 27.80961926027184 28.10727739847791 22.13897238505485 72-73 22.043187048235353 27.75404428956101 27.973835815938923 22.228932846264716 74-75 21.87234142224133 28.095243273719845 27.925136123363863 22.107279180674958 76-77 22.02897216981724 27.975210932957413 27.782244401962714 22.21357249526264 78-79 21.959069733202053 28.056155308813207 27.729026040677628 22.25574891730711 80-81 21.921444114249567 28.223336321961902 27.66873165141741 22.18648791237112 82-83 22.057531601299864 28.137246863296976 27.689582698853084 22.115638836550076 84-85 22.01908115975975 27.912156396914895 27.679183885161702 22.38957855816366 86-87 22.053640327488317 27.840396499262837 27.75444650083127 22.351516672417578 88-89 22.12396894001052 27.699953073752837 27.744118953435947 22.431959032800695 90-91 22.015949403622812 27.784086987620704 27.89066731919931 22.309296289557174 92-93 22.007854450046267 27.82284985684955 27.8074973687425 22.36179832436168 94-95 21.826744319785863 27.78636330367319 27.849642928094188 22.537249448446765 96-97 21.88663966595296 27.794115863260892 27.73518163996083 22.584062830825317 98-99 21.71167666424048 27.80467501610488 27.646387069246792 22.83726125040785 100-101 21.8239178154286 27.881875576019517 27.63011085930921 22.664095749242676 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 181.0 2 162.0 3 215.0 4 194.0 5 210.0 6 352.5 7 751.0 8 1277.5 9 1501.5 10 1433.0 11 1356.5 12 1385.0 13 1508.5 14 1676.0 15 1743.5 16 1819.0 17 1958.0 18 2047.5 19 2177.5 20 2378.0 21 2751.5 22 3298.0 23 3987.5 24 4939.0 25 6075.5 26 7731.0 27 9486.5 28 10999.5 29 12828.0 30 15027.0 31 17409.0 32 19706.5 33 22055.5 34 25084.5 35 27385.5 36 30004.5 37 33149.0 38 34695.0 39 35548.0 40 36676.0 41 38625.0 42 40289.5 43 41762.5 44 43442.5 45 45104.0 46 45555.0 47 45563.0 48 47786.0 49 47485.0 50 44259.5 51 42046.0 52 40067.5 53 38691.0 54 37077.5 55 33826.0 56 30166.5 57 26897.0 58 24212.0 59 21497.0 60 18277.5 61 14878.5 62 11652.0 63 8830.5 64 6543.0 65 5036.0 66 3686.0 67 2768.5 68 2046.0 69 1278.0 70 843.5 71 618.0 72 463.0 73 332.5 74 239.5 75 178.5 76 124.0 77 65.0 78 40.5 79 26.0 80 17.5 81 17.5 82 12.0 83 5.5 84 5.5 85 6.0 86 4.0 87 3.0 88 3.5 89 1.5 90 0.5 91 1.5 92 1.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024421903436462902 2 0.008949122149662777 3 0.010287308639331978 4 0.020407343967455305 5 0.05846202226742319 6 0.04466197409270956 7 0.016141874531634726 8 0.05034926667380367 9 0.028938282839096458 10-11 0.024045538486243443 12-13 0.03696740177711166 14-15 0.018400064232951506 16-17 0.018107335938336366 18-19 0.017605516004710418 20-21 0.01267095332405524 22-23 0.008196392249223852 24-25 0.0017145514398886627 26-27 0.0 28-29 0.0018818247510973129 30-31 0.0 32-33 3.763649502194626E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 4.181832780216251E-5 44-45 4.181832780216251E-5 46-47 0.0 48-49 7.109115726367627E-4 50-51 8.363665560432502E-5 52-53 2.9272829461513754E-4 54-55 0.001463641473075688 56-57 3.763649502194626E-4 58-59 9.200032116475752E-4 60-61 0.0033872845519751634 62-63 0.002383644684723263 64-65 0.001463641473075688 66-67 0.012252770046033616 68-69 0.014887324697569855 70-71 0.026512819826571034 72-73 0.01647642115405203 74-75 0.018818247510973128 76-77 0.030360105984369983 78-79 0.02082552724547693 80-81 0.047045618777432824 82-83 0.03713467508832031 84-85 0.019069157477786106 86-87 0.015514599614602291 88-89 0.012963681618670378 90-91 0.02555099828712129 92-93 0.03362193555293866 94-95 0.013423683224494165 96-97 0.02070007226207044 98-99 0.029941922706348358 100-101 0.04052195964029547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1195648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8699170958958 #Duplication Level Percentage of deduplicated Percentage of total 1 83.4181415184694 55.781642076337526 2 10.315190794538944 13.795519065183335 3 2.7516498862487255 5.520077993111501 4 1.140986124736027 3.0519059027466215 5 0.6039897056624206 2.0194370772210286 6 0.3750819357923666 1.50490187703622 7 0.2463014221030146 1.1529108973640878 8 0.17805384340828037 0.9525156597853857 9 0.14829039233550895 0.892454961743401 >10 0.7453314967703362 9.15047340935187 >50 0.05591116140931888 2.5889974509086375 >100 0.020319159998644615 2.3882765462522526 >500 2.5085284235563075E-4 0.09586631762964168 >1k 3.762792635334461E-4 0.6502638325307192 >5k 1.2542642117781538E-4 0.45475693279782786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5422 0.4534779466866502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.33747390536345145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2645 0.22121895407343967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.363665560432502E-5 0.0 0.0 0.0 0.0 7 3.3454662241730006E-4 0.0 0.0 0.0 0.0 8 3.3454662241730006E-4 0.0 0.0 0.0 0.0 9 3.3454662241730006E-4 0.0 0.0 0.0 0.0 10-11 3.763649502194626E-4 0.0 0.0 0.0 0.0 12-13 4.1818327802162507E-4 0.0 0.0 0.0 0.0 14-15 4.1818327802162507E-4 0.0 0.0 0.0 0.0 16-17 5.018199336259501E-4 0.0 0.0 1.2545498340648753E-4 0.0 18-19 5.436382614281126E-4 0.0 0.0 2.5090996681297506E-4 0.0 20-21 5.854565892302752E-4 0.0 0.0 2.5090996681297506E-4 0.0 22-23 5.854565892302752E-4 0.0 0.0 4.6000160582378765E-4 0.0 24-25 5.854565892302752E-4 0.0 0.0 7.945482282410877E-4 0.0 26-27 5.854565892302752E-4 0.0 0.0 0.001296368161867038 0.0 28-29 6.690932448346001E-4 0.0 0.0 0.0023000080291189383 0.0 30-31 6.690932448346001E-4 0.0 0.0 0.0046418343860400385 0.0 32-33 6.690932448346001E-4 0.0 0.0 0.006816387431752489 0.0 34-35 6.690932448346001E-4 0.0 0.0 0.008781848838454127 8.363665560432502E-5 36-37 6.690932448346001E-4 0.0 0.0 0.01200186007922064 8.363665560432502E-5 38-39 6.690932448346001E-4 0.0 0.0 0.01748006102130393 8.363665560432502E-5 40-41 6.690932448346001E-4 0.0 0.0 0.02366917353602398 8.363665560432502E-5 42-43 6.690932448346001E-4 0.0 0.0 0.032200112407665134 8.363665560432502E-5 44-45 6.690932448346001E-4 0.0 0.0 0.039601956428647896 8.363665560432502E-5 46-47 7.945482282410877E-4 0.0 0.0 0.05277472968632908 8.363665560432502E-5 48-49 9.200032116475752E-4 0.0 0.0 0.07025479070763302 8.363665560432502E-5 50-51 9.200032116475752E-4 0.0 0.0 0.0785348196124612 8.363665560432502E-5 52-53 9.200032116475752E-4 0.0 0.0 0.09229304945937267 8.363665560432502E-5 54-55 9.200032116475752E-4 0.0 0.0 0.11106947864254363 8.363665560432502E-5 56-57 0.0010036398672519002 0.0 0.0 0.14444050422866933 8.363665560432502E-5 58-59 0.0010036398672519002 0.0 0.0 0.19090066641687187 8.363665560432502E-5 60-61 0.0010036398672519002 0.0 0.0 0.24421903436462905 8.363665560432502E-5 62-63 0.0010036398672519002 0.0 0.0 0.30765743764050957 8.363665560432502E-5 64-65 0.0010036398672519002 0.0 0.0 0.3782467749705599 8.363665560432502E-5 66-67 0.0010872765228562252 0.0 0.0 0.47543256878278556 8.363665560432502E-5 68-69 0.0010872765228562252 0.0 0.0 0.6013057354672947 8.363665560432502E-5 70-71 0.0010872765228562252 0.0 0.0 0.6636986805481212 8.363665560432502E-5 72-73 0.0010872765228562252 0.0 0.0 0.742902593405417 8.363665560432502E-5 74-75 0.0010872765228562252 0.0 0.0 0.834024729686329 8.363665560432502E-5 76-77 0.0010872765228562252 0.0 0.0 0.9357269029011883 8.363665560432502E-5 78-79 0.0010872765228562252 0.0 0.0 1.0339999732362704 8.363665560432502E-5 80-81 0.0010872765228562252 0.0 0.0 1.1915296140670164 8.363665560432502E-5 82-83 0.0011709131784605504 0.0 0.0 1.3670829541804945 8.363665560432502E-5 84-85 0.0012127315062627128 0.0 0.0 1.6295347794668664 8.363665560432502E-5 86-87 0.0012545498340648753 0.0 0.0 2.0043942698854513 8.363665560432502E-5 88-89 0.0012545498340648753 0.0 0.0 2.397653824536987 8.363665560432502E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3660 0.0 40.771748 1 GTATCAA 6165 0.0 39.15988 1 TATCAAC 7560 0.0 31.87776 2 ATCAACG 7565 0.0 31.856693 3 TCAACGC 7695 0.0 31.318504 4 CAACGCA 7745 0.0 31.054945 5 AACGCAG 8035 0.0 30.046133 6 ACGCAGA 9170 0.0 26.274302 7 CGCAGAG 9160 0.0 26.199228 8 GTACATG 5950 0.0 26.038225 1 GCAGAGT 9650 0.0 24.42672 9 TACATGG 6180 0.0 24.382174 2 ACATGGG 6175 0.0 23.70912 3 GAGTACT 4965 0.0 23.114332 12-13 CATGGGG 2635 0.0 21.64723 4 GTACTTT 5265 0.0 21.38843 14-15 AGTACTT 5115 0.0 20.764208 12-13 AGAGTAC 8580 0.0 20.216137 10-11 CATGGGA 3815 0.0 19.686306 4 CAGAGTA 9490 0.0 19.604609 10-11 >>END_MODULE