FastQCFastQC Report
Thu 26 May 2016
SRR936861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195648
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68810.5755038272133605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.4527252167862113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.2971610373621668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.11817859436891126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.043.0975151
GTATCAA66400.037.1387561
ATCAACG85150.028.8347363
TCAACGC85650.028.7773024
TATCAAC85850.028.5443042
CAACGCA87150.028.2345855
AACGCAG90650.027.1968486
ACGCAGA102050.023.9724967
CGCAGAG102650.023.8323758
GTACATG56900.022.9640521
GTATAGG3750.022.8070581
TACATGG56650.022.6346572
AGTACTT62250.022.46756612-13
GAGTACT60000.022.36028112-13
GCAGAGT108700.022.112619
ACATGGG56850.021.5525823
TATAGGG2457.536073E-921.322432
GTACTTT64550.020.71057914-15
AGAGTAC97600.020.63645410-11
TACTTTT71850.019.102114-15
Produced by FastQC (version 0.11.5)