FastQCFastQC Report
Thu 26 May 2016
SRR936860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213895
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.4616544264536883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.34516988701658713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.21929409051029947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66800.037.578061
GGTATCA40900.036.731671
TCAACGC81600.030.3596084
TATCAAC82300.030.2747142
ATCAACG82250.030.2353063
CAACGCA83250.029.6995415
AACGCAG86150.028.8077966
GTACATG63450.026.0000821
ACGCAGA95500.025.881377
CGCAGAG96100.025.6219168
TACATGG63400.024.9748882
ACATGGG63650.023.9056283
GCAGAGT101100.023.648539
GAGTACT49600.023.52940412-13
CATGGGG24150.022.6427564
GTACTTT54750.021.0968914-15
AGTACTT52500.020.96200412-13
AGAGTAC88750.019.7354710-11
CAGAGTA99150.019.2713310-11
TACCTGG8150.018.6698552
Produced by FastQC (version 0.11.5)