FastQCFastQC Report
Thu 26 May 2016
SRR936860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213895
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69480.5723724045325171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.4528398255203292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36230.29846074001458117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.11854402563648422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.037.5861051
GTATCAA68350.036.154271
CGTAATC650.00584156129.2334692
GTACCCG1004.937718E-528.5132051
ATCAACG87000.028.1192553
TATCAAC87200.028.001442
TCAACGC87800.027.8089394
CAACGCA89950.027.1442475
AACGCAG92850.026.2953626
GTACATG58050.024.9684961
TACATGG59450.023.8920292
ACGCAGA102500.023.6807257
ACATGGG58950.023.2878763
CGCAGAG104150.023.2628298
GCAGAGT109000.021.8790689
GAGTACT62800.020.87858412-13
AGTACTT65250.020.786312-13
AGAGTAC96850.020.69971810-11
CTCGCGC958.2611605E-619.99896260-61
GTACTTT66500.019.67917314-15
Produced by FastQC (version 0.11.5)