FastQCFastQC Report
Thu 26 May 2016
SRR936859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276068
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76780.3373361428568918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55460.243665830722105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.15724486263152065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103900.032.4365921
GGTATCA67750.030.940961
TCAACGC124100.026.8875164
TATCAAC125600.026.8324972
ATCAACG125750.026.6103153
CAACGCA126050.026.4715675
AACGCAG130450.025.687446
ACGCAGA146100.022.7081137
CGCAGAG146400.022.4338198
GTACATG89400.022.2782461
TACATGG87800.022.1969782
GAGTACT67900.021.45209112-13
GCAGAGT150400.021.1419249
ACATGGG87900.020.7647973
GTACTTT71750.020.16720214-15
AGTACTT73000.019.95338212-13
GTATAGG9150.019.7409021
AGAGTAC121550.019.43210810-11
CATGGGG42350.017.5088084
GTCCTAC6450.016.9501131
Produced by FastQC (version 0.11.5)