##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936859_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276068 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57848095926835 33.0 31.0 34.0 30.0 34.0 2 31.675625684294143 33.0 31.0 34.0 30.0 34.0 3 31.687107327197605 33.0 31.0 34.0 30.0 34.0 4 35.25947774846797 37.0 35.0 37.0 33.0 37.0 5 35.28335445162447 37.0 35.0 37.0 33.0 37.0 6 35.32990314876357 37.0 35.0 37.0 33.0 37.0 7 35.258800264315475 37.0 35.0 37.0 32.0 37.0 8 35.23173077430024 37.0 35.0 37.0 32.0 37.0 9 36.82072547920361 39.0 37.0 39.0 33.0 39.0 10-11 36.68354680088644 39.0 37.0 39.0 32.0 39.0 12-13 36.5557450392519 39.0 37.0 39.0 32.0 39.0 14-15 37.68889330195759 40.0 37.0 41.0 32.0 41.0 16-17 37.61033589506113 40.0 37.0 41.0 32.0 41.0 18-19 37.556548618055345 40.0 37.0 41.0 32.0 41.0 20-21 37.48582863077904 40.0 37.0 41.0 32.0 41.0 22-23 37.34062646634459 40.0 37.0 41.0 31.5 41.0 24-25 37.15555049321901 39.5 36.5 41.0 31.0 41.0 26-27 37.11955266714351 39.5 36.5 41.0 31.0 41.0 28-29 37.19910740803877 40.0 37.0 41.0 31.0 41.0 30-31 37.08442849686389 39.5 36.5 41.0 31.0 41.0 32-33 36.90743488331631 39.0 36.0 41.0 30.0 41.0 34-35 36.717511295796086 39.0 36.0 41.0 30.0 41.0 36-37 36.56982568183376 39.0 36.0 41.0 30.0 41.0 38-39 36.40993261185518 39.0 35.0 41.0 30.0 41.0 40-41 36.212273315208506 39.0 35.0 41.0 30.0 41.0 42-43 36.01067784442293 39.0 35.0 40.0 29.0 41.0 44-45 35.77960763913907 39.0 35.0 40.0 28.0 41.0 46-47 35.46230341097015 38.5 35.0 40.0 26.5 41.0 48-49 35.14492471226694 38.0 34.5 40.0 26.0 41.0 50-51 34.5044097979498 37.5 33.5 39.5 24.5 40.5 52-53 34.814191403771765 38.0 34.0 40.0 26.0 40.5 54-55 35.11942108056526 39.0 35.0 40.0 26.0 41.0 56-57 35.004491957182296 38.5 34.5 40.0 26.0 41.0 58-59 34.74956240323224 38.0 34.0 40.0 25.5 41.0 60-61 34.459134129560276 38.0 34.0 40.0 24.5 41.0 62-63 34.14199443953344 37.5 34.0 40.0 23.5 41.0 64-65 33.82686545393196 37.0 33.5 40.0 23.0 41.0 66-67 33.45186237845266 36.5 33.0 39.5 22.0 41.0 68-69 33.010008488322846 36.0 32.5 39.0 21.5 41.0 70-71 32.65179994622305 35.5 32.0 39.0 20.5 40.0 72-73 32.268242425094506 35.0 32.0 38.0 20.0 40.0 74-75 31.83262494793653 35.0 31.5 37.0 18.5 39.5 76-77 31.375908145099356 35.0 31.0 37.0 17.5 39.0 78-79 30.94536125458466 35.0 31.0 36.0 13.0 39.0 80-81 30.520127034868906 34.5 30.5 36.0 9.0 37.5 82-83 30.11104303562108 34.0 30.0 35.0 7.0 37.0 84-85 29.75735259227756 34.0 30.0 35.0 4.5 37.0 86-87 29.414203573882677 34.0 29.5 35.0 2.0 36.0 88-89 29.04100624410167 34.0 29.0 35.0 2.0 36.0 90-91 28.69879480753651 34.0 29.0 35.0 2.0 35.5 92-93 28.33274577033727 34.0 29.0 35.0 2.0 35.0 94-95 27.930752068918856 34.0 28.5 35.0 2.0 35.0 96-97 27.469494320907813 34.0 27.0 35.0 2.0 35.0 98-99 26.844001145835712 34.0 25.5 35.0 2.0 35.0 100-101 24.61924951275621 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5301594857943925 1101 2 0.38888080800844094 1101 3 0.4206480651062101 1101 4 0.38909157802011407 1101 5 0.4272492277078541 1101 6 0.322261174966755 1101 7 0.2977061967264376 1101 8 0.22449460414837574 1101 9 0.3095978928847032 1101 10-11 0.3619012200601617 1101 12-13 0.4304676423056364 1101 14-15 0.7252088347220749 1101 16-17 0.698990685300096 1101 18-19 0.8004356127573047 1101 20-21 0.6442468067274021 1101 22-23 0.6464761117176536 1101 24-25 0.7709728453968552 1101 26-27 0.739262168773891 1101 28-29 0.7996405196203042 1101 30-31 0.6790059389802607 1101 32-33 0.675525688601752 1101 34-35 0.6934107685196054 1101 36-37 0.6324775729123147 1101 38-39 0.7702604057284432 1101 40-41 0.7940202635605402 1101 42-43 0.7217240138200793 1101 44-45 0.776954058170098 1101 46-47 0.958156062950728 1101 48-49 0.8342135503214223 1101 50-51 0.8220667529902528 1101 52-53 0.8473708424148398 1101 54-55 0.8726666363549995 1101 56-57 0.7498252244239083 1101 58-59 0.8274147787196071 1101 60-61 0.9945193857654075 1101 62-63 0.9606899932499502 1101 64-65 0.8558435463231575 1101 66-67 0.8251587881603655 1101 68-69 0.8542091675571797 1101 70-71 1.0258546024634185 1101 72-73 0.9991795823479848 1101 74-75 0.9044598141648841 1101 76-77 0.8804598591922215 1101 78-79 0.7416486904375077 1101 80-81 0.8120433161804428 1101 82-83 0.755152918138883 1101 84-85 0.8851274821950561 1101 86-87 0.9315404619408305 1101 88-89 1.0331583574311196 1101 90-91 1.1707746591176296 1101 92-93 0.9938627924471461 1101 94-95 1.03263265760787 1101 96-97 1.2111117028512695 1101 98-99 1.230054077537627 1101 100-101 1.4575237123124225 1102 1 0.32576437957697024 1102 2 0.37185057383549847 1102 3 0.3207469271855352 1102 4 0.1663008285123837 1102 5 0.18194168769502994 1102 6 0.19536305394434095 1102 7 0.2638542577916638 1102 8 0.2797830077002317 1102 9 0.289578670699413 1102 10-11 0.45797406899255577 1102 12-13 0.35910223916904016 1102 14-15 0.5193670806348578 1102 16-17 0.4544444969326449 1102 18-19 0.4658440921692062 1102 20-21 0.3652194030521301 1102 22-23 0.4250932347733354 1102 24-25 0.5777971597667033 1102 26-27 0.5210680374613688 1102 28-29 0.6134980166812554 1102 30-31 0.5435214300640325 1102 32-33 0.6123360937010389 1102 34-35 0.3780428287403552 1102 36-37 0.4981897158031998 1102 38-39 0.4097061760203431 1102 40-41 0.445417001929286 1102 42-43 0.5429680572252948 1102 44-45 0.6756141470562582 1102 46-47 0.5241474478551567 1102 48-49 0.6178767462160693 1102 50-51 0.5211334941639905 1102 52-53 0.573514609289326 1102 54-55 0.5867385239787737 1102 56-57 0.6387851072327706 1102 58-59 0.5534743164581499 1102 60-61 0.5257288224588876 1102 62-63 0.5709376588070469 1102 64-65 0.6841213704961788 1102 66-67 0.37960938592807025 1102 68-69 0.32392724815360197 1102 70-71 0.545209461108989 1102 72-73 0.5806839805043893 1102 74-75 0.6091716019975699 1102 76-77 0.6078926807247385 1102 78-79 0.5678197894174097 1102 80-81 0.5572751994900926 1102 82-83 0.61868915829778 1102 84-85 0.6125815354505946 1102 86-87 0.6390760689358572 1102 88-89 0.5282049864814198 1102 90-91 0.2922721474945078 1102 92-93 0.40008187312749754 1102 94-95 0.5761522074395202 1102 96-97 0.36445438345896974 1102 98-99 0.43032175673486606 1102 100-101 0.5890404806886771 1103 1 0.47873018369242715 1103 2 0.5667216603467331 1103 3 0.42578376631364634 1103 4 0.4186566446804605 1103 5 0.44383881739976516 1103 6 0.31213027171840935 1103 7 0.4062179976847844 1103 8 0.47001077632145893 1103 9 0.6768279294688995 1103 10-11 0.6288516769059243 1103 12-13 0.5037144439577261 1103 14-15 0.553678105047517 1103 16-17 0.7056315542728626 1103 18-19 0.7981891807171095 1103 20-21 0.6937397271628996 1103 22-23 0.6629715494784634 1103 24-25 0.9183078890759688 1103 26-27 0.8529675806929617 1103 28-29 0.8693501624492725 1103 30-31 1.0030042071288463 1103 32-33 0.8435893147084101 1103 34-35 0.7174252436259962 1103 36-37 0.776698704220145 1103 38-39 0.8167946818895686 1103 40-41 0.833389568048851 1103 42-43 0.9240132757583268 1103 44-45 0.8520365795685336 1103 46-47 0.9114145359443739 1103 48-49 0.8969023157126941 1103 50-51 0.9022125884045238 1103 52-53 0.967697690503563 1103 54-55 0.8688756851231716 1103 56-57 1.0221745997953278 1103 58-59 1.1331905187304656 1103 60-61 1.1926522626092435 1103 62-63 1.1413498651722236 1103 64-65 1.1525818669169894 1103 66-67 1.112321983674967 1103 68-69 1.2536563626069466 1103 70-71 1.0618734002025363 1103 72-73 1.1369208454568636 1103 74-75 1.239653320188463 1103 76-77 1.146783025153411 1103 78-79 1.148297585157966 1103 80-81 1.024933658038428 1103 82-83 1.085047390244842 1103 84-85 1.2105858340057445 1103 86-87 1.1770515815851006 1103 88-89 1.1912265269271352 1103 90-91 1.2063206567569331 1103 92-93 1.1413915811733233 1103 94-95 1.0708874908374924 1103 96-97 1.0243978326103154 1103 98-99 1.340205618422889 1103 100-101 1.749045394073839 1104 1 0.3999729665643592 1104 2 0.4288035526646432 1104 3 0.48343624882771863 1104 4 0.535466309764189 1104 5 0.40794386014726314 1104 6 0.3760368260628155 1104 7 0.3747084735415456 1104 8 0.3752189370017689 1104 9 0.5394217090284883 1104 10-11 0.5916962830679253 1104 12-13 0.5697426142565547 1104 14-15 0.5905405681206233 1104 16-17 0.5642224720101225 1104 18-19 0.6147765150582742 1104 20-21 0.6549290891040656 1104 22-23 0.7211521416658186 1104 24-25 0.6421385113165243 1104 26-27 0.7212177050031343 1104 28-29 0.7297073785391035 1104 30-31 0.6339505626066284 1104 32-33 0.6382980580984565 1104 34-35 0.6434974907349726 1104 36-37 0.667657822580118 1104 38-39 0.6778630893807289 1104 40-41 0.6214269059682209 1104 42-43 0.7055369829417728 1104 44-45 0.6785184847902244 1104 46-47 0.6659895397543352 1104 48-49 0.5337270315049523 1104 50-51 0.6179241153784361 1104 52-53 0.7904285060413514 1104 54-55 0.7659412975357256 1104 56-57 0.7981652527083369 1104 58-59 0.8225194588439209 1104 60-61 0.788576427654597 1104 62-63 0.6998560183868889 1104 64-65 0.7215887537563574 1104 66-67 0.6360345436933201 1104 68-69 0.782702580036478 1104 70-71 0.7927492854374449 1104 72-73 0.810008072451879 1104 74-75 0.7085401795739124 1104 76-77 0.8289547211446688 1104 78-79 0.8113777039469525 1104 80-81 0.9408299909531266 1104 82-83 0.8754440259164689 1104 84-85 1.1011811063998387 1104 86-87 1.0385348198271807 1104 88-89 1.071110822982991 1104 90-91 1.196291393344321 1104 92-93 0.8751547811341673 1104 94-95 0.9776116609257031 1104 96-97 0.766176026176737 1104 98-99 1.0432950739165072 1104 100-101 1.1314983035993933 1105 1 0.39246029883980427 1105 2 0.34947233776704323 1105 3 0.3435639521965079 1105 4 0.4466869627817971 1105 5 0.349997852679671 1105 6 0.42528845982410957 1105 7 0.41523644296059814 1105 8 0.4443288611447258 1105 9 0.39225528324877246 1105 10-11 0.4160697249820231 1105 12-13 0.42667573631691624 1105 14-15 0.539990160933236 1105 16-17 0.6657213727553781 1105 18-19 0.5952868302122098 1105 20-21 0.6730298462289639 1105 22-23 0.5718681360458149 1105 24-25 0.7976304199685487 1105 26-27 0.5809627281151606 1105 28-29 0.5204747608673088 1105 30-31 0.5336486788573609 1105 32-33 0.5100982700941898 1105 34-35 0.6285913149185021 1105 36-37 0.5591813788132924 1105 38-39 0.4632384270076173 1105 40-41 0.6610817671772082 1105 42-43 0.4663993867557963 1105 44-45 0.6247146297241031 1105 46-47 0.575924543072361 1105 48-49 0.5340680581950039 1105 50-51 0.47549944414205214 1105 52-53 0.5028695895438204 1105 54-55 0.5944173304728579 1105 56-57 0.6558978759910019 1105 58-59 0.6767740093058947 1105 60-61 0.5678525609406861 1105 62-63 0.4568525337522047 1105 64-65 0.6650340514966189 1105 66-67 0.6450766961518539 1105 68-69 0.8019878010276642 1105 70-71 0.7799615453564641 1105 72-73 0.7129788466737637 1105 74-75 0.6071970517562342 1105 76-77 0.49687907826751854 1105 78-79 0.5129711716025795 1105 80-81 0.6764063973839072 1105 82-83 0.7895974514087918 1105 84-85 0.7290799996892936 1105 86-87 0.8573735678388879 1105 88-89 0.8507148592326281 1105 90-91 0.8308855744361487 1105 92-93 0.72719902977515 1105 94-95 0.8697020099844934 1105 96-97 0.9651564457670005 1105 98-99 1.0172019673535608 1105 100-101 1.3760084490958704 1106 1 0.5989077021726814 1106 2 0.671475692054802 1106 3 0.7051718644351403 1106 4 0.5612126650173437 1106 5 0.5398796881362742 1106 6 0.6143036703545093 1106 7 0.6083333071284827 1106 8 0.49494315448776405 1106 9 0.6287356996936779 1106 10-11 0.756149261345513 1106 12-13 0.6856548713774018 1106 14-15 0.9616142522567728 1106 16-17 0.8667241496657354 1106 18-19 0.9393263082523049 1106 20-21 0.9691813117907415 1106 22-23 1.039304430619616 1106 24-25 1.0501496563819046 1106 26-27 1.0726015969919303 1106 28-29 0.9505546481725347 1106 30-31 0.9558654593714664 1106 32-33 1.065458435792337 1106 34-35 1.0726132011655451 1106 36-37 1.117207919397245 1106 38-39 1.082480942727905 1106 40-41 0.9886210597993568 1106 42-43 1.1596499276419578 1106 44-45 1.3371508427095264 1106 46-47 1.113322415113629 1106 48-49 1.2122685630593466 1106 50-51 1.2308066556372026 1106 52-53 1.2004552668289676 1106 54-55 1.2279769280924384 1106 56-57 1.2795310752586246 1106 58-59 1.5252533474431758 1106 60-61 1.389838815200882 1106 62-63 1.3563784718116594 1106 64-65 1.4655751947217581 1106 66-67 1.5222660864453346 1106 68-69 1.4648063281587085 1106 70-71 1.3758349175288416 1106 72-73 1.4357160939090932 1106 74-75 1.4839098765757335 1106 76-77 1.4223370404762363 1106 78-79 1.504675869997211 1106 80-81 1.6193877525420959 1106 82-83 1.5909368194498903 1106 84-85 1.652408505524651 1106 86-87 1.6552644303218038 1106 88-89 1.5618475583523868 1106 90-91 1.6218537198789242 1106 92-93 1.6144066891569047 1106 94-95 1.6719465618374798 1106 96-97 1.7673745093419981 1106 98-99 1.9382741636699201 1106 100-101 2.0970409465247215 1107 1 0.31356859186872654 1107 2 0.2802251624965173 1107 3 0.46733383469219447 1107 4 0.43997562638829635 1107 5 0.40811152815891916 1107 6 0.41728558984068087 1107 7 0.476595884167601 1107 8 0.5191076914048267 1107 9 0.5927543287083736 1107 10-11 0.6456144229626517 1107 12-13 0.6344500994675073 1107 14-15 0.5660176515325261 1107 16-17 0.691016590694737 1107 18-19 0.7042046921238097 1107 20-21 0.7448165792051711 1107 22-23 0.905563740480666 1107 24-25 0.8154373905967773 1107 26-27 0.706027530551367 1107 28-29 0.6573992813703242 1107 30-31 0.7825562216351827 1107 32-33 0.8321996258247779 1107 34-35 0.8792547317831847 1107 36-37 0.8701616513714114 1107 38-39 0.7554238381167337 1107 40-41 0.9294686246890151 1107 42-43 0.9268956574602498 1107 44-45 0.974245322579641 1107 46-47 0.9709954943002828 1107 48-49 1.0044330780554347 1107 50-51 0.771864638040384 1107 52-53 0.7984031778317231 1107 54-55 0.953029907748352 1107 56-57 0.9625558311852771 1107 58-59 0.8934635047642772 1107 60-61 0.9598059146796842 1107 62-63 0.8291986097517636 1107 64-65 0.9838649108785162 1107 66-67 1.1055169381466143 1107 68-69 0.9901515000882313 1107 70-71 1.0729726103083195 1107 72-73 0.8952863141719831 1107 74-75 1.000077547238842 1107 76-77 0.918962640265029 1107 78-79 0.9897557886227446 1107 80-81 0.8787474123285897 1107 82-83 0.92767251017737 1107 84-85 0.9511808833831346 1107 86-87 0.9724945057061269 1107 88-89 1.0156647145636235 1107 90-91 0.9499761361229382 1107 92-93 1.0812334662925664 1107 94-95 0.9223696127889696 1107 96-97 0.9299390153790839 1107 98-99 0.9278285817705445 1107 100-101 1.1758125333220057 1108 1 0.15328996121046856 1108 2 0.2836139080281477 1108 3 0.29254297903764837 1108 4 0.17080422004726614 1108 5 0.20593993015776846 1108 6 0.1532460630084742 1108 7 0.18925378693203498 1108 8 -0.0666602119984745 1108 9 0.07554532313669426 1108 10-11 0.12189785823970567 1108 12-13 0.014584253089225285 1108 14-15 0.33067721788986404 1108 16-17 0.3060706257325805 1108 18-19 0.5119460051183253 1108 20-21 0.4155764375374531 1108 22-23 0.36014949442206756 1108 24-25 0.5875921157379693 1108 26-27 0.4683982816094101 1108 28-29 0.4354522091796156 1108 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88-89 -2.601508439647649 2316 90-91 -2.5055169114928084 2316 92-93 -2.943056750998309 2316 94-95 -2.9880458989965923 2316 96-97 -2.871979386496445 2316 98-99 -3.0199715140845846 2316 100-101 -2.6960274232544634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7106.0 3 2278.0 4 2412.0 5 2463.0 6 2740.0 7 3317.0 8 3847.0 9 4706.0 10 5847.0 11 7143.0 12 8539.0 13 10283.0 14 11088.0 15 12951.0 16 15359.0 17 19276.0 18 17317.0 19 13133.0 20 13435.0 21 13877.0 22 15565.0 23 17230.0 24 19608.0 25 22553.0 26 26401.0 27 31177.0 28 36835.0 29 44815.0 30 53927.0 31 67034.0 32 85040.0 33 109722.0 34 148006.0 35 208446.0 36 307993.0 37 443319.0 38 391044.0 39 70188.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64533189137861 18.937261780726164 14.283352896438636 23.134053431456593 2 15.324467876903942 20.243261298751104 40.32370922463979 24.10856159970516 3 19.18594426525882 24.739677676910144 29.528799448491267 26.54557860933977 4 12.812586062627638 16.658777134199187 37.308308557561766 33.22032824561141 5 13.891086813959655 37.322820238660206 34.056119587844705 14.729973359535434 6 31.650303448524586 36.206715318707815 17.675002429963182 14.467978802804419 7 28.23289920547961 32.69920081708524 20.981135693948442 18.086764283486705 8 25.929856427555176 35.068047929315476 19.918988989627337 19.08310665350201 9 26.13984677700318 17.057627542132806 20.496743043556293 36.30578263730772 10-11 24.61248024519056 26.836301081549703 27.55627124205574 20.994947431204 12-13 25.704510047309263 24.194219759946435 27.930387324679206 22.170882868065092 14-15 22.679607602719305 25.583336045365108 26.19708349568875 25.53997285622684 16-17 22.075424019008967 28.825375139627884 26.69012143859551 22.409079402767638 18-19 22.00254758470895 27.51099038949725 28.86270989923342 21.623752126560383 20-21 22.84567182114757 26.68021516075609 28.855808129052484 21.618304889043856 22-23 22.452683803217738 26.663912706346416 28.76858181229789 22.114821678137957 24-25 22.34206136167442 26.743189418651454 28.878289387634066 22.036459832040062 26-27 22.188680207441756 26.88346325918482 28.786236256236375 22.14162027713705 28-29 22.0413629486852 27.02175162269928 28.924892204756226 22.011993223859296 30-31 22.257301057288398 26.782734531464573 28.862276449353725 22.097687961893307 32-33 22.046066913668 26.955103034810747 28.829680074148754 22.1691499773725 34-35 22.401744536674478 26.89077920411009 28.62566747604823 22.081808783167208 36-37 22.481605477606994 26.713840224154257 28.634857850717978 22.169696447520774 38-39 22.18919915721582 26.962236041991506 28.70398414658431 22.144580654208358 40-41 22.40474097366441 26.90776904240747 28.640722594873225 22.046767389054896 42-43 22.284061085502866 26.952480040553084 28.427167665509145 22.3362912084349 44-45 22.285517727955583 27.04805884298756 28.36910281587829 22.29732061317857 46-47 22.481209947519712 27.169756586407868 28.01831975175154 22.330713714320876 48-49 22.554252805910572 27.26384405627404 27.84037799105001 22.34152514676538 50-51 22.44862576094215 27.393324167125016 27.774402282864912 22.383647789067922 52-53 22.519942436379363 27.459750299773894 27.617307415052338 22.4029998487944 54-55 22.439043474094493 27.293850697666443 27.6352441667718 22.631861661467266 56-57 22.406085405897038 27.639006083779392 27.553680632683164 22.4012278776404 58-59 22.63401297381563 27.399233775091954 27.563829911171283 22.402923339921134 60-61 22.580881982829318 27.21141382820394 27.909842380446726 22.297861808520015 62-63 22.36790446625196 27.31902220530066 27.94395983024312 22.369113498204257 64-65 22.39256873297829 27.44295035331309 27.78832950335731 22.37615141035131 66-67 22.34913999664106 27.518436808372382 27.53041732651111 22.60200586847545 68-69 22.16371812616619 27.45530920825165 27.841963768197175 22.53900889738499 70-71 22.3396473349944 27.32117732549516 27.761332890636144 22.577842448874296 72-73 22.358909207244295 27.312951208407966 27.631598607109648 22.69654097723809 74-75 22.25594132553726 27.67859575780962 27.442432627473597 22.62303028917952 76-77 22.426057129695543 27.488152283730688 27.47571281351146 22.610077773062308 78-79 22.424255613713797 27.469975817104235 27.415415370526325 22.69035319865564 80-81 22.33350614917443 27.652197534000727 27.37611708518649 22.638179231638354 82-83 22.384707079939165 27.590465181046863 27.483922417876677 22.5409053211373 84-85 22.472980096521166 27.51389629580128 27.312271958081837 22.70085164959572 86-87 22.37768771140677 27.49165694106671 27.451274359522802 22.679380988003714 88-89 22.537206610061975 27.393585851978326 27.290835470759678 22.778372067200024 90-91 22.4443744913809 27.42199415178711 27.461562574602965 22.67206878222903 92-93 22.325902475486963 27.438354658576262 27.466539154904808 22.769203711031967 94-95 22.295691680972617 27.41656963245434 27.423297533278923 22.86444115329412 96-97 22.203857027952083 27.458533189499928 27.432916711926108 22.904693070621878 98-99 22.199928999923895 27.47102479855818 27.283780793643604 23.04526540787432 100-101 22.291735764765292 27.375625621882886 27.347187263838464 22.985451349513358 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 461.0 1 350.5 2 304.5 3 381.0 4 373.5 5 387.5 6 580.5 7 1116.0 8 1850.5 9 2204.5 10 2069.0 11 1944.0 12 2011.5 13 2166.5 14 2357.0 15 2505.5 16 2609.0 17 2791.0 18 3003.0 19 3285.5 20 3725.5 21 4341.0 22 5269.5 23 6510.0 24 8198.5 25 10545.0 26 13508.5 27 16785.0 28 19735.0 29 22782.5 30 26758.5 31 30777.0 32 34644.0 33 38771.5 34 44243.5 35 48981.5 36 53351.5 37 58243.5 38 61583.0 39 64508.5 40 67098.5 41 70398.0 42 74040.0 43 78288.5 44 82629.5 45 85580.0 46 87521.0 47 89010.5 48 92543.0 49 91480.0 50 85014.0 51 80423.0 52 77520.0 53 75409.5 54 72856.5 55 68094.0 56 62471.5 57 56708.5 58 51620.0 59 46647.5 60 39739.0 61 32171.5 62 25419.5 63 19945.5 64 15366.0 65 11712.0 66 8794.0 67 6781.0 68 4829.0 69 3144.0 70 2212.5 71 1499.5 72 1011.0 73 727.0 74 524.0 75 377.5 76 254.5 77 139.0 78 76.0 79 51.5 80 33.0 81 23.5 82 19.0 83 16.0 84 14.0 85 10.5 86 8.5 87 9.0 88 5.0 89 3.0 90 2.0 91 1.5 92 4.0 93 3.0 94 1.0 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06054300662370368 2 0.10100752701588882 3 0.06941796115054559 4 0.13242135120743317 5 0.10803719396784278 6 0.10417087714426809 7 0.1151547317566962 8 0.10118326868968765 9 0.12214046329020047 10-11 0.11295796083421057 12-13 0.09667988829859213 14-15 0.0988546915118529 16-17 0.11486915153677306 18-19 0.0944391819576568 20-21 0.07769978752831637 22-23 0.07765585210986665 24-25 0.06755070586643282 26-27 0.05751146275067353 28-29 0.07104357163318495 30-31 0.05237101879205718 32-33 0.0519096968983352 34-35 0.04745025192568939 36-37 0.05298611465035315 38-39 0.053908758437797105 40-41 0.07023076639186526 42-43 0.06671593291588827 44-45 0.05274446984887973 46-47 0.04077206832133311 48-49 0.048043380074760514 50-51 0.029941987673479 52-53 0.04481412681870665 54-55 0.04951521659282588 56-57 0.05485336993446593 58-59 0.06192697230486963 60-61 0.0579288492259458 62-63 0.06682577146201256 64-65 0.0453413518401032 66-67 0.06774841524945652 68-69 0.05705014085695155 70-71 0.02734979798494597 72-73 0.02249493424625275 74-75 0.0322485971420889 76-77 0.046198092499872584 78-79 0.06706741626348597 80-81 0.04422099866963553 82-83 0.06126794102812394 84-85 0.0677044798310068 86-87 0.06908844551217275 88-89 0.09272570063811802 90-91 0.06708938397271083 92-93 0.07767781981909151 94-95 0.08593767848763745 96-97 0.09406573090083425 98-99 0.1243811696311358 100-101 0.11994369236771485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.63644502415547 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80126683847473 46.39790972622348 2 12.878311882150511 15.360334771275944 3 4.044346663842348 7.235713724305821 4 1.806891408196511 4.3102632051812035 5 0.9213580432727906 2.747325914760062 6 0.5590149923914138 2.0002600116857545 7 0.3645701063074586 1.5219165571578688 8 0.2813272692859434 1.3421886582853557 9 0.1875736302699519 1.0067602040617727 >10 1.058626893100536 11.446033104683826 >50 0.0697071166809474 2.8255644881844972 >100 0.025675264134493333 2.5524109377557145 >500 9.604774779170596E-4 0.38068811872873454 >1k 2.2164864874978305E-4 0.28996626265638176 >5k 1.4776576583318873E-4 0.582664315053638 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7678 0.3373361428568918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5546 0.243665830722105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3579 0.15724486263152065 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.39354184497124E-5 0.0 16-17 0.0 0.0 0.0 4.39354184497124E-5 0.0 18-19 0.0 0.0 0.0 8.78708368994248E-5 0.0 20-21 0.0 0.0 0.0 8.78708368994248E-5 0.0 22-23 0.0 0.0 0.0 1.9770938302370578E-4 0.0 24-25 0.0 0.0 0.0 3.514833475976992E-4 0.0 26-27 0.0 0.0 0.0 6.370635675208298E-4 4.39354184497124E-5 28-29 0.0 0.0 0.0 0.0015816750641896465 4.39354184497124E-5 30-31 0.0 0.0 0.0 0.0033390918021781422 4.39354184497124E-5 32-33 0.0 0.0 0.0 0.005733572107687468 6.59031276745686E-5 34-35 0.0 0.0 0.0 0.008874954526841904 8.78708368994248E-5 36-37 0.0 0.0 0.0 0.011533047343049503 8.78708368994248E-5 38-39 0.0 0.0 0.0 0.014476720379180234 8.78708368994248E-5 40-41 0.0 0.0 0.0 0.018826326805701763 8.78708368994248E-5 42-43 0.0 0.0 0.0 0.024120544728892107 8.78708368994248E-5 44-45 0.0 0.0 0.0 0.029260988687508458 8.78708368994248E-5 46-47 0.0 0.0 0.0 0.03574146290884103 8.78708368994248E-5 48-49 0.0 0.0 0.0 0.04184848607335106 8.78708368994248E-5 50-51 0.0 0.0 0.0 0.05034998954337041 8.78708368994248E-5 52-53 0.0 0.0 0.0 0.06994518617194215 1.318062553491372E-4 54-55 0.0 0.0 0.0 0.09415360173773367 1.318062553491372E-4 56-57 0.0 2.19677092248562E-5 0.0 0.11717576100538296 1.5377396457399338E-4 58-59 0.0 4.39354184497124E-5 0.0 0.15526776880128362 1.757416737988496E-4 60-61 0.0 4.39354184497124E-5 0.0 0.20199308632255275 1.757416737988496E-4 62-63 0.0 4.39354184497124E-5 0.0 0.23369249073402026 1.757416737988496E-4 64-65 0.0 4.39354184497124E-5 0.0 0.25972422616547486 1.757416737988496E-4 66-67 0.0 4.39354184497124E-5 0.0 0.32993302484811526 1.757416737988496E-4 68-69 0.0 4.39354184497124E-5 0.0 0.40596326647534253 1.757416737988496E-4 70-71 0.0 4.39354184497124E-5 0.0 0.4384095730004552 1.757416737988496E-4 72-73 0.0 4.39354184497124E-5 0.0 0.4976784524891172 1.757416737988496E-4 74-75 0.0 4.39354184497124E-5 0.0 0.5888224780630456 1.757416737988496E-4 76-77 0.0 4.39354184497124E-5 0.0 0.6823829516517081 1.757416737988496E-4 78-79 0.0 4.39354184497124E-5 0.0 0.7654428602308894 1.757416737988496E-4 80-81 0.0 4.39354184497124E-5 0.0 0.879762819037041 1.757416737988496E-4 82-83 0.0 4.39354184497124E-5 0.0 1.0115251389677287 1.757416737988496E-4 84-85 0.0 4.39354184497124E-5 0.0 1.1553037958444123 1.757416737988496E-4 86-87 0.0 4.39354184497124E-5 0.0 1.3720811504752932 1.757416737988496E-4 88-89 0.0 4.39354184497124E-5 0.0 1.634727081967674 1.757416737988496E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10390 0.0 32.436592 1 GGTATCA 6775 0.0 30.94096 1 TCAACGC 12410 0.0 26.887516 4 TATCAAC 12560 0.0 26.832497 2 ATCAACG 12575 0.0 26.610315 3 CAACGCA 12605 0.0 26.471567 5 AACGCAG 13045 0.0 25.68744 6 ACGCAGA 14610 0.0 22.708113 7 CGCAGAG 14640 0.0 22.433819 8 GTACATG 8940 0.0 22.278246 1 TACATGG 8780 0.0 22.196978 2 GAGTACT 6790 0.0 21.452091 12-13 GCAGAGT 15040 0.0 21.141924 9 ACATGGG 8790 0.0 20.764797 3 GTACTTT 7175 0.0 20.167202 14-15 AGTACTT 7300 0.0 19.953382 12-13 GTATAGG 915 0.0 19.740902 1 AGAGTAC 12155 0.0 19.432108 10-11 CATGGGG 4235 0.0 17.508808 4 GTCCTAC 645 0.0 16.950113 1 >>END_MODULE