FastQCFastQC Report
Thu 26 May 2016
SRR936859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276068
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91230.40082282251672624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71310.3133034689648991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.2082978188700865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110100.030.8510741
GGTATCA74900.030.1275181
ATCAACG135750.024.8779473
TCAACGC136800.024.7211764
TATCAAC137900.024.4916882
CAACGCA139100.024.3807095
AACGCAG142050.023.8409486
GTACATG90700.021.6318821
ACGCAGA160800.020.795147
GAGTACT83100.020.7196412-13
CGCAGAG160650.020.6667238
TACATGG91400.020.6327782
AGTACTT88950.020.13124512-13
ACATGGG89450.020.0722583
AGAGTAC136300.019.5672210-11
GCAGAGT169900.019.2619869
GTACTTT88350.019.08542414-15
GTATAGA7400.017.9753721
TACTTTT102400.017.41766714-15
AGTACAT70450.016.3847062
Produced by FastQC (version 0.11.5)