##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936859_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276068 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.110286248038285 31.0 31.0 34.0 31.0 34.0 2 32.10230450056852 33.0 31.0 34.0 30.0 34.0 3 32.28885736278529 33.0 31.0 34.0 30.0 34.0 4 36.02557085289192 37.0 35.0 37.0 35.0 37.0 5 35.94981345021326 37.0 35.0 37.0 35.0 37.0 6 35.99060265334779 37.0 35.0 37.0 35.0 37.0 7 35.92935755873726 37.0 35.0 37.0 35.0 37.0 8 35.90675058917396 37.0 35.0 37.0 35.0 37.0 9 37.588983721048756 39.0 37.0 39.0 35.0 39.0 10-11 37.51707835618268 39.0 37.0 39.0 35.0 39.0 12-13 37.41946725669005 39.0 37.0 39.0 34.0 39.0 14-15 38.63012704365599 40.0 38.0 41.0 34.0 41.0 16-17 38.543674442064116 40.0 38.0 41.0 34.0 41.0 18-19 38.59947440058909 40.0 38.0 41.0 34.0 41.0 20-21 38.68291676698587 40.0 38.0 41.0 34.0 41.0 22-23 38.613480572636675 40.0 38.0 41.0 34.0 41.0 24-25 38.515567856496375 40.0 38.0 41.0 34.0 41.0 26-27 38.36377054639844 40.0 38.0 41.0 34.0 41.0 28-29 38.19348565157104 40.0 38.0 41.0 34.0 41.0 30-31 38.070436823504394 40.0 37.0 41.0 33.5 41.0 32-33 38.06474520972132 40.0 37.0 41.0 33.0 41.0 34-35 37.982229001945456 40.0 37.0 41.0 33.0 41.0 36-37 37.831496027359464 40.0 37.0 41.0 33.0 41.0 38-39 37.67639894765885 40.0 37.0 41.0 33.0 41.0 40-41 37.499732213624554 40.0 37.0 41.0 33.0 41.0 42-43 37.28799952374007 40.0 37.0 41.0 32.0 41.0 44-45 36.97643370057485 40.0 36.0 41.0 31.0 41.0 46-47 36.723648414722234 39.0 36.0 41.0 31.0 41.0 48-49 36.42007005063118 39.0 36.0 41.0 30.0 41.0 50-51 36.16504779294819 39.0 35.0 40.5 30.0 41.0 52-53 35.905155294130054 39.0 35.0 40.0 29.0 41.0 54-55 35.671186888968165 39.0 35.0 40.0 28.0 41.0 56-57 35.39999178407675 38.0 35.0 40.0 28.0 41.0 58-59 35.07374054729472 38.0 34.0 40.0 27.0 41.0 60-61 34.748996295365515 38.0 34.0 40.0 26.5 41.0 62-63 34.381722997731174 37.0 34.0 40.0 26.0 41.0 64-65 33.98169320951746 37.0 33.0 39.0 26.0 41.0 66-67 33.599116766282904 36.0 33.0 39.0 25.5 40.0 68-69 33.15665349189919 35.5 32.0 39.0 24.5 40.0 70-71 32.680119618570274 35.0 32.0 38.0 23.0 40.0 72-73 32.33145912160796 35.0 31.5 37.5 22.5 39.5 74-75 31.882462650500777 35.0 31.0 37.0 21.5 39.0 76-77 30.492216181590358 33.5 29.5 35.5 20.0 38.0 78-79 31.20764735499994 34.0 31.0 36.0 20.5 38.0 80-81 31.038522355219616 34.0 31.0 36.0 20.0 37.0 82-83 30.707297848746173 34.0 31.0 35.0 18.5 37.0 84-85 30.322824054465862 34.0 30.0 35.0 18.0 36.5 86-87 29.95427113776917 34.0 30.0 35.0 13.5 36.0 88-89 29.587684331048106 34.0 30.0 35.0 7.5 36.0 90-91 29.160062880370887 34.0 29.0 35.0 4.5 35.0 92-93 28.69573536467276 34.0 29.0 35.0 2.0 35.0 94-95 28.215975313567082 33.5 29.0 35.0 2.0 35.0 96-97 27.56896696408016 33.0 27.0 35.0 2.0 35.0 98-99 26.798840807919625 33.0 26.0 35.0 2.0 35.0 100-101 23.68255429978366 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3789394565108921 1101 2 0.2336167729218772 1101 3 0.25402657066416623 1101 4 0.2419618654930602 1101 5 0.24438145705831715 1101 6 0.238979554383981 1101 7 0.2794128381224965 1101 8 0.24432901868250667 1101 9 0.3719681613748662 1101 10-11 0.3768110105715863 1101 12-13 0.34945212400757697 1101 14-15 0.5122539200803047 1101 16-17 0.5014357912208993 1101 18-19 0.44955255890395307 1101 20-21 0.5107572634335753 1101 22-23 0.38403754703717397 1101 24-25 0.42148472282036664 1101 26-27 0.43497989987627506 1101 28-29 0.5296571862064781 1101 30-31 0.5643999147350698 1101 32-33 0.5879941527566572 1101 34-35 0.5522777926867306 1101 36-37 0.4351356775367705 1101 38-39 0.46205674232439264 1101 40-41 0.3667403773257192 1101 42-43 0.5063237994926695 1101 44-45 0.6003549487855011 1101 46-47 0.5395782748344189 1101 48-49 0.3563955423496097 1101 50-51 0.34930951946253685 1101 52-53 0.260823925990735 1101 54-55 0.27142346435278597 1101 56-57 0.27042608496542186 1101 58-59 0.3195187892395879 1101 60-61 0.3552419447448898 1101 62-63 0.4370630400239506 1101 64-65 0.38569508985502665 1101 66-67 0.405076084878381 1101 68-69 0.33691546364435254 1101 70-71 0.40841699629710604 1101 72-73 0.6252186486905984 1101 74-75 0.5228773176014059 1101 76-77 0.45001268804877625 1101 78-79 0.5202831829873276 1101 80-81 0.5515005754101772 1101 82-83 0.7143607751962904 1101 84-85 0.6105275314664915 1101 86-87 0.555306160376194 1101 88-89 0.4390398070486192 1101 90-91 0.5870594285200248 1101 92-93 0.6356334492135467 1101 94-95 0.7177815967009913 1101 96-97 0.7420848065028594 1101 98-99 0.7611848717710039 1101 100-101 0.7975646479651424 1102 1 0.23325036349621087 1102 2 0.13336556076991712 1102 3 0.18598896599326764 1102 4 0.13907652417367444 1102 5 0.17750312440047367 1102 6 0.2129498606730209 1102 7 0.28796294269621114 1102 8 0.2955213942411845 1102 9 0.3390177233933329 1102 10-11 0.3144931124683126 1102 12-13 0.36021650790184623 1102 14-15 0.4206671448321444 1102 16-17 0.4114043144439137 1102 18-19 0.40480356272585993 1102 20-21 0.41779431368737363 1102 22-23 0.34006535281933736 1102 24-25 0.38449457573535994 1102 26-27 0.3142948987030536 1102 28-29 0.37328385157598376 1102 30-31 0.37081464624149874 1102 32-33 0.4332623393222974 1102 34-35 0.3793601612060442 1102 36-37 0.4721574230076868 1102 38-39 0.4525079493328974 1102 40-41 0.48370932857229576 1102 42-43 0.572537868290226 1102 44-45 0.6354601574554621 1102 46-47 0.6029861677324462 1102 48-49 0.5158066082438992 1102 50-51 0.5739529112432464 1102 52-53 0.5392052074657698 1102 54-55 0.6018974261863193 1102 56-57 0.6005084062366777 1102 58-59 0.5975905660198251 1102 60-61 0.6515655273678931 1102 62-63 0.7735278365313363 1102 64-65 0.6093641150039488 1102 66-67 0.6623497442445867 1102 68-69 0.5187468303353384 1102 70-71 0.504028429051651 1102 72-73 0.6689491202401925 1102 74-75 0.5157302012980125 1102 76-77 0.44181325578305675 1102 78-79 0.6364106403100678 1102 80-81 0.5808116169627517 1102 82-83 0.6063118389654178 1102 84-85 0.6389375704630851 1102 86-87 0.46048787701183613 1102 88-89 0.6959487393042743 1102 90-91 0.6330435527732483 1102 92-93 0.6955081042977262 1102 94-95 0.7854744101514974 1102 96-97 0.937972869669327 1102 98-99 1.087935307719082 1102 100-101 1.2092203455727777 1103 1 0.1446416472485197 1103 2 -0.11646731281720335 1103 3 0.015520587887074555 1103 4 0.14156831236435607 1103 5 0.1921674583665336 1103 6 0.2029742624430355 1103 7 0.22166865232002664 1103 8 0.2277930635271943 1103 9 0.2513967514589339 1103 10-11 0.3080058675636437 1103 12-13 0.2960034621698284 1103 14-15 0.4100407764318632 1103 16-17 0.4662155845258269 1103 18-19 0.5191103343765633 1103 20-21 0.40169697160729356 1103 22-23 0.49358696251788814 1103 24-25 0.4793871888042105 1103 26-27 0.49759569509184587 1103 28-29 0.4978891198209112 1103 30-31 0.48542723764491313 1103 32-33 0.517981261035068 1103 34-35 0.43175311866228583 1103 36-37 0.38285036045755305 1103 38-39 0.47530029481180947 1103 40-41 0.45268330940145773 1103 42-43 0.4298369665770778 1103 44-45 0.5851005869956722 1103 46-47 0.5007625137428633 1103 48-49 0.45947674931323235 1103 50-51 0.28276586891719546 1103 52-53 0.43588336121906934 1103 54-55 0.3458080566273125 1103 56-57 0.5046460658513183 1103 58-59 0.5554337963772582 1103 60-61 0.5639154654907657 1103 62-63 0.3815644117855186 1103 64-65 0.34462949598273696 1103 66-67 0.31838547570274756 1103 68-69 0.4420537299818008 1103 70-71 0.3749048358318916 1103 72-73 0.19805736147726805 1103 74-75 0.2915542818399999 1103 76-77 0.2910391442555955 1103 78-79 0.3764179634685547 1103 80-81 0.4782409481080201 1103 82-83 0.48835474869538587 1103 84-85 0.5343187819634814 1103 86-87 0.3586969124606547 1103 88-89 0.6111811153970699 1103 90-91 0.739327975090152 1103 92-93 0.6892528282266639 1103 94-95 0.670780836101077 1103 96-97 0.9818512008900413 1103 98-99 1.049942528781255 1103 100-101 1.336020027722924 1104 1 0.20439433210729874 1104 2 0.06847786277995738 1104 3 0.15324013300638484 1104 4 0.14912054952110765 1104 5 0.08792303990269801 1104 6 0.04699919789629803 1104 7 0.19079449129783654 1104 8 0.16814364603300191 1104 9 0.19856075523662753 1104 10-11 0.23578879325972224 1104 12-13 0.20292702911730487 1104 14-15 0.24085592488287233 1104 16-17 0.2958948846660263 1104 18-19 0.38492653774913066 1104 20-21 0.2418464145011754 1104 22-23 0.3071892767450919 1104 24-25 0.2815657593984895 1104 26-27 0.24497090565900947 1104 28-29 0.34284707684017945 1104 30-31 0.4206225167875317 1104 32-33 0.3813755944192039 1104 34-35 0.4333509818250576 1104 36-37 0.3426995641493775 1104 38-39 0.3304298091437232 1104 40-41 0.4126133471524511 1104 42-43 0.4060859362209115 1104 44-45 0.4793381197325388 1104 46-47 0.567879533650661 1104 48-49 0.5830222460960002 1104 50-51 0.5488027158994342 1104 52-53 0.4389255769918705 1104 54-55 0.4395901191046292 1104 56-57 0.4771731461881359 1104 58-59 0.5039569618241799 1104 60-61 0.4428130669069219 1104 62-63 0.5715486454359961 1104 64-65 0.5213336763272309 1104 66-67 0.6016885518836617 1104 68-69 0.5516339379478055 1104 70-71 0.5268260537712735 1104 72-73 0.6418800078434614 1104 74-75 0.6172858764252993 1104 76-77 0.5354062525061174 1104 78-79 0.5714450558563229 1104 80-81 0.6343686889110138 1104 82-83 0.6101583271227788 1104 84-85 0.6997049889290814 1104 86-87 0.6427177617151436 1104 88-89 0.7630787876357239 1104 90-91 0.8279904235538034 1104 92-93 0.7800665565196603 1104 94-95 0.9210360381250418 1104 96-97 0.9958912982913759 1104 98-99 0.9246139550166497 1104 100-101 0.698970144580052 1105 1 0.18717752453174796 1105 2 0.024545903025291693 1105 3 0.054352283237669496 1105 4 0.06667634865809902 1105 5 0.1498594210218016 1105 6 0.1594176096857396 1105 7 0.13570317964224188 1105 8 0.13141432973920786 1105 9 0.11567415165134065 1105 10-11 0.20589284919494588 1105 12-13 0.2671932520878997 1105 14-15 0.3552875923968699 1105 16-17 0.29468645573395236 1105 18-19 0.2657074679474434 1105 20-21 0.2486411763464389 1105 22-23 0.34686658142837956 1105 24-25 0.31165561127725994 1105 26-27 0.27085479339370266 1105 28-29 0.24252474670542057 1105 30-31 0.36467160104623275 1105 32-33 0.26388980751010394 1105 34-35 0.31990425949476986 1105 36-37 0.2628550974262893 1105 38-39 0.3000287918076623 1105 40-41 0.08397260193780909 1105 42-43 0.0718796848764498 1105 44-45 0.30285813902631986 1105 46-47 0.23528103390182054 1105 48-49 0.38504750337335025 1105 50-51 0.38286032677637394 1105 52-53 0.45956501439863473 1105 54-55 0.28553059863038044 1105 56-57 0.45241713813663864 1105 58-59 0.2627946028780954 1105 60-61 0.3850012447878157 1105 62-63 0.10459409366165318 1105 64-65 0.22257166217376323 1105 66-67 0.23474991976016213 1105 68-69 0.08434577724188586 1105 70-71 0.016972702855952093 1105 72-73 0.038628804312153875 1105 74-75 -0.04767480089592624 1105 76-77 -0.027032729298117175 1105 78-79 0.04667830156500585 1105 80-81 0.2688326788319948 1105 82-83 0.27875589337524787 1105 84-85 0.2163838188176257 1105 86-87 0.3628573373014383 1105 88-89 0.43969599687338246 1105 90-91 0.5915779977052402 1105 92-93 0.559702926588205 1105 94-95 0.5792436071677329 1105 96-97 0.9193243396122845 1105 98-99 0.9968866021464642 1105 100-101 1.0321690072665035 1106 1 0.3677455449766782 1106 2 0.27425615255828006 1106 3 0.21856105378592616 1106 4 0.2534383790863117 1106 5 0.18683571591300563 1106 6 0.2603267723275664 1106 7 0.3188370983404383 1106 8 0.2803097461941704 1106 9 0.3852810097235704 1106 10-11 0.3662068369951399 1106 12-13 0.4350639991266263 1106 14-15 0.5452675574234078 1106 16-17 0.5076811578548259 1106 18-19 0.5273308484463044 1106 20-21 0.44930821153919 1106 22-23 0.4951678557492585 1106 24-25 0.48449352514542454 1106 26-27 0.5233048473426223 1106 28-29 0.490210822387688 1106 30-31 0.5267615328853026 1106 32-33 0.6175658775527069 1106 34-35 0.6250854337279534 1106 36-37 0.5464666545413905 1106 38-39 0.6362360101651774 1106 40-41 0.6195768710882206 1106 42-43 0.5965073010409654 1106 44-45 0.7592291510595999 1106 46-47 0.5133849291986792 1106 48-49 0.6328831204731031 1106 50-51 0.68258390986027 1106 52-53 0.7791268647512624 1106 54-55 0.643487822632018 1106 56-57 0.6522310719543896 1106 58-59 0.634560602077407 1106 60-61 0.683314466775002 1106 62-63 0.6993566346318758 1106 64-65 0.8682471322624608 1106 66-67 0.9695460393727373 1106 68-69 1.1302544335887745 1106 70-71 0.8782053086073205 1106 72-73 0.9697952698392811 1106 74-75 0.9469705434148068 1106 76-77 0.7927990332542514 1106 78-79 0.8496536840076772 1106 80-81 0.9231493628482959 1106 82-83 0.8600754656197367 1106 84-85 0.8981585647369208 1106 86-87 0.7669107591287165 1106 88-89 1.1278930884381069 1106 90-91 1.3021613724102075 1106 92-93 1.2630409190270875 1106 94-95 1.2471210600996265 1106 96-97 1.5480589353374583 1106 98-99 1.7351448626333088 1106 100-101 1.6345627428893472 1107 1 0.18423078382235758 1107 2 0.12715355716273535 1107 3 0.16832842937368042 1107 4 0.11209551934288697 1107 5 0.17677208314076864 1107 6 0.21830522019877918 1107 7 0.19865441085774194 1107 8 0.15544264209290048 1107 9 0.25908363614205854 1107 10-11 0.20958017451569333 1107 12-13 0.23703266929080513 1107 14-15 0.28184000838356127 1107 16-17 0.31124755719682184 1107 18-19 0.3256614710448318 1107 20-21 0.3000033923085823 1107 22-23 0.343659502190647 1107 24-25 0.33944923763185386 1107 26-27 0.33737544335957637 1107 28-29 0.3654107916983307 1107 30-31 0.3780251101879415 1107 32-33 0.30581652195782993 1107 34-35 0.3085411035637904 1107 36-37 0.4406436339933393 1107 38-39 0.41851309412298576 1107 40-41 0.45358435376468975 1107 42-43 0.4151001699578316 1107 44-45 0.4476673150923389 1107 46-47 0.41650066192864443 1107 48-49 0.46253797951887066 1107 50-51 0.3554581830727912 1107 52-53 0.45931011455457593 1107 54-55 0.36499305960012407 1107 56-57 0.3936287579574156 1107 58-59 0.5186582228417151 1107 60-61 0.4517870562661841 1107 62-63 0.4091636967115946 1107 64-65 0.37200371987225367 1107 66-67 0.31529303703338485 1107 68-69 0.5224093224184685 1107 70-71 0.5123177022012584 1107 72-73 0.516590947785879 1107 74-75 0.6670191079008276 1107 76-77 0.6681248098881163 1107 78-79 0.5892864934138764 1107 80-81 0.48532987062380784 1107 82-83 0.6600885228949487 1107 84-85 0.6782458149748365 1107 86-87 0.8729809863014744 1107 88-89 0.8135648018528379 1107 90-91 0.998393633696768 1107 92-93 1.0755567938621446 1107 94-95 0.9841842311017999 1107 96-97 1.1072714148520681 1107 98-99 1.0588579466425792 1107 100-101 1.0853438443419918 1108 1 0.051994609476800235 1108 2 -0.05195261281229335 1108 3 0.04574062851912686 1108 4 0.05347100436357266 1108 5 0.13629625833588932 1108 6 0.10313652756195779 1108 7 0.15136343991095913 1108 8 0.2137631663531394 1108 9 0.2911778212248066 1108 10-11 0.1375340567754577 1108 12-13 0.23507830724610557 1108 14-15 0.18182991766867218 1108 16-17 0.2087916845065223 1108 18-19 0.21071278560901163 1108 20-21 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-0.7791600607163112 2316 90-91 -0.7894049030688741 2316 92-93 -1.1363936631820515 2316 94-95 -1.0056434317653071 2316 96-97 -0.8743152690800713 2316 98-99 -0.7601395227241987 2316 100-101 -0.9035108311941613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 15.0 5 49.0 6 82.0 7 244.0 8 585.0 9 1166.0 10 1892.0 11 3107.0 12 4761.0 13 6529.0 14 8431.0 15 11284.0 16 14705.0 17 19814.0 18 18226.0 19 12000.0 20 10566.0 21 11081.0 22 11628.0 23 12979.0 24 15117.0 25 18402.0 26 22570.0 27 28147.0 28 35160.0 29 44706.0 30 55365.0 31 70386.0 32 89443.0 33 116211.0 34 157447.0 35 222825.0 36 338914.0 37 506242.0 38 375525.0 39 30456.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.657528263092516 19.601559276362885 14.986393024299208 22.754519436245396 2 15.518777647139459 20.78682638519089 39.85111625462873 23.84327971304093 3 19.472446590885127 24.754659018214284 29.88252279421586 25.890371596684737 4 13.246695388789936 17.094920964367404 37.046486431388956 32.61189721545371 5 13.805605545088998 37.38686450805613 34.21977371144166 14.58775623541321 6 31.84235234261969 36.48967537366057 17.289372042865246 14.378600240854494 7 28.15265462425079 32.770402967225024 21.032694131013777 18.044248277510405 8 26.1454842298209 35.174696010839746 19.72375166295559 18.956068096383763 9 26.085029094034095 16.87757132036477 20.452508448780968 36.58489113682016 10-11 24.750012906032005 26.911504162332623 27.404811149054236 20.93367178258114 12-13 25.825029310577 24.12010603432122 28.107201252342044 21.94766340275973 14-15 22.684142125806435 25.457916898792128 26.411886639590733 25.446054335810704 16-17 21.959097882840055 28.73027080034516 26.952006706302274 22.358624610512514 18-19 21.896270234456967 27.245297592163325 29.393453974134342 21.464978199245365 20-21 22.654602899407802 26.337814334825783 29.5738189947217 21.433763771044717 22-23 22.344038051587212 26.352046599662227 29.447296829444465 21.8566185193061 24-25 22.217789442627517 26.47416588989283 29.56728186861578 21.740762798863873 26-27 22.168273236794867 26.56441500805024 29.435569174855125 21.831742580299764 28-29 21.907845654586627 26.718485966082394 29.637506557597455 21.73616182173353 30-31 22.115639187314795 26.42004701430181 29.602575569920425 21.861738228462965 32-33 21.98078293112694 26.58703160893469 29.550301959601494 21.881883500336876 34-35 22.34433892110779 26.514398769249965 29.340418244994915 21.80084406464733 36-37 22.4375953575889 26.381535805024008 29.30426444080497 21.87660439658212 38-39 22.13629928919344 26.603972045767893 29.362576372821515 21.897152292217157 40-41 22.371086776665237 26.540212103064675 29.28302186892704 21.805679251343054 42-43 22.204110720249275 26.67029726613224 29.04630420278868 22.079287810829804 44-45 22.331963929638672 26.69878076133163 28.893854115339657 22.075401193690038 46-47 22.475549352841277 26.874260373552683 28.545302648497 22.104887625109043 48-49 22.550178729596357 27.080521461610925 28.251753904986852 22.11754590380587 50-51 22.54910230995262 27.088957076778158 28.160741525721594 22.201199087547625 52-53 22.596024150696692 27.248574499064603 28.002668667801384 22.152732682437318 54-55 22.526442188345094 27.05064536382421 27.99833743628633 22.424575011544363 56-57 22.502580117650727 27.368139402361713 27.912808938608357 22.2164715413792 58-59 22.726960183318113 27.12413009681054 27.936438824653397 22.21247089521795 60-61 22.56327206328173 26.992558140352298 28.33589772429707 22.108272072068903 62-63 22.451811633044354 27.003147533377735 28.347571337938938 22.19746949563897 64-65 22.48357472937955 27.09855513855925 28.197666500004832 22.220203632056364 66-67 22.384385517734216 27.221458599737748 27.96877859236538 22.425377290162658 68-69 22.28138614487792 27.13097763335718 28.190040895087492 22.39759532667741 70-71 22.402967929951018 27.011185315706122 28.138569886749693 22.447276867593164 72-73 22.466683772189583 26.9978093800361 27.981984721018883 22.55352212675544 74-75 22.360853721653843 27.328126084660408 27.73850666371419 22.572513529971555 76-77 22.47912446543891 27.25940275024774 27.755877014098328 22.505595770215013 78-79 22.535028100026118 27.20104056759351 27.688416699783026 22.575514632597347 80-81 22.449137856795122 27.352747592659966 27.65163298411744 22.54648156642747 82-83 22.468710924353502 27.308533176569416 27.743170796218337 22.479585102858746 84-85 22.478670425589733 27.232852806000334 27.62674447546613 22.661732292943803 86-87 22.470141069824905 27.117760879001914 27.72552806721862 22.68656998395456 88-89 22.583189187218014 27.103372645643958 27.59960580778868 22.713832359349347 90-91 22.506125698408702 27.14269834763212 27.70970713955257 22.641468814406608 92-93 22.390219094996084 27.041179578373665 27.725473871051715 22.843127455578536 94-95 22.33031804424035 27.058078694519978 27.715264972428315 22.896338288811357 96-97 22.279917497001918 27.088052333033907 27.69254562258064 22.939484547383536 98-99 22.25080277070953 27.11714092582963 27.489171355216673 23.142884948244163 100-101 22.086263562090533 27.213831624349012 27.532716082334403 23.16718873122605 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 566.0 1 427.0 2 320.5 3 374.5 4 324.5 5 308.5 6 543.0 7 1193.5 8 2053.0 9 2541.5 10 2382.0 11 2100.5 12 2180.0 13 2405.0 14 2594.5 15 2797.0 16 3026.5 17 3152.0 18 3365.5 19 3666.0 20 4086.0 21 4750.5 22 5629.0 23 6915.5 24 8669.5 25 10829.0 26 13662.5 27 17092.5 28 20026.5 29 22935.0 30 27010.5 31 30973.0 32 34564.0 33 38568.5 34 43943.5 35 48632.0 36 53265.0 37 58218.0 38 61318.0 39 64141.5 40 67272.5 41 70910.5 42 74314.0 43 78222.0 44 82409.0 45 85515.0 46 86925.5 47 88445.5 48 92887.0 49 91292.0 50 84325.5 51 79676.5 52 77036.5 53 75590.0 54 72760.5 55 67979.0 56 62100.0 57 56362.5 58 51586.5 59 46429.5 60 39569.0 61 32083.5 62 25181.5 63 19672.0 64 15105.0 65 11336.5 66 8567.0 67 6732.5 68 4809.5 69 3097.5 70 2112.0 71 1435.0 72 979.0 73 673.5 74 495.5 75 368.5 76 230.5 77 110.5 78 67.0 79 43.5 80 26.0 81 14.5 82 10.0 83 11.5 84 11.0 85 6.5 86 3.5 87 3.0 88 2.5 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017442361124535823 2 0.0028118667807815936 3 0.002284641759385045 4 2.636125106982744E-4 5 0.0015816750641896463 6 2.1967709224856202E-4 7 8.78708368994248E-5 8 0.0 9 0.0 10-11 2.19677092248562E-5 12-13 5.49192730621405E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002262674050160188 22-23 0.0 24-25 0.0041518970434978215 26-27 0.0021748032132607635 28-29 0.003602704312876417 30-31 0.01658562046476643 32-33 8.128052413196793E-4 34-35 0.002526286560858463 36-37 0.004195832461947534 38-39 0.0027239959438821685 40-41 0.004349606426521527 42-43 0.0019111907025624892 44-45 7.249344044202547E-4 46-47 1.5377396457399338E-4 48-49 1.757416737988496E-4 50-51 1.757416737988496E-4 52-53 0.0011423208796925224 54-55 0.0028558021992313057 56-57 3.9541876604741157E-4 58-59 0.0010764177520179539 60-61 0.0010544500427930976 62-63 0.0 64-65 8.78708368994248E-5 66-67 6.59031276745686E-5 68-69 0.0 70-71 1.5377396457399338E-4 72-73 0.0 74-75 4.3935418449712405E-4 76-77 8.128052413196793E-4 78-79 1.0983854612428101E-4 80-81 0.004920766866367788 82-83 0.0014938042272902216 84-85 0.0027020282346573127 86-87 0.0014059333903907968 88-89 9.226437874439604E-4 90-91 4.39354184497124E-5 92-93 2.19677092248562E-5 94-95 4.39354184497124E-5 96-97 0.0012960948442665157 98-99 0.0019551261210122017 100-101 3.734510568225554E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.42781335263406 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43801230995683 43.89667903982545 2 13.478800322139936 15.48116058234552 3 4.39599725897014 7.573575302604843 4 1.8718120098354336 4.299762829281924 5 0.9988680141764877 2.8681402941021785 6 0.6032465106069971 2.0785876810059842 7 0.41758981675535334 1.6786889038210966 8 0.30139319774467804 1.3846681844667919 9 0.22069406070590442 1.1406579593628212 >10 1.167076930589163 12.099752382204489 >50 0.07475535989821469 2.94613557940325 >100 0.029992359591454628 2.9174003498099683 >500 0.0013022362390679538 0.48631972255456685 >1k 3.064085268393736E-4 0.43344262562897495 >5k 1.532042634196868E-4 0.7150285635819912 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9123 0.40082282251672624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7131 0.3133034689648991 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.2082978188700865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.39354184497124E-5 0.0 8 0.0 0.0 0.0 4.39354184497124E-5 0.0 9 0.0 0.0 0.0 4.39354184497124E-5 0.0 10-11 0.0 0.0 0.0 4.39354184497124E-5 0.0 12-13 0.0 0.0 0.0 4.39354184497124E-5 0.0 14-15 0.0 0.0 0.0 8.78708368994248E-5 0.0 16-17 0.0 0.0 0.0 8.78708368994248E-5 0.0 18-19 0.0 0.0 0.0 8.78708368994248E-5 0.0 20-21 0.0 0.0 0.0 1.09838546124281E-4 0.0 22-23 0.0 0.0 0.0 2.1967709224856197E-4 0.0 24-25 0.0 0.0 0.0 4.613218937219802E-4 0.0 26-27 0.0 0.0 0.0 7.68869822869967E-4 0.0 28-29 0.0 0.0 0.0 0.0018013521564382083 0.0 30-31 0.0 0.0 0.0 0.003646639731326129 0.0 32-33 0.0 0.0 0.0 0.00612899087373488 4.39354184497124E-5 34-35 0.0 0.0 0.0 0.009336276420563883 4.39354184497124E-5 36-37 0.0 0.0 0.0 0.011994369236771486 4.39354184497124E-5 38-39 0.0 0.0 0.0 0.015113783946701063 4.39354184497124E-5 40-41 0.0 0.0 0.0 0.01959519662857173 4.39354184497124E-5 42-43 0.0 0.0 0.0 0.024823511424087504 4.39354184497124E-5 44-45 0.0 0.0 0.0 0.029963955382703855 4.39354184497124E-5 46-47 0.0 0.0 0.0 0.03644442960403643 4.39354184497124E-5 48-49 0.0 0.0 0.0 0.0425294850593216 4.39354184497124E-5 50-51 0.0 0.0 0.0 0.05081131143709239 4.39354184497124E-5 52-53 0.0 0.0 0.0 0.07045044348411383 4.39354184497124E-5 54-55 0.0 0.0 0.0 0.09450508508533137 4.39354184497124E-5 56-57 0.0 0.0 0.0 0.11770298602677952 4.39354184497124E-5 58-59 0.0 0.0 0.0 0.1558828646595796 4.39354184497124E-5 60-61 0.0 0.0 0.0 0.202718020726973 4.39354184497124E-5 62-63 0.0 0.0 0.0 0.23443939284766535 4.39354184497124E-5 64-65 0.0 0.0 0.0 0.26036128973299566 4.39354184497124E-5 66-67 0.0 0.0 0.0 0.3307458300894349 4.39354184497124E-5 68-69 0.0 0.0 0.0 0.4064465560782894 4.39354184497124E-5 70-71 0.0 0.0 0.0 0.438585314674254 4.39354184497124E-5 72-73 0.0 0.0 0.0 0.4982276452197386 6.59031276745686E-5 74-75 0.0 0.0 0.0 0.5900526697796376 8.78708368994248E-5 76-77 0.0 0.0 0.0 0.6838767558789983 8.78708368994248E-5 78-79 0.0 0.0 0.0 0.7670465030043039 8.78708368994248E-5 80-81 0.0 0.0 0.0 0.8822451701794498 8.78708368994248E-5 82-83 0.0 0.0 0.0 1.0145786505499834 8.78708368994248E-5 84-85 0.0 0.0 0.0 1.158862564738839 8.78708368994248E-5 86-87 0.0 0.0 0.0 1.375200565185223 8.78708368994248E-5 88-89 0.0 0.0 0.0 1.6381760123159765 8.78708368994248E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11010 0.0 30.851074 1 GGTATCA 7490 0.0 30.127518 1 ATCAACG 13575 0.0 24.877947 3 TCAACGC 13680 0.0 24.721176 4 TATCAAC 13790 0.0 24.491688 2 CAACGCA 13910 0.0 24.380709 5 AACGCAG 14205 0.0 23.840948 6 GTACATG 9070 0.0 21.631882 1 ACGCAGA 16080 0.0 20.79514 7 GAGTACT 8310 0.0 20.71964 12-13 CGCAGAG 16065 0.0 20.666723 8 TACATGG 9140 0.0 20.632778 2 AGTACTT 8895 0.0 20.131245 12-13 ACATGGG 8945 0.0 20.072258 3 AGAGTAC 13630 0.0 19.56722 10-11 GCAGAGT 16990 0.0 19.261986 9 GTACTTT 8835 0.0 19.085424 14-15 GTATAGA 740 0.0 17.975372 1 TACTTTT 10240 0.0 17.417667 14-15 AGTACAT 7045 0.0 16.384706 2 >>END_MODULE