FastQCFastQC Report
Thu 26 May 2016
SRR936858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262112
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.3408319305144927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.2424283147784018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36460.16117681175821533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96300.033.8025361
GGTATCA63700.032.675311
ATCAACG114400.028.1214713
TATCAAC115650.027.7770462
TCAACGC116400.027.679724
CAACGCA117500.027.3427315
AACGCAG119000.027.073766
ACGCAGA135450.023.5411977
GTACATG89250.023.534181
CGCAGAG135350.023.2090328
TACATGG87450.022.8230922
GAGTACT67100.021.84685112-13
ACATGGG87750.021.6073343
GCAGAGT143950.021.393279
GTACTTT70800.020.26709214-15
CATGGGG40550.019.8052754
GTACCGT1456.132165E-419.6629666
AGTACTT71050.019.0271812-13
AGAGTAC119500.018.98828310-11
CAGAGTA141650.017.62935310-11
Produced by FastQC (version 0.11.5)