##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936858_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262112 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.622138072739105 33.0 31.0 34.0 30.0 34.0 2 31.74201542629189 33.0 31.0 34.0 30.0 34.0 3 31.758730779024205 33.0 31.0 34.0 30.0 34.0 4 35.343837970887385 37.0 35.0 37.0 33.0 37.0 5 35.34280265521778 37.0 35.0 37.0 33.0 37.0 6 35.41290793736119 37.0 35.0 37.0 33.0 37.0 7 35.33981031885247 37.0 35.0 37.0 33.0 37.0 8 35.28877571048648 37.0 35.0 37.0 33.0 37.0 9 36.909512437934104 39.0 37.0 39.0 33.0 39.0 10-11 36.70431393317395 39.0 37.0 39.0 32.0 39.0 12-13 36.62606427091143 39.0 37.0 39.0 32.0 39.0 14-15 37.76769098965922 40.0 37.5 41.0 32.0 41.0 16-17 37.69697941569648 40.0 37.0 41.0 32.0 41.0 18-19 37.65039927289188 40.0 37.0 41.0 32.0 41.0 20-21 37.562600127668304 40.0 37.0 41.0 32.0 41.0 22-23 37.45411367783735 40.0 37.0 41.0 32.0 41.0 24-25 37.28658152204665 40.0 37.0 41.0 31.5 41.0 26-27 37.39037014966544 40.0 37.0 41.0 32.0 41.0 28-29 37.34998908100041 40.0 37.0 41.0 31.0 41.0 30-31 37.21587635802294 40.0 37.0 41.0 31.0 41.0 32-33 37.027873288325246 39.5 36.0 41.0 30.5 41.0 34-35 36.86920099446888 39.0 36.0 41.0 30.0 41.0 36-37 36.69378704502695 39.0 36.0 41.0 30.0 41.0 38-39 36.53418707827022 39.0 35.5 41.0 30.0 41.0 40-41 36.36505995282285 39.0 35.0 41.0 30.0 41.0 42-43 36.15393159136241 39.0 35.0 41.0 29.5 41.0 44-45 35.925090137004716 39.0 35.0 40.0 28.5 41.0 46-47 35.59940710274292 39.0 35.0 40.0 27.0 41.0 48-49 35.29065448571954 38.0 35.0 40.0 26.0 41.0 50-51 34.64958277927883 38.0 34.0 39.5 25.0 40.5 52-53 34.886313763421086 38.0 34.5 40.0 26.0 40.5 54-55 35.26898513424622 39.0 35.0 40.0 26.0 41.0 56-57 35.15616401840404 39.0 35.0 40.0 26.0 41.0 58-59 34.91006833437071 38.0 34.0 40.0 26.0 41.0 60-61 34.61595203950998 38.0 34.0 40.0 25.5 41.0 62-63 34.284531667751196 37.5 34.0 40.0 24.5 41.0 64-65 33.96696229010765 37.0 34.0 40.0 23.5 41.0 66-67 33.58447437615821 36.5 33.0 39.5 22.5 41.0 68-69 33.19675352060375 36.0 33.0 39.0 22.0 41.0 70-71 32.78460107191863 35.5 32.0 39.0 21.5 40.5 72-73 32.386754059922765 35.0 32.0 38.0 20.5 40.0 74-75 31.956857794839514 35.0 31.5 37.0 19.5 39.5 76-77 31.515235319913426 35.0 31.0 37.0 18.0 39.0 78-79 31.067419959754424 35.0 31.0 36.0 15.5 39.0 80-81 30.635316907385665 35.0 31.0 36.0 11.0 37.5 82-83 30.222151688333735 34.0 30.0 35.5 7.5 37.0 84-85 29.85641316610318 34.0 30.0 35.0 6.5 37.0 86-87 29.512805953020894 34.0 30.0 35.0 2.0 36.0 88-89 29.160139948861946 34.0 29.0 35.0 2.0 36.0 90-91 28.79767999992927 34.0 29.0 35.0 2.0 35.5 92-93 28.42310482416432 34.0 29.0 35.0 2.0 35.0 94-95 27.981431511790753 34.0 28.5 35.0 2.0 35.0 96-97 27.48900408114187 34.0 27.0 35.0 2.0 35.0 98-99 26.863117078199487 33.5 26.0 35.0 2.0 35.0 100-101 24.5828077920103 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45067709597298133 1101 2 0.4508640558324508 1101 3 0.43281683045463737 1101 4 0.399663068689307 1101 5 0.4116251649408298 1101 6 0.24896727429903365 1101 7 0.3119710636134059 1101 8 0.3184447867225444 1101 9 0.3297899855384756 1101 10-11 0.42793764187646843 1101 12-13 0.4273077534457954 1101 14-15 0.7596033621210196 1101 16-17 0.8787700179551976 1101 18-19 0.6811751434447828 1101 20-21 0.6548288416214589 1101 22-23 0.7413744678065157 1101 24-25 0.8533578967929571 1101 26-27 0.7720764585207291 1101 28-29 0.8430303415473404 1101 30-31 0.7324975333434836 1101 32-33 0.8188974323085816 1101 34-35 0.8020644708795572 1101 36-37 0.7041964197989401 1101 38-39 0.7237649473066625 1101 40-41 0.7115283808326822 1101 42-43 0.6801010010244752 1101 44-45 0.8278144820676232 1101 46-47 0.7351593587479215 1101 48-49 0.8516122066856227 1101 50-51 0.8525829169820511 1101 52-53 0.894319703385122 1101 54-55 0.9901760872527134 1101 56-57 1.0081965799643129 1101 58-59 0.873926010563892 1101 60-61 0.9595964294452486 1101 62-63 0.9271484713207059 1101 64-65 1.0337639120687996 1101 66-67 1.027117555898215 1101 68-69 0.9602114656961689 1101 70-71 0.9103563320939827 1101 72-73 0.885376527418714 1101 74-75 0.8643985867743211 1101 76-77 0.9386177922156733 1101 78-79 0.8544946617001905 1101 80-81 0.7904518617520147 1101 82-83 0.96969242235674 1101 84-85 1.0055562863397824 1101 86-87 0.9192769253265318 1101 88-89 0.7829293961628814 1101 90-91 0.9031441122355197 1101 92-93 0.9442344902094995 1101 94-95 0.9563146037991039 1101 96-97 1.0547398823068441 1101 98-99 1.1591330332069845 1101 100-101 1.2602693745982272 1102 1 0.5367671502584024 1102 2 0.4158222102364668 1102 3 0.430581042948031 1102 4 0.4298563805296425 1102 5 0.3206152199921206 1102 6 0.3136388616035575 1102 7 0.36365475973683914 1102 8 0.45209926349988194 1102 9 0.5678162684170616 1102 10-11 0.6435135845862092 1102 12-13 0.6450608290312445 1102 14-15 0.6752003756663143 1102 16-17 0.754299941063465 1102 18-19 0.8070014685771127 1102 20-21 0.8405541537659005 1102 22-23 0.7664279341177433 1102 24-25 0.8017633034049823 1102 26-27 0.7296725227576388 1102 28-29 0.7291763792495729 1102 30-31 0.7070382479698409 1102 32-33 0.7053691526507677 1102 34-35 0.6880557462834105 1102 36-37 0.5672344009140247 1102 38-39 0.7650936727022994 1102 40-41 0.7955465630210696 1102 42-43 0.718502879018736 1102 44-45 0.6379977292216097 1102 46-47 0.680589443828886 1102 48-49 0.7648672379547037 1102 50-51 0.7966423703619867 1102 52-53 0.7673196318414099 1102 54-55 0.7847067670804293 1102 56-57 0.8775204134890231 1102 58-59 0.8597958484660282 1102 60-61 0.7841396004677605 1102 62-63 0.8949675333659073 1102 64-65 0.9492265243181564 1102 66-67 0.7046415870180525 1102 68-69 0.6182958764347504 1102 70-71 0.9144031280287308 1102 72-73 0.8825342995962799 1102 74-75 0.7740941785799293 1102 76-77 0.8500789070907686 1102 78-79 0.8763752673970551 1102 80-81 1.0354678526108607 1102 82-83 0.8301562310484982 1102 84-85 0.8402828539548395 1102 86-87 0.7665468978354433 1102 88-89 0.7882442715937081 1102 90-91 0.8205750169869361 1102 92-93 1.0264412903733593 1102 94-95 1.15876582565539 1102 96-97 1.039801537850611 1102 98-99 1.140603680393589 1102 100-101 1.4010646790895471 1103 1 0.3993387896983016 1103 2 0.4219039856262192 1103 3 0.39288703668544045 1103 4 0.30532344604780093 1103 5 0.24313021101367127 1103 6 0.1594543300252269 1103 7 0.2536121342584252 1103 8 0.31756720883750233 1103 9 0.35216822160692374 1103 10-11 0.4509740613586999 1103 12-13 0.39395979381437485 1103 14-15 0.4629820369784099 1103 16-17 0.537831009618202 1103 18-19 0.37204833344038946 1103 20-21 0.5793571435082541 1103 22-23 0.7387417341514038 1103 24-25 0.7193078748535129 1103 26-27 0.7439939661995325 1103 28-29 0.6512795736666916 1103 30-31 0.5539104337383947 1103 32-33 0.685944382725431 1103 34-35 0.6179925094929786 1103 36-37 0.5427220889520825 1103 38-39 0.5611936441035041 1103 40-41 0.6604094690292612 1103 42-43 0.6166959988305294 1103 44-45 0.7602409849197471 1103 46-47 0.6230487839344008 1103 48-49 0.5872418688181398 1103 50-51 0.5697834435287845 1103 52-53 0.6328014936439956 1103 54-55 0.6068939459626748 1103 56-57 0.6655024158572544 1103 58-59 0.6167956902479617 1103 60-61 0.6276525944298825 1103 62-63 0.7538268390258338 1103 64-65 0.6105519770095569 1103 66-67 0.7008779771355975 1103 68-69 0.6872847434495739 1103 70-71 0.5845555422738826 1103 72-73 0.693845154009324 1103 74-75 0.5818185077923061 1103 76-77 0.5867090603157123 1103 78-79 0.6620857104057656 1103 80-81 0.6890916160302076 1103 82-83 0.746129456143489 1103 84-85 0.48361683921384824 1103 86-87 0.5175656484507094 1103 88-89 0.7634032882208039 1103 90-91 0.8232845246971259 1103 92-93 0.874028259406515 1103 94-95 0.9657485660632545 1103 96-97 1.019417372434095 1103 98-99 1.0790540511973319 1103 100-101 1.1424545435319722 1104 1 0.425990874005695 1104 2 0.402510393143114 1104 3 0.4759188066425928 1104 4 0.3801704141600055 1104 5 0.4157226587709246 1104 6 0.45158830425271645 1104 7 0.4801925983041997 1104 8 0.5085204873019151 1104 9 0.46358186696440384 1104 10-11 0.6541239548170665 1104 12-13 0.6494017653248463 1104 14-15 0.7834575427890229 1104 16-17 0.8021016862739003 1104 18-19 0.6910170376229487 1104 20-21 0.6947401611179345 1104 22-23 0.644120104233231 1104 24-25 0.7466737651147355 1104 26-27 0.5945341040036354 1104 28-29 0.6054357158231269 1104 30-31 0.7643261088692057 1104 32-33 0.6928527621162885 1104 34-35 0.6298629786775933 1104 36-37 0.5503070261685181 1104 38-39 0.5072576553614212 1104 40-41 0.45520863171530124 1104 42-43 0.5165279927957371 1104 44-45 0.5671741497943401 1104 46-47 0.5665992954920114 1104 48-49 0.6457772524429046 1104 50-51 0.6674353534789859 1104 52-53 0.5862014888107296 1104 54-55 0.6867044019217019 1104 56-57 0.5541786453400732 1104 58-59 0.4938604898080996 1104 60-61 0.7042296532178156 1104 62-63 0.7145964712360211 1104 64-65 0.7646204212940475 1104 66-67 0.7129987976809105 1104 68-69 0.6188765087572037 1104 70-71 0.6387685452459451 1104 72-73 0.6284252577568452 1104 74-75 0.6631842977931512 1104 76-77 0.5680617285898819 1104 78-79 0.6778277879885231 1104 80-81 0.7604597287963699 1104 82-83 0.7383066501830697 1104 84-85 0.6831012372411536 1104 86-87 0.5520199673661885 1104 88-89 0.7274073162114227 1104 90-91 0.744000435719439 1104 92-93 0.7654744314673039 1104 94-95 0.7729232945363833 1104 96-97 0.8451579461111898 1104 98-99 0.7488208339756355 1104 100-101 0.8947793559981285 1105 1 0.4044200688971209 1105 2 0.4981531145011111 1105 3 0.49698346700833085 1105 4 0.5110828348406145 1105 5 0.4433057265906726 1105 6 0.4878332371826488 1105 7 0.4933870613420268 1105 8 0.5960449725625665 1105 9 0.6085324680514432 1105 10-11 0.7059831210598091 1105 12-13 0.6885292386173063 1105 14-15 0.7726752460742503 1105 16-17 0.7030695301251342 1105 18-19 0.6742361867300204 1105 20-21 0.6623153036920328 1105 22-23 0.6559291486520848 1105 24-25 0.8275369743037331 1105 26-27 0.7682811922790833 1105 28-29 0.670265403364958 1105 30-31 0.36104741332180623 1105 32-33 0.5727757587159275 1105 34-35 0.7483582529821291 1105 36-37 0.598781041537066 1105 38-39 0.5013279651421456 1105 40-41 0.6085687752265088 1105 42-43 0.7594641892133112 1105 44-45 0.6943250775300243 1105 46-47 0.8446211970154778 1105 48-49 0.8172031005245088 1105 50-51 0.783260355487073 1105 52-53 0.7178542773726733 1105 54-55 0.8923051944462728 1105 56-57 1.0408593061342017 1105 58-59 0.8218421760647558 1105 60-61 0.8122203356992728 1105 62-63 0.8297859542208386 1105 64-65 0.985468529677135 1105 66-67 0.8615605262412416 1105 68-69 0.9894591749597836 1105 70-71 0.9787266699614676 1105 72-73 1.0092767932731945 1105 74-75 0.8555868788528898 1105 76-77 1.0001948512973158 1105 78-79 1.0198713232906762 1105 80-81 1.0709254440765612 1105 82-83 1.0764666009180281 1105 84-85 0.9493680716945541 1105 86-87 1.0203960952410966 1105 88-89 0.8747100016916214 1105 90-91 0.8308149689064059 1105 92-93 0.8853976422627738 1105 94-95 0.9433605217713854 1105 96-97 1.1181744124155095 1105 98-99 1.2088534988394883 1105 100-101 1.3329743277510566 1106 1 0.28734787511233506 1106 2 0.36431517989788276 1106 3 0.32432099602850073 1106 4 0.21632878600281913 1106 5 0.15971086211136054 1106 6 0.2852909866162321 1106 7 0.2996805915929315 1106 8 0.3658738444822305 1106 9 0.31074387472538234 1106 10-11 0.4529884635677135 1106 12-13 0.5033056644515526 1106 14-15 0.5950576130010532 1106 16-17 0.5931298920730512 1106 18-19 0.48285558199168577 1106 20-21 0.5498950636987416 1106 22-23 0.36208159194038814 1106 24-25 0.7022162839733994 1106 26-27 0.5177785489963824 1106 28-29 0.560134889533181 1106 30-31 0.6283502579974751 1106 32-33 0.6624835710848131 1106 34-35 0.5392082991346854 1106 36-37 0.3596844008836584 1106 38-39 0.3733520204133285 1106 40-41 0.43975246239610044 1106 42-43 0.40102174005104985 1106 44-45 0.491266701540539 1106 46-47 0.520388028712496 1106 48-49 0.5760905162611323 1106 50-51 0.46161195404375377 1106 52-53 0.48317832425248497 1106 54-55 0.5867860201574402 1106 56-57 0.5612681338593077 1106 58-59 0.515531527411575 1106 60-61 0.533647540532705 1106 62-63 0.557665230325 1106 64-65 0.4790786664565232 1106 66-67 0.5676060074865248 1106 68-69 0.5422854455536097 1106 70-71 0.4597956190817669 1106 72-73 0.6137444724989436 1106 74-75 0.44341502352017415 1106 76-77 0.6051356921129525 1106 78-79 0.6326621263451351 1106 80-81 0.6962361766434846 1106 82-83 0.5846072403407021 1106 84-85 0.5837643123591505 1106 86-87 0.6195368185775081 1106 88-89 0.6878455223763602 1106 90-91 0.6652122165358385 1106 92-93 0.6859825769499182 1106 94-95 0.7569925880072894 1106 96-97 0.7518655539220411 1106 98-99 0.7252798925067978 1106 100-101 0.7837042094326883 1107 1 0.2486850063681736 1107 2 0.2885375517405784 1107 3 0.3325073206442788 1107 4 0.3014635666608996 1107 5 0.3116707103794667 1107 6 0.21641498799321113 1107 7 0.2526274504110404 1107 8 0.35341783036709984 1107 9 0.4537571090889614 1107 10-11 0.5734643623752262 1107 12-13 0.4997603641383819 1107 14-15 0.5305390719195557 1107 16-17 0.5634826843632084 1107 18-19 0.5560648110931439 1107 20-21 0.7440086802552557 1107 22-23 0.6336058508290208 1107 24-25 0.7860548066335156 1107 26-27 0.6279477045532857 1107 28-29 0.7078365010953931 1107 30-31 0.598748200723378 1107 32-33 0.6762477364834183 1107 34-35 0.5568899806338266 1107 36-37 0.696698595960406 1107 38-39 0.4982654361935559 1107 40-41 0.6530486270097455 1107 42-43 0.6462247264990921 1107 44-45 0.6999480511289136 1107 46-47 0.5257513498640165 1107 48-49 0.5728566438315994 1107 50-51 0.5789676273722009 1107 52-53 0.751619909609829 1107 54-55 0.7524515585037008 1107 56-57 0.9339227614375858 1107 58-59 0.9526860852819894 1107 60-61 0.955016074467224 1107 62-63 0.9201092520439076 1107 64-65 0.8307228140680607 1107 66-67 0.7760061028971066 1107 68-69 0.7901205922826122 1107 70-71 0.7745413990026861 1107 72-73 0.7760743847507285 1107 74-75 0.7949551048834564 1107 76-77 0.762427366350579 1107 78-79 0.9355989749812714 1107 80-81 0.877528501047383 1107 82-83 0.7780525203251649 1107 84-85 0.8552041068960357 1107 86-87 0.8367092031614298 1107 88-89 0.9008202379120966 1107 90-91 0.9467130698790776 1107 92-93 1.0835023203544836 1107 94-95 0.9495856259862983 1107 96-97 0.8530646393724339 1107 98-99 1.008686754976587 1107 100-101 0.7998552911600392 1108 1 0.24721326613865457 1108 2 0.2846882684973231 1108 3 0.19839204463835713 1108 4 0.15708266999185838 1108 5 0.1977195519059478 1108 6 0.11623843541327261 1108 7 0.3203123573829103 1108 8 0.2240182670648565 1108 9 0.29767497979691626 1108 10-11 0.23616791650997726 1108 12-13 0.3501657471207551 1108 14-15 0.4148661958778064 1108 16-17 0.4792202018837983 1108 18-19 0.5112404453227555 1108 20-21 0.4773277497727477 1108 22-23 0.46363121200895563 1108 24-25 0.560537496324784 1108 26-27 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0.46827985782999093 1108 94-95 0.6435836170814362 1108 96-97 0.6374211635213705 1108 98-99 0.5729417478710026 1108 100-101 0.5544477458899131 1109 1 0.18549246708716893 1109 2 0.06461185184220142 1109 3 0.14130667321389367 1109 4 0.2815456661269735 1109 5 0.19275627604122292 1109 6 0.1335382576523756 1109 7 0.03140149788588076 1109 8 0.10726173619109147 1109 9 0.1517957733735713 1109 10-11 0.3271602380604506 1109 12-13 0.323123507093932 1109 14-15 0.3013187496295231 1109 16-17 0.3677065695562973 1109 18-19 0.32433465688730934 1109 20-21 0.34708399578425286 1109 22-23 0.3783884246701845 1109 24-25 0.40587353969912243 1109 26-27 0.16972592401793207 1109 28-29 0.31637270782520943 1109 30-31 0.40558339694602097 1109 32-33 0.3838464331395457 1109 34-35 0.49928854817713386 1109 36-37 0.4503127157452056 1109 38-39 0.393651710024713 1109 40-41 0.4554348601220042 1109 42-43 0.2674762555783161 1109 44-45 0.4123648318933135 1109 46-47 0.3224013090602966 1109 48-49 0.4674388255837627 1109 50-51 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-2.0314809664797764 2316 50-51 -2.0801537465775937 2316 52-53 -2.0730689793472266 2316 54-55 -2.1993473182144285 2316 56-57 -2.3780686253032712 2316 58-59 -2.2888333206239864 2316 60-61 -2.3793075178262413 2316 62-63 -2.103364026301165 2316 64-65 -2.6749319377946215 2316 66-67 -2.441811503365301 2316 68-69 -2.4220468632170835 2316 70-71 -2.3768900031978646 2316 72-73 -2.9359090267501813 2316 74-75 -3.059197694579133 2316 76-77 -3.2317568432878048 2316 78-79 -3.266950647881899 2316 80-81 -3.394626562156393 2316 82-83 -3.0945844314154343 2316 84-85 -3.284328850783048 2316 86-87 -3.772178895019909 2316 88-89 -3.8431178128779138 2316 90-91 -3.372764306512373 2316 92-93 -3.5048618045623137 2316 94-95 -4.052658177098895 2316 96-97 -4.172762868457102 2316 98-99 -4.227874198862111 2316 100-101 -4.416753759510339 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5513.0 3 2124.0 4 2201.0 5 2370.0 6 2615.0 7 2910.0 8 3563.0 9 4519.0 10 5472.0 11 6728.0 12 8186.0 13 9534.0 14 10689.0 15 12528.0 16 15237.0 17 19091.0 18 17223.0 19 13073.0 20 12926.0 21 13664.0 22 15115.0 23 17057.0 24 19277.0 25 22218.0 26 25849.0 27 30278.0 28 35865.0 29 43476.0 30 53108.0 31 65454.0 32 82625.0 33 107516.0 34 144623.0 35 204314.0 36 306518.0 37 448367.0 38 399220.0 39 71033.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64957428252497 18.933990351701187 14.292935725771786 23.123499640002052 2 15.260030019963185 20.27072805928987 40.43434305016624 24.0348988705807 3 19.195083265494908 24.757406695818396 29.524108103423348 26.52340193526335 4 12.761104818733074 16.641583149537418 37.39777711242207 33.19953491930744 5 13.850664505615255 37.28760326776454 34.10074545657225 14.760986770047968 6 31.60697949715204 36.29465920894078 17.692664308553084 14.405696985354096 7 28.173869423201463 32.68759488564179 21.07058430091621 18.067951390240538 8 25.929042172847893 35.081975480346856 19.940908290622563 19.04807405618269 9 26.089551103176024 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28.923701590091788 22.145894436579805 34-35 22.368042054117264 26.923145791217134 28.673705897501932 22.035106257163665 36-37 22.440455269888215 26.778914699894564 28.672674743750427 22.107955286466797 38-39 22.174437425200395 26.93902011471004 28.774862647261546 22.11167981282802 40-41 22.39701366372195 26.907686539911985 28.685393973744223 22.009905822621846 42-43 22.200559775311795 26.975935674027035 28.483000947400484 22.340503603260682 44-45 22.264152694999908 27.063744044192994 28.41464794759509 22.25745531321201 46-47 22.401942320988702 27.193532728922087 28.062506520674297 22.342018429414917 48-49 22.524812329885624 27.30956583782809 27.864364715498695 22.301257116787596 50-51 22.406400256059758 27.36530337245554 27.88995619751261 22.33834017397209 52-53 22.51200160566652 27.499315867583796 27.639701165655534 22.348981361094154 54-55 22.390673724711917 27.35800112338662 27.684649768414797 22.566675383486658 56-57 22.38788778143849 27.623056035437628 27.603622469712157 22.385433713411725 58-59 22.598982832563948 27.414181056864685 27.591158552692807 22.39567755787856 60-61 22.50874654421689 27.232355746963165 27.98215428627647 22.27674342254348 62-63 22.365178645051657 27.371895754702695 27.954141053333874 22.308784546911774 64-65 22.354732273400295 27.457632963590278 27.809514461891883 22.37812030111754 66-67 22.32099313477064 27.512295025792692 27.591992172214812 22.574719667221856 68-69 22.177580096588834 27.468851173023936 27.84982498994557 22.503743740441664 70-71 22.305807685097083 27.332571638786995 27.77415697746398 22.58746369865194 72-73 22.353023625166692 27.333909312468386 27.67525211614852 22.637814946216402 74-75 22.23264252060975 27.71386685998015 27.49855578643812 22.554934832971977 76-77 22.362696985749903 27.544221471280917 27.47455402665007 22.618527516319116 78-79 22.394965211365335 27.50676955475943 27.485674812833917 22.61259042104132 80-81 22.2867072518462 27.66056945577932 27.44527622958921 22.607447062785273 82-83 22.35496782221892 27.619206816480297 27.48864281093138 22.537182550369405 84-85 22.412899229138805 27.528789290054682 27.362886218216925 22.695425262589588 86-87 22.347626852244293 27.507147422738083 27.498369983013333 22.646855742004295 88-89 22.475399503137282 27.412876026849247 27.36039083998628 22.751333630027187 90-91 22.401313207797447 27.46956574605789 27.451415180372628 22.677705865772037 92-93 22.301433227348507 27.44935502558229 27.50770564225478 22.741506104814423 94-95 22.289793134401286 27.416805494601466 27.440197126455207 22.853204244542045 96-97 22.184589761710384 27.428955801305328 27.509665230554774 22.876789206429514 98-99 22.15561472726951 27.462049503724277 27.342094088922703 23.040241680083508 100-101 22.299799457182992 27.410157724813324 27.351014513973965 22.939028304029723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 334.0 1 259.5 2 241.5 3 308.0 4 286.5 5 310.5 6 520.0 7 1057.5 8 1793.0 9 2206.5 10 2082.5 11 1962.5 12 2047.5 13 2158.5 14 2310.5 15 2483.0 16 2647.0 17 2725.5 18 2900.0 19 3220.5 20 3653.5 21 4280.0 22 5211.0 23 6420.5 24 8107.5 25 10405.0 26 13414.5 27 16940.0 28 19796.5 29 22718.5 30 26836.5 31 30924.5 32 34828.5 33 39267.0 34 44412.5 35 48940.5 36 53691.5 37 58290.0 38 61506.0 39 64271.5 40 67263.0 41 70954.5 42 73988.0 43 78050.5 44 82461.5 45 85155.0 46 86701.0 47 88205.0 48 92008.5 49 90629.0 50 84246.0 51 79420.5 52 76621.0 53 75252.0 54 72083.5 55 67152.0 56 61680.5 57 56116.5 58 51036.5 59 45957.5 60 39428.5 61 31795.0 62 24718.5 63 19401.0 64 15069.0 65 11419.5 66 8510.5 67 6524.0 68 4731.5 69 3068.0 70 2110.0 71 1447.5 72 1003.0 73 750.0 74 532.5 75 347.5 76 235.0 77 136.0 78 76.5 79 45.5 80 31.5 81 26.5 82 22.0 83 19.5 84 19.5 85 12.5 86 7.0 87 8.0 88 7.5 89 4.0 90 2.0 91 4.0 92 5.5 93 3.0 94 2.5 95 2.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043675998359055605 2 0.0419961522683227 3 0.027010156879942283 4 0.044162269595846716 5 0.06560241049072725 6 0.06843162495933004 7 0.06613288820359027 8 0.06887368972004923 9 0.06843162495933004 10-11 0.07607934531977197 12-13 0.07402374418242776 14-15 0.068453728197366 16-17 0.056650599086163725 18-19 0.05331301014273387 20-21 0.05928088441244289 22-23 0.03264648257911191 24-25 0.01878775233056542 26-27 0.01556067957731536 28-29 0.01878775233056542 30-31 0.014831272722128702 32-33 0.010167489496541286 34-35 0.011692612921022479 36-37 0.006940416743291225 38-39 0.010388521876900878 40-41 0.009283359975102913 42-43 0.005879461317565178 44-45 0.0015030201864452335 46-47 0.0033596921814658164 48-49 0.0013704007582294776 50-51 0.0061889066500686075 52-53 0.005437396556845992 54-55 0.008907604928491604 56-57 0.024799833076346353 58-59 0.010720070447440269 60-61 0.022302167178282952 62-63 0.01549436986320748 64-65 0.01197995501548995 66-67 0.01878775233056542 68-69 0.029640442206221446 70-71 0.021572760323096293 72-73 0.020158153088794897 74-75 0.022125341273995278 76-77 0.028911035351034786 78-79 0.03912273132364799 80-81 0.03437053514591674 82-83 0.03399478009930543 84-85 0.024335665077591207 86-87 0.027960596115488533 88-89 0.023053677271505566 90-91 0.02097597289612539 92-93 0.03456946428824037 94-95 0.027385911926553593 96-97 0.04040471912973363 98-99 0.0562306375634805 100-101 0.06619919791769815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2262112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.62650964279903 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8868077297742 46.441184921462145 2 12.761650659958615 15.2186537226811 3 4.07352356266857 7.286699759688798 4 1.7797599066713718 4.2448348494803065 5 0.9211950546169625 2.746382290350855 6 0.5641190187107733 2.0181868865306547 7 0.36754172208985675 1.5340661025425266 8 0.2804731722150498 1.3378909046101677 9 0.2027530724911705 1.088051222880172 >10 1.0686871601935652 11.56040776610038 >50 0.06665220567783692 2.711144586398836 >100 0.025574661271625367 2.5734776970573696 >500 8.166358978874046E-4 0.31592999908103986 >1k 2.9695850832261664E-4 0.33903711124578606 >5k 1.4847925416130832E-4 0.5840521798899205 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7710 0.3408319305144927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.2424283147784018 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3646 0.16117681175821533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.420647607191863E-5 4.420647607191863E-5 20-21 0.0 0.0 0.0 1.7682590428767453E-4 4.420647607191863E-5 22-23 0.0 0.0 0.0 3.5365180857534906E-4 4.420647607191863E-5 24-25 0.0 0.0 0.0 7.294068551866574E-4 4.420647607191863E-5 26-27 0.0 0.0 0.0 0.0010609554257260472 6.630971410787795E-5 28-29 0.0 0.0 0.0 0.00194508494716442 8.841295214383726E-5 30-31 0.0 0.0 0.0 0.004111202274688433 8.841295214383726E-5 32-33 0.0 0.0 0.0 0.006741487600967591 8.841295214383726E-5 34-35 0.0 0.0 0.0 0.009128637308851197 8.841295214383726E-5 36-37 0.0 0.0 0.0 0.01129475463637521 8.841295214383726E-5 38-39 0.0 0.0 0.0 0.014367104723373556 8.841295214383726E-5 40-41 0.0 0.0 0.0 0.018522513474133907 8.841295214383726E-5 42-43 8.841295214383726E-5 0.0 0.0 0.02449038774384292 8.841295214383726E-5 44-45 8.841295214383726E-5 0.0 0.0 0.029839371348545078 8.841295214383726E-5 46-47 1.1051619017979659E-4 0.0 0.0 0.03593986504646984 8.841295214383726E-5 48-49 1.326194282157559E-4 0.0 0.0 0.04159829398367543 8.841295214383726E-5 50-51 1.326194282157559E-4 0.0 0.0 0.048958672249649886 1.326194282157559E-4 52-53 1.326194282157559E-4 0.0 0.0 0.06869686381576155 1.326194282157559E-4 54-55 1.326194282157559E-4 0.0 0.0 0.09369562603443155 1.326194282157559E-4 56-57 1.326194282157559E-4 0.0 0.0 0.115179973405384 1.326194282157559E-4 58-59 1.326194282157559E-4 4.420647607191863E-5 0.0 0.15357329787384533 1.326194282157559E-4 60-61 1.326194282157559E-4 4.420647607191863E-5 0.0 0.20100684669901403 1.326194282157559E-4 62-63 1.326194282157559E-4 4.420647607191863E-5 0.0 0.23228292851989646 1.326194282157559E-4 64-65 1.326194282157559E-4 4.420647607191863E-5 0.0 0.25904994978144313 1.326194282157559E-4 66-67 1.326194282157559E-4 8.841295214383726E-5 0.0 0.3305318215897356 1.5472266625171522E-4 68-69 1.326194282157559E-4 8.841295214383726E-5 0.0 0.40917514252167886 1.7682590428767453E-4 70-71 1.326194282157559E-4 8.841295214383726E-5 0.0 0.4406501534848849 1.7682590428767453E-4 72-73 1.326194282157559E-4 8.841295214383726E-5 0.0 0.5002404832298313 1.7682590428767453E-4 74-75 1.326194282157559E-4 8.841295214383726E-5 0.0 0.5924993987919254 1.7682590428767453E-4 76-77 1.326194282157559E-4 8.841295214383726E-5 0.0 0.6862834377785008 1.7682590428767453E-4 78-79 1.326194282157559E-4 8.841295214383726E-5 0.0 0.769877884030499 1.7682590428767453E-4 80-81 1.326194282157559E-4 8.841295214383726E-5 0.0 0.881211894017626 1.7682590428767453E-4 82-83 1.326194282157559E-4 8.841295214383726E-5 0.0 1.008349719200464 1.7682590428767453E-4 84-85 1.326194282157559E-4 8.841295214383726E-5 0.0 1.1501198879631072 1.7682590428767453E-4 86-87 1.326194282157559E-4 8.841295214383726E-5 0.0 1.3599459266384688 1.7682590428767453E-4 88-89 1.326194282157559E-4 8.841295214383726E-5 0.0 1.6172497206150713 1.7682590428767453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9630 0.0 33.802536 1 GGTATCA 6370 0.0 32.67531 1 ATCAACG 11440 0.0 28.121471 3 TATCAAC 11565 0.0 27.777046 2 TCAACGC 11640 0.0 27.67972 4 CAACGCA 11750 0.0 27.342731 5 AACGCAG 11900 0.0 27.07376 6 ACGCAGA 13545 0.0 23.541197 7 GTACATG 8925 0.0 23.53418 1 CGCAGAG 13535 0.0 23.209032 8 TACATGG 8745 0.0 22.823092 2 GAGTACT 6710 0.0 21.846851 12-13 ACATGGG 8775 0.0 21.607334 3 GCAGAGT 14395 0.0 21.39327 9 GTACTTT 7080 0.0 20.267092 14-15 CATGGGG 4055 0.0 19.805275 4 GTACCGT 145 6.132165E-4 19.662966 6 AGTACTT 7105 0.0 19.02718 12-13 AGAGTAC 11950 0.0 18.988283 10-11 CAGAGTA 14165 0.0 17.629353 10-11 >>END_MODULE