FastQCFastQC Report
Thu 26 May 2016
SRR936858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262112
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89480.3955595478915279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.3097989843120058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.2094944901048224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.032.0085071
GTATCAA106450.031.6967721
ATCAACG134050.024.982313
TATCAAC134450.024.9096372
GTACATG84300.024.522461
TCAACGC136200.024.480074
CAACGCA137850.024.1870565
AACGCAG141350.023.6543126
ACATGGG83250.023.3372333
TACATGG85800.023.1988162
ACGCAGA159200.020.9130827
CGCAGAG157550.020.80058
GAGTACT86450.020.27326212-13
AGTACTT93000.019.58595312-13
AGAGTAC135600.019.5792110-11
GCAGAGT166850.019.128739
GTACTTT91950.018.8806614-15
TAGACCG1559.634545E-418.3853655
CATGGGG41450.018.219034
CATGGGA49650.018.0798684
Produced by FastQC (version 0.11.5)