##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936858_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262112 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.160815202783944 33.0 31.0 34.0 31.0 34.0 2 32.22828224243539 33.0 31.0 34.0 30.0 34.0 3 32.378911389002845 34.0 31.0 34.0 31.0 34.0 4 36.05861292455899 37.0 35.0 37.0 35.0 37.0 5 35.96844895389795 37.0 35.0 37.0 35.0 37.0 6 36.00041244642175 37.0 35.0 37.0 35.0 37.0 7 35.94878723953544 37.0 35.0 37.0 35.0 37.0 8 35.93372255661966 37.0 35.0 37.0 35.0 37.0 9 37.629405175340565 39.0 37.0 39.0 35.0 39.0 10-11 37.52136985259793 39.0 37.0 39.0 35.0 39.0 12-13 37.445284318371506 39.0 37.0 39.0 34.5 39.0 14-15 38.67001655974594 40.0 38.0 41.0 34.0 41.0 16-17 38.586559816666906 40.0 38.0 41.0 34.0 41.0 18-19 38.617003048478594 40.0 38.0 41.0 34.0 41.0 20-21 38.71483463241431 40.0 38.0 41.0 34.0 41.0 22-23 38.65054802768387 40.0 38.0 41.0 34.0 41.0 24-25 38.56750704651229 40.0 38.0 41.0 34.0 41.0 26-27 38.40446715282002 40.0 38.0 41.0 34.0 41.0 28-29 38.252473573368604 40.0 38.0 41.0 34.0 41.0 30-31 38.221074818576625 40.0 38.0 41.0 34.0 41.0 32-33 38.14776191452943 40.0 38.0 41.0 34.0 41.0 34-35 38.036629044008436 40.0 37.0 41.0 33.0 41.0 36-37 37.87589341288141 40.0 37.0 41.0 33.0 41.0 38-39 37.7403002150203 40.0 37.0 41.0 33.0 41.0 40-41 37.51980184889165 40.0 37.0 41.0 33.0 41.0 42-43 37.303706668812154 40.0 37.0 41.0 32.0 41.0 44-45 37.08120331796127 40.0 36.0 41.0 31.0 41.0 46-47 36.760549433449796 39.5 36.0 41.0 31.0 41.0 48-49 36.41852724356707 39.0 36.0 41.0 30.0 41.0 50-51 36.181960265451046 39.0 35.0 40.5 30.0 41.0 52-53 35.96012023277362 39.0 35.0 40.0 29.0 41.0 54-55 35.698310914755766 39.0 35.0 40.0 28.0 41.0 56-57 35.4488811782971 38.0 35.0 40.0 28.0 41.0 58-59 35.107582648427666 38.0 34.0 40.0 27.5 41.0 60-61 34.77430715190053 38.0 34.0 40.0 26.5 41.0 62-63 34.433869322120216 37.0 34.0 40.0 26.0 41.0 64-65 34.02332952568219 37.0 33.0 39.5 26.0 41.0 66-67 33.58853916163302 36.0 33.0 39.0 25.0 40.0 68-69 33.148433410900964 35.5 32.0 39.0 24.5 40.0 70-71 32.74635827050119 35.0 32.0 38.0 24.0 40.0 72-73 32.34661811616755 35.0 31.5 37.5 22.5 39.5 74-75 31.858561821872655 35.0 31.0 37.0 21.5 39.0 76-77 30.40615827156215 33.5 29.5 35.5 20.0 38.0 78-79 31.11439796084367 34.0 31.0 36.0 20.0 38.0 80-81 30.843326501959233 34.0 31.0 36.0 18.0 37.0 82-83 30.620572500389017 34.0 31.0 35.0 18.0 37.0 84-85 30.282313828846668 34.0 30.0 35.0 17.5 36.5 86-87 29.90123477528964 34.0 30.0 35.0 13.0 36.0 88-89 29.51478419282511 34.0 29.5 35.0 7.0 36.0 90-91 29.05610619633334 34.0 29.0 35.0 3.5 35.0 92-93 28.60228825982091 34.0 29.0 35.0 2.0 35.0 94-95 28.111595491293095 33.0 28.5 35.0 2.0 35.0 96-97 27.458746074464926 33.0 27.0 35.0 2.0 35.0 98-99 26.641992748369667 33.0 25.5 34.5 2.0 35.0 100-101 23.31135505226974 29.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32817112615958166 1101 2 0.3133119090777896 1101 3 0.2820425932012469 1101 4 0.22439769906223006 1101 5 0.2947575024111728 1101 6 0.24119992908445909 1101 7 0.27907093246281534 1101 8 0.32229713405227756 1101 9 0.42232215082589875 1101 10-11 0.33733887335159096 1101 12-13 0.3929721164021629 1101 14-15 0.3920454615718114 1101 16-17 0.37954485735371435 1101 18-19 0.2769710418937805 1101 20-21 0.3090350044240253 1101 22-23 0.45881169744130545 1101 24-25 0.3920037505245162 1101 26-27 0.31861337087546104 1101 28-29 0.33356320219058233 1101 30-31 0.3772779442397436 1101 32-33 0.5202406623063567 1101 34-35 0.5583355949724904 1101 36-37 0.5587841409828016 1101 38-39 0.5116882030261749 1101 40-41 0.6123315482353746 1101 42-43 0.6252891539033527 1101 44-45 0.5390513400325858 1101 46-47 0.5646358100698237 1101 48-49 0.3684820776260196 1101 50-51 0.359085775582038 1101 52-53 0.35406851477136314 1101 54-55 0.4059970073630481 1101 56-57 0.33317873000240894 1101 58-59 0.35658250878299214 1101 60-61 0.29265684712795803 1101 62-63 0.24263717333846557 1101 64-65 0.3266866289926895 1101 66-67 0.18513036805659766 1101 68-69 0.23483233323351982 1101 70-71 0.1701248021944579 1101 72-73 0.19630797752459728 1101 74-75 0.11104250106309266 1101 76-77 0.06780922339842377 1101 78-79 0.3502529401790646 1101 80-81 0.54358138771277 1101 82-83 0.5382108989308314 1101 84-85 0.3957707639416803 1101 86-87 0.3397780136009594 1101 88-89 0.24808908078280112 1101 90-91 0.12605053345023265 1101 92-93 0.32891860369987924 1101 94-95 0.36296869832233014 1101 96-97 0.624464998584731 1101 98-99 0.7128871892785682 1101 100-101 1.0389088511990074 1102 1 0.3266186464229861 1102 2 0.26461287439478554 1102 3 0.22625045774118036 1102 4 0.172279510354592 1102 5 0.2313862172900798 1102 6 0.2772478697185008 1102 7 0.2997859174694568 1102 8 0.24211961599870335 1102 9 0.2934125348101233 1102 10-11 0.28234614073507913 1102 12-13 0.2863787194565717 1102 14-15 0.35227481985838693 1102 16-17 0.32546547447515906 1102 18-19 0.34204471229697475 1102 20-21 0.25570178690556133 1102 22-23 0.3669261359885283 1102 24-25 0.29868245820234307 1102 26-27 0.37153871455143417 1102 28-29 0.4026908602587369 1102 30-31 0.4896525582913398 1102 32-33 0.5180789402811072 1102 34-35 0.4381030349649748 1102 36-37 0.4702066820321704 1102 38-39 0.4349033136546012 1102 40-41 0.42557884605948715 1102 42-43 0.3857273899232396 1102 44-45 0.46567486378985734 1102 46-47 0.3628882569859826 1102 48-49 0.5268230556751732 1102 50-51 0.38691627714825216 1102 52-53 0.35313181525997805 1102 54-55 0.30419143476216703 1102 56-57 0.2528813809115249 1102 58-59 0.2826115473084343 1102 60-61 0.3896054800547688 1102 62-63 0.2480198565113625 1102 64-65 0.28498103825369725 1102 66-67 0.262601086973703 1102 68-69 0.3089592711946736 1102 70-71 0.21625059844508598 1102 72-73 0.19472794505533386 1102 74-75 0.408014353004603 1102 76-77 0.5773797351376615 1102 78-79 0.4369392542790962 1102 80-81 0.4476722866995928 1102 82-83 0.4129810204112978 1102 84-85 0.26914970592047993 1102 86-87 0.3948253846873264 1102 88-89 0.24256172241365093 1102 90-91 0.295470792199346 1102 92-93 0.4258720933795601 1102 94-95 0.572289423525195 1102 96-97 0.852746113418732 1102 98-99 0.9807745634209546 1102 100-101 1.1223273178063131 1103 1 0.25577095064400623 1103 2 0.2536366128952565 1103 3 0.2131527292258184 1103 4 0.16252845816709538 1103 5 0.24078646248138114 1103 6 0.2846400343938029 1103 7 0.17069006366070738 1103 8 0.15029186858497212 1103 9 0.1278947703958906 1103 10-11 0.16599179129806174 1103 12-13 0.21592077809588517 1103 14-15 0.2952924997464379 1103 16-17 0.38963700303165183 1103 18-19 0.38359675492581147 1103 20-21 0.33536234097514495 1103 22-23 0.3697375421100162 1103 24-25 0.30073565048063955 1103 26-27 0.319490948760496 1103 28-29 0.2714745668241889 1103 30-31 0.3575324418264074 1103 32-33 0.35393965309178554 1103 34-35 0.347278113621023 1103 36-37 0.371201868056076 1103 38-39 0.3137943899502602 1103 40-41 0.4085140844354598 1103 42-43 0.33042298453082 1103 44-45 0.39139886087505715 1103 46-47 0.5040829360022485 1103 48-49 0.6291227094820968 1103 50-51 0.6561458896671652 1103 52-53 0.5504265665484596 1103 54-55 0.4588710749365461 1103 56-57 0.5065003622972739 1103 58-59 0.5948449045706212 1103 60-61 0.4887955044337957 1103 62-63 0.47234318474697545 1103 64-65 0.4063268220598246 1103 66-67 0.49667051724483 1103 68-69 0.4171491388500215 1103 70-71 0.4465618359812069 1103 72-73 0.519695428160837 1103 74-75 0.42916448438911203 1103 76-77 0.5072563493308486 1103 78-79 0.5290594342554122 1103 80-81 0.7476182459839364 1103 82-83 0.5147356905060825 1103 84-85 0.39642894735545653 1103 86-87 0.44048007590898663 1103 88-89 0.4091246226871412 1103 90-91 0.4226718585928424 1103 92-93 0.4065842465256786 1103 94-95 0.6205378075807779 1103 96-97 0.8622886054298426 1103 98-99 0.8164413797129697 1103 100-101 0.9009097287768917 1104 1 0.28428683941729815 1104 2 0.2350123490450926 1104 3 0.2133682291206611 1104 4 0.1374278133251039 1104 5 0.10772616225547438 1104 6 0.17533425362410782 1104 7 0.09433799155632272 1104 8 0.16912484178649123 1104 9 0.26732060597726104 1104 10-11 0.15735701608601715 1104 12-13 0.24029513436735073 1104 14-15 0.2586137785336007 1104 16-17 0.2889060809137902 1104 18-19 0.25287090374616383 1104 20-21 0.32371695833501235 1104 22-23 0.300308628814669 1104 24-25 0.25400622587734034 1104 26-27 0.42582207203822264 1104 28-29 0.2253492010086191 1104 30-31 0.2780102843828587 1104 32-33 0.2634059303540752 1104 34-35 0.23886069571720014 1104 36-37 0.23875431451913443 1104 38-39 0.29207803215012973 1104 40-41 0.37879836654419563 1104 42-43 0.40020890066401194 1104 44-45 0.3856059281132218 1104 46-47 0.30651896359022857 1104 48-49 0.4788577684389921 1104 50-51 0.4797395531314166 1104 52-53 0.42845192842950297 1104 54-55 0.3453986965706619 1104 56-57 0.290423770558931 1104 58-59 0.36540062866923506 1104 60-61 0.40516057443731057 1104 62-63 0.43421138708839635 1104 64-65 0.3319161463667939 1104 66-67 0.33565215142107974 1104 68-69 0.4401045711150502 1104 70-71 0.3997453326437608 1104 72-73 0.33517489146025525 1104 74-75 0.32742117212576716 1104 76-77 0.2674811602591518 1104 78-79 0.44034108343076994 1104 80-81 0.4538994787392028 1104 82-83 0.5343779367140122 1104 84-85 0.3593476867190226 1104 86-87 0.5341592183352439 1104 88-89 0.4797990015471427 1104 90-91 0.47001053475242216 1104 92-93 0.4469302606663881 1104 94-95 0.5023669722860085 1104 96-97 0.7069277609418769 1104 98-99 0.9903374120982669 1104 100-101 0.9515956435719524 1105 1 0.3075701401750166 1105 2 0.2697669074000686 1105 3 0.23428852149797308 1105 4 0.20695599346380078 1105 5 0.26581167994002186 1105 6 0.215840612562765 1105 7 0.2112822738664235 1105 8 0.23257542948629606 1105 9 0.23755938077712102 1105 10-11 0.31309955270011613 1105 12-13 0.3149266372188251 1105 14-15 0.42589333086431935 1105 16-17 0.4176020031607024 1105 18-19 0.4165062353480806 1105 20-21 0.2914422521849076 1105 22-23 0.18156285248866055 1105 24-25 0.2755170561539231 1105 26-27 0.36317450449959665 1105 28-29 0.30559242037193 1105 30-31 0.2662993416534718 1105 32-33 0.28714482563908206 1105 34-35 0.42880045314041837 1105 36-37 0.25071383150753945 1105 38-39 0.24035123892943488 1105 40-41 0.20828477849632776 1105 42-43 0.22839846397226893 1105 44-45 0.24822543599830738 1105 46-47 0.3206315770470809 1105 48-49 0.5725411334554593 1105 50-51 0.41192226217316374 1105 52-53 0.36547589158782756 1105 54-55 0.25080210763922395 1105 56-57 0.228830796182109 1105 58-59 0.4286141015868523 1105 60-61 0.39411042583896005 1105 62-63 0.5509117003497934 1105 64-65 0.35756952396726405 1105 66-67 0.48736416512961256 1105 68-69 0.4878331850002908 1105 70-71 0.45762179519964974 1105 72-73 0.528715173663258 1105 74-75 0.4650231427273894 1105 76-77 0.44387581607323057 1105 78-79 0.5515341006467587 1105 80-81 0.6803056475790683 1105 82-83 0.5991817065605964 1105 84-85 0.2869483185967141 1105 86-87 0.3523550468714838 1105 88-89 0.4556487431734233 1105 90-91 0.5402011154908593 1105 92-93 0.7062662535979278 1105 94-95 0.6107203954030922 1105 96-97 0.9021538714133719 1105 98-99 0.6524220729994994 1105 100-101 1.117690050383377 1106 1 0.15133417474747546 1106 2 0.11099786597465311 1106 3 0.09198626421845546 1106 4 0.16227494957691135 1106 5 0.12377190482883549 1106 6 0.13875387373028758 1106 7 0.06465566202339801 1106 8 0.13119130037102877 1106 9 0.17105069570537523 1106 10-11 0.2077913934877884 1106 12-13 0.10139728139660775 1106 14-15 0.259439916973804 1106 16-17 0.25237764580007394 1106 18-19 0.2786420077740317 1106 20-21 0.08839260322404385 1106 22-23 0.17491587757887572 1106 24-25 0.12274393092665292 1106 26-27 0.2724574895909271 1106 28-29 0.18836224499221998 1106 30-31 0.1850263143255617 1106 32-33 0.21858797408248876 1106 34-35 0.24968826047447834 1106 36-37 0.25356063299561526 1106 38-39 0.2821026864413909 1106 40-41 0.42288823014802546 1106 42-43 0.30634738847788157 1106 44-45 0.15639796264091643 1106 46-47 0.24073988170989224 1106 48-49 0.24885732041526154 1106 50-51 0.1465812087760554 1106 52-53 0.15295618584532633 1106 54-55 0.2547631716654095 1106 56-57 0.19294268116434665 1106 58-59 0.22598927990354412 1106 60-61 0.22120863288211012 1106 62-63 0.3103069620207961 1106 64-65 0.23134726445005072 1106 66-67 0.1435553765642581 1106 68-69 0.22202848023679422 1106 70-71 0.2273185309552872 1106 72-73 0.08173847371080711 1106 74-75 0.08738044741816964 1106 76-77 -0.02775955076965886 1106 78-79 0.14858727398457106 1106 80-81 0.34748821589883505 1106 82-83 0.23891083301859695 1106 84-85 0.08740301916452964 1106 86-87 0.04019303741720037 1106 88-89 -0.023562691471759933 1106 90-91 0.13913115940571785 1106 92-93 0.29566067613156477 1106 94-95 0.4078894815824974 1106 96-97 0.4341389907107356 1106 98-99 0.5961215499372052 1106 100-101 0.6523033479135165 1107 1 0.15512548987395292 1107 2 0.09090261837737046 1107 3 0.11838671510611931 1107 4 0.1433487678964056 1107 5 0.061932106063224523 1107 6 0.08311620311394563 1107 7 0.1637454512633667 1107 8 0.12981674491010153 1107 9 0.10436438309300655 1107 10-11 0.05851502282517629 1107 12-13 0.1555046826102071 1107 14-15 0.1858358968671041 1107 16-17 0.2092536937582068 1107 18-19 0.2663645030646151 1107 20-21 0.15306707849338608 1107 22-23 0.024256692229286614 1107 24-25 0.17944109506081674 1107 26-27 0.23806287874693766 1107 28-29 0.2644789419096796 1107 30-31 0.28129332034731647 1107 32-33 0.17080207436534778 1107 34-35 0.19607233827242254 1107 36-37 0.28377728681091696 1107 38-39 0.22428083596509651 1107 40-41 0.39994387993217373 1107 42-43 0.24510434632521338 1107 44-45 0.2978243243725558 1107 46-47 0.47252041447181625 1107 48-49 0.4633794845439283 1107 50-51 0.35954986754602203 1107 52-53 0.3585615794062562 1107 54-55 0.3134737148647915 1107 56-57 0.3137200967842375 1107 58-59 0.32747468519037426 1107 60-61 0.3433887954545156 1107 62-63 0.4783132751529422 1107 64-65 0.42243844476392667 1107 66-67 0.37630719430979553 1107 68-69 0.317774824648275 1107 70-71 0.22073064188792557 1107 72-73 0.3268272625933122 1107 74-75 0.33670632938788003 1107 76-77 0.2876349638268181 1107 78-79 0.30740811639479304 1107 80-81 0.5687243599459038 1107 82-83 0.5757931007245602 1107 84-85 0.579400379736132 1107 86-87 0.45678487549584545 1107 88-89 0.3568567098246511 1107 90-91 0.37291626139174383 1107 92-93 0.4362153040842607 1107 94-95 0.5149019609426944 1107 96-97 0.5482863401488984 1107 98-99 0.8545063712857406 1107 100-101 1.1740309489475287 1108 1 0.13120879171223265 1108 2 0.05114053709482391 1108 3 0.07128453765314902 1108 4 0.030220987831462764 1108 5 -0.024168483260247342 1108 6 0.036226468539908296 1108 7 0.08159971761178753 1108 8 0.1588922138366513 1108 9 0.10261573157526271 1108 10-11 0.17929226089035666 1108 12-13 0.11370027240854341 1108 14-15 0.18755577557130465 1108 16-17 0.1367109407105005 1108 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-1.1087973432567289 2316 78-79 -0.8631071147798046 2316 80-81 -0.8899132850270881 2316 82-83 -1.032664027759619 2316 84-85 -1.2127587324356206 2316 86-87 -0.9758366165488503 2316 88-89 -1.1114708516843876 2316 90-91 -0.9855815101421754 2316 92-93 -1.1488252929544664 2316 94-95 -1.216835506427838 2316 96-97 -1.3117144573750963 2316 98-99 -1.5133745767862443 2316 100-101 -1.8350485549925502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 10.0 5 49.0 6 85.0 7 217.0 8 555.0 9 1073.0 10 1903.0 11 3046.0 12 4558.0 13 6327.0 14 8198.0 15 11004.0 16 14723.0 17 19756.0 18 18237.0 19 12012.0 20 10600.0 21 11236.0 22 11625.0 23 12909.0 24 14878.0 25 18406.0 26 22416.0 27 28103.0 28 35350.0 29 44160.0 30 54780.0 31 69371.0 32 88120.0 33 115482.0 34 157369.0 35 222611.0 36 339418.0 37 507780.0 38 367871.0 39 27861.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.678695319764984 19.529389707400743 14.957799927392971 22.8341150454413 2 15.504307095821554 20.75513988943242 39.844117835598695 23.896435179147336 3 19.4395824721416 24.74555514537653 29.92614567053586 25.888716711946007 4 13.235557406334172 17.13558706889273 37.062637509830424 32.56621801494268 5 13.829775006211268 37.29904006068917 34.22395400920771 14.647230923891852 6 31.740072993733293 36.53713874467754 17.343482550819765 14.379305710769405 7 28.07566557270374 32.81583758894343 21.051168111923722 18.057328726429105 8 26.122446633942086 35.15352025010256 19.757642415583312 18.966390700372042 9 26.086418355943476 16.869677540280943 20.440809296798744 36.60309480697684 10-11 24.686708704078313 26.889230064647553 27.472998684415273 20.951062546858864 12-13 25.761247579139646 24.161589914544347 28.080256079614202 21.996906426701806 14-15 22.686918242774894 25.539075872459012 26.364432883959772 25.409573000806322 16-17 21.917271079977947 28.731503449653882 27.04199749702822 22.309227973339947 18-19 21.880309876284233 27.236986568741507 29.431505179377805 21.45119837559646 20-21 22.614857391428895 26.368947678803362 29.583575368711614 21.432619561056132 22-23 22.309837001881426 26.364742329292273 29.508684804289086 21.81673586453721 24-25 22.2036568461255 26.467052155493214 29.60101486465487 21.728276133726414 26-27 22.112383463486402 26.626449190787426 29.469988753076986 21.791178592649178 28-29 21.85272943866055 26.741241650490995 29.700566054544343 21.705462856304113 30-31 22.068076655278766 26.52824005110293 29.559458342555462 21.844224951062845 32-33 21.95998434191043 26.61537043516625 29.541757742122936 21.882887480800385 34-35 22.342616000924856 26.527033559824144 29.392139746871702 21.738210692379294 36-37 22.435569511619793 26.399577132432622 29.324234282073537 21.84061907387404 38-39 22.105550990832313 26.61156283081809 29.396441903230965 21.886444275118635 40-41 22.30568801101212 26.56547907341332 29.318111130539464 21.810721785035092 42-43 22.200473918982844 26.676510506235662 29.05410722416987 22.06890835061163 44-45 22.26582626830635 26.76385844968386 28.908797326286546 22.061517955723254 46-47 22.440752998406552 26.915977421630082 28.558096952070365 22.085172627892998 48-49 22.54974013977834 27.081561827016397 28.275484327156203 22.093213706049053 50-51 22.50167160503924 27.13024372830367 28.181083730558264 22.187000936098823 52-53 22.574161613366265 27.271929525966016 28.02275189838611 22.131156962281608 54-55 22.519203008027343 27.12158121693724 28.001832826020117 22.357382949015296 56-57 22.442679655247947 27.41136016059488 27.90101806316682 22.24494212099035 58-59 22.633270677356496 27.211253496769356 27.93231297809811 22.223162847776038 60-61 22.533256531948904 27.040305696623335 28.34890580130427 22.077531970123495 62-63 22.431977224803394 27.04360088589856 28.325545618913317 22.19887627038473 64-65 22.446699632396015 27.16058524853611 28.200332015146255 22.192383103921625 66-67 22.356657671311993 27.29865056417871 27.96155419747239 22.383137567036908 68-69 22.262838500193958 27.19333979490229 28.187220460701763 22.356601244201997 70-71 22.343732662197834 27.05738235050974 28.132519494597126 22.4663654926953 72-73 22.467050237762646 27.04600664643365 27.98870110700944 22.498242008794268 74-75 22.365476167581917 27.400533300795043 27.77039108503922 22.463599446583824 76-77 22.449445583545312 27.287979140749542 27.794319054932366 22.46825622077278 78-79 22.474615230044055 27.266933514483902 27.724349019613914 22.534102235858132 80-81 22.52912104185915 27.35965536600784 27.610350513458677 22.500873078674335 82-83 22.47024681203607 27.347380143409474 27.752024825987974 22.430348218566483 84-85 22.425604000558778 27.276396455657704 27.65730627156198 22.640693272221544 86-87 22.377959181508253 27.22325419784697 27.710387460921478 22.688399159723303 88-89 22.555334322398796 27.219517715647807 27.580868479638987 22.644279482314413 90-91 22.450392415647823 27.238510953532334 27.7141575767596 22.596939054060236 92-93 22.319457230607366 27.13703291517515 27.736298421490623 22.807211432726852 94-95 22.26916973847979 27.1066869044943 27.75040277830273 22.873740578723183 96-97 22.233401580960745 27.128951324330362 27.729962196723363 22.90768489798553 98-99 22.21959684524486 27.09467138851399 27.52325374241471 23.162478023826438 100-101 22.020543751621837 27.26450080213112 27.573815292787806 23.141140153459244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 558.0 1 396.5 2 296.0 3 387.5 4 343.0 5 310.5 6 551.0 7 1200.0 8 2008.5 9 2423.5 10 2290.0 11 2131.0 12 2235.5 13 2385.0 14 2623.0 15 2822.5 16 2967.5 17 3156.0 18 3437.5 19 3725.0 20 4117.0 21 4706.5 22 5577.0 23 6826.0 24 8561.0 25 10787.5 26 13604.0 27 16934.5 28 19881.0 29 23043.0 30 27253.5 31 31100.0 32 34689.0 33 38906.5 34 44168.5 35 48560.0 36 53205.0 37 58280.5 38 61367.5 39 63939.5 40 67168.5 41 70841.5 42 74014.0 43 77977.0 44 82234.5 45 85354.5 46 86380.5 47 87777.0 48 92321.0 49 90501.5 50 83511.0 51 79165.5 52 76449.0 53 74911.0 54 72092.5 55 67103.0 56 61289.5 57 55518.0 58 50700.5 59 45766.5 60 38986.0 61 31382.0 62 24646.0 63 19322.5 64 14794.0 65 11192.0 66 8477.5 67 6504.5 68 4604.0 69 3045.0 70 2124.5 71 1433.5 72 951.0 73 679.0 74 515.0 75 372.0 76 220.5 77 114.0 78 72.5 79 38.0 80 23.5 81 17.5 82 13.5 83 10.0 84 5.5 85 3.5 86 4.5 87 3.5 88 1.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02754063459280531 2 0.006498351982572039 3 0.010034870068325529 4 4.420647607191863E-5 5 0.004155408750760352 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.326194282157559E-4 14-15 0.0 16-17 4.420647607191863E-5 18-19 0.004973228558090846 20-21 0.002033497899308257 22-23 0.0 24-25 0.005989977507744975 26-27 0.007073036171506981 28-29 0.010056973306361489 30-31 0.035077838763067436 32-33 9.94645711618169E-4 34-35 0.006034183983816893 36-37 0.018168861665558558 38-39 0.008863398452419687 40-41 0.010631657495296432 42-43 0.005393190080774073 44-45 0.003956479608436718 46-47 0.0015693299005531114 48-49 0.0013482975201935183 50-51 0.0028513177066387518 52-53 0.0026081820882431993 54-55 0.001392503996265437 56-57 9.94645711618169E-4 58-59 0.0010609554257260472 60-61 0.0 62-63 8.841295214383726E-5 64-65 0.007382481504010411 66-67 8.178198073304947E-4 68-69 8.620262834024133E-4 70-71 0.0033154857053938978 72-73 0.013151426631395792 74-75 0.007139345885614859 76-77 0.004155408750760352 78-79 0.012090471205669745 80-81 8.841295214383726E-5 82-83 0.005636325699169626 84-85 0.0012377813300137217 86-87 0.0 88-89 0.00194508494716442 90-91 0.0014809169484092742 92-93 4.1996152268322705E-4 94-95 5.083744748270643E-4 96-97 2.8734209446747106E-4 98-99 1.326194282157559E-4 100-101 5.746841889349421E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2262112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.699819858999405 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61596053405782 44.20727121139343 2 13.46112311877513 15.534087581062748 3 4.256686104427727 7.368300642653576 4 1.9266078388250063 4.4465970095655605 5 1.0067730036348683 2.904531047431783 6 0.5876854400696394 2.0345606415464994 7 0.38436542705070975 1.552447113059733 8 0.2957043154251994 1.3649668585250196 9 0.21368064985594482 1.109640150363775 >10 1.1447041843658106 11.97704353619042 >50 0.07570362946780908 3.0053608936291667 >100 0.029392114875923064 2.906970540614856 >500 0.0011525994059990025 0.4446066098293167 >1k 3.07359841599677E-4 0.43618267127046106 >5k 1.536799207998385E-4 0.707433492863537 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8948 0.3955595478915279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7008 0.3097989843120058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4739 0.2094944901048224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.420647607191863E-5 0.0 8 0.0 0.0 0.0 8.841295214383726E-5 0.0 9 0.0 0.0 0.0 1.326194282157559E-4 0.0 10-11 0.0 0.0 0.0 1.326194282157559E-4 0.0 12-13 0.0 0.0 0.0 1.326194282157559E-4 0.0 14-15 0.0 0.0 0.0 1.326194282157559E-4 0.0 16-17 0.0 0.0 0.0 1.5472266625171522E-4 0.0 18-19 0.0 0.0 0.0 2.2103238035959315E-4 0.0 20-21 0.0 0.0 0.0 3.3154857053938974E-4 0.0 22-23 0.0 0.0 0.0 4.86271236791105E-4 0.0 24-25 0.0 0.0 0.0 8.620262834024133E-4 2.2103238035959316E-5 26-27 0.0 0.0 0.0 0.0011493683778698845 4.420647607191863E-5 28-29 0.0 0.0 0.0 0.002033497899308257 4.420647607191863E-5 30-31 0.0 0.0 0.0 0.004265924940940148 6.630971410787795E-5 32-33 0.0 0.0 0.0 0.006984623219363144 8.841295214383726E-5 34-35 0.0 0.0 0.0 0.009614908545642302 8.841295214383726E-5 36-37 0.0 0.0 0.0 0.011847335587274193 8.841295214383726E-5 38-39 0.0 0.0 0.0 0.015030201864452334 8.841295214383726E-5 40-41 0.0 0.0 0.0 0.019185610615212685 8.841295214383726E-5 42-43 0.0 0.0 0.0 0.02524189783706554 8.841295214383726E-5 44-45 0.0 0.0 0.0 0.030701397631947493 8.841295214383726E-5 46-47 0.0 0.0 0.0 0.036890304282016104 8.841295214383726E-5 48-49 0.0 0.0 0.0 0.04243821702904189 8.841295214383726E-5 50-51 0.0 0.0 0.0 0.04962176939072867 8.841295214383726E-5 52-53 0.0 0.0 0.0 0.0692715480046965 8.841295214383726E-5 54-55 0.0 0.0 0.0 0.09418189727122264 8.841295214383726E-5 56-57 0.0 0.0 0.0 0.11542310902377954 8.841295214383726E-5 58-59 0.0 0.0 0.0 0.15383853673027684 8.841295214383726E-5 60-61 0.0 0.0 0.0 0.20131629203151746 8.841295214383726E-5 62-63 0.0 0.0 0.0 0.2322387220438245 8.841295214383726E-5 64-65 0.0 0.0 0.0 0.25845316235447224 8.841295214383726E-5 66-67 0.0 0.0 0.0 0.32973610502044104 8.841295214383726E-5 68-69 0.0 0.0 0.0 0.40851204538060004 8.841295214383726E-5 70-71 0.0 0.0 0.0 0.4396997142493386 8.841295214383726E-5 72-73 0.0 0.0 0.0 0.49882587599552985 8.841295214383726E-5 74-75 0.0 0.0 0.0 0.590001732893862 8.841295214383726E-5 76-77 0.0 0.0 0.0 0.6832552941675744 8.841295214383726E-5 78-79 0.0 0.0 0.0 0.7666950177533208 8.841295214383726E-5 80-81 0.0 0.0 0.0 0.8777637888840164 8.841295214383726E-5 82-83 0.0 0.0 0.0 1.0051226464472138 8.841295214383726E-5 84-85 0.0 0.0 0.0 1.1464507504491377 8.841295214383726E-5 86-87 0.0 0.0 0.0 1.3569398862655784 8.841295214383726E-5 88-89 0.0 0.0 0.0 1.6153930486200507 8.841295214383726E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7275 0.0 32.008507 1 GTATCAA 10645 0.0 31.696772 1 ATCAACG 13405 0.0 24.98231 3 TATCAAC 13445 0.0 24.909637 2 GTACATG 8430 0.0 24.52246 1 TCAACGC 13620 0.0 24.48007 4 CAACGCA 13785 0.0 24.187056 5 AACGCAG 14135 0.0 23.654312 6 ACATGGG 8325 0.0 23.337233 3 TACATGG 8580 0.0 23.198816 2 ACGCAGA 15920 0.0 20.913082 7 CGCAGAG 15755 0.0 20.8005 8 GAGTACT 8645 0.0 20.273262 12-13 AGTACTT 9300 0.0 19.585953 12-13 AGAGTAC 13560 0.0 19.57921 10-11 GCAGAGT 16685 0.0 19.12873 9 GTACTTT 9195 0.0 18.88066 14-15 TAGACCG 155 9.634545E-4 18.385365 5 CATGGGG 4145 0.0 18.21903 4 CATGGGA 4965 0.0 18.079868 4 >>END_MODULE