FastQCFastQC Report
Thu 26 May 2016
SRR936857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268070
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.3262244992438505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54980.24240874399820111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33510.14774676266605527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.033.6304741
GTATCAA102700.033.2695541
ATCAACG122200.027.7673663
TATCAAC122900.027.6478822
TCAACGC122900.027.6092154
CAACGCA124950.027.0801725
AACGCAG127650.026.542276
TACATGG88250.023.9098012
GTACATG90450.023.9051441
ACGCAGA143300.023.609357
GAGTACT67900.023.19564812-13
CGCAGAG144250.023.190858
ACATGGG89100.022.1882653
GCAGAGT151600.021.5317349
GTACTTT73700.020.9143414-15
AGAGTAC123100.019.37592110-11
AGTACTT74400.019.18952812-13
CATGGGG42550.018.31694
CAGAGTA147400.018.05124710-11
ACTTTTT91250.017.41188616-17
Produced by FastQC (version 0.11.5)