##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936857_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268070 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60333411226285 33.0 31.0 34.0 30.0 34.0 2 31.719074808096753 33.0 31.0 34.0 30.0 34.0 3 31.72709219733077 33.0 31.0 34.0 30.0 34.0 4 35.29703712848369 37.0 35.0 37.0 33.0 37.0 5 35.2738173865886 37.0 35.0 37.0 33.0 37.0 6 35.389184637158465 37.0 35.0 37.0 33.0 37.0 7 35.325857226628806 37.0 35.0 37.0 33.0 37.0 8 35.28280299990741 37.0 35.0 37.0 32.0 37.0 9 36.854861181533195 39.0 37.0 39.0 33.0 39.0 10-11 36.7267430017592 39.0 37.0 39.0 32.0 39.0 12-13 36.61054376628587 39.0 37.0 39.0 32.0 39.0 14-15 37.76160325739505 40.0 37.5 41.0 32.0 41.0 16-17 37.671212308262085 40.0 37.0 41.0 32.0 41.0 18-19 37.59412011093132 40.0 37.0 41.0 32.0 41.0 20-21 37.46308403179796 40.0 37.0 41.0 32.0 41.0 22-23 37.33892869267704 40.0 37.0 41.0 32.0 41.0 24-25 37.464169756665356 40.0 37.0 41.0 32.0 41.0 26-27 37.41302605298778 40.0 37.0 41.0 32.0 41.0 28-29 37.32401050232136 40.0 37.0 41.0 31.0 41.0 30-31 37.15033993659807 40.0 37.0 41.0 31.0 41.0 32-33 36.961206223793795 39.0 36.0 41.0 30.5 41.0 34-35 36.77622163337111 39.0 36.0 41.0 30.0 41.0 36-37 36.58656059998148 39.0 35.5 41.0 30.0 41.0 38-39 36.421729488066944 39.0 35.0 41.0 30.0 41.0 40-41 36.2379505482635 39.0 35.0 41.0 30.0 41.0 42-43 36.01921303134383 39.0 35.0 40.0 29.0 41.0 44-45 35.76902917458456 39.0 35.0 40.0 28.0 41.0 46-47 35.443609985582455 38.0 35.0 40.0 26.5 41.0 48-49 35.205990114943546 38.5 34.5 40.0 26.0 41.0 50-51 34.50619248964979 38.0 34.0 39.5 25.0 40.5 52-53 34.69896696310079 38.0 34.0 39.5 26.0 40.5 54-55 35.10848673100918 39.0 35.0 40.0 26.0 41.0 56-57 35.00668696292443 38.5 34.5 40.0 26.0 41.0 58-59 34.74250882909257 38.0 34.0 40.0 25.5 41.0 60-61 34.44797118254728 38.0 34.0 40.0 24.5 41.0 62-63 34.09938670323226 37.5 34.0 40.0 23.0 41.0 64-65 33.755004916074014 37.0 33.0 40.0 22.5 41.0 66-67 33.37240407042111 36.5 33.0 39.0 22.0 41.0 68-69 32.966517788251686 36.0 32.0 39.0 21.5 41.0 70-71 32.56424052167702 35.5 32.0 39.0 20.0 40.0 72-73 32.15343926774747 35.0 32.0 38.0 19.5 40.0 74-75 31.69073022437579 35.0 31.0 37.0 18.0 39.5 76-77 31.24277755977549 35.0 31.0 37.0 15.0 39.0 78-79 30.79819979101174 35.0 31.0 36.0 11.0 39.0 80-81 30.353730704960604 34.0 30.0 36.0 7.5 37.5 82-83 29.9278703920073 34.0 30.0 35.0 6.5 37.0 84-85 29.51862067749232 34.0 29.0 35.0 2.0 37.0 86-87 29.154444527726216 34.0 29.0 35.0 2.0 36.0 88-89 28.78861013989868 34.0 29.0 35.0 2.0 36.0 90-91 28.386238519975134 34.0 29.0 35.0 2.0 35.5 92-93 27.995560322212278 34.0 27.5 35.0 2.0 35.0 94-95 27.54808736061938 34.0 27.0 35.0 2.0 35.0 96-97 26.99098837337472 33.5 25.5 35.0 2.0 35.0 98-99 26.294792047864483 33.0 25.0 35.0 2.0 35.0 100-101 23.670428602291818 30.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3700223397847786 1101 2 0.5346946295329928 1101 3 0.3896986849892734 1101 4 0.4096699875284173 1101 5 0.41261861218383444 1101 6 0.40988471915765956 1101 7 0.3816132433438426 1101 8 0.3045457513705898 1101 9 0.4177316119221075 1101 10-11 0.4892981220918884 1101 12-13 0.5451404439849483 1101 14-15 0.7603744754337924 1101 16-17 0.7310130582549519 1101 18-19 0.7765355601769315 1101 20-21 0.7028438569850621 1101 22-23 0.8043281495650731 1101 24-25 0.728379819449195 1101 26-27 0.8179258912835365 1101 28-29 0.7256072660539061 1101 30-31 0.6801928062550004 1101 32-33 0.7900298411752402 1101 34-35 0.6600173466257573 1101 36-37 0.7912000192499491 1101 38-39 0.832565172109625 1101 40-41 0.8450701660391786 1101 42-43 0.7843598796323192 1101 44-45 0.8729017885209558 1101 46-47 0.7520082307145302 1101 48-49 0.8298775731981323 1101 50-51 0.9255938118121563 1101 52-53 0.8961546552524311 1101 54-55 0.9425497429258058 1101 56-57 0.7588427536714732 1101 58-59 0.8208809797897132 1101 60-61 1.0761523145723046 1101 62-63 1.0558539670020863 1101 64-65 1.060412647057973 1101 66-67 1.0896398881148457 1101 68-69 0.8469521532710118 1101 70-71 0.9215011719176758 1101 72-73 0.9536836490661287 1101 74-75 0.9674886372986542 1101 76-77 0.951106564876735 1101 78-79 1.0116054254537374 1101 80-81 0.9863363670292955 1101 82-83 1.0915225442087753 1101 84-85 0.9658110493627134 1101 86-87 1.014011840751781 1101 88-89 1.0627393771847942 1101 90-91 0.9067908216736917 1101 92-93 0.9225589820311875 1101 94-95 1.0323005715316143 1101 96-97 1.0319624168198942 1101 98-99 0.9916572801652812 1101 100-101 1.2332080284041211 1102 1 0.35368923053624357 1102 2 0.4336788469733648 1102 3 0.349845306345582 1102 4 0.3283627713811583 1102 5 0.3825486936150142 1102 6 0.3635394599902284 1102 7 0.32026478737034125 1102 8 0.4109393635147498 1102 9 0.39791018078090445 1102 10-11 0.38577536514988253 1102 12-13 0.39986277413792237 1102 14-15 0.42478785638000716 1102 16-17 0.6380021397745352 1102 18-19 0.6474610715117137 1102 20-21 0.6954449469828674 1102 22-23 0.5445434559958997 1102 24-25 0.3345999200612084 1102 26-27 0.5953621967213962 1102 28-29 0.5349319690387517 1102 30-31 0.517084565073965 1102 32-33 0.7234820011265626 1102 34-35 0.5896948084234097 1102 36-37 0.4893352842353593 1102 38-39 0.6934483951480104 1102 40-41 0.7056536499616115 1102 42-43 0.7137544600695094 1102 44-45 0.46162405288756503 1102 46-47 0.6400785933412934 1102 48-49 0.4468935243353087 1102 50-51 0.5285025969641737 1102 52-53 0.5905205672261644 1102 54-55 0.7491412281146239 1102 56-57 0.88685273675204 1102 58-59 0.7887088785527183 1102 60-61 0.6636902188243639 1102 62-63 0.8813172983001536 1102 64-65 0.8907151820943469 1102 66-67 0.815701566941712 1102 68-69 0.8178427156005128 1102 70-71 0.8477952616297131 1102 72-73 0.7095138626867836 1102 74-75 0.7421606820004847 1102 76-77 0.8414369974426315 1102 78-79 0.7047052583152613 1102 80-81 0.6243482414677963 1102 82-83 0.5785987104262453 1102 84-85 0.6998120114882695 1102 86-87 0.7043364142197284 1102 88-89 0.7889671665779794 1102 90-91 0.6642846079461329 1102 92-93 0.6091313686165236 1102 94-95 0.8078413045972788 1102 96-97 0.778222359297942 1102 98-99 0.6003909337959676 1102 100-101 0.5975592206791589 1103 1 0.3070293336404539 1103 2 0.49466250067262507 1103 3 0.4425324560623629 1103 4 0.41775310259070153 1103 5 0.4657983034545481 1103 6 0.38629787202963684 1103 7 0.2890405671670635 1103 8 0.3719766361957326 1103 9 0.38950907485195785 1103 10-11 0.5390115253118779 1103 12-13 0.6747846671575672 1103 14-15 0.687698198852523 1103 16-17 0.7161693291513842 1103 18-19 0.8569411471174746 1103 20-21 0.8168679767848346 1103 22-23 0.8030693234102344 1103 24-25 0.6339298325634175 1103 26-27 0.7441440872847522 1103 28-29 0.7861695490553444 1103 30-31 0.8750359006059796 1103 32-33 0.9679114682018124 1103 34-35 0.7777970858821703 1103 36-37 1.0301974415157105 1103 38-39 0.9952301029874633 1103 40-41 0.7967972320091334 1103 42-43 0.5789533445550603 1103 44-45 0.6225193401085747 1103 46-47 0.7279286519028076 1103 48-49 0.8359052276515939 1103 50-51 0.6431624459323828 1103 52-53 0.7871743234005848 1103 54-55 0.8090294370309508 1103 56-57 0.9398067232784015 1103 58-59 0.943983758209562 1103 60-61 0.9379444207252874 1103 62-63 1.0318416559956773 1103 64-65 0.9527020199823042 1103 66-67 0.7222730374497672 1103 68-69 0.8591098609438959 1103 70-71 0.7845128813063837 1103 72-73 0.7212462350721083 1103 74-75 0.7848066439519776 1103 76-77 0.7425600005848842 1103 78-79 0.7786536223372202 1103 80-81 0.9502060702884485 1103 82-83 0.9516319571903828 1103 84-85 0.8501404651947198 1103 86-87 0.8005510339293487 1103 88-89 0.879296878383439 1103 90-91 0.8245376655760523 1103 92-93 0.8360388114703703 1103 94-95 0.9812765439506173 1103 96-97 1.0129372051658336 1103 98-99 0.8061659078102359 1103 100-101 1.0019730513809648 1104 1 0.45249360076918776 1104 2 0.475360974369778 1104 3 0.40579253372138524 1104 4 0.3336400338012808 1104 5 0.4557500953878417 1104 6 0.3907915729566227 1104 7 0.3389413489040436 1104 8 0.4109599778210793 1104 9 0.4456322999315887 1104 10-11 0.5602239324555924 1104 12-13 0.6120561363312831 1104 14-15 0.6574409176044966 1104 16-17 0.5510406885273653 1104 18-19 0.5875146687081667 1104 20-21 0.7973105417056061 1104 22-23 0.7163740063978778 1104 24-25 0.6494216063114706 1104 26-27 0.5583182882489126 1104 28-29 0.5497021195231895 1104 30-31 0.4379771645379975 1104 32-33 0.6749868454885473 1104 34-35 0.6129889745747121 1104 36-37 0.4888714623429635 1104 38-39 0.5577856451995444 1104 40-41 0.6074058659995387 1104 42-43 0.590522136837194 1104 44-45 0.7078413276762703 1104 46-47 0.6458196776538045 1104 48-49 0.5910905971038147 1104 50-51 0.49007752996767806 1104 52-53 0.48143996528842337 1104 54-55 0.4362469795060875 1104 56-57 0.6494274636124828 1104 58-59 0.7088490558357634 1104 60-61 0.6719565556621276 1104 62-63 0.7786889742432592 1104 64-65 0.7305523290743565 1104 66-67 0.5987462999864377 1104 68-69 0.5484137318858089 1104 70-71 0.5750679889024397 1104 72-73 0.6320452995039361 1104 74-75 0.579699333824852 1104 76-77 0.5651434497205088 1104 78-79 0.827556216880506 1104 80-81 0.6199367799134805 1104 82-83 0.4662816623949091 1104 84-85 0.4112220300411451 1104 86-87 0.525816521414125 1104 88-89 0.43803952279319347 1104 90-91 0.4940310519782898 1104 92-93 0.3237366046503318 1104 94-95 0.4169322136881881 1104 96-97 0.4101745341072629 1104 98-99 0.6848168253647167 1104 100-101 0.8784394515593874 1105 1 0.31030236759434615 1105 2 0.34416730465152057 1105 3 0.4175825651835474 1105 4 0.4306972570880063 1105 5 0.43354679777471006 1105 6 0.4254070485557051 1105 7 0.29430292721644236 1105 8 0.4334069297762255 1105 9 0.46597270633757404 1105 10-11 0.4536984906842534 1105 12-13 0.47825855868124023 1105 14-15 0.5071026402170347 1105 16-17 0.7226383986496643 1105 18-19 0.6656100250822092 1105 20-21 0.6315114500868404 1105 22-23 0.6623643548472344 1105 24-25 0.5658812850662258 1105 26-27 0.43016201828936573 1105 28-29 0.45015631014131685 1105 30-31 0.2553126978571427 1105 32-33 0.4959133233822328 1105 34-35 0.3168424981003781 1105 36-37 0.5552631307838212 1105 38-39 0.3655683906872156 1105 40-41 0.42993620451348846 1105 42-43 0.39918251107133074 1105 44-45 0.3500538510363427 1105 46-47 0.35467284634304264 1105 48-49 0.3299694322941207 1105 50-51 0.32342564815186137 1105 52-53 0.38466483695592046 1105 54-55 0.4850553205275432 1105 56-57 0.5191433579185443 1105 58-59 0.6003004550740414 1105 60-61 0.36404429488101897 1105 62-63 0.46499169702139653 1105 64-65 0.6287964801873613 1105 66-67 0.44604244646291136 1105 68-69 0.5182348943299218 1105 70-71 0.47354337508274824 1105 72-73 0.3485179889274477 1105 74-75 0.37118562531114563 1105 76-77 0.3077941960597812 1105 78-79 0.5074393574193827 1105 80-81 0.35617326655980364 1105 82-83 0.37181829334137717 1105 84-85 0.5123598721625946 1105 86-87 0.413445741010559 1105 88-89 0.36366048652890726 1105 90-91 0.31400301990107593 1105 92-93 0.3578600404131116 1105 94-95 0.47500237667638245 1105 96-97 0.5677236789514879 1105 98-99 0.4386413705827046 1105 100-101 0.5064064754280508 1106 1 0.21316673045640044 1106 2 0.3416313237387243 1106 3 0.3640511764870702 1106 4 0.4117999021019898 1106 5 0.42758717849253003 1106 6 0.33686193451994484 1106 7 0.3206863644518734 1106 8 0.4559267481399658 1106 9 0.5498088745307044 1106 10-11 0.5259912637158806 1106 12-13 0.5333414238137522 1106 14-15 0.7516394420847092 1106 16-17 0.5780695282322625 1106 18-19 0.42686287918314036 1106 20-21 0.653825388361291 1106 22-23 0.5563380329816212 1106 24-25 0.6414617258163773 1106 26-27 0.6150038562020868 1106 28-29 0.6113507994817269 1106 30-31 0.601939727796335 1106 32-33 0.5545429701150937 1106 34-35 0.6831859137857421 1106 36-37 0.5773538719521341 1106 38-39 0.6003165884347226 1106 40-41 0.6481594785841409 1106 42-43 0.5000611893339908 1106 44-45 0.7208252920686888 1106 46-47 0.7678915025425326 1106 48-49 0.6160433438875472 1106 50-51 0.5305018943357069 1106 52-53 0.6167365935427966 1106 54-55 0.4611418029924579 1106 56-57 0.7078629248648483 1106 58-59 0.5936975406842393 1106 60-61 0.6409889317367572 1106 62-63 0.7871427270632054 1106 64-65 0.5775951923143978 1106 66-67 0.8670653049251626 1106 68-69 0.7785673810535414 1106 70-71 0.589042746539171 1106 72-73 0.4867950098874516 1106 74-75 0.6754892246851689 1106 76-77 0.6607656678779357 1106 78-79 0.6823425103991916 1106 80-81 0.6187447794257075 1106 82-83 0.7519563163794238 1106 84-85 0.5925158535884876 1106 86-87 0.7187498791362152 1106 88-89 0.8311679905695435 1106 90-91 0.8743066449299413 1106 92-93 0.8398362327217903 1106 94-95 0.8304771194866163 1106 96-97 0.8992117879332469 1106 98-99 0.8576599446867128 1106 100-101 0.6282656816673082 1107 1 0.2137081149968374 1107 2 0.19610822289228835 1107 3 0.23694684987796677 1107 4 0.24350886908923286 1107 5 0.31307360558856345 1107 6 0.24458852362715788 1107 7 0.17965281988284687 1107 8 0.15248507408111323 1107 9 0.35189846994946095 1107 10-11 0.2864289236583204 1107 12-13 0.4114010973663227 1107 14-15 0.42438376180152915 1107 16-17 0.5423357022283639 1107 18-19 0.42339067911974837 1107 20-21 0.5951477068060242 1107 22-23 0.4959118601767827 1107 24-25 0.4817938104553079 1107 26-27 0.3456798317047003 1107 28-29 0.34678489628426235 1107 30-31 0.44260951300813645 1107 32-33 0.274732146253335 1107 34-35 0.2585951430626281 1107 36-37 0.418119702397739 1107 38-39 0.37096684551259784 1107 40-41 0.3819588918892194 1107 42-43 0.5211903962322282 1107 44-45 0.4546269389838571 1107 46-47 0.5303520686882379 1107 48-49 0.45673748608554376 1107 50-51 0.4872549485315929 1107 52-53 0.49648232362484634 1107 54-55 0.5882070946062115 1107 56-57 0.5883570206888464 1107 58-59 0.6069885843332443 1107 60-61 0.5820331125443019 1107 62-63 0.5301871588859868 1107 64-65 0.6035403816500846 1107 66-67 0.5640549303381164 1107 68-69 0.48795184065817665 1107 70-71 0.44190059746591004 1107 72-73 0.47153339032868047 1107 74-75 0.5245109034665099 1107 76-77 0.4637125441210479 1107 78-79 0.4380734220737388 1107 80-81 0.4936693153134364 1107 82-83 0.44312972748492996 1107 84-85 0.4187008901026985 1107 86-87 0.5080464195932741 1107 88-89 0.4252983823447529 1107 90-91 0.38944881953017685 1107 92-93 0.32292503893165403 1107 94-95 0.4069868803627017 1107 96-97 0.2995187497768015 1107 98-99 0.13676026781563877 1107 100-101 0.09551234242022844 1108 1 0.03142088817104849 1108 2 0.144551670679121 1108 3 0.025343980391475895 1108 4 0.28341869205451076 1108 5 0.34202446132322706 1108 6 0.214175533628719 1108 7 0.0028871018328473497 1108 8 0.25088709401938303 1108 9 0.3193733423167018 1108 10-11 0.20895458033878356 1108 12-13 0.30542505554766564 1108 14-15 0.41742881106696217 1108 16-17 0.4309640137896693 1108 18-19 0.5531559483324955 1108 20-21 0.6031277941330231 1108 22-23 0.5243514809007692 1108 24-25 0.4603272731493391 1108 26-27 0.6728027209536833 1108 28-29 0.6217934933094824 1108 30-31 0.5586762865083088 1108 32-33 0.4875275063342954 1108 34-35 0.44108225605783247 1108 36-37 0.5408879957154298 1108 38-39 0.33599999205796394 1108 40-41 0.3142790775337687 1108 42-43 0.2894606747587858 1108 44-45 0.19722976409860848 1108 46-47 0.4132616518754375 1108 48-49 0.5060329993343515 1108 50-51 0.3361104988820003 1108 52-53 0.35557110721617846 1108 54-55 0.33370154941827934 1108 56-57 0.356057932954819 1108 58-59 0.3187446216635337 1108 60-61 0.2554030056340011 1108 62-63 0.3908468728418413 1108 64-65 0.45754134758879417 1108 66-67 0.5511333667802916 1108 68-69 0.52478764046311 1108 70-71 0.6374778013122508 1108 72-73 0.4855390750316033 1108 74-75 0.5611573767235747 1108 76-77 0.5997155631061837 1108 78-79 0.5346189752144461 1108 80-81 0.5237313845555427 1108 82-83 0.4320317785687564 1108 84-85 0.5600737432996397 1108 86-87 0.3483273439249466 1108 88-89 0.6266502058953982 1108 90-91 0.7481050852896125 1108 92-93 0.4925463730363724 1108 94-95 0.5356056306666908 1108 96-97 0.5422073318180232 1108 98-99 0.404384169508333 1108 100-101 0.46736013539532806 1109 1 0.2168663127303212 1109 2 0.19273127823526082 1109 3 0.17452904908169486 1109 4 0.263969511397633 1109 5 0.3235874027749901 1109 6 0.2778003840811891 1109 7 0.1859646364418026 1109 8 0.19951108373552984 1109 9 0.35687657548454865 1109 10-11 0.324271621101353 1109 12-13 0.29535606255725355 1109 14-15 0.2653876332682543 1109 16-17 0.21420865667925426 1109 18-19 0.13153410050895076 1109 20-21 0.35276425198246386 1109 22-23 0.2669224397437375 1109 24-25 0.2588090034802164 1109 26-27 0.3292554968066952 1109 28-29 0.30267117619000317 1109 30-31 0.3917424239302676 1109 32-33 0.2654999229791102 1109 34-35 0.21960636575801828 1109 36-37 0.27225629830015663 1109 38-39 0.24353333802119437 1109 40-41 0.20198834675565536 1109 42-43 0.3089976591712329 1109 44-45 0.6302950797120204 1109 46-47 0.4553427911962089 1109 48-49 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-2.0598034835633428 2316 68-69 -1.8604066144345381 2316 70-71 -1.9433302794958287 2316 72-73 -2.5525055662969294 2316 74-75 -2.615376423750906 2316 76-77 -2.817188801361741 2316 78-79 -3.0953604497861633 2316 80-81 -3.0625983932466916 2316 82-83 -2.575385004271755 2316 84-85 -2.5718542686601538 2316 86-87 -2.794732721010117 2316 88-89 -2.8117440269903575 2316 90-91 -2.7217157012684616 2316 92-93 -3.0835956464319203 2316 94-95 -2.7699996044936306 2316 96-97 -2.934076007018284 2316 98-99 -2.875423975501082 2316 100-101 -2.6556920419471055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5431.0 3 2047.0 4 2210.0 5 2314.0 6 2648.0 7 3095.0 8 3763.0 9 4655.0 10 5763.0 11 7067.0 12 8764.0 13 10210.0 14 11415.0 15 13025.0 16 15681.0 17 19740.0 18 17979.0 19 13228.0 20 13704.0 21 14735.0 22 16355.0 23 17988.0 24 20339.0 25 23791.0 26 27287.0 27 32109.0 28 37821.0 29 45925.0 30 55701.0 31 68881.0 32 86965.0 33 111195.0 34 150028.0 35 210380.0 36 310521.0 37 440135.0 38 374103.0 39 61038.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70765804502657 18.912923640052036 14.218914687658485 23.160503627262905 2 15.314506495528027 20.26046491938631 40.385070758284165 24.0399578268015 3 19.212411764083352 24.703410906858856 29.58469589696373 26.499481432094065 4 12.73503093762775 16.677566460445213 37.36827088405633 33.21913171787071 5 13.883021711204279 37.3333809765807 34.0473203320205 14.736276980194527 6 31.659397024776474 36.21918047486956 17.674382087790576 14.447040412563389 7 28.207221103635245 32.743804189365456 21.008827282821414 18.04014742417788 8 25.992707645944122 35.07286178580629 19.956842250131448 18.977588318118137 9 26.162950183156 17.06702548130821 20.430427622729514 36.33959671280628 10-11 24.640624982773502 26.808572565717014 27.561599192091137 20.989203259418346 12-13 25.662132337374565 24.183432599889912 27.993455640551563 22.160979422183956 14-15 22.670485890465507 25.522754603772803 26.284997118163243 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22.36180256460508 26.891846520508526 28.695319387399454 22.051031527486934 42-43 22.24874259694652 26.94445822269399 28.474505278171836 22.332293902187654 44-45 22.274275848112072 27.049994554834576 28.401784427003257 22.27394517005009 46-47 22.46073147229395 27.17220526090581 28.04783980385081 22.319223462949438 48-49 22.558916398137534 27.25011689477222 27.841347615401762 22.34961909168849 50-51 22.439210860532906 27.342243863768484 27.881756501300337 22.33678877439827 52-53 22.52985705385711 27.456342587509923 27.62238779713208 22.39141256150088 54-55 22.458330522520235 27.31032302525499 27.65421222939586 22.577134222828917 56-57 22.372842870118074 27.628391665123498 27.583353174078283 22.41541229068014 58-59 22.61206574420781 27.405449436901808 27.58690627228666 22.39557854660372 60-61 22.563386690558662 27.228410782439383 27.92345536829184 22.284747158710115 62-63 22.394822448812224 27.348610375031633 27.90570373655987 22.350863439596267 64-65 22.401373521051994 27.41286031394074 27.803241522028166 22.382524642979103 66-67 22.322639619157144 27.560573804177775 27.53259529449531 22.584191282169776 68-69 22.20013458368574 27.473724872493865 27.801497000971015 22.524643542849383 70-71 22.324740253840734 27.33932520245602 27.771338701582337 22.56459584212091 72-73 22.41195238273731 27.27578809039254 27.61922506190288 22.69303446496727 74-75 22.230338859947796 27.658223354484612 27.483327328540376 22.62811045702722 76-77 22.37811107869399 27.517790272702953 27.474589498926154 22.6295091496769 78-79 22.439213802966528 27.47972029452291 27.460382664575466 22.620683237935094 80-81 22.312843279728963 27.682315542870224 27.404592965052434 22.600248212348376 82-83 22.407398055216365 27.596229696444297 27.443731807487016 22.55264044085232 84-85 22.45013247439593 27.493001435874277 27.337374890192663 22.719491199537135 86-87 22.35620950698985 27.45793467320618 27.4934764706466 22.69237934915737 88-89 22.521291586309445 27.401770530420507 27.298980901016584 22.777956982253468 90-91 22.397089305402424 27.448423373759645 27.465556780595367 22.68893054024256 92-93 22.370426072900564 27.447686380706866 27.404882824990572 22.777004721401998 94-95 22.289248005982934 27.38746282714756 27.48980943511375 22.83347973175576 96-97 22.245124213166676 27.43063915958219 27.433615830420603 22.89062079683053 98-99 22.2186670735854 27.44996906212141 27.315412444039488 23.015951420253707 100-101 22.409242008592653 27.373692174316915 27.262430091795075 22.954635725295354 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 352.0 1 259.5 2 221.5 3 297.0 4 273.0 5 296.0 6 516.5 7 988.5 8 1672.0 9 2095.5 10 1983.5 11 1865.5 12 2054.5 13 2193.5 14 2318.5 15 2434.0 16 2515.0 17 2688.0 18 2856.5 19 3236.5 20 3657.0 21 4211.5 22 5186.5 23 6478.0 24 8180.5 25 10450.0 26 13365.0 27 16817.5 28 19816.0 29 22707.0 30 26659.0 31 30758.0 32 34434.5 33 38528.0 34 44163.0 35 48827.5 36 53554.0 37 58485.0 38 61651.0 39 64415.0 40 67362.5 41 70996.0 42 74296.5 43 78483.0 44 82523.0 45 85198.0 46 86871.0 47 88603.0 48 92443.5 49 91082.5 50 84719.0 51 80042.0 52 77134.0 53 75603.5 54 72871.0 55 67575.0 56 61659.5 57 56022.0 58 51224.5 59 46197.0 60 39605.0 61 32174.5 62 25234.0 63 19746.0 64 15089.0 65 11543.5 66 8748.0 67 6675.0 68 4841.5 69 3211.0 70 2208.0 71 1519.5 72 1046.5 73 733.5 74 502.0 75 345.5 76 236.5 77 133.0 78 77.0 79 48.5 80 37.5 81 30.0 82 17.5 83 13.0 84 9.5 85 6.5 86 8.5 87 8.5 88 8.0 89 8.5 90 4.5 91 2.5 92 2.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022926981971455908 2 0.012301207634684997 3 0.01260984008430075 4 0.01953202502568263 5 0.053878407632921385 6 0.038755417601749505 7 0.014461634781995263 8 0.0457657832430216 9 0.026630571366844935 10-11 0.021494045598239912 12-13 0.03412593085751321 14-15 0.018473856627000046 16-17 0.01602684220504658 18-19 0.015872525980238708 20-21 0.013271195333477363 22-23 0.007517404665640831 24-25 0.001829749522721962 26-27 1.1022587486276879E-4 28-29 0.002094291622392607 30-31 0.0 32-33 2.645420996706451E-4 34-35 2.2045174972553756E-5 36-37 0.0 38-39 1.543162248078763E-4 40-41 6.613552491766127E-5 42-43 1.1022587486276879E-4 44-45 1.3227104983532255E-4 46-47 2.2045174972553756E-5 48-49 1.9840657475298382E-4 50-51 1.9840657475298382E-4 52-53 5.731745492863977E-4 54-55 0.001895885047639623 56-57 4.4090349945107515E-4 58-59 0.001653388122941532 60-61 0.003791770095279246 62-63 0.002116336797365161 64-65 0.0013447556733257792 66-67 0.011375310285837738 68-69 0.014814357581556125 70-71 0.02927599236355139 72-73 0.017856591727768545 74-75 0.019267482926011985 76-77 0.033045717283858085 78-79 0.020744509649173085 80-81 0.046713725766841414 82-83 0.03639658387968625 84-85 0.02036974167463967 86-87 0.013998686107571635 88-89 0.014615951006803142 90-91 0.025131499468711283 92-93 0.03240640720965402 94-95 0.01260984008430075 96-97 0.019223392576066877 98-99 0.02746828801580198 100-101 0.03970336012556932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2268070.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.55403213155852 #Duplication Level Percentage of deduplicated Percentage of total 1 77.68386984892726 46.26387681086824 2 12.935619948940214 15.407366521616298 3 4.116277089896184 7.354226942222267 4 1.7458279782033208 4.158843820403776 5 0.9440084665247632 2.810975527393952 6 0.5570475070188452 1.9904655079082927 7 0.379562763912012 1.5823145126571385 8 0.27139003093366637 1.292989649792658 9 0.19701681006986277 1.0559830893621993 >10 1.0733497085819368 11.463351520817715 >50 0.0684093186840318 2.7811790429480143 >100 0.02643107199437027 2.652617021411912 >500 8.177512154939426E-4 0.33607691000038104 >1k 2.2302305877099185E-4 0.2787432782718734 >5k 1.4868203918066124E-4 0.5709898443253416 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7399 0.3262244992438505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5498 0.24240874399820111 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3351 0.14774676266605527 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.409034994510751E-5 0.0 5 0.0 0.0 0.0 4.409034994510751E-5 0.0 6 0.0 0.0 0.0 4.409034994510751E-5 0.0 7 0.0 0.0 0.0 4.409034994510751E-5 0.0 8 0.0 0.0 0.0 4.409034994510751E-5 0.0 9 0.0 4.409034994510751E-5 0.0 4.409034994510751E-5 0.0 10-11 0.0 4.409034994510751E-5 0.0 4.409034994510751E-5 0.0 12-13 0.0 4.409034994510751E-5 0.0 4.409034994510751E-5 0.0 14-15 0.0 4.409034994510751E-5 0.0 4.409034994510751E-5 0.0 16-17 0.0 4.409034994510751E-5 0.0 4.409034994510751E-5 0.0 18-19 0.0 4.409034994510751E-5 0.0 6.613552491766127E-5 0.0 20-21 0.0 4.409034994510751E-5 0.0 1.1022587486276879E-4 0.0 22-23 0.0 4.409034994510751E-5 0.0 2.8658727464319885E-4 0.0 24-25 0.0 4.409034994510751E-5 0.0 4.4090349945107515E-4 0.0 26-27 0.0 4.409034994510751E-5 0.0 7.715811240393815E-4 0.0 28-29 0.0 4.409034994510751E-5 0.0 0.0014770267231611017 0.0 30-31 0.0 4.409034994510751E-5 0.0 0.00328473107091051 0.0 32-33 0.0 4.409034994510751E-5 0.0 0.0056435647929737615 0.0 34-35 0.0 4.409034994510751E-5 0.0 0.007826037115256583 0.0 36-37 0.0 4.409034994510751E-5 0.0 0.01062577433677091 0.0 38-39 0.0 4.409034994510751E-5 0.0 0.013050743583751825 0.0 40-41 0.0 4.409034994510751E-5 0.0 0.016092977729964243 0.0 42-43 0.0 4.409034994510751E-5 0.0 0.021207458323596714 0.0 44-45 0.0 4.409034994510751E-5 0.0 0.02682897794159792 0.0 46-47 0.0 4.409034994510751E-5 0.0 0.0325607234344619 0.0 48-49 0.0 4.409034994510751E-5 0.0 0.03838064962721609 0.0 50-51 0.0 4.409034994510751E-5 0.0 0.045920099467829475 0.0 52-53 0.0 4.409034994510751E-5 0.0 0.0665984735920849 0.0 54-55 0.0 4.409034994510751E-5 0.0 0.09221496691019236 0.0 56-57 0.0 4.409034994510751E-5 0.0 0.11489945195695017 0.0 58-59 0.0 4.409034994510751E-5 0.0 0.15175898451106007 0.0 60-61 0.0 4.409034994510751E-5 0.0 0.1973043160043561 0.0 62-63 0.0 4.409034994510751E-5 0.0 0.23063662056285741 0.0 64-65 0.0 4.409034994510751E-5 0.0 0.25521699065725484 0.0 66-67 0.0 4.409034994510751E-5 0.0 0.3229618133479125 0.0 68-69 0.0 4.409034994510751E-5 0.0 0.39709973678061083 0.0 70-71 0.0 4.409034994510751E-5 0.0 0.42758821376765266 0.0 72-73 0.0 4.409034994510751E-5 0.0 0.4892485681658856 0.0 74-75 0.0 4.409034994510751E-5 0.0 0.5815517157759681 4.409034994510751E-5 76-77 0.0 4.409034994510751E-5 0.0 0.6723998818378621 8.818069989021502E-5 78-79 0.0 4.409034994510751E-5 0.0 0.7550692879849388 8.818069989021502E-5 80-81 0.0 4.409034994510751E-5 0.0 0.8639063168244366 8.818069989021502E-5 82-83 0.0 4.409034994510751E-5 0.0 0.993664216712888 8.818069989021502E-5 84-85 0.0 4.409034994510751E-5 0.0 1.1378837513833346 8.818069989021502E-5 86-87 0.0 4.409034994510751E-5 0.0 1.348569488596031 8.818069989021502E-5 88-89 0.0 4.409034994510751E-5 0.0 1.602155136305317 8.818069989021502E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6825 0.0 33.630474 1 GTATCAA 10270 0.0 33.269554 1 ATCAACG 12220 0.0 27.767366 3 TATCAAC 12290 0.0 27.647882 2 TCAACGC 12290 0.0 27.609215 4 CAACGCA 12495 0.0 27.080172 5 AACGCAG 12765 0.0 26.54227 6 TACATGG 8825 0.0 23.909801 2 GTACATG 9045 0.0 23.905144 1 ACGCAGA 14330 0.0 23.60935 7 GAGTACT 6790 0.0 23.195648 12-13 CGCAGAG 14425 0.0 23.19085 8 ACATGGG 8910 0.0 22.188265 3 GCAGAGT 15160 0.0 21.531734 9 GTACTTT 7370 0.0 20.91434 14-15 AGAGTAC 12310 0.0 19.375921 10-11 AGTACTT 7440 0.0 19.189528 12-13 CATGGGG 4255 0.0 18.3169 4 CAGAGTA 14740 0.0 18.051247 10-11 ACTTTTT 9125 0.0 17.411886 16-17 >>END_MODULE