FastQCFastQC Report
Thu 26 May 2016
SRR936857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268070
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87210.3845119418712826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69370.3058547575692108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.2070041929922798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72150.034.306461
GTATCAA107450.031.657831
ATCAACG131750.025.6672573
TCAACGC132200.025.543964
TATCAAC132050.025.503292
CAACGCA135300.024.9268885
AACGCAG138850.024.3586
CGCACGA550.002877020721.59302928-29
GAGTACT82600.021.56260712-13
ACGCAGA157250.021.2057677
CGCAGAG157700.021.1151338
TACATGG84500.021.0796282
GTACATG85250.021.065511
ACATGGG84050.020.0604523
GTACTTT87700.019.98374214-15
GCAGAGT165200.019.8114689
AGAGTAC132450.019.42021410-11
AGTACTT88350.019.2722612-13
CGCGGAA3150.018.84814882-83
CCCGCGA3200.018.55855620-21
Produced by FastQC (version 0.11.5)