##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268070 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17044976565979 33.0 31.0 34.0 31.0 34.0 2 32.29125467908839 33.0 31.0 34.0 31.0 34.0 3 32.40813511046837 34.0 31.0 34.0 31.0 34.0 4 36.05731172318315 37.0 35.0 37.0 35.0 37.0 5 35.96319646219032 37.0 35.0 37.0 35.0 37.0 6 36.001268038464424 37.0 35.0 37.0 35.0 37.0 7 35.94974008738707 37.0 35.0 37.0 35.0 37.0 8 35.91257765412884 37.0 35.0 37.0 35.0 37.0 9 37.601724814489856 39.0 37.0 39.0 35.0 39.0 10-11 37.50591251592763 39.0 37.0 39.0 35.0 39.0 12-13 37.40729276433267 39.0 37.0 39.0 34.0 39.0 14-15 38.61912000070545 40.0 38.0 41.0 34.0 41.0 16-17 38.522967765545154 40.0 38.0 41.0 34.0 41.0 18-19 38.68360853060091 40.0 38.0 41.0 34.0 41.0 20-21 38.711130608843646 40.0 38.0 41.0 34.0 41.0 22-23 38.63402716847364 40.0 38.0 41.0 34.0 41.0 24-25 38.530011860304135 40.0 38.0 41.0 34.0 41.0 26-27 38.35502916576649 40.0 38.0 41.0 34.0 41.0 28-29 38.27561428880061 40.0 38.0 41.0 34.0 41.0 30-31 38.27744139290233 40.0 38.0 41.0 34.0 41.0 32-33 38.126509763807995 40.0 38.0 41.0 33.5 41.0 34-35 37.98522929186489 40.0 37.0 41.0 33.0 41.0 36-37 37.83077506426169 40.0 37.0 41.0 33.0 41.0 38-39 37.67595797307843 40.0 37.0 41.0 33.0 41.0 40-41 37.501264290784675 40.0 37.0 41.0 33.0 41.0 42-43 37.25591868857663 40.0 36.5 41.0 32.0 41.0 44-45 37.01023822016075 40.0 36.0 41.0 31.0 41.0 46-47 36.68638137270896 39.0 36.0 41.0 30.5 41.0 48-49 36.34870793229486 39.0 36.0 41.0 30.0 41.0 50-51 36.064607132936814 39.0 35.0 40.0 29.5 41.0 52-53 35.82003156869057 39.0 35.0 40.0 29.0 41.0 54-55 35.53828475311609 38.0 35.0 40.0 28.0 41.0 56-57 35.25045589421843 38.0 34.5 40.0 27.5 41.0 58-59 34.88496607247572 38.0 34.0 40.0 26.5 41.0 60-61 34.549159637930046 37.5 34.0 40.0 26.0 41.0 62-63 34.169890700022485 37.0 33.0 40.0 26.0 41.0 64-65 33.77489936377625 36.5 33.0 39.0 25.5 40.5 66-67 33.37323164628958 36.0 32.0 39.0 25.0 40.0 68-69 33.05747221205695 35.5 32.0 39.0 23.5 40.0 70-71 32.582576375508694 35.0 32.0 38.0 22.5 40.0 72-73 32.071814141538844 35.0 31.0 37.5 21.5 39.5 74-75 31.535176383444956 35.0 31.0 37.0 20.0 39.0 76-77 30.143404745003462 33.5 29.5 35.0 19.5 37.5 78-79 30.892901233207088 34.0 30.5 36.0 20.0 38.0 80-81 30.674068701583288 34.0 30.0 35.5 18.0 37.0 82-83 30.32912432155974 34.0 30.0 35.0 17.5 37.0 84-85 29.95309889024589 34.0 30.0 35.0 13.5 36.0 86-87 29.577335796514216 34.0 29.5 35.0 8.5 36.0 88-89 29.16454430418814 34.0 29.0 35.0 4.5 35.5 90-91 28.674157984541925 33.5 29.0 35.0 2.0 35.0 92-93 28.162084503564706 33.0 28.5 35.0 2.0 35.0 94-95 27.540250080464887 33.0 27.0 35.0 2.0 35.0 96-97 26.843192890871975 32.5 25.5 34.0 2.0 35.0 98-99 25.93367819335382 32.0 24.5 34.0 2.0 35.0 100-101 22.226799216955385 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2398324097664286 1101 2 0.19087783005920755 1101 3 0.2816158901308867 1101 4 0.1871138542201436 1101 5 0.18657231149647657 1101 6 0.2375328777263661 1101 7 0.30578120689082766 1101 8 0.23595639684426573 1101 9 0.36943974648182376 1101 10-11 0.4151065709264472 1101 12-13 0.3674743062797461 1101 14-15 0.4159266155264234 1101 16-17 0.40139615175500154 1101 18-19 0.3908250692533599 1101 20-21 0.44577646147956074 1101 22-23 0.38978756960158734 1101 24-25 0.41387419502714806 1101 26-27 0.2767466943373904 1101 28-29 0.4043355527224648 1101 30-31 0.47837032523257506 1101 32-33 0.41372577434090374 1101 34-35 0.42958202905384013 1101 36-37 0.4785804390287609 1101 38-39 0.5141449856314537 1101 40-41 0.503680933783933 1101 42-43 0.5570429847174978 1101 44-45 0.5021928857686149 1101 46-47 0.5682425109336791 1101 48-49 0.546790325968388 1101 50-51 0.4893077172039071 1101 52-53 0.5591116473372395 1101 54-55 0.564002390178814 1101 56-57 0.525929165491668 1101 58-59 0.4057586814418812 1101 60-61 0.5887798092190266 1101 62-63 0.5884608397903293 1101 64-65 0.5432116656626818 1101 66-67 0.26923682566497575 1101 68-69 0.28269252284957247 1101 70-71 0.25479644673347224 1101 72-73 0.5308880494834938 1101 74-75 0.4273129938083109 1101 76-77 0.21221567168796795 1101 78-79 0.26522881437867696 1101 80-81 0.5359896619781601 1101 82-83 0.5503926754664228 1101 84-85 0.4660050320668816 1101 86-87 0.23583261664275668 1101 88-89 0.19399659696902916 1101 90-91 0.5755644783076477 1101 92-93 0.5188546449059253 1101 94-95 0.2625939353267732 1101 96-97 0.2521462084555246 1101 98-99 0.359264094971973 1101 100-101 0.8596766529145121 1102 1 0.30260863539720617 1102 2 0.3235269852184004 1102 3 0.28284054439271245 1102 4 0.19131246324973006 1102 5 0.2134032796044778 1102 6 0.19909657845597906 1102 7 0.20204762521397157 1102 8 0.26324087969150156 1102 9 0.1662579479237749 1102 10-11 0.33231104110980425 1102 12-13 0.28824008010212054 1102 14-15 0.3905513762799231 1102 16-17 0.3805055894255034 1102 18-19 0.3407462284090741 1102 20-21 0.3685247690225779 1102 22-23 0.37846542817325 1102 24-25 0.37802874775183426 1102 26-27 0.3865671592413946 1102 28-29 0.5060108435701451 1102 30-31 0.5104658470315044 1102 32-33 0.5117093195268865 1102 34-35 0.44723775257020293 1102 36-37 0.4020880651073 1102 38-39 0.4500677318831734 1102 40-41 0.5182050208439577 1102 42-43 0.49687508558444193 1102 44-45 0.5516398691555082 1102 46-47 0.509639710490454 1102 48-49 0.4936972362090657 1102 50-51 0.2938793554693646 1102 52-53 0.34909423450930177 1102 54-55 0.34569856819671685 1102 56-57 0.39296166601076976 1102 58-59 0.5204205086992246 1102 60-61 0.46913373985141504 1102 62-63 0.4786174524505 1102 64-65 0.4343034725879278 1102 66-67 0.15775895558071085 1102 68-69 0.35996343725945934 1102 70-71 0.4064459369919575 1102 72-73 0.3722279943462965 1102 74-75 0.40236875802998995 1102 76-77 0.37339082171171256 1102 78-79 0.4022820581603206 1102 80-81 0.5156921020354766 1102 82-83 0.4587458988954154 1102 84-85 0.4337884222437438 1102 86-87 0.28315336422253523 1102 88-89 0.4182113236575269 1102 90-91 0.5548352981496407 1102 92-93 0.6795069989270139 1102 94-95 0.44053360923199847 1102 96-97 0.6442717863990026 1102 98-99 0.8532911043855904 1102 100-101 0.9526909635042706 1103 1 0.273120591831038 1103 2 0.28777780223389016 1103 3 0.28226601251038375 1103 4 0.15507262165038327 1103 5 0.20658618024161512 1103 6 0.18287968218624684 1103 7 0.19508794129637153 1103 8 0.15777563767218794 1103 9 0.24968249343550752 1103 10-11 0.324692352947622 1103 12-13 0.3134212313825344 1103 14-15 0.3718518312923962 1103 16-17 0.3561032613432431 1103 18-19 0.4031763964252022 1103 20-21 0.3630370609494804 1103 22-23 0.3861530122337271 1103 24-25 0.33177323982431517 1103 26-27 0.38506103499513245 1103 28-29 0.34550197247437353 1103 30-31 0.43693894617869233 1103 32-33 0.4284806780042203 1103 34-35 0.5257922567144391 1103 36-37 0.3534238966658094 1103 38-39 0.31990121349246436 1103 40-41 0.4646387594649113 1103 42-43 0.5460628628529918 1103 44-45 0.5079221266644112 1103 46-47 0.4311867529093476 1103 48-49 0.5534248877276227 1103 50-51 0.46234600236590495 1103 52-53 0.5017561623363633 1103 54-55 0.5377563688599096 1103 56-57 0.4592479074998792 1103 58-59 0.43662332624191436 1103 60-61 0.4623037599510482 1103 62-63 0.6158952257350663 1103 64-65 0.5664467041650099 1103 66-67 0.57155550898851 1103 68-69 0.3631179707593404 1103 70-71 0.35556122379360033 1103 72-73 0.4113162320191748 1103 74-75 0.58985486652006 1103 76-77 0.4938412211581529 1103 78-79 0.5551204193964914 1103 80-81 0.6769618234863621 1103 82-83 0.7094742483593741 1103 84-85 0.6101906784346411 1103 86-87 0.6518404779936304 1103 88-89 0.7604537952829027 1103 90-91 0.6592323541979503 1103 92-93 0.7839367056401692 1103 94-95 0.6319449866407361 1103 96-97 0.495916151762934 1103 98-99 0.6144727589056096 1103 100-101 0.9126154176540489 1104 1 0.18708606273177963 1104 2 0.21680672135528312 1104 3 0.13623159778376248 1104 4 0.07896449375890313 1104 5 0.13640884546718723 1104 6 0.12377190313130626 1104 7 0.12317728920078252 1104 8 0.10354184856389992 1104 9 0.20262158428741373 1104 10-11 0.2108515482217399 1104 12-13 0.20556640457130015 1104 14-15 0.17655426228280646 1104 16-17 0.26335448656011806 1104 18-19 0.24973406596834025 1104 20-21 0.23727348062843845 1104 22-23 0.22653799053151857 1104 24-25 0.13555296956176477 1104 26-27 0.06331422170274692 1104 28-29 0.03954001281359609 1104 30-31 0.20168415253552752 1104 32-33 0.15849348773961935 1104 34-35 0.20975063651165726 1104 36-37 0.32370216529282203 1104 38-39 0.3534072495084075 1104 40-41 0.3462507846040168 1104 42-43 0.24803979389200492 1104 44-45 0.22310966919673092 1104 46-47 0.18178978264052148 1104 48-49 -0.010435932209823306 1104 50-51 0.10062023363881423 1104 52-53 0.06417359958867053 1104 54-55 0.13799912478298637 1104 56-57 0.21515296677349482 1104 58-59 0.13984949241392286 1104 60-61 0.36788657431853977 1104 62-63 0.4548182357941357 1104 64-65 0.3340457937588184 1104 66-67 0.34157672511276616 1104 68-69 0.43279378151837733 1104 70-71 0.29126350038094273 1104 72-73 0.37436157505131007 1104 74-75 0.3990498547110839 1104 76-77 0.3591051074260001 1104 78-79 0.3178046308257514 1104 80-81 0.6503344438206717 1104 82-83 0.45815839116504975 1104 84-85 0.4287379171932386 1104 86-87 0.23622489586549733 1104 88-89 0.26069740900075544 1104 90-91 0.40526819858254015 1104 92-93 0.3605006084920497 1104 94-95 0.3382969569953467 1104 96-97 0.4007446785861788 1104 98-99 0.44464340288074666 1104 100-101 0.8871477765325082 1105 1 0.24812738934687673 1105 2 0.2718950756239593 1105 3 0.2128561571952119 1105 4 0.15413540663473668 1105 5 0.13949710511282376 1105 6 0.16534690372269978 1105 7 0.20044173695703194 1105 8 0.18705969683584556 1105 9 0.24550593127012377 1105 10-11 0.17779262172019372 1105 12-13 0.20166616091695033 1105 14-15 0.31823807937646365 1105 16-17 0.23330102453944335 1105 18-19 0.14413179028409928 1105 20-21 0.3151299138127328 1105 22-23 0.32596552110659616 1105 24-25 0.12248434419806387 1105 26-27 0.11009881233979257 1105 28-29 0.13700411519572953 1105 30-31 0.12258993163802501 1105 32-33 0.2274949780525901 1105 34-35 0.276944826656667 1105 36-37 0.22099535479912902 1105 38-39 0.24938931844129542 1105 40-41 0.15452616132201058 1105 42-43 0.35043907948801234 1105 44-45 0.2967311297034385 1105 46-47 0.21319162305875494 1105 48-49 0.06741754400431432 1105 50-51 0.25037539650872986 1105 52-53 0.2690916323755559 1105 54-55 0.377026399875831 1105 56-57 0.347453134729399 1105 58-59 0.3639978850049346 1105 60-61 0.3024884478548415 1105 62-63 0.2286945871295103 1105 64-65 0.1133730239057229 1105 66-67 0.17812251083888242 1105 68-69 0.24891459149164774 1105 70-71 -0.04626399944163495 1105 72-73 0.1160637253600143 1105 74-75 0.2558556172545501 1105 76-77 0.05825941395735512 1105 78-79 0.4177898459748981 1105 80-81 0.325806967653854 1105 82-83 0.42980459559813866 1105 84-85 0.3681561037425709 1105 86-87 0.45141662581132636 1105 88-89 0.15366632533033098 1105 90-91 0.2576781292711594 1105 92-93 0.393346633141352 1105 94-95 0.3755583295026206 1105 96-97 0.26164939317941815 1105 98-99 0.29655715397482396 1105 100-101 0.685480636750615 1106 1 0.14924080939356799 1106 2 0.1586456336167288 1106 3 0.19580723341573503 1106 4 0.18223258716783874 1106 5 0.20363035635067206 1106 6 0.20929107879132403 1106 7 0.17729594050253183 1106 8 0.2135778219801523 1106 9 0.2346013416192818 1106 10-11 0.15155028610357846 1106 12-13 0.27612612653947366 1106 14-15 0.30706154842389566 1106 16-17 0.2939593277283379 1106 18-19 0.032744248274298116 1106 20-21 0.29866912723534966 1106 22-23 0.251118808774315 1106 24-25 0.3331323854510444 1106 26-27 0.31064975002418294 1106 28-29 0.3049201725599531 1106 30-31 0.2854442891125615 1106 32-33 0.3515579350294189 1106 34-35 0.29988538439288703 1106 36-37 0.2801804288133454 1106 38-39 0.21317494660004144 1106 40-41 0.21985300398088015 1106 42-43 0.18500705198602674 1106 44-45 -0.006339754618892357 1106 46-47 0.09704190141376756 1106 48-49 0.1978044189882766 1106 50-51 0.05861060007988783 1106 52-53 0.18483087658398034 1106 54-55 -0.06268985759613344 1106 56-57 0.04039228144942797 1106 58-59 0.18605399213954854 1106 60-61 -0.018632540369594608 1106 62-63 0.17928015884761095 1106 64-65 -0.056006642068268775 1106 66-67 0.13709529105386054 1106 68-69 0.05079573261891568 1106 70-71 0.04208357232777615 1106 72-73 0.1401593922575728 1106 74-75 0.19179419956810406 1106 76-77 0.1566906205044738 1106 78-79 0.16846007866429602 1106 80-81 0.19915158911669195 1106 82-83 0.16674628214730447 1106 84-85 0.15879401133383908 1106 86-87 0.033020822943115036 1106 88-89 0.11695617371022493 1106 90-91 0.22847714925627827 1106 92-93 0.35365346502518946 1106 94-95 0.21453316210478945 1106 96-97 0.32299332196158304 1106 98-99 0.3858116243306604 1106 100-101 0.2365959809102911 1107 1 0.21698620337047458 1107 2 0.13356410684850317 1107 3 0.13585224597713363 1107 4 0.1838252575231536 1107 5 0.07186352822590436 1107 6 0.14000321750489064 1107 7 0.0872640105145095 1107 8 0.17380764982199537 1107 9 0.14425436624642884 1107 10-11 0.1971927255461594 1107 12-13 0.1580096342007593 1107 14-15 0.22764043634133913 1107 16-17 0.09920480147703614 1107 18-19 0.16423868233068362 1107 20-21 0.12128166395696383 1107 22-23 0.19403103215234552 1107 24-25 0.2530401267181617 1107 26-27 0.26784133633406526 1107 28-29 0.25517650590817453 1107 30-31 0.2328920173183704 1107 32-33 0.27731720291585304 1107 34-35 0.224357348331786 1107 36-37 0.4095571877812887 1107 38-39 0.3928447339390928 1107 40-41 0.40783788350420735 1107 42-43 0.45313279785224836 1107 44-45 0.2599950614060944 1107 46-47 0.1474084261930102 1107 48-49 0.22489194034052673 1107 50-51 0.21808865858345428 1107 52-53 0.16336556454864848 1107 54-55 0.4003684651541377 1107 56-57 0.43222428844381255 1107 58-59 0.33447217074751734 1107 60-61 0.2946337208436631 1107 62-63 0.2852006082925058 1107 64-65 0.35433199449359165 1107 66-67 0.38808988571745573 1107 68-69 0.4411055875193526 1107 70-71 0.35724904029036253 1107 72-73 0.3053988884402301 1107 74-75 0.3251390200390354 1107 76-77 0.3554457551486152 1107 78-79 0.4069585821455384 1107 80-81 0.5333870882009144 1107 82-83 0.5703660068389063 1107 84-85 0.6760356730546562 1107 86-87 0.3842955761291975 1107 88-89 0.34107703541572576 1107 90-91 0.3858081766531001 1107 92-93 0.4768345086954575 1107 94-95 0.49056992945409306 1107 96-97 0.6490030732650531 1107 98-99 0.5677690538496236 1107 100-101 0.5367964740063087 1108 1 0.1567671349552242 1108 2 0.08461311458540166 1108 3 0.07042461757000495 1108 4 0.09584761064201786 1108 5 0.04029516630266272 1108 6 0.05501533013744364 1108 7 0.0854097836366634 1108 8 0.07555495786514399 1108 9 0.13718880614277396 1108 10-11 0.1452102685974097 1108 12-13 0.18087334809857936 1108 14-15 0.16541013334206411 1108 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-1.5174742040188818 2316 86-87 -1.5114482643076634 2316 88-89 -1.6684286732503253 2316 90-91 -1.5706922992529577 2316 92-93 -1.584034023542582 2316 94-95 -1.4730396230912355 2316 96-97 -1.3821151698986682 2316 98-99 -1.4920043243919814 2316 100-101 -1.4864723533376214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 17.0 5 42.0 6 93.0 7 212.0 8 517.0 9 1138.0 10 1839.0 11 3145.0 12 4604.0 13 6646.0 14 8539.0 15 11447.0 16 14923.0 17 20127.0 18 18538.0 19 12412.0 20 11363.0 21 11838.0 22 12590.0 23 13926.0 24 16458.0 25 19708.0 26 23851.0 27 29821.0 28 37276.0 29 46756.0 30 58291.0 31 73070.0 32 93324.0 33 121439.0 34 164661.0 35 231829.0 36 351883.0 37 502415.0 38 324180.0 39 19146.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.67493844458293 19.584523537036226 14.93974118279015 22.800796835590695 2 15.611631649727729 20.74679776891025 39.75607927864371 23.885491302718314 3 19.500314364749528 24.67009337470779 29.905699393227536 25.92389286731515 4 13.206467368851746 17.113237154347125 37.06325617939184 32.61703929740929 5 13.890091575656834 37.303169655257555 34.19413862887829 14.612600140207313 6 31.803251222404956 36.4628075853038 17.32852160647599 14.405419585815253 7 28.120781104639626 32.768036259903795 21.011079904941205 18.10010273051537 8 26.142403012252707 35.136613949304916 19.766365235640876 18.9546178028015 9 26.08773097832077 16.82170303385698 20.422165100724403 36.66840088709784 10-11 24.73172332049274 26.908142135489427 27.434884258279364 20.925250285738475 12-13 25.81104198724025 24.154721856027372 28.081055699339085 21.95318045739329 14-15 22.652872265847172 25.499345258303315 26.406371937374068 25.441410538475445 16-17 21.95121843682073 28.733923556151264 26.98951090574806 22.325347101279945 18-19 21.900447760437252 27.248485548587553 29.41462669398216 21.43643999699303 20-21 22.719091869469903 26.334630192359583 29.51225813961281 21.4340197985577 22-23 22.347742562859192 26.363261788723275 29.407980642192953 21.881015006224576 24-25 22.200292218765895 26.4907533507377 29.56610562162851 21.742848808867894 26-27 22.14766891010039 26.649197161139217 29.406530649678654 21.796603279081744 28-29 21.947344925459504 26.730370084598636 29.599735457025545 21.72254953291631 30-31 22.13528077472612 26.471719705652752 29.55145752769564 21.84154199192549 32-33 21.990267867339284 26.59559223281279 29.48871029400547 21.925429605842453 34-35 22.364874100005732 26.556852301736715 29.328944873835468 21.749328724422085 36-37 22.455360656615973 26.432351406308253 29.255815091734732 21.856472845341038 38-39 22.1445169022851 26.61223840048012 29.364497186755806 21.878747510478973 40-41 22.39228916215118 26.557117778722798 29.262110242441842 21.788482816684184 42-43 22.183971394180958 26.66857724849762 29.014382272152094 22.13306908516933 44-45 22.31478746246809 26.75067348009541 28.865489160387465 22.069049897049034 46-47 22.497420703156003 26.88910346287135 28.495806989233092 22.11766884473956 48-49 22.550362025455428 27.05734360639214 28.238646898031682 22.153647470120745 50-51 22.542768560012895 27.166030220903743 28.126486072012252 22.164715147071114 52-53 22.56735903212864 27.27303390107007 27.95107734770091 22.20852971910038 54-55 22.539216777759282 27.06992166013926 27.98716050798004 22.403701054121413 56-57 22.50636409151064 27.39446256247524 27.864524601757896 22.234648744256226 58-59 22.697994679167184 27.180844825503314 27.894602714383094 22.22655778094641 60-61 22.594381623424105 26.988066124950645 28.317408052196626 22.100144199428627 62-63 22.449063277543132 27.02559700100663 28.31316323340657 22.212176488043667 64-65 22.498477779996993 27.12862792093186 28.11790558400119 22.254988715069956 66-67 22.351859906645878 27.268989548420976 27.942172735331383 22.436977809601764 68-69 22.270057992267404 27.193889405972765 28.14952948796351 22.386523113796322 70-71 22.409019902991602 27.02509776014737 28.125535977806493 22.440346359054537 72-73 22.483433051008124 27.007940672025114 27.960446547064244 22.548179729902515 74-75 22.41604095111703 27.376712358965992 27.707389983554297 22.499856706362678 76-77 22.42657854475391 27.27843496893835 27.80606418673145 22.48892229957629 78-79 22.521224819398068 27.247022264373143 27.66731899187867 22.564433924350116 80-81 22.49761192325439 27.356215513978267 27.64397517943368 22.502197383333662 82-83 22.51027132616132 27.30026445840044 27.7157033612343 22.47376085420394 84-85 22.47977383647061 27.273865682444658 27.60044296163461 22.64591751945012 86-87 22.434891381101004 27.154603497312717 27.691376339093697 22.719128782492582 88-89 22.607536073158414 27.190474521337464 27.523644058623553 22.67834534688057 90-91 22.45928238230029 27.18484078904438 27.732842164669364 22.623034663985962 92-93 22.317016579092115 27.105211815785907 27.696375879465666 22.881395725656315 94-95 22.3151891950401 27.092093077394676 27.698754381203926 22.8939633463613 96-97 22.269223426352507 27.10007420546581 27.677382147567027 22.953320220614657 98-99 22.252960924004054 27.117002242462885 27.438292588937944 23.191744244595117 100-101 22.04794259780302 27.209211017401824 27.490551974556304 23.252294410238854 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 512.0 1 384.5 2 300.0 3 360.5 4 317.0 5 299.5 6 546.5 7 1146.0 8 1911.5 9 2309.0 10 2205.0 11 2075.5 12 2156.0 13 2384.0 14 2609.5 15 2790.5 16 3005.0 17 3188.0 18 3382.0 19 3624.0 20 4021.5 21 4655.0 22 5556.0 23 6848.5 24 8600.5 25 10716.5 26 13420.5 27 16894.5 28 19928.5 29 22957.0 30 26959.5 31 30708.0 32 34154.0 33 38300.0 34 43763.0 35 48476.5 36 53440.5 37 58321.0 38 61200.5 39 64077.5 40 67231.0 41 70815.5 42 74453.5 43 78523.0 44 82589.0 45 85506.0 46 86993.5 47 88391.5 48 92383.0 49 90742.5 50 83926.5 51 79869.5 52 77235.0 53 75172.0 54 72208.0 55 67612.0 56 61916.0 57 55934.0 58 50952.5 59 46208.0 60 39545.0 61 31833.5 62 25027.5 63 19515.0 64 14851.5 65 11181.5 66 8473.0 67 6540.0 68 4636.0 69 3036.5 70 2051.5 71 1366.0 72 945.0 73 681.0 74 511.0 75 371.0 76 231.0 77 127.5 78 78.5 79 50.5 80 31.5 81 15.5 82 11.0 83 13.0 84 12.0 85 7.0 86 4.0 87 2.5 88 2.5 89 2.0 90 1.5 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02649830031700962 2 0.0052908419934129015 3 1.7636139978043005E-4 4 3.086324496157526E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011551671685618168 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.9840657475298382E-4 20-21 0.0011463490985727954 22-23 0.0019620205725572842 24-25 0.010956451961359217 26-27 0.005202661293522686 28-29 3.3067762458830635E-4 30-31 0.003020188971239865 32-33 0.005246751643467795 34-35 0.0 36-37 0.00879602481404895 38-39 0.013866415057736314 40-41 0.001829749522721962 42-43 0.0 44-45 0.0 46-47 4.4090349945107515E-4 48-49 0.0029320082713496496 50-51 0.006018332767507175 52-53 0.0 54-55 0.0029760986212947573 56-57 0.0031524600210751873 58-59 1.7636139978043005E-4 60-61 0.0013447556733257792 62-63 0.004805848144016719 64-65 4.409034994510751E-5 66-67 0.0017415688228317467 68-69 3.9681314950596765E-4 70-71 8.377166489570428E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0013006653233806715 80-81 0.0013888460232708867 82-83 0.01003055461251196 84-85 0.006767868716574004 86-87 0.0031524600210751873 88-89 2.4249692469809135E-4 90-91 4.4090349945107515E-4 92-93 1.1022587486276879E-4 94-95 2.2045174972553756E-5 96-97 0.001895885047639623 98-99 0.0012345297984630104 100-101 1.543162248078763E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2268070.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.22421474222341 #Duplication Level Percentage of deduplicated Percentage of total 1 76.23282677232388 43.623636496261796 2 13.654975984007105 15.62790556017452 3 4.3178497592730025 7.412566855478881 4 1.9236724084487427 4.403225719790439 5 1.017326616726336 2.9107858389263734 6 0.6245794200687963 2.14446401145541 7 0.42288490486652236 1.6939479625128615 8 0.3025256716834876 1.3849435201161016 9 0.20878654895024923 1.0752881681175124 >10 1.1866720893621534 12.236068697861523 >50 0.07688216839013687 3.027794783894884 >100 0.029317951852711882 2.8841781906579436 >500 0.0012361483977260197 0.45236708862739905 >1k 3.090370994313489E-4 0.4305710590442184 >5k 1.5451854971567446E-4 0.6922560470802311 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8721 0.3845119418712826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6937 0.3058547575692108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4695 0.2070041929922798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.409034994510751E-5 0.0 8 0.0 0.0 0.0 4.409034994510751E-5 0.0 9 0.0 0.0 0.0 1.3227104983532255E-4 0.0 10-11 0.0 0.0 0.0 1.3227104983532255E-4 0.0 12-13 0.0 0.0 0.0 1.7636139978043005E-4 0.0 14-15 0.0 0.0 0.0 1.7636139978043005E-4 0.0 16-17 0.0 0.0 0.0 1.7636139978043005E-4 0.0 18-19 0.0 0.0 0.0 1.7636139978043005E-4 2.2045174972553756E-5 20-21 0.0 0.0 0.0 1.984065747529838E-4 4.409034994510751E-5 22-23 0.0 0.0 0.0 3.7476797453341384E-4 4.409034994510751E-5 24-25 0.0 0.0 0.0 5.290841993412902E-4 4.409034994510751E-5 26-27 0.0 0.0 0.0 8.597618239295965E-4 4.409034994510751E-5 28-29 0.0 0.0 0.0 0.001543162248078763 4.409034994510751E-5 30-31 0.0 0.0 0.0 0.003306776245883064 4.409034994510751E-5 32-33 0.0 0.0 0.0 0.005687655142918869 4.409034994510751E-5 34-35 0.0 0.0 0.0 0.00787012746520169 4.409034994510751E-5 36-37 0.0 0.0 0.0 0.010802135736551342 4.409034994510751E-5 38-39 0.0 0.0 0.0 0.01331528568342247 4.409034994510751E-5 40-41 0.0 0.0 0.0 0.01648979087947021 4.409034994510751E-5 42-43 0.0 0.0 0.0 0.02182472322282822 4.409034994510751E-5 44-45 0.0 0.0 0.0 0.027666694590554965 4.409034994510751E-5 46-47 0.0 0.0 0.0 0.03331025938352873 4.409034994510751E-5 48-49 0.0 0.0 0.0 0.038997914526447595 4.409034994510751E-5 50-51 0.0 0.0 0.0 0.04642713849219821 4.409034994510751E-5 52-53 0.0 0.0 0.0 0.06712755779142619 4.409034994510751E-5 54-55 0.0 0.0 0.0 0.09287632215936897 4.409034994510751E-5 56-57 0.0 0.0 0.0 0.11549467168120914 4.409034994510751E-5 58-59 0.0 0.0 0.0 0.152354204235319 4.409034994510751E-5 60-61 0.0 0.0 0.0 0.19756885810402677 4.409034994510751E-5 62-63 0.0 0.0 0.0 0.23070275608777507 4.409034994510751E-5 64-65 0.0 0.0 0.0 0.25521699065725484 6.613552491766127E-5 66-67 0.0 0.0 0.0 0.32267522607326937 8.818069989021502E-5 68-69 0.0 0.0 0.0 0.3966147429312147 8.818069989021502E-5 70-71 0.0 0.0 0.0 0.42679458746864074 8.818069989021502E-5 72-73 0.0 0.0 0.0 0.487595180042944 8.818069989021502E-5 74-75 0.0 0.0 0.0 0.579876282478054 8.818069989021502E-5 76-77 0.0 0.0 0.0 0.6704378612653048 8.818069989021502E-5 78-79 0.0 0.0 0.0 0.7530411318874638 8.818069989021502E-5 80-81 0.0 0.0 0.0 0.8616356638022636 8.818069989021502E-5 82-83 0.0 0.0 0.0 0.9913053829908247 8.818069989021502E-5 84-85 0.0 0.0 0.0 1.135701279061052 8.818069989021502E-5 86-87 0.0 0.0 0.0 1.3467838294232541 8.818069989021502E-5 88-89 0.0 0.0 0.0 1.599972663983034 8.818069989021502E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7215 0.0 34.30646 1 GTATCAA 10745 0.0 31.65783 1 ATCAACG 13175 0.0 25.667257 3 TCAACGC 13220 0.0 25.54396 4 TATCAAC 13205 0.0 25.50329 2 CAACGCA 13530 0.0 24.926888 5 AACGCAG 13885 0.0 24.358 6 CGCACGA 55 0.0028770207 21.593029 28-29 GAGTACT 8260 0.0 21.562607 12-13 ACGCAGA 15725 0.0 21.205767 7 CGCAGAG 15770 0.0 21.115133 8 TACATGG 8450 0.0 21.079628 2 GTACATG 8525 0.0 21.06551 1 ACATGGG 8405 0.0 20.060452 3 GTACTTT 8770 0.0 19.983742 14-15 GCAGAGT 16520 0.0 19.811468 9 AGAGTAC 13245 0.0 19.420214 10-11 AGTACTT 8835 0.0 19.27226 12-13 CGCGGAA 315 0.0 18.848148 82-83 CCCGCGA 320 0.0 18.558556 20-21 >>END_MODULE