FastQCFastQC Report
Thu 26 May 2016
SRR936856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202192
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72910.33107921561789344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52570.2387166968184427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.15584472198609386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96200.034.3405151
GGTATCA63450.033.336921
ATCAACG117900.027.8599973
TCAACGC118200.027.7892864
TATCAAC118100.027.7323172
CAACGCA119850.027.4457425
AACGCAG121700.027.1816776
GTACATG90100.024.1095181
ACGCAGA136600.023.969397
TACATGG88550.023.6737382
CGCAGAG137850.023.6146858
ACATGGG87400.023.2237973
CGTATGA1703.1308664E-622.3695832
GAGTACT66900.022.12739612-13
GCAGAGT145050.021.7202369
GTACTTT71500.020.3731114-15
AGAGTAC119750.019.9593510-11
AGTACTT72400.019.65878312-13
CATGGGA54050.018.029144
CATGGGG40900.017.7821794
Produced by FastQC (version 0.11.5)