FastQCFastQC Report
Thu 26 May 2016
SRR936856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202192
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85210.3869326561898327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66870.30365199764598183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.20674854871873116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77000.032.0825581
GTATCAA111350.029.9504471
ATCAACG136800.024.1999423
TCAACGC138350.023.9288184
TATCAAC139450.023.8081862
CAACGCA139850.023.6721655
AACGCAG142600.023.3187546
GAGTACT86700.021.50612612-13
GTACATG82700.021.4843641
TACATGG81550.020.9674782
ACGCAGA160650.020.4917647
CGCAGAG161350.020.286948
ACATGGG79950.020.198923
GTACTTT91550.020.15741514-15
AGTACTT91500.019.49532512-13
AGAGTAC138400.019.1198910-11
GCAGAGT168700.018.980659
TATACCG1851.522834E-417.9718995
ACTTTTT110650.016.93529716-17
AGTACAT65450.016.7637042
Produced by FastQC (version 0.11.5)