##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202192 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09178672885925 31.0 31.0 34.0 31.0 34.0 2 32.22177766516271 33.0 31.0 34.0 30.0 34.0 3 32.35734531775613 34.0 31.0 34.0 31.0 34.0 4 36.03393119219396 37.0 35.0 37.0 35.0 37.0 5 35.867248632271846 37.0 35.0 37.0 35.0 37.0 6 35.95196740338717 37.0 35.0 37.0 35.0 37.0 7 35.905474182087666 37.0 35.0 37.0 35.0 37.0 8 35.85059885786526 37.0 35.0 37.0 35.0 37.0 9 37.54648640990431 39.0 37.0 39.0 35.0 39.0 10-11 37.45342140921409 39.0 37.0 39.0 34.5 39.0 12-13 37.345244193058555 39.0 37.0 39.0 34.0 39.0 14-15 38.5458463658028 40.0 38.0 41.0 34.0 41.0 16-17 38.544188472213136 40.0 38.0 41.0 34.0 41.0 18-19 38.66532686523246 40.0 38.0 41.0 34.0 41.0 20-21 38.66331341681379 40.0 38.0 41.0 34.0 41.0 22-23 38.57089095773665 40.0 38.0 41.0 34.0 41.0 24-25 38.48185921118595 40.0 38.0 41.0 34.0 41.0 26-27 38.28703673430836 40.0 38.0 41.0 34.0 41.0 28-29 38.134578638011575 40.0 38.0 41.0 33.0 41.0 30-31 38.13542029941077 40.0 37.5 41.0 34.0 41.0 32-33 38.02197719363252 40.0 37.0 41.0 33.0 41.0 34-35 37.904949023518384 40.0 37.0 41.0 33.0 41.0 36-37 37.7547647979831 40.0 37.0 41.0 33.0 41.0 38-39 37.59813880896852 40.0 37.0 41.0 33.0 41.0 40-41 37.28697974563526 40.0 36.5 41.0 31.5 41.0 42-43 37.0918988898334 39.0 36.0 41.0 31.0 41.0 44-45 36.88363298931247 39.0 36.0 41.0 31.0 41.0 46-47 36.54819334553935 39.0 36.0 41.0 30.5 41.0 48-49 36.198063338709794 39.0 35.0 40.0 30.0 41.0 50-51 35.92311183584356 39.0 35.0 40.0 29.0 41.0 52-53 35.66150703480895 38.5 35.0 40.0 28.0 41.0 54-55 35.27996582495986 38.0 34.5 40.0 27.0 41.0 56-57 35.07277453555366 38.0 34.0 40.0 27.0 41.0 58-59 34.746498034685445 38.0 34.0 40.0 26.5 41.0 60-61 34.37305716304482 37.5 33.5 40.0 26.0 41.0 62-63 33.99485944004883 37.0 33.0 39.5 26.0 40.5 64-65 33.659434781345134 36.0 33.0 39.0 25.0 40.0 66-67 33.318372103794765 36.0 32.5 39.0 24.5 40.0 68-69 32.86338906870972 35.5 32.0 38.5 23.0 40.0 70-71 32.418788870361894 35.0 31.0 38.0 22.0 40.0 72-73 32.001964179326784 35.0 31.0 37.0 21.5 39.0 74-75 31.510818538982974 34.5 31.0 37.0 20.0 39.0 76-77 30.073912946736705 33.5 29.5 35.0 19.5 37.5 78-79 30.758924971119683 34.0 30.0 36.0 19.5 38.0 80-81 30.624587910590904 34.0 30.0 35.5 18.0 37.0 82-83 30.269052153490705 34.0 30.0 35.0 17.5 37.0 84-85 29.81860755102189 34.0 29.5 35.0 12.0 36.0 86-87 29.428006504428314 34.0 29.0 35.0 7.5 36.0 88-89 29.034288790441522 34.0 29.0 35.0 4.5 35.5 90-91 28.56401440019762 33.0 29.0 35.0 2.0 35.0 92-93 28.053735550760333 33.0 27.5 35.0 2.0 35.0 94-95 27.469858441044195 33.0 26.5 34.0 2.0 35.0 96-97 26.663133368934226 32.0 25.0 34.0 2.0 35.0 98-99 25.7367148277716 32.0 24.5 34.0 2.0 35.0 100-101 22.42987623240844 28.0 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24019249360239314 1101 2 0.33624324520049953 1101 3 0.2633335901796414 1101 4 0.136997417449237 1101 5 0.0826297818476931 1101 6 0.17901893107112699 1101 7 0.2072945816243248 1101 8 0.2519777003555035 1101 9 0.23585984416348538 1101 10-11 0.2580180054356589 1101 12-13 0.27810377966607547 1101 14-15 0.4353890730975394 1101 16-17 0.40881165984231416 1101 18-19 0.2866781151691171 1101 20-21 0.22880670575264617 1101 22-23 0.2640685996156762 1101 24-25 0.3427959406414658 1101 26-27 0.2450141073855363 1101 28-29 0.2866431855978888 1101 30-31 0.4014894023907445 1101 32-33 0.5080932895633623 1101 34-35 0.2650195161233597 1101 36-37 0.3598248067039762 1101 38-39 0.4108951220747912 1101 40-41 0.4768675411761123 1101 42-43 0.3594390708111277 1101 44-45 0.29093957873529064 1101 46-47 0.14131099015427395 1101 48-49 0.4194156500291655 1101 50-51 0.4995919598333458 1101 52-53 0.4742902204920654 1101 54-55 0.2566387719965988 1101 56-57 0.3104005552276945 1101 58-59 0.43210258226316256 1101 60-61 0.3078312561094023 1101 62-63 0.48450057416109615 1101 64-65 0.3229153571717802 1101 66-67 0.2228013449290458 1101 68-69 -0.03447759442994425 1101 70-71 0.016756102262576178 1101 72-73 0.09820097410657525 1101 74-75 0.0887117587046582 1101 76-77 0.22742999527457286 1101 78-79 0.3305809266300379 1101 80-81 0.3243152498239503 1101 82-83 0.479105579850323 1101 84-85 0.24325996630709668 1101 86-87 -0.05335964669341209 1101 88-89 0.31023732813681093 1101 90-91 0.36142077647944504 1101 92-93 0.2326178267239527 1101 94-95 0.007943484255445554 1101 96-97 -0.06944651531867763 1101 98-99 0.24635853014049758 1101 100-101 0.7568466843850459 1102 1 -0.005180983712392617 1102 2 0.07504299302959083 1102 3 0.10201131982802991 1102 4 0.07985426978689958 1102 5 0.0016564834929013728 1102 6 0.1660972057340544 1102 7 0.1610751115899589 1102 8 0.23954811501395312 1102 9 0.24577585498242627 1102 10-11 0.2482743452969629 1102 12-13 0.26833419066398534 1102 14-15 0.2678342157571336 1102 16-17 0.2390675929763546 1102 18-19 0.291924896939598 1102 20-21 0.3150679670139098 1102 22-23 0.22682102472702326 1102 24-25 0.1429520222309577 1102 26-27 0.20749504204479763 1102 28-29 0.10343187044526303 1102 30-31 0.2371858211571478 1102 32-33 0.1965259660321621 1102 34-35 0.3053485584840132 1102 36-37 0.20074462042288843 1102 38-39 0.23315276346335878 1102 40-41 0.1870580929016441 1102 42-43 0.4077247153721757 1102 44-45 0.43220409381470404 1102 46-47 0.32194772169393815 1102 48-49 0.34206730845960465 1102 50-51 0.21822006875490274 1102 52-53 0.3050454113488428 1102 54-55 0.5148780496489636 1102 56-57 0.40228177052878067 1102 58-59 0.13298314680061907 1102 60-61 0.23752590561218057 1102 62-63 0.2049706492022736 1102 64-65 0.3165856640475013 1102 66-67 0.17152269607704085 1102 68-69 0.3667400453317171 1102 70-71 0.11323791138546824 1102 72-73 0.08862813942086945 1102 74-75 0.44447610092498024 1102 76-77 0.40863817862722485 1102 78-79 -0.064954224774187 1102 80-81 0.31644965285000026 1102 82-83 0.5561412498318141 1102 84-85 0.314175407707868 1102 86-87 0.20312358597492164 1102 88-89 0.31590405581256675 1102 90-91 0.36648300575160775 1102 92-93 0.30127694859977794 1102 94-95 0.42661887522202946 1102 96-97 0.83257085193571 1102 98-99 0.6614298090943933 1102 100-101 0.6827099630135613 1103 1 0.14717270733844856 1103 2 0.2370154918441969 1103 3 0.197505260705924 1103 4 0.10737255597645401 1103 5 0.0588718524425218 1103 6 0.09440446035709726 1103 7 0.17903366548466693 1103 8 0.20571808135647984 1103 9 0.13313292732655668 1103 10-11 0.23718234302722152 1103 12-13 0.21849081838747964 1103 14-15 0.32519979963263523 1103 16-17 0.30331741225205633 1103 18-19 0.29122765094468406 1103 20-21 0.21879807762495318 1103 22-23 0.23158106950000246 1103 24-25 0.28496070477027047 1103 26-27 0.29849905295821344 1103 28-29 0.27718681607251483 1103 30-31 0.3994482561546917 1103 32-33 0.3086986218631367 1103 34-35 0.3692161361565951 1103 36-37 0.1697665327579969 1103 38-39 0.22115675105751365 1103 40-41 0.3953896772901757 1103 42-43 0.3298873439196157 1103 44-45 0.33908832921490273 1103 46-47 0.2962631069670465 1103 48-49 0.2903412942659429 1103 50-51 0.1548122692383913 1103 52-53 0.19176062095016277 1103 54-55 0.32294534904499983 1103 56-57 0.0010009599511562328 1103 58-59 -0.11881632307478185 1103 60-61 -0.20455195741046595 1103 62-63 0.09659611850246819 1103 64-65 0.19346344778465152 1103 66-67 0.1749259483370622 1103 68-69 0.13570579710592057 1103 70-71 0.0548950560193191 1103 72-73 0.1443679819381103 1103 74-75 -0.05386162420307983 1103 76-77 0.261343990565134 1103 78-79 0.161636343360815 1103 80-81 0.11909114248458863 1103 82-83 0.4307860197095472 1103 84-85 0.09261499915436033 1103 86-87 0.06510074864233317 1103 88-89 0.24550514093565567 1103 90-91 0.05702296331054768 1103 92-93 -0.038512905965742306 1103 94-95 0.3660810941277326 1103 96-97 0.5892067371738356 1103 98-99 0.3458579818745946 1103 100-101 0.8058887284917233 1104 1 0.06553973700832927 1104 2 0.11738533537193518 1104 3 0.06372303153973746 1104 4 0.01994435987699461 1104 5 -0.045640813804396885 1104 6 0.05888999852684407 1104 7 0.10927330978815775 1104 8 0.10171027717611025 1104 9 0.12235243155900122 1104 10-11 0.20277884980146865 1104 12-13 0.1417576140874104 1104 14-15 0.2705369184598254 1104 16-17 0.2431216470304065 1104 18-19 0.2914744464891541 1104 20-21 0.19412202106796173 1104 22-23 0.16488408779008523 1104 24-25 0.249483553762488 1104 26-27 0.1460085555583106 1104 28-29 0.21852196053535522 1104 30-31 0.22795158692291295 1104 32-33 0.12400344350962911 1104 34-35 0.13845666659211986 1104 36-37 0.21560948528775015 1104 38-39 0.25409870940929835 1104 40-41 0.47219322803677954 1104 42-43 0.3102021928496512 1104 44-45 0.36733922894983806 1104 46-47 0.20595673070294396 1104 48-49 0.315941182333475 1104 50-51 0.47092277145760875 1104 52-53 0.29423460053803296 1104 54-55 0.5299681397390614 1104 56-57 0.39011960836661075 1104 58-59 0.051226390043858316 1104 60-61 0.17874212182839244 1104 62-63 0.32524091947254874 1104 64-65 0.2578018802637203 1104 66-67 0.13886503841937525 1104 68-69 0.24984815343982092 1104 70-71 0.1981478212953789 1104 72-73 0.2210605718533074 1104 74-75 0.04334997479885416 1104 76-77 0.3780075479965923 1104 78-79 0.5168025319825666 1104 80-81 0.30068388708423655 1104 82-83 0.4336187273092911 1104 84-85 0.4265627950952542 1104 86-87 0.24816863101996844 1104 88-89 0.360498650407159 1104 90-91 0.4658073300759291 1104 92-93 0.32087154319437516 1104 94-95 0.4565738301769855 1104 96-97 0.587751032115893 1104 98-99 0.26818656585115264 1104 100-101 0.9189712242748236 1105 1 0.01719166391231397 1105 2 0.17396372861394127 1105 3 0.1600769014499832 1105 4 0.12263508910300658 1105 5 0.06449081186343619 1105 6 0.11555839928323763 1105 7 0.12297179287252646 1105 8 0.14744895811185899 1105 9 0.14846782284396198 1105 10-11 0.17894564446047667 1105 12-13 0.17363266053624216 1105 14-15 0.18461628196632773 1105 16-17 0.030209491061420124 1105 18-19 0.14645087112643296 1105 20-21 0.11822008797079064 1105 22-23 0.046218676274072834 1105 24-25 0.1321694935978286 1105 26-27 0.25414762955439585 1105 28-29 0.18522805709937984 1105 30-31 0.14194589745709862 1105 32-33 0.22884692126786632 1105 34-35 0.12244338016178347 1105 36-37 0.1713935002835001 1105 38-39 0.13022648704945539 1105 40-41 0.15174748440130514 1105 42-43 0.2777336335479035 1105 44-45 0.23658225749625927 1105 46-47 0.15873106782650126 1105 48-49 0.0700608840700454 1105 50-51 0.007456738972329902 1105 52-53 0.16804592331818924 1105 54-55 0.3328890487323761 1105 56-57 0.2702339344250291 1105 58-59 0.3145031177339703 1105 60-61 0.4790567352502464 1105 62-63 0.4342588053694456 1105 64-65 0.27838862190912295 1105 66-67 0.3516898705842664 1105 68-69 0.3900683002594221 1105 70-71 0.26687000360600877 1105 72-73 0.22810985303182463 1105 74-75 0.07342342588513873 1105 76-77 0.14967458625391217 1105 78-79 0.18295537134805429 1105 80-81 0.23116526213481237 1105 82-83 0.13757183256853978 1105 84-85 -0.028625023337294664 1105 86-87 0.04169428316156498 1105 88-89 0.23125983322150745 1105 90-91 0.30318439550066145 1105 92-93 0.3730424583808727 1105 94-95 0.4723193648959416 1105 96-97 0.5629739865093306 1105 98-99 0.6633042775133191 1105 100-101 0.8744687015871477 1106 1 0.07988006852927754 1106 2 0.18868615160667446 1106 3 0.15411143756691104 1106 4 0.17395674230347424 1106 5 0.08692192514600805 1106 6 0.15728927886445376 1106 7 0.1700398031537702 1106 8 0.26050563641199886 1106 9 0.31815748771648344 1106 10-11 0.30128958520647586 1106 12-13 0.3313020042925885 1106 14-15 0.4126427908520469 1106 16-17 0.35311655383148377 1106 18-19 0.3084176010975028 1106 20-21 0.2859775329675287 1106 22-23 0.29129463931077026 1106 24-25 0.22281569647789468 1106 26-27 0.3387762130836762 1106 28-29 0.352566688082284 1106 30-31 0.2980806366775113 1106 32-33 0.31165342829618226 1106 34-35 0.22848094196881874 1106 36-37 0.3481437816987025 1106 38-39 0.2608845701599236 1106 40-41 0.3879629625289809 1106 42-43 0.38358096810066655 1106 44-45 -0.009250576714855185 1106 46-47 0.20466590242973837 1106 48-49 0.16172507194996655 1106 50-51 0.2099730552215533 1106 52-53 0.11128854875937577 1106 54-55 0.33553394783790225 1106 56-57 0.2903884895826323 1106 58-59 0.21671004550548645 1106 60-61 0.11893209153378592 1106 62-63 0.2047361636153724 1106 64-65 0.24865495801106619 1106 66-67 0.33251639903109975 1106 68-69 0.20772078377941483 1106 70-71 0.21522756623855344 1106 72-73 0.3345486193721925 1106 74-75 0.10226472655501695 1106 76-77 0.3339368252915058 1106 78-79 0.3473349366339171 1106 80-81 0.33786390851535586 1106 82-83 0.4538681617657758 1106 84-85 0.2907536379204565 1106 86-87 0.27654171776309866 1106 88-89 0.5031121890559405 1106 90-91 0.34419496881599443 1106 92-93 0.5795293199138278 1106 94-95 0.745289418011513 1106 96-97 0.9283203459230869 1106 98-99 1.095324055763971 1106 100-101 1.5484634918728197 1107 1 0.27596060563814717 1107 2 0.3608437191288303 1107 3 0.29644455903738987 1107 4 0.2136985618401468 1107 5 0.0886942675456126 1107 6 0.16615099086246232 1107 7 0.18833744855378853 1107 8 0.20985872413053386 1107 9 0.23856864777521736 1107 10-11 0.3104398690296506 1107 12-13 0.35806795427834714 1107 14-15 0.39824634430358685 1107 16-17 0.42403801115573003 1107 18-19 0.4465974799703858 1107 20-21 0.3770654122203112 1107 22-23 0.30773738885233826 1107 24-25 0.3941857186139117 1107 26-27 0.5303301906262092 1107 28-29 0.4507695065888697 1107 30-31 0.41960480730747207 1107 32-33 0.3425996516580838 1107 34-35 0.46166158793137413 1107 36-37 0.4729982173033491 1107 38-39 0.45771575929501296 1107 40-41 0.5564476316941693 1107 42-43 0.571399584270921 1107 44-45 0.505916612303345 1107 46-47 0.5278506395371565 1107 48-49 0.5153092070746368 1107 50-51 0.4677360025991959 1107 52-53 0.7263611300526236 1107 54-55 0.766868099400206 1107 56-57 0.7218293031360119 1107 58-59 0.7118792070399635 1107 60-61 0.6581760333518574 1107 62-63 0.6577910068733814 1107 64-65 0.7667604657148459 1107 66-67 0.9665240407052451 1107 68-69 0.6673484895968755 1107 70-71 0.617863432428905 1107 72-73 0.6859220751476443 1107 74-75 0.6627563214440109 1107 76-77 0.3945599212653832 1107 78-79 0.8438194742980194 1107 80-81 0.8720399446343201 1107 82-83 1.0578186735241637 1107 84-85 1.0421537591936811 1107 86-87 1.106787697847988 1107 88-89 1.0740256102027779 1107 90-91 1.0834286827719133 1107 92-93 1.041646049043507 1107 94-95 1.1550409540172417 1107 96-97 1.042433699858261 1107 98-99 1.1549218085565442 1107 100-101 1.603823987485793 1108 1 0.08647753718520335 1108 2 0.12594283603840495 1108 3 0.13882892653760592 1108 4 0.10259344860875075 1108 5 0.004604617159685631 1108 6 0.06760660616313174 1108 7 0.1816695199955447 1108 8 0.10552926760853865 1108 9 0.14171566515427259 1108 10-11 0.11238715467749927 1108 12-13 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-1.62342626091732 2316 46-47 -1.3877482748334202 2316 48-49 -1.22896462588389 2316 50-51 -1.4348359680904323 2316 52-53 -1.3688533853653908 2316 54-55 -1.4292624158471554 2316 56-57 -1.267675906625719 2316 58-59 -1.4361754360370185 2316 60-61 -1.2354291339516834 2316 62-63 -1.2481009299309633 2316 64-65 -1.3085382144246793 2316 66-67 -1.4680302194354518 2316 68-69 -1.3158545235123427 2316 70-71 -1.3334382461930971 2316 72-73 -1.523146201273434 2316 74-75 -1.1405278904081229 2316 76-77 -1.181877714971563 2316 78-79 -1.2829429975404203 2316 80-81 -1.38034137051309 2316 82-83 -1.3531941472230606 2316 84-85 -1.2030729451774818 2316 86-87 -1.1591653056116442 2316 88-89 -1.6858221497723562 2316 90-91 -1.6101409334182684 2316 92-93 -1.5878361845415299 2316 94-95 -1.6565732899934105 2316 96-97 -1.4313916405690428 2316 98-99 -1.573549351364555 2316 100-101 -2.335316975725913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 12.0 5 42.0 6 98.0 7 246.0 8 532.0 9 1169.0 10 1992.0 11 3233.0 12 4801.0 13 6495.0 14 8569.0 15 11581.0 16 14719.0 17 19846.0 18 17996.0 19 12177.0 20 11056.0 21 11693.0 22 12561.0 23 14147.0 24 16059.0 25 19489.0 26 24161.0 27 29864.0 28 37330.0 29 47027.0 30 58453.0 31 73001.0 32 93081.0 33 120636.0 34 162687.0 35 230680.0 36 345851.0 37 485592.0 38 290805.0 39 14501.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.590823764630116 19.597458156368532 15.020604448635128 22.79111363036623 2 15.590264769391105 20.73320754459937 39.81313334286966 23.86339434313986 3 19.484511973335575 24.682349575535206 29.962151227076745 25.870987224052477 4 13.226821276255658 17.202133147336834 36.99872672319216 32.57231885321534 5 13.800801313998745 37.36154900725796 34.236170113249294 14.601479565494 6 31.78619302949062 36.53323597579139 17.339496283702783 14.341074711015207 7 28.104652329706788 32.778097409529714 21.087400842889366 18.029849417874143 8 26.14753845259632 35.208601248210876 19.719806447394234 18.924053851798572 9 26.080310963177567 16.836104060049315 20.459133862200808 36.62445111457231 10-11 24.706201820731344 26.923083909123275 27.435505169394858 20.935209100750523 12-13 25.793753781982947 24.149515054704647 28.12392075120825 21.93281041210416 14-15 22.63939225643734 25.49312864507861 26.442989074584272 25.42449002389977 16-17 21.90774010622144 28.767655136336884 27.00874855598422 22.315856201457457 18-19 21.85908962936726 27.274518885831313 29.457514826217658 21.408876658583768 20-21 22.665565932457877 26.383813394672927 29.59256259687903 21.358058075990165 22-23 22.31114766113406 26.347326734651528 29.495558218601126 21.84596738561329 24-25 22.190158086281183 26.516364326587684 29.612541370871227 21.68093621625991 26-27 22.169628076797242 26.573315590377483 29.49518172490684 21.76187460791844 28-29 21.899914130894306 26.678545756063627 29.707600028880353 21.713940084161713 30-31 22.087084141618895 26.468400575426664 29.64398653705036 21.80052874590408 32-33 21.965911652925417 26.58944654853726 29.582218060707312 21.86242373783001 34-35 22.371232844365977 26.506748730355937 29.38158888961544 21.74042953566265 36-37 22.45151329382649 26.384351656335852 29.307994459959584 21.856140589878073 38-39 22.12509465944738 26.57387864743996 29.428614571707868 21.872412121404786 40-41 22.325417921010562 26.51388022008187 29.32743251518211 21.83326934372546 42-43 22.163803156128075 26.672697021876385 29.06099922259276 22.102500599402777 44-45 22.27517011668063 26.692890859676726 28.93983874959415 22.092100274048494 46-47 22.480269023780703 26.894885510274 28.559255593707068 22.065589872238224 48-49 22.54271148904828 27.069503814046797 28.27223918388458 22.115545513020344 50-51 22.488455657874244 27.15859557952393 28.182907607572744 22.170041155029082 52-53 22.571873780325962 27.24686236548251 28.011696610149095 22.16956724404243 54-55 22.50441832501435 27.082084577548187 28.018310846647342 22.395186250790122 56-57 22.476252578970946 27.41888573015955 27.915301451534646 22.18956023933486 58-59 22.69172750010444 27.190866538188384 27.931175721600514 22.186230240106656 60-61 22.529564396846343 27.013993648002714 28.359070396005343 22.097371559145596 62-63 22.420047843239825 27.023869853309794 28.333633034721768 22.22244926872861 64-65 22.456426270793532 27.148262159389734 28.21170500052902 22.18360656928771 66-67 22.395214040195867 27.28543362373345 27.989777455775304 22.32957488029538 68-69 22.272770411622254 27.182635096261038 28.224961617735804 22.3196328743809 70-71 22.36104678091709 27.048515561852348 28.201373184753294 22.389064472477273 72-73 22.45021815847771 27.047927659071096 27.98374885653262 22.518105325918572 74-75 22.366017574376404 27.38789075014537 27.76856824528473 22.477523430193497 76-77 22.4534190541407 27.320328691283397 27.771925012760036 22.454327241815868 78-79 22.500450688338454 27.277701666570547 27.71424497556293 22.507602669528065 80-81 22.39890089031336 27.4184363797859 27.652276461233722 22.53038626866702 82-83 22.417348734066707 27.34814601559039 27.749156010415998 22.4853492399269 84-85 22.438504606776192 27.27144752380245 27.6563691669431 22.633678702478253 86-87 22.42784380691082 27.17928493275654 27.700835238827626 22.69203602150501 88-89 22.58240957322896 27.21313031272748 27.582384597534855 22.62207551650871 90-91 22.43024011414243 27.184865230108063 27.762774837988687 22.622119817760826 92-93 22.324756424507942 27.115097139577294 27.74960130633478 22.81054512957998 94-95 22.28189510312795 27.10595331968178 27.722180382587077 22.889971194603195 96-97 22.315349384656884 27.135287391709873 27.668802726018605 22.880560497614645 98-99 22.175523504457857 27.136770960385583 27.51176335427926 23.175942180877296 100-101 21.987445385474167 27.28513669780534 27.542976974427358 23.18444094229314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 393.5 2 316.0 3 354.5 4 316.5 5 305.0 6 533.0 7 1089.0 8 1824.0 9 2239.5 10 2160.0 11 2040.5 12 2118.5 13 2327.5 14 2584.0 15 2724.5 16 2933.5 17 3172.5 18 3375.5 19 3678.5 20 4047.0 21 4616.5 22 5483.5 23 6729.0 24 8480.0 25 10675.5 26 13393.0 27 16556.5 28 19395.0 29 22357.5 30 26150.0 31 29818.0 32 33497.0 33 37890.0 34 42971.5 35 47166.5 36 51820.5 37 56719.0 38 59836.0 39 62506.5 40 65579.5 41 69056.0 42 72068.5 43 76025.0 44 80228.5 45 82882.5 46 84261.5 47 85813.0 48 89727.5 49 88085.5 50 81368.5 51 76999.0 52 74586.5 53 72883.0 54 70052.0 55 65548.0 56 59743.5 57 54011.5 58 49276.5 59 44347.5 60 37947.5 61 30725.5 62 23996.0 63 18714.5 64 14331.5 65 10770.5 66 8138.0 67 6379.0 68 4539.0 69 2925.0 70 1973.5 71 1331.5 72 947.0 73 695.5 74 510.0 75 350.5 76 214.5 77 119.5 78 77.0 79 45.0 80 21.5 81 14.5 82 12.5 83 8.0 84 5.0 85 3.5 86 2.0 87 0.5 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021932692517273696 2 0.003950609211185946 3 3.1786510894599564E-4 4 0.0 5 0.0026791487754019632 6 0.0 7 2.270465063899969E-4 8 0.0 9 9.081860255599875E-5 10-11 0.0 12-13 0.006334597528280913 14-15 0.0035873348009619506 16-17 0.0 18-19 5.449116153359925E-4 20-21 0.0011579371825889842 22-23 0.0015893255447299783 24-25 3.8597906086299466E-4 26-27 1.5893255447299782E-4 28-29 1.3622790383399812E-4 30-31 0.0 32-33 1.1352325319499845E-4 34-35 0.0 36-37 0.0019071906536759736 38-39 0.009967341630520862 40-41 5.222069646969927E-4 42-43 0.0 44-45 0.001226051134505983 46-47 3.405697595849953E-4 48-49 1.5893255447299782E-4 50-51 0.0014076883396179805 52-53 7.038441698089902E-4 54-55 0.0 56-57 2.4975115702899656E-4 58-59 1.816372051119975E-4 60-61 0.002270465063899969 62-63 0.0 64-65 2.270465063899969E-4 66-67 2.2704650638999686E-5 68-69 1.3622790383399812E-4 70-71 5.449116153359925E-4 72-73 3.8597906086299466E-4 74-75 0.002974309233708959 76-77 1.816372051119975E-4 78-79 2.270465063899969E-4 80-81 0.002088827858787971 82-83 1.1352325319499845E-4 84-85 0.0025202162209289653 86-87 3.405697595849953E-4 88-89 0.0023158743651779685 90-91 0.0014076883396179805 92-93 0.0 94-95 2.2704650638999686E-5 96-97 2.7245580766799624E-4 98-99 6.357302178919913E-4 100-101 0.0024293976183729664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2202192.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.83152980697972 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51402280126663 44.24922990283377 2 13.563522509770266 15.687985126228387 3 4.279041884234281 7.423906148202283 4 1.8859781024835764 4.3627599539636 5 0.9958548003844908 2.879590328592976 6 0.6061192276520504 2.1031681308325845 7 0.4050994545297098 1.6399264825598294 8 0.2700090550813429 1.2492029373672886 9 0.2257296516936356 1.1748861972215672 >10 1.1493003763238534 11.886828603319662 >50 0.0747431515948347 2.942685404478963 >100 0.02900448175799618 2.8654928304489315 >500 0.0011021522591674548 0.4138947312947012 >1k 3.1490064547639045E-4 0.42805392846029366 >5k 1.5745032273819522E-4 0.692389294195293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8521 0.3869326561898327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6687 0.30365199764598183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4553 0.20674854871873116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.540930127799937E-5 2 0.0 0.0 0.0 0.0 4.540930127799937E-5 3 0.0 0.0 0.0 0.0 4.540930127799937E-5 4 0.0 0.0 0.0 0.0 4.540930127799937E-5 5 0.0 0.0 0.0 4.540930127799937E-5 4.540930127799937E-5 6 0.0 0.0 0.0 4.540930127799937E-5 4.540930127799937E-5 7 0.0 0.0 0.0 4.540930127799937E-5 4.540930127799937E-5 8 0.0 0.0 0.0 4.540930127799937E-5 4.540930127799937E-5 9 0.0 0.0 0.0 9.081860255599875E-5 4.540930127799937E-5 10-11 0.0 0.0 0.0 9.081860255599875E-5 4.540930127799937E-5 12-13 0.0 0.0 0.0 9.081860255599875E-5 4.540930127799937E-5 14-15 0.0 0.0 0.0 9.081860255599875E-5 4.540930127799937E-5 16-17 0.0 0.0 0.0 1.5893255447299782E-4 4.540930127799937E-5 18-19 0.0 0.0 0.0 1.816372051119975E-4 4.540930127799937E-5 20-21 0.0 0.0 0.0 2.0434185575099716E-4 4.540930127799937E-5 22-23 0.0 0.0 0.0 3.859790608629947E-4 4.540930127799937E-5 24-25 0.0 0.0 0.0 7.94662772364989E-4 4.540930127799937E-5 26-27 0.0 0.0 0.0 0.001226051134505983 4.540930127799937E-5 28-29 0.0 0.0 0.0 0.0022023511119829697 4.540930127799937E-5 30-31 0.0 0.0 0.0 0.003996018512463945 4.540930127799937E-5 32-33 0.0 0.0 0.0 0.007242783553840901 4.540930127799937E-5 34-35 0.0 0.0 0.0 0.010376025342022858 4.540930127799937E-5 36-37 0.0 0.0 0.0 0.013713608985955812 4.540930127799937E-5 38-39 0.0 0.0 0.0 0.01673332752094277 4.540930127799937E-5 40-41 0.0 0.0 0.0 0.021524008805771704 4.540930127799937E-5 42-43 0.0 0.0 0.0 0.02828999469619361 9.081860255599875E-5 44-45 0.0 0.0 0.0 0.03451106897127952 1.3622790383399812E-4 46-47 0.0 0.0 0.0 0.04084566649956044 1.3622790383399812E-4 48-49 0.0 0.0 0.0 0.045931508242696366 1.3622790383399812E-4 50-51 0.0 0.0 0.0 0.05396895456890226 1.3622790383399812E-4 52-53 0.0 0.0 0.0 0.07365388667291499 1.3622790383399812E-4 54-55 0.0 0.0 0.0 0.10033185117373962 1.3622790383399812E-4 56-57 0.0 0.0 0.0 0.12414902969405028 1.3622790383399812E-4 58-59 0.0 0.0 0.0 0.16406380551741173 1.3622790383399812E-4 60-61 0.0 0.0 0.0 0.20940499284349412 1.3622790383399812E-4 62-63 0.0 0.0 0.0 0.2406011828214797 1.3622790383399812E-4 64-65 0.0 0.0 0.0 0.2651222055115994 1.3622790383399812E-4 66-67 0.0 0.0 0.0 0.3369824247840334 1.3622790383399812E-4 68-69 0.0 0.0 0.0 0.41483667182516326 1.3622790383399812E-4 70-71 0.0 0.0 0.0 0.44646425016528984 1.3622790383399812E-4 72-73 0.0 0.0 0.0 0.5093334277846799 1.3622790383399812E-4 74-75 0.0 0.0 0.0 0.6047610744203957 1.3622790383399812E-4 76-77 0.0 0.0 0.0 0.7001660164054724 1.3622790383399812E-4 78-79 0.0 0.0 0.0 0.7790873820266353 1.5893255447299782E-4 80-81 0.0 0.0 0.0 0.8902720562058167 1.816372051119975E-4 82-83 0.0 0.0 0.0 1.0212097764409278 1.816372051119975E-4 84-85 0.0 0.0 0.0 1.1649302149857959 1.816372051119975E-4 86-87 0.0 0.0 0.0 1.384688528520674 1.816372051119975E-4 88-89 0.0 0.0 0.0 1.6450427573980835 1.816372051119975E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7700 0.0 32.082558 1 GTATCAA 11135 0.0 29.950447 1 ATCAACG 13680 0.0 24.199942 3 TCAACGC 13835 0.0 23.928818 4 TATCAAC 13945 0.0 23.808186 2 CAACGCA 13985 0.0 23.672165 5 AACGCAG 14260 0.0 23.318754 6 GAGTACT 8670 0.0 21.506126 12-13 GTACATG 8270 0.0 21.484364 1 TACATGG 8155 0.0 20.967478 2 ACGCAGA 16065 0.0 20.491764 7 CGCAGAG 16135 0.0 20.28694 8 ACATGGG 7995 0.0 20.19892 3 GTACTTT 9155 0.0 20.157415 14-15 AGTACTT 9150 0.0 19.495325 12-13 AGAGTAC 13840 0.0 19.11989 10-11 GCAGAGT 16870 0.0 18.98065 9 TATACCG 185 1.522834E-4 17.971899 5 ACTTTTT 11065 0.0 16.935297 16-17 AGTACAT 6545 0.0 16.763704 2 >>END_MODULE