##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936855_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174311 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88229658483974 33.0 31.0 34.0 30.0 34.0 2 31.90908777988767 33.0 31.0 34.0 30.0 34.0 3 31.880609944294967 33.0 31.0 34.0 30.0 34.0 4 35.41399567439806 37.0 35.0 37.0 33.0 37.0 5 35.484169100056796 37.0 35.0 37.0 33.0 37.0 6 35.66105982984436 37.0 35.0 37.0 33.0 37.0 7 35.659562506095426 37.0 35.0 37.0 33.0 37.0 8 35.65912076690513 37.0 35.0 37.0 33.0 37.0 9 37.19073380337443 39.0 37.0 39.0 34.0 39.0 10-11 37.09195059405316 39.0 37.0 39.0 33.5 39.0 12-13 37.058980787213656 39.0 37.0 39.0 33.0 39.0 14-15 37.9891716529651 40.0 38.0 41.0 33.0 41.0 16-17 37.98334012196591 40.0 38.0 41.0 33.0 41.0 18-19 38.045464715365064 40.0 38.0 41.0 33.5 41.0 20-21 37.96905530918875 40.0 37.5 41.0 33.0 41.0 22-23 37.84650997355302 40.0 37.0 41.0 33.0 41.0 24-25 37.53104795451807 39.5 36.5 41.0 32.5 41.0 26-27 37.410507655856485 39.0 36.0 41.0 32.5 41.0 28-29 37.5275628044128 39.5 36.5 41.0 33.0 41.0 30-31 37.43234792984952 39.0 36.0 41.0 33.0 41.0 32-33 37.06059571685091 39.0 35.0 41.0 33.0 41.0 34-35 36.74197841788528 39.0 35.0 41.0 31.5 41.0 36-37 36.43567244752196 38.5 35.0 40.5 30.5 41.0 38-39 36.15279299642593 38.0 35.0 40.0 30.0 41.0 40-41 36.10684064688975 38.0 35.0 40.0 30.5 41.0 42-43 35.734698326554266 38.0 35.0 40.0 30.0 41.0 44-45 35.26862905955447 38.0 35.0 40.0 28.0 41.0 46-47 34.565538032596905 38.0 35.0 40.0 23.5 41.0 48-49 33.8721738731348 38.0 34.0 40.0 18.5 41.0 50-51 33.08101898331144 37.5 33.0 39.5 14.0 40.5 52-53 33.41323840721469 38.0 33.5 39.5 12.5 40.5 54-55 33.73391524344419 38.0 34.0 40.0 8.5 41.0 56-57 33.46180103378444 38.0 33.5 40.0 7.5 41.0 58-59 33.26126291513444 38.0 33.5 40.0 2.0 41.0 60-61 32.694046847301664 38.0 32.0 40.0 2.0 41.0 62-63 32.50867988824572 37.0 32.5 40.0 2.0 41.0 64-65 32.20570130399114 36.5 32.0 39.5 2.0 41.0 66-67 31.526180218115897 36.0 31.0 39.0 2.0 40.5 68-69 30.977838461141296 35.5 31.0 38.5 2.0 39.5 70-71 30.323232612973364 35.0 29.5 37.5 2.0 39.0 72-73 29.861640975038867 35.0 29.5 37.0 2.0 39.0 74-75 29.513774231115647 35.0 29.0 36.5 2.0 38.0 76-77 29.096875699181346 34.0 29.0 36.0 2.0 37.0 78-79 28.573879445359157 34.0 29.0 35.5 2.0 37.0 80-81 28.015790741834998 34.0 26.5 35.0 2.0 36.5 82-83 27.53242480394238 34.0 26.5 35.0 2.0 36.0 84-85 27.17113664656849 34.0 26.0 35.0 2.0 36.0 86-87 27.11924663389 34.0 26.0 35.0 2.0 35.0 88-89 26.544110813431168 34.0 24.5 35.0 2.0 35.0 90-91 26.067408253064926 33.0 23.5 35.0 2.0 35.0 92-93 25.700893804751278 33.0 20.0 35.0 2.0 35.0 94-95 25.137656258067477 33.0 18.0 35.0 2.0 35.0 96-97 24.65999563997682 32.5 6.0 35.0 2.0 35.0 98-99 24.202287864793387 32.5 2.0 35.0 2.0 35.0 100-101 22.158380710339564 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7027947601352444 1101 2 0.5363429504993604 1101 3 0.6046085078122054 1101 4 0.588796181336761 1101 5 0.8405940507394831 1101 6 0.43173516601845563 1101 7 0.529261934104575 1101 8 0.6271034146808745 1101 9 0.6907321828718551 1101 10-11 0.5994657453562198 1101 12-13 0.7354763909200344 1101 14-15 0.9866062983786179 1101 16-17 0.7610856673735 1101 18-19 1.0338423852620267 1101 20-21 1.006305032915158 1101 22-23 0.9605472889295612 1101 24-25 1.2067648923625995 1101 26-27 0.8744753041407733 1101 28-29 0.797555134525183 1101 30-31 1.0294907290437436 1101 32-33 0.9705236271794817 1101 34-35 1.2468556347972637 1101 36-37 1.3641395999882917 1101 38-39 1.4007781252113745 1101 40-41 1.5107651440152807 1101 42-43 1.1540377822838508 1101 44-45 1.0240029034879754 1101 46-47 1.6146656657579115 1101 48-49 1.7183269293271834 1101 50-51 1.2047569162733254 1101 52-53 1.5973650681255833 1101 54-55 1.8115116551636206 1101 56-57 1.7561834578076372 1101 58-59 1.645785785438889 1101 60-61 1.9091812240561836 1101 62-63 1.7407814746554635 1101 64-65 1.4132152624315566 1101 66-67 1.8404331345985163 1101 68-69 1.4622079458538089 1101 70-71 1.1238474646592351 1101 72-73 1.125819327561178 1101 74-75 0.7162287640560692 1101 76-77 1.169322648141712 1101 78-79 0.9120063131024629 1101 80-81 1.0045330099898706 1101 82-83 1.3750587331657904 1101 84-85 1.5684778334122171 1101 86-87 1.6422916373267213 1101 88-89 1.7279645124670537 1101 90-91 1.9952978455891461 1101 92-93 1.6843473526763866 1101 94-95 1.8233186712408198 1101 96-97 1.9456987663016747 1101 98-99 2.217137133994651 1101 100-101 2.5182457908016787 1102 1 0.06815423725942438 1102 2 -0.026620012463602905 1102 3 0.08107909604749963 1102 4 -0.08150156739236536 1102 5 0.23166158886584043 1102 6 0.05795157850938182 1102 7 -0.15621373764560786 1102 8 0.03785141758573474 1102 9 -0.6555278026038138 1102 10-11 -0.2777673701121941 1102 12-13 -0.2609651486587552 1102 14-15 0.23115967528493542 1102 16-17 -0.21302471752119345 1102 18-19 0.1416855225169229 1102 20-21 -0.2317850179199894 1102 22-23 -0.11468873140449887 1102 24-25 -0.0158567488864918 1102 26-27 0.14760529687860924 1102 28-29 0.3454127961083344 1102 30-31 0.22008622650199072 1102 32-33 0.2558540556471627 1102 34-35 0.7419899848335731 1102 36-37 0.6146116406564062 1102 38-39 0.2845108775715772 1102 40-41 0.3806997845381588 1102 42-43 -0.027480010018258838 1102 44-45 0.6740392143085998 1102 46-47 0.5250868712771535 1102 48-49 0.5663661450134541 1102 50-51 0.27687020603367074 1102 52-53 0.7446054457581113 1102 54-55 1.1368566079595581 1102 56-57 0.9841064788679077 1102 58-59 0.5152846961142714 1102 60-61 0.6979248696625788 1102 62-63 0.4147466162676636 1102 64-65 0.22842949627323605 1102 66-67 0.16857402058253967 1102 68-69 0.09877294222272681 1102 70-71 0.2152781109918429 1102 72-73 0.09034365581099735 1102 74-75 0.24959840821003 1102 76-77 0.39463129011702236 1102 78-79 0.1359714547146602 1102 80-81 0.389100911224439 1102 82-83 0.4749861115245402 1102 84-85 0.32461436209776195 1102 86-87 0.2580142226571489 1102 88-89 0.540745921326895 1102 90-91 0.9385803437736051 1102 92-93 0.7747249852108844 1102 94-95 1.1513506247629692 1102 96-97 1.5160328258514255 1102 98-99 1.8553361172916745 1102 100-101 2.340286458920776 1103 1 0.396768394786843 1103 2 0.593153998835831 1103 3 0.7769248919929943 1103 4 0.6740650660406686 1103 5 0.7235193489190195 1103 6 0.19434240602682706 1103 7 0.5695423274923073 1103 8 0.5419391543753704 1103 9 0.776210316372584 1103 10-11 0.4409597817655069 1103 12-13 0.4304439573565304 1103 14-15 0.3630971960559535 1103 16-17 0.5326382934459062 1103 18-19 0.9957067920416875 1103 20-21 0.9867925826117485 1103 22-23 0.7021773370567885 1103 24-25 1.057622812429564 1103 26-27 0.830323808011876 1103 28-29 1.066649087234957 1103 30-31 1.2233597392969386 1103 32-33 1.7929760230782108 1103 34-35 1.4323023809784772 1103 36-37 1.4402015376321486 1103 38-39 1.2005793388543964 1103 40-41 1.3295033737704642 1103 42-43 1.8246801760900908 1103 44-45 1.3958903276352856 1103 46-47 1.342119107900615 1103 48-49 1.6646546129429964 1103 50-51 1.4017123462796022 1103 52-53 1.6545423018564875 1103 54-55 1.4284817325856736 1103 56-57 1.9494770338695702 1103 58-59 2.380405998872668 1103 60-61 2.498373523700458 1103 62-63 2.381396666118114 1103 64-65 2.3074923078385368 1103 66-67 2.2410399561092937 1103 68-69 2.1505841751904917 1103 70-71 2.194573557984203 1103 72-73 2.003670342417845 1103 74-75 1.849415623231632 1103 76-77 1.9765521940718251 1103 78-79 2.0512090751865806 1103 80-81 1.8958805722413885 1103 82-83 1.933543771876817 1103 84-85 2.6632152261722055 1103 86-87 2.2218061801180973 1103 88-89 2.3668010891567413 1103 90-91 2.4997966947207644 1103 92-93 2.1031065073112494 1103 94-95 1.902048531044123 1103 96-97 2.541639339643382 1103 98-99 3.196944625101576 1103 100-101 3.321708858389041 1104 1 0.7137766722799448 1104 2 0.8524497549782595 1104 3 0.8778643217656956 1104 4 0.7345039045638586 1104 5 0.8339187708083884 1104 6 0.7766246291249743 1104 7 0.8437178629015847 1104 8 0.7799170810604323 1104 9 0.9606144682580151 1104 10-11 0.7504132044432126 1104 12-13 0.8663262330101915 1104 14-15 1.2749353250797384 1104 16-17 1.0859492906867345 1104 18-19 1.0289974240217106 1104 20-21 1.047182150342671 1104 22-23 1.1582934847377757 1104 24-25 0.8670433879124104 1104 26-27 1.2308132312151372 1104 28-29 1.1517397454927405 1104 30-31 1.0781915616593096 1104 32-33 1.144260348396827 1104 34-35 1.4138094102781693 1104 36-37 1.5209727897672138 1104 38-39 1.7963781826331413 1104 40-41 2.3689692783822096 1104 42-43 2.0133544633059586 1104 44-45 2.6105303223797307 1104 46-47 2.061406980716278 1104 48-49 1.984799797544234 1104 50-51 1.8621643236807301 1104 52-53 2.5241882090960104 1104 54-55 2.408231436960925 1104 56-57 2.60100114371059 1104 58-59 2.7360097303140023 1104 60-61 2.914169739703617 1104 62-63 2.837250036240306 1104 64-65 3.1304130804045585 1104 66-67 3.216043240828771 1104 68-69 2.94706295590262 1104 70-71 2.6946556759713225 1104 72-73 2.4577171168233036 1104 74-75 2.6593453302346575 1104 76-77 3.0632359412798174 1104 78-79 2.0084748746873053 1104 80-81 2.023984632154672 1104 82-83 2.8938643604985472 1104 84-85 2.88347209123593 1104 86-87 3.242384947691349 1104 88-89 3.133290380971218 1104 90-91 3.086562559915876 1104 92-93 3.1179534393832498 1104 94-95 2.052718613819053 1104 96-97 1.97082078754773 1104 98-99 1.3224701802191703 1104 100-101 1.4531797557744035 1105 1 0.3388746602600854 1105 2 0.23068335832291353 1105 3 0.5091028898346757 1105 4 0.5331712680339464 1105 5 0.3909477752505097 1105 6 0.3863058415629155 1105 7 0.48958930267163936 1105 8 0.09707151011713222 1105 9 0.20349400836776255 1105 10-11 0.4238519292929581 1105 12-13 0.34901502318526667 1105 14-15 0.2880003890985492 1105 16-17 0.560537741170144 1105 18-19 0.6359023103556609 1105 20-21 1.0515262841289186 1105 22-23 0.8027930927858549 1105 24-25 0.950071869751973 1105 26-27 0.8877573127411367 1105 28-29 0.723238308335695 1105 30-31 0.10141471295275295 1105 32-33 0.4677839961626944 1105 34-35 0.380820123630663 1105 36-37 0.60661428441054 1105 38-39 0.560717090393787 1105 40-41 0.5263706371488723 1105 42-43 0.9646217745157841 1105 44-45 -0.2118827949445432 1105 46-47 -0.022732031564885347 1105 48-49 0.11626700423354208 1105 50-51 0.5335126382874691 1105 52-53 0.2859141040528925 1105 54-55 0.10149639645922548 1105 56-57 0.20839596994524356 1105 58-59 -0.08101404810251012 1105 60-61 -0.2611960820889081 1105 62-63 -0.14602668181561285 1105 64-65 -0.20098226843264655 1105 66-67 -0.12266854385896764 1105 68-69 0.2399163658843264 1105 70-71 -0.22679062661555704 1105 72-73 -0.21441510162456012 1105 74-75 -0.3885707920543453 1105 76-77 -0.10536870988297764 1105 78-79 -0.2276108321326582 1105 80-81 -0.35640470675308933 1105 82-83 -0.32732717333996675 1105 84-85 -0.1838456511210076 1105 86-87 0.19760444076686312 1105 88-89 0.15968181028922146 1105 90-91 0.4487587971774367 1105 92-93 0.885068673247897 1105 94-95 1.441469593698855 1105 96-97 1.408696408138269 1105 98-99 1.5316745178204236 1105 100-101 1.970458302939285 1106 1 0.23737901326254374 1106 2 0.06675747098010731 1106 3 0.38937671973273424 1106 4 0.30357829236611167 1106 5 0.3668388385736705 1106 6 0.32409883211281 1106 7 0.2584337107377195 1106 8 0.3753660260781402 1106 9 0.6936346438317074 1106 10-11 0.5215898562220431 1106 12-13 0.4791400324865478 1106 14-15 0.6481834384325751 1106 16-17 0.5649651539240708 1106 18-19 0.6491845486174341 1106 20-21 0.5689941016748676 1106 22-23 0.7442321879566194 1106 24-25 0.6122305125081269 1106 26-27 0.8018320206807132 1106 28-29 0.7309211716439847 1106 30-31 0.6131715400976248 1106 32-33 0.8160800003296771 1106 34-35 0.33799699690992924 1106 36-37 0.399418808556689 1106 38-39 0.09648984134251748 1106 40-41 0.1500219504906326 1106 42-43 0.30382111970515524 1106 44-45 0.3546586143865085 1106 46-47 0.027755355889539146 1106 48-49 -0.16688278369710474 1106 50-51 -0.9192926299616531 1106 52-53 -0.2394226025473003 1106 54-55 0.2350256769116399 1106 56-57 0.30857083414644393 1106 58-59 0.24101512073445264 1106 60-61 -0.04276465117887085 1106 62-63 0.32785530345114466 1106 64-65 0.034117069315698245 1106 66-67 -0.6547021773138368 1106 68-69 -0.2893454839556888 1106 70-71 -0.45063540673313085 1106 72-73 -0.4844128693156087 1106 74-75 -0.9591861652219933 1106 76-77 -0.506030961538606 1106 78-79 -0.49264171240648835 1106 80-81 -0.23937590996761315 1106 82-83 -0.32929904624717565 1106 84-85 0.015518140827797566 1106 86-87 -0.1423948151301495 1106 88-89 -0.09645703932756433 1106 90-91 0.011077422075125298 1106 92-93 0.15992171290858437 1106 94-95 0.3792625842487496 1106 96-97 0.24865845888609428 1106 98-99 0.29542182044710685 1106 100-101 0.9812291936305577 1107 1 0.2791836490241302 1107 2 0.5620163511727654 1107 3 0.5421085078122054 1107 4 0.39610538447929855 1107 5 0.21327923592466647 1107 6 0.25019756781419034 1107 7 0.28108572759503403 1107 8 0.4179984764092666 1107 9 0.6792984006047362 1107 10-11 0.8048529507434168 1107 12-13 0.6946164021433887 1107 14-15 0.4901837394560573 1107 16-17 0.6635127156338854 1107 18-19 0.7459635973832377 1107 20-21 0.9951869072137001 1107 22-23 1.0350773113762486 1107 24-25 0.9476459249102973 1107 26-27 0.9455331043652393 1107 28-29 0.8514622613489706 1107 30-31 0.9489632318495893 1107 32-33 0.8633406866632072 1107 34-35 1.015619663977965 1107 36-37 1.1184886460040104 1107 38-39 1.2624854765020572 1107 40-41 1.4372171642173015 1107 42-43 1.6604210595004574 1107 44-45 1.547501781153521 1107 46-47 2.083275373950954 1107 48-49 2.0488193535696055 1107 50-51 1.9473915964080035 1107 52-53 1.7006549109987503 1107 54-55 2.0858873566226563 1107 56-57 2.4802294735203105 1107 58-59 2.341387637290744 1107 60-61 2.522256420464714 1107 62-63 2.71310891573291 1107 64-65 2.58310864065826 1107 66-67 2.3901315072135567 1107 68-69 2.4494413914205886 1107 70-71 2.580953244681684 1107 72-73 2.5026431210516336 1107 74-75 2.5105609750549505 1107 76-77 2.5679267446624756 1107 78-79 2.935470117816269 1107 80-81 3.065805456678987 1107 82-83 3.217265523289246 1107 84-85 3.4119472497982954 1107 86-87 3.036055666507419 1107 88-89 2.991046723465935 1107 90-91 2.616755477463446 1107 92-93 3.4088493167616853 1107 94-95 3.8337704108592234 1107 96-97 3.443138440824683 1107 98-99 3.182274619965469 1107 100-101 3.5121781701507224 1108 1 0.14915491339194276 1108 2 0.48142596454789555 1108 3 0.5988613813754249 1108 4 0.7443943081992543 1108 5 0.4338252129361635 1108 6 0.5132592188068799 1108 7 0.5390533347005686 1108 8 0.2290616948000732 1108 9 0.3671902501345201 1108 10-11 0.3153022715375684 1108 12-13 0.25505919211203576 1108 14-15 0.277474753040174 1108 16-17 0.3291997584761006 1108 18-19 0.43901479905512986 1108 20-21 0.546747774507331 1108 22-23 0.7066094430376921 1108 24-25 0.48826765949755213 1108 26-27 0.6655566153370245 1108 28-29 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-3.055531770725537 2316 62-63 -2.365938647528573 2316 64-65 -2.1700544333811003 2316 66-67 -1.9887637598904746 2316 68-69 -1.340038458626271 2316 70-71 -2.3288698574738937 2316 72-73 -2.3078067383672902 2316 74-75 -3.3034034110743846 2316 76-77 -3.3966058232850393 2316 78-79 -3.6844045307310616 2316 80-81 -3.2557444496003 2316 82-83 -3.514371074648899 2316 84-85 -3.675476602122977 2316 86-87 -3.184041568731569 2316 88-89 -3.3354820956530915 2316 90-91 -3.6519593772694527 2316 92-93 -4.122576409573835 2316 94-95 -3.8619301514650495 2316 96-97 -3.954676695070347 2316 98-99 -3.9273200379539297 2316 100-101 -3.6493436199148768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 550.0 3 125.0 4 114.0 5 87.0 6 94.0 7 147.0 8 173.0 9 244.0 10 350.0 11 512.0 12 842.0 13 1113.0 14 1346.0 15 1940.0 16 2502.0 17 3581.0 18 2822.0 19 1528.0 20 1325.0 21 1380.0 22 1421.0 23 1481.0 24 1781.0 25 2028.0 26 2252.0 27 2664.0 28 2983.0 29 3627.0 30 4480.0 31 5442.0 32 6833.0 33 8780.0 34 12374.0 35 18711.0 36 22402.0 37 29117.0 38 24089.0 39 3069.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.65642167865487 21.52710405915074 15.463923443877405 14.352550818316983 2 14.396067012411196 27.263851318366385 33.98347088452018 24.35661078470224 3 27.07643198741539 25.614159983006186 34.91425586028327 12.395152169295159 4 10.643810355425597 20.021485238951474 43.22439436328437 26.110310042338558 5 8.265136766420298 43.60732560343194 35.49092913874197 12.636608491405795 6 21.80894133861659 40.72874493927125 16.78697562236196 20.675338099750192 7 19.361953886980597 41.10537985226372 21.99386538306892 17.538800877686768 8 23.178918232926193 25.81011938601478 21.29825830792289 29.712704073136138 9 20.036760482481334 24.402642159678344 28.801263641585294 26.759333716255025 10-11 24.274674561990448 25.837179049057646 26.913213659063302 22.974932729888607 12-13 30.186566521387103 27.736050577959997 25.73773880687006 16.339644093782837 14-15 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31.382426048070904 34.29888462178747 16.519709990183753 40-41 17.233806795347288 32.72399499362721 32.89393608836937 17.14826212265613 42-43 16.120920539325585 32.474645554467926 28.903924377298264 22.500509528908232 44-45 19.72779353179569 28.095760191956646 30.503254767344405 21.67319150890325 46-47 22.94789343940033 31.940917008887702 30.92071090142599 14.190478650285973 48-49 19.60399392702674 29.868925950480584 32.38823404481258 18.1388460776801 50-51 13.380449410475148 32.1095204891808 36.478231052421656 18.031799047922387 52-53 18.609716829245194 35.20592801774732 27.13640811945576 19.047947033551733 54-55 16.163593312764586 28.457774269928965 31.492573724617923 23.88605869268853 56-57 21.17490377522582 29.370872237035844 28.68230181714853 20.771922170589804 58-59 25.565768020759176 28.74021172526236 27.84462764369532 17.849392610283143 60-61 19.744720179562687 30.073593148144962 34.674600030998675 15.507086641293677 62-63 17.249347659468196 30.926825907607338 33.125120205763565 18.698706227160905 64-65 16.18945670672035 35.470901050081935 30.247181079584102 18.092461163613613 66-67 19.40020439096087 30.588600036744438 26.631395829505788 23.379799742788904 68-69 16.550324628729214 30.079391959770145 30.94765182348923 22.422631588011413 70-71 22.32185385617464 30.98368464244518 27.612724027706843 19.08173747367334 72-73 19.76375196538625 26.606739122949975 35.54279090585656 18.086718005807214 74-75 16.12825471887608 36.88671827922432 29.154590896830364 17.830436105069243 76-77 18.215213304825724 30.005596292097746 28.93799595345033 22.841194449626194 78-79 18.682523689369994 31.22289106478013 30.65710193103171 19.437483314818163 80-81 21.45169344146564 29.32486182137507 29.13918877811641 20.084255959042878 82-83 17.852847551716913 29.278647021846393 33.957926099423915 18.910579327012776 84-85 17.913506748494434 31.75753644072175 30.469265789065776 19.85969102171804 86-87 17.424416401612145 33.19563894406871 29.56257391863496 19.817370735684182 88-89 17.31517956610123 32.389371891904304 26.33052336598868 23.96492517600579 90-91 18.589831909616976 27.097685312758337 30.242778083953336 24.06970469367135 92-93 21.78969671218089 30.664396697251924 31.1447076792338 16.40119891133339 94-95 19.128941769622354 29.32220081598902 31.54334475105444 20.005512663334184 96-97 14.299865623815593 30.55909680827849 34.44566952646751 20.6953680414384 98-99 18.56094114403226 32.52530358558413 26.964546258745674 21.949209011637926 100-101 16.996840896036762 28.3799540493969 30.924755887421025 23.69844916714532 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3092.0 1 1843.0 2 514.5 3 419.0 4 362.5 5 336.5 6 493.5 7 939.5 8 1223.0 9 1072.0 10 748.0 11 503.0 12 416.5 13 388.0 14 392.0 15 379.5 16 376.5 17 423.0 18 495.0 19 539.5 20 609.5 21 686.5 22 765.5 23 911.0 24 1063.0 25 1408.5 26 2239.0 27 3418.0 28 3715.5 29 3756.5 30 4085.0 31 4211.5 32 4260.5 33 4162.5 34 4890.0 35 5697.0 36 6051.5 37 5867.5 38 4100.5 39 2515.0 40 2086.5 41 1810.5 42 1623.0 43 1481.5 44 1437.0 45 1595.5 46 3117.5 47 11405.0 48 28124.5 49 27143.5 50 10083.5 51 2534.0 52 947.0 53 544.0 54 400.5 55 307.0 56 248.5 57 214.5 58 184.0 59 187.0 60 207.5 61 199.0 62 161.5 63 103.0 64 64.5 65 56.0 66 45.5 67 41.0 68 35.5 69 22.5 70 19.5 71 16.0 72 5.0 73 4.5 74 8.5 75 7.5 76 3.0 77 2.0 78 2.0 79 3.0 80 2.0 81 0.5 82 1.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06425297313422561 2 0.11129532846464078 3 0.07457934381651186 4 0.13653756791022942 5 0.10441108134311662 6 0.10096895778235454 7 0.12449013544756213 8 0.09752683422159243 9 0.12104801188680003 10-11 0.11445060839533937 12-13 0.09408471066083035 14-15 0.1003952705222275 16-17 0.11445060839533937 18-19 0.09523208518108439 20-21 0.08088990367790902 22-23 0.08175043456809955 24-25 0.06941615847536874 26-27 0.06281875498390807 28-29 0.07400565655638484 30-31 0.061671380463654044 32-33 0.05765556964276495 34-35 0.04991079163105024 36-37 0.06052400594340002 38-39 0.06310559861397158 40-41 0.07630040559689291 42-43 0.07486618744657537 44-45 0.060237162313336506 46-47 0.04675551170035167 48-49 0.05536082060225688 50-51 0.03470807923768437 52-53 0.05019763526111375 54-55 0.056508195122510915 56-57 0.06281875498390807 58-59 0.07056353299562276 60-61 0.06310559861397158 62-63 0.07486618744657537 64-65 0.049623948000986746 66-67 0.07744778011714694 68-69 0.06425297313422561 70-71 0.03327386108736683 72-73 0.025815926705715645 74-75 0.03671598464812892 76-77 0.050771322521240767 78-79 0.07429250018644835 80-81 0.04589498081016115 82-83 0.06740825306492419 84-85 0.07113722025574978 86-87 0.07630040559689291 88-89 0.09695314696146543 90-91 0.06826878395511471 92-93 0.0877741507994332 94-95 0.0955189288111479 96-97 0.09924789600197349 98-99 0.12907963352857824 100-101 0.12104801188680003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 174311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.76231563125678 #Duplication Level Percentage of deduplicated Percentage of total 1 78.89806858058634 16.38106602566677 2 9.82841037827084 4.081211168543581 3 3.5865270371086737 2.233938190934594 4 1.7241855709983145 1.4319234012770279 5 0.9836699731977563 1.0211633230260855 6 0.693542593462463 0.8639730137512837 7 0.5028874582078418 0.7308775694018164 8 0.32328479456218395 0.5369712754788855 9 0.2597330828106435 0.4853394220674542 >10 2.33207150949131 10.309160064482448 >50 0.39788897792268796 5.674340689916299 >100 0.37578403470476085 15.752878475827686 >500 0.024868061120167997 3.43753406268107 >1k 0.06355171175154044 28.13477061114904 >5k 0.005526235804481777 8.924852705795962 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8986 5.155153719501351 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6571 3.769698986294611 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4445 2.5500398712645787 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3380 1.9390629392293086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2998 1.719914405860789 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2845 1.6321402550613557 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2764 1.5856715869910678 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2693 1.5449397915220497 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2627 1.5070764323536667 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2554 1.4651972623643947 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2449 1.404960100051058 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2353 1.3498861230788648 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 1.121558593548313 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1914 1.0980374158831054 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1886 1.081974172599549 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1878 1.077384674518533 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1804 1.0349318172691337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1629 0.9345365467469064 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1600 0.9178996162032229 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1474 0.8456150214272191 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1365 0.7830831100733746 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1310 0.7515303107663889 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1068 0.6126979938156514 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1036 0.5943400014915868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 0.5822925690289196 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 889 0.5100079742529158 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 819 0.46984986604402473 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 766 0.439444441257293 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 697 0.3998600203085291 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 644 0.36945459552179727 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 607 0.3482281668970977 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 532 0.30520162238757165 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 526 0.3017594988268096 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 512 0.29372787718503135 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 498 0.2856962555432531 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 498 0.2856962555432531 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 496 0.28454888102299913 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 479 0.2747961976008399 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 450 0.25815926705715647 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 449 0.25758557979702945 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 445 0.25529083075652137 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 437 0.2507013326755053 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 435 0.24955395815525125 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 419 0.24037496199321903 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 413 0.2369328384324569 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 385 0.22086959514890053 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 385 0.22086959514890053 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 369 0.2116905989868683 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 357 0.2048063518653441 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 353 0.20251160282483607 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 352 0.20193791556470908 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 339 0.19447998118305787 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 331 0.18989048310204176 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.1835799232406446 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 312 0.17899042515962849 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 311 0.17841673789950147 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 303 0.17382723981848536 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 292 0.1675166799570882 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 282 0.16177980735581804 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 278 0.15948505831530999 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 274 0.15719030927480193 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 257 0.1474376258526427 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 254 0.14571656407226166 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 249 0.14284812777162656 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 244 0.1399796914709915 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 243 0.1394060042108645 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 241 0.13825862969061045 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 239 0.13711125517035644 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 236 0.1353901933899754 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.13481650612984838 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 234 0.13424281886972136 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 231 0.1325217570893403 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 226 0.12965332078870526 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 225 0.12907963352857824 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 221 0.12678488448807015 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 217 0.12449013544756213 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 216 0.12391644818743511 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 207 0.11875326284629198 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 207 0.11875326284629198 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 197 0.11301639024502183 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 196 0.11244270298489481 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 192 0.11014795394438677 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.10900057942413273 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 190 0.10900057942413273 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 189 0.10842689216400572 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 188 0.1078532049038787 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 186 0.10670583038362468 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 184 0.10555845586337063 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 181 0.1038373940829896 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 181 0.1038373940829896 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 180 0.10326370682286258 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 177 0.10154264504248153 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 175 0.1003952705222275 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 175 0.1003952705222275 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.736872601270144E-4 0.0 10-11 0.0 0.0 0.0 5.736872601270144E-4 0.0 12-13 0.0 0.0 0.0 5.736872601270144E-4 0.0 14-15 0.0 0.0 0.0 0.0011473745202540287 0.0 16-17 0.0 0.0 0.0 0.0028684363006350718 0.0 18-19 0.0 0.0 0.0 0.003442123560762086 0.0 20-21 0.0 0.0 0.0 0.0037289671908255933 0.0 22-23 0.0 0.0 0.0 0.004589498081016115 0.0 24-25 0.0 0.0 0.0 0.007457934381651187 0.0 26-27 0.0 0.0 0.0 0.01032637068228626 0.0 28-29 0.0 0.0 0.0 0.022947490405080574 0.0 30-31 0.0 0.0 0.0 0.04417391902978011 0.0 32-33 0.0 0.0 0.0 0.07486618744657537 0.0 34-35 0.0 0.0 0.0 0.10900057942413273 0.0 36-37 0.0 0.0 0.0 0.15001921852321426 0.0 38-39 0.0 0.0 0.0 0.20910900631629675 0.0 40-41 0.0 0.0 0.0 0.2644698269185536 0.0 42-43 0.0 0.0 0.0 0.31294640039928634 0.0 44-45 0.0 0.0 0.0 0.34708079237684364 0.0 46-47 0.0 0.0 0.0 0.3906810241464968 0.0 48-49 0.0 0.0 0.0 0.4225206670835461 0.0 50-51 0.0 0.0 0.0 0.45780243358135747 0.0 52-53 0.0 0.0 0.0 0.538405493629203 0.0 54-55 0.0 0.0 0.0 0.6703535634584163 0.0 56-57 0.0 0.0 0.0 0.8806099442949671 0.0 58-59 0.0 0.0 0.0 1.0725083328074534 0.0 60-61 0.0 0.0 0.0 1.3151780438411804 0.0 62-63 0.0 0.0 0.0 1.4525761426416004 0.0 64-65 0.0 0.0 0.0 1.6186586044483708 0.0 66-67 0.0 0.0 0.0 1.949102466281531 0.0 68-69 0.0 0.0 0.0 2.42009970684581 0.0 70-71 0.0 0.0 0.0 2.831146628726816 0.0 72-73 0.0 0.0 0.0 3.2542409830704893 0.0 74-75 0.0 0.0 0.0 3.7174934456230533 0.0 76-77 0.0 0.0 0.0 4.057689990878373 0.0 78-79 0.0 0.0 0.0 4.467302694609061 0.0 80-81 0.0 0.0 0.0 4.812662425205524 0.0 82-83 0.0 0.0 0.0 5.178388053536495 0.0 84-85 0.0 0.0 0.0 5.865665391168658 0.0 86-87 0.0 0.0 0.0 6.65018271939235 0.0 88-89 0.0 0.0 0.0 7.599061447642432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGCA 125 0.0 49.453377 4 GGCATTC 135 0.0 49.29831 7 GAGGATT 20 5.3848204E-4 47.4967 90-91 AGGCATT 135 0.0 45.777 6 TAGGCAT 135 0.0 45.777 5 CCTTTAG 125 0.0 45.62303 1 TGGGACA 45 9.565153E-4 42.25569 6 GCTTTGC 185 0.0 41.101833 1 CATTCCG 165 0.0 40.33498 9 CTTTAGG 150 0.0 38.019196 2 CTTTGCG 205 0.0 37.0919 2 TTGCGTT 210 0.0 36.22958 4 TTTGCGT 210 0.0 36.22958 3 GCATTCC 185 0.0 35.97444 8 ATGGGGA 80 1.0665559E-5 35.653244 5 ATGGGAC 95 3.4552766E-5 30.02378 5 GCTTGAT 95 3.4620676E-5 30.015156 1 TGGGATA 80 4.4574164E-4 29.711037 6 CGCAGAA 145 2.3572284E-8 29.506128 9 CTTGATA 100 4.9123762E-5 28.514397 2 >>END_MODULE